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The map label for this gene is pheS

Identifier: 158334545

GI number: 158334545

Start: 1349070

End: 1350062

Strand: Direct

Name: pheS

Synonym: AM1_1373

Alternate gene names: 158334545

Gene position: 1349070-1350062 (Clockwise)

Preceding gene: 158334544

Following gene: 158334547

Centisome position: 20.74

GC content: 50.25

Gene sequence:

>993_bases
ATGACCAGTGATCTGGAAACTCAGTTGCAACAGCTTCAAACAGAGGCTGTTGCAGCTATAACCTCTGCTGATACCCTAGA
TGCCTTAGAAACACTGCGGGTGAGCTATTTGGGTAAAAAAGGCCAGTTGTCTCAGATCCTTAAGGGTATGGGCAAGCTGG
ATGCATCTGAACGGCCCAAGATTGGGGGATTAGCCAATCAGGTCAAGGAGGCGCTTCAGCAGGGGCTAGAACAGAAAAAA
TCGGATCTAAACCAGGCGGCGATTGCGGCTCAACTGGCAGCAGAGACCTTAGATGTGACCATGCCTGGAACTTATATTCC
TCAAGGGCATATTCATCCCCTGAATAGCACGATCGATAAAGCGTTGGATATTTTTGTGGGCCTAGGGTACACCATTGCCC
AAGGTCCCGAGATGGAAACGGACTACTACAACTTTGAAGCCCTGAATACCCCGCCCGATCACCCAGCGCGGGATATGCAA
GATACCTTTTATCTCCCGGATGGCAATCTGCTCCGCACCCATACTTCTGCGGTTCAGATTCATTACATGGAAGACCATGA
ACCACCCATTCGGATCGCAGCCCCTGGGCGCTGTTACCGTCGGGATACAGAAGACGCTACCCATGCTGCCGTATTCCATC
AGATTGAGATTTTAGCAGTGGATAAGGGGCTGACCTTTACGGATCTCAAAGGCACGATTAAAGTCTTTATTGAACAAATG
TTTGGGGATGTACCCATCCGATTTCGAGCGAGTTATTTCCCCTTTACTGAACCCTCTGCTGAGGTGGATGTGCAGTGGAA
AGGCCGATGGCTTGAGGTCTTGGGGTGCGGCATGGTTGATCCCAATGTCCTCAAAAACGTGGGCTATGATCCTGAAGTCT
ATACTGGGTTCGCCGCAGGCTTCGGGGTGGAGCGATTTGCGATGGTCCTTCACCAGATTGACGATATTCGGCGTTTGTAC
ACTAGCGATTTGCGCTTCTTGCGTCAATTTTAG

Upstream 100 bases:

>100_bases
AGAAGCCTAATTACTTCCTCCTTGTGGGTACTGCTTTCCTGAATCTTTCATCCATGCTGATCCCAATGCGGATCAGCAAT
TCTATATTCAACGAAACGTC

Downstream 100 bases:

>100_bases
GCATTGCCCAAGCTATGAGTATTGGGGTAGCTGGAGTCCAGTTGAAGGGTGTATTGATGGTGAGGATGCCGATGTGCGAT
CGCATTTCTCCCCGACCCAT

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 330; Mature: 329

Protein sequence:

>330_residues
MTSDLETQLQQLQTEAVAAITSADTLDALETLRVSYLGKKGQLSQILKGMGKLDASERPKIGGLANQVKEALQQGLEQKK
SDLNQAAIAAQLAAETLDVTMPGTYIPQGHIHPLNSTIDKALDIFVGLGYTIAQGPEMETDYYNFEALNTPPDHPARDMQ
DTFYLPDGNLLRTHTSAVQIHYMEDHEPPIRIAAPGRCYRRDTEDATHAAVFHQIEILAVDKGLTFTDLKGTIKVFIEQM
FGDVPIRFRASYFPFTEPSAEVDVQWKGRWLEVLGCGMVDPNVLKNVGYDPEVYTGFAAGFGVERFAMVLHQIDDIRRLY
TSDLRFLRQF

Sequences:

>Translated_330_residues
MTSDLETQLQQLQTEAVAAITSADTLDALETLRVSYLGKKGQLSQILKGMGKLDASERPKIGGLANQVKEALQQGLEQKK
SDLNQAAIAAQLAAETLDVTMPGTYIPQGHIHPLNSTIDKALDIFVGLGYTIAQGPEMETDYYNFEALNTPPDHPARDMQ
DTFYLPDGNLLRTHTSAVQIHYMEDHEPPIRIAAPGRCYRRDTEDATHAAVFHQIEILAVDKGLTFTDLKGTIKVFIEQM
FGDVPIRFRASYFPFTEPSAEVDVQWKGRWLEVLGCGMVDPNVLKNVGYDPEVYTGFAAGFGVERFAMVLHQIDDIRRLY
TSDLRFLRQF
>Mature_329_residues
TSDLETQLQQLQTEAVAAITSADTLDALETLRVSYLGKKGQLSQILKGMGKLDASERPKIGGLANQVKEALQQGLEQKKS
DLNQAAIAAQLAAETLDVTMPGTYIPQGHIHPLNSTIDKALDIFVGLGYTIAQGPEMETDYYNFEALNTPPDHPARDMQD
TFYLPDGNLLRTHTSAVQIHYMEDHEPPIRIAAPGRCYRRDTEDATHAAVFHQIEILAVDKGLTFTDLKGTIKVFIEQMF
GDVPIRFRASYFPFTEPSAEVDVQWKGRWLEVLGCGMVDPNVLKNVGYDPEVYTGFAAGFGVERFAMVLHQIDDIRRLYT
SDLRFLRQF

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily

Homologues:

Organism=Homo sapiens, GI5729820, Length=227, Percent_Identity=35.2422907488987, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI4758340, Length=258, Percent_Identity=33.3333333333333, Blast_Score=129, Evalue=3e-30,
Organism=Escherichia coli, GI1788007, Length=326, Percent_Identity=53.3742331288344, Blast_Score=351, Evalue=5e-98,
Organism=Caenorhabditis elegans, GI32566635, Length=244, Percent_Identity=35.655737704918, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI32563657, Length=268, Percent_Identity=29.4776119402985, Blast_Score=124, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI17508957, Length=268, Percent_Identity=29.4776119402985, Blast_Score=124, Evalue=7e-29,
Organism=Saccharomyces cerevisiae, GI6321087, Length=285, Percent_Identity=30.1754385964912, Blast_Score=134, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6325304, Length=163, Percent_Identity=33.7423312883436, Blast_Score=101, Evalue=2e-22,
Organism=Drosophila melanogaster, GI18858079, Length=260, Percent_Identity=31.1538461538462, Blast_Score=118, Evalue=4e-27,
Organism=Drosophila melanogaster, GI17137424, Length=278, Percent_Identity=30.2158273381295, Blast_Score=112, Evalue=3e-25,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_ACAM1 (B0C6I2)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001515717.1
- ProteinModelPortal:   B0C6I2
- SMR:   B0C6I2
- GeneID:   5680193
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_1373
- HOGENOM:   HBG284353
- OMA:   FRASYFP
- ProtClustDB:   PRK00488
- BioCyc:   AMAR329726:AM1_1373-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00281
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d; SSF46589 tRNA_binding_arm

EC number: =6.1.1.20

Molecular weight: Translated: 36839; Mature: 36708

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSDLETQLQQLQTEAVAAITSADTLDALETLRVSYLGKKGQLSQILKGMGKLDASERPK
CCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCC
IGGLANQVKEALQQGLEQKKSDLNQAAIAAQLAAETLDVTMPGTYIPQGHIHPLNSTIDK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCHHHHHH
ALDIFVGLGYTIAQGPEMETDYYNFEALNTPPDHPARDMQDTFYLPDGNLLRTHTSAVQI
HHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCEEECCCCCEEEEECCEEEE
HYMEDHEPPIRIAAPGRCYRRDTEDATHAAVFHQIEILAVDKGLTFTDLKGTIKVFIEQM
EEECCCCCCEEEECCCHHHCCCCCHHHHHHHHHEEEEEEECCCCEEHHHHHHHHHHHHHH
FGDVPIRFRASYFPFTEPSAEVDVQWKGRWLEVLGCGMVDPNVLKNVGYDPEVYTGFAAG
HCCCCEEEEECCCCCCCCCCCEEEEECCCEEEEHCCCCCCHHHHHHCCCCCHHHHHHHHH
FGVERFAMVLHQIDDIRRLYTSDLRFLRQF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TSDLETQLQQLQTEAVAAITSADTLDALETLRVSYLGKKGQLSQILKGMGKLDASERPK
CCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCC
IGGLANQVKEALQQGLEQKKSDLNQAAIAAQLAAETLDVTMPGTYIPQGHIHPLNSTIDK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCHHHHHH
ALDIFVGLGYTIAQGPEMETDYYNFEALNTPPDHPARDMQDTFYLPDGNLLRTHTSAVQI
HHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCEEECCCCCEEEEECCEEEE
HYMEDHEPPIRIAAPGRCYRRDTEDATHAAVFHQIEILAVDKGLTFTDLKGTIKVFIEQM
EEECCCCCCEEEECCCHHHCCCCCHHHHHHHHHEEEEEEECCCCEEHHHHHHHHHHHHHH
FGDVPIRFRASYFPFTEPSAEVDVQWKGRWLEVLGCGMVDPNVLKNVGYDPEVYTGFAAG
HCCCCEEEEECCCCCCCCCCCEEEEECCCEEEEHCCCCCCHHHHHHCCCCCHHHHHHHHH
FGVERFAMVLHQIDDIRRLYTSDLRFLRQF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA