Click here to switch to the map view.

The map label for this gene is murD

Identifier: 158334692

GI number: 158334692

Start: 1508152

End: 1509537

Strand: Reverse

Name: murD

Synonym: AM1_1525

Alternate gene names: 158334692

Gene position: 1509537-1508152 (Counterclockwise)

Preceding gene: 158334693

Following gene: 158334691

Centisome position: 23.21

GC content: 52.96

Gene sequence:

>1386_bases
ATGCCTGCTGTTCATATTATTGGTCTTGGTCGCTCCGGTATCGCGGCTGCTCGTCTGCTGCGCCGTGAGGGGTGGCAAGT
TTCGGTGGGTGATGCAGGTCATTCAGAAGGGTTAGTGGCGACGCAGAAAACCTTGGCGGCAGAAGGCATCCCCGTCCATT
TAAATCTGAAATTTTCCCTCTCAGCCTTGGCTGAACTCAGTCTGGACCGTCCTGATCGGATTGTGGTCAGTCCTGGAGTG
CCGTGGCAGTTGCCTGCATTAGAAGAGGCACGATCTCAAGGCATTGAGACCTTTGGTGAACTTGAACTGGCCTGGCGATC
CCTCAAGCAATATCCTTGGGTCGGGATTACGGGCACTAACGGCAAAACCACCACGACTGCCATTGTCGAAGCCATCTTTA
AGGCGGCAGGGTTACGGGCACCCGCCTGCGGCAATATTGGCTATAGCGCCTGTGAATTGGCCTTAGCAGGAGATCCTCTG
GATTGGGTGATTGCGGAGGTGAGCAGCTACCAAATTGAATCCTCCCCCACCCTAGCCCCTACCATTGGCGTCTGGACGAC
CTTCTCTCCCGACCACTTGGAGCGTCATGGCACTGTTGAGTGCTATCGCGAAATTAAGGCCCTACTTCTCAATCAGTCCA
AACAGAAGATTCTCAATGGTGATGATCAGGGCTTGATCAAAGGATTGCCTTGGCGATGGGCTGATGCCTATTGGACGAGT
AGCCATGGCCCCAGCTTGCCGGATGCCTGTAATGGTCAACGCTGTCCAGTGCCCAATATTTTTATTGAAGAGGGATGGGT
GAAGGTGGAAGGAGAACCCATTGTGCAGGTATCCTCCATGCGGATGTTGGGGGTTCATAACCAGCAGAATCTTTTACTGG
CAGTGGCTGTGGCTCATTTGGCGGGGATTGGGAAGGATGCGATCGCAACTGCCGTATCCACGTTCCCCGGTGTGGCCCAT
CGCCTGGAACATATCTGCACCTGGCAGGGCGTAGATTTTATTAATGACAGCAAGGCCACGAACTATGAAGCAGCCCAAAC
GGGATTAGCTGCAGTCAATAGTCCTGCCATTCTGATTGCAGGTGGGGAAGCCAAAGTGGGTGACGATCAGGCATGGCTGC
AAACCATTCAGGCTCAAGCAGCCAAGGTGCTGTTGATTGGTAGTGCTGCCGGTGCCTTTAGCCAGCGCCTTAAGGCGATT
GGGTATGACGATTACGAAATCGTGGACACAATGAACAAAGCGGTGACTAGAGCCGCTGAATTGGCCCCTTCTCTGCAAGC
TAAAAGTGTGCTCTTATCACCTGCCTGTGCTAGCTTTGACCAATATCAAAACTTTGAGAGACGAGGCGATCATTTTCGGC
AACTATGCCAGGATCTCTCGCAATAA

Upstream 100 bases:

>100_bases
GACCTACAAGATTTTAATTTATCCCTAAGAAGCATTTAATTGGAGCAAAGGGACCACCAAGCGATACGATCAGGATTGCT
GAATCACGTTGCTCCCTTCT

Downstream 100 bases:

>100_bases
TTTCTGAAGGCAAAACGAGCTACCAAACTATTTCGTGTTGAGAATCAACTTTGTAACTGTGGGTATCCGACGATAACTTG
CGATCAGTTTTCTATGAACA

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 461; Mature: 460

Protein sequence:

>461_residues
MPAVHIIGLGRSGIAAARLLRREGWQVSVGDAGHSEGLVATQKTLAAEGIPVHLNLKFSLSALAELSLDRPDRIVVSPGV
PWQLPALEEARSQGIETFGELELAWRSLKQYPWVGITGTNGKTTTTAIVEAIFKAAGLRAPACGNIGYSACELALAGDPL
DWVIAEVSSYQIESSPTLAPTIGVWTTFSPDHLERHGTVECYREIKALLLNQSKQKILNGDDQGLIKGLPWRWADAYWTS
SHGPSLPDACNGQRCPVPNIFIEEGWVKVEGEPIVQVSSMRMLGVHNQQNLLLAVAVAHLAGIGKDAIATAVSTFPGVAH
RLEHICTWQGVDFINDSKATNYEAAQTGLAAVNSPAILIAGGEAKVGDDQAWLQTIQAQAAKVLLIGSAAGAFSQRLKAI
GYDDYEIVDTMNKAVTRAAELAPSLQAKSVLLSPACASFDQYQNFERRGDHFRQLCQDLSQ

Sequences:

>Translated_461_residues
MPAVHIIGLGRSGIAAARLLRREGWQVSVGDAGHSEGLVATQKTLAAEGIPVHLNLKFSLSALAELSLDRPDRIVVSPGV
PWQLPALEEARSQGIETFGELELAWRSLKQYPWVGITGTNGKTTTTAIVEAIFKAAGLRAPACGNIGYSACELALAGDPL
DWVIAEVSSYQIESSPTLAPTIGVWTTFSPDHLERHGTVECYREIKALLLNQSKQKILNGDDQGLIKGLPWRWADAYWTS
SHGPSLPDACNGQRCPVPNIFIEEGWVKVEGEPIVQVSSMRMLGVHNQQNLLLAVAVAHLAGIGKDAIATAVSTFPGVAH
RLEHICTWQGVDFINDSKATNYEAAQTGLAAVNSPAILIAGGEAKVGDDQAWLQTIQAQAAKVLLIGSAAGAFSQRLKAI
GYDDYEIVDTMNKAVTRAAELAPSLQAKSVLLSPACASFDQYQNFERRGDHFRQLCQDLSQ
>Mature_460_residues
PAVHIIGLGRSGIAAARLLRREGWQVSVGDAGHSEGLVATQKTLAAEGIPVHLNLKFSLSALAELSLDRPDRIVVSPGVP
WQLPALEEARSQGIETFGELELAWRSLKQYPWVGITGTNGKTTTTAIVEAIFKAAGLRAPACGNIGYSACELALAGDPLD
WVIAEVSSYQIESSPTLAPTIGVWTTFSPDHLERHGTVECYREIKALLLNQSKQKILNGDDQGLIKGLPWRWADAYWTSS
HGPSLPDACNGQRCPVPNIFIEEGWVKVEGEPIVQVSSMRMLGVHNQQNLLLAVAVAHLAGIGKDAIATAVSTFPGVAHR
LEHICTWQGVDFINDSKATNYEAAQTGLAAVNSPAILIAGGEAKVGDDQAWLQTIQAQAAKVLLIGSAAGAFSQRLKAIG
YDDYEIVDTMNKAVTRAAELAPSLQAKSVLLSPACASFDQYQNFERRGDHFRQLCQDLSQ

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=394, Percent_Identity=35.5329949238579, Blast_Score=184, Evalue=1e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_ACAM1 (B0C915)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001515864.1
- ProteinModelPortal:   B0C915
- SMR:   B0C915
- GeneID:   5680345
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_1525
- HOGENOM:   HBG750024
- OMA:   VKYYNDS
- ProtClustDB:   PRK02705
- BioCyc:   AMAR329726:AM1_1525-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 49515; Mature: 49384

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAVHIIGLGRSGIAAARLLRREGWQVSVGDAGHSEGLVATQKTLAAEGIPVHLNLKFSL
CCCEEEEECCCCHHHHHHHHHHCCCEEEECCCCCCCCCEEHHHHHHHCCCEEEEEEEECH
SALAELSLDRPDRIVVSPGVPWQLPALEEARSQGIETFGELELAWRSLKQYPWVGITGTN
HHHHHHCCCCCCEEEECCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEECCC
GKTTTTAIVEAIFKAAGLRAPACGNIGYSACELALAGDPLDWVIAEVSSYQIESSPTLAP
CCHHHHHHHHHHHHHCCCCCCCCCCCCHHHEEEEECCCCHHHHHHHHHHEEECCCCCCCC
TIGVWTTFSPDHLERHGTVECYREIKALLLNQSKQKILNGDDQGLIKGLPWRWADAYWTS
EECEEECCCHHHHHHCCHHHHHHHHHHHHHCCCHHHHHCCCCCHHHCCCCCCCCCCCEEC
SHGPSLPDACNGQRCPVPNIFIEEGWVKVEGEPIVQVSSMRMLGVHNQQNLLLAVAVAHL
CCCCCCCCCCCCCCCCCCCEEEECCEEEECCCCEEEEECCCEEECCCCCCHHHHHHHHHH
AGIGKDAIATAVSTFPGVAHRLEHICTWQGVDFINDSKATNYEAAQTGLAAVNSPAILIA
HCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCHHHCCCCEEEEE
GGEAKVGDDQAWLQTIQAQAAKVLLIGSAAGAFSQRLKAIGYDDYEIVDTMNKAVTRAAE
CCCCCCCCHHHHHHHHHHHHCEEEEEECCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
LAPSLQAKSVLLSPACASFDQYQNFERRGDHFRQLCQDLSQ
HCCCHHHHHHEECHHHHCHHHHHHHHHCCHHHHHHHHHHCC
>Mature Secondary Structure 
PAVHIIGLGRSGIAAARLLRREGWQVSVGDAGHSEGLVATQKTLAAEGIPVHLNLKFSL
CCEEEEECCCCHHHHHHHHHHCCCEEEECCCCCCCCCEEHHHHHHHCCCEEEEEEEECH
SALAELSLDRPDRIVVSPGVPWQLPALEEARSQGIETFGELELAWRSLKQYPWVGITGTN
HHHHHHCCCCCCEEEECCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEECCC
GKTTTTAIVEAIFKAAGLRAPACGNIGYSACELALAGDPLDWVIAEVSSYQIESSPTLAP
CCHHHHHHHHHHHHHCCCCCCCCCCCCHHHEEEEECCCCHHHHHHHHHHEEECCCCCCCC
TIGVWTTFSPDHLERHGTVECYREIKALLLNQSKQKILNGDDQGLIKGLPWRWADAYWTS
EECEEECCCHHHHHHCCHHHHHHHHHHHHHCCCHHHHHCCCCCHHHCCCCCCCCCCCEEC
SHGPSLPDACNGQRCPVPNIFIEEGWVKVEGEPIVQVSSMRMLGVHNQQNLLLAVAVAHL
CCCCCCCCCCCCCCCCCCCEEEECCEEEECCCCEEEEECCCEEECCCCCCHHHHHHHHHH
AGIGKDAIATAVSTFPGVAHRLEHICTWQGVDFINDSKATNYEAAQTGLAAVNSPAILIA
HCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCHHHCCCCEEEEE
GGEAKVGDDQAWLQTIQAQAAKVLLIGSAAGAFSQRLKAIGYDDYEIVDTMNKAVTRAAE
CCCCCCCCHHHHHHHHHHHHCEEEEEECCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
LAPSLQAKSVLLSPACASFDQYQNFERRGDHFRQLCQDLSQ
HCCCHHHHHHEECHHHHCHHHHHHHHHCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA