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The map label for this gene is ndhK

Identifier: 158334544

GI number: 158334544

Start: 1348281

End: 1348979

Strand: Direct

Name: ndhK

Synonym: AM1_1371

Alternate gene names: 158334544

Gene position: 1348281-1348979 (Clockwise)

Preceding gene: 158334541

Following gene: 158334545

Centisome position: 20.73

GC content: 49.93

Gene sequence:

>699_bases
ATGCAGGACCAACAGATAATTAATCCAATTTCTCAATCTCAGATCCCTCAAGAGCTGTCTGAGAATGTGATTTTGTCTTC
CGTGAGTGATCTCTACGATTGGGCAAGACTCTCTAGCCTTTGGCCCTTAATGTATGGAACTGCTTGCTGCTTTATTGAAA
TCGCAGCCATGATTGGCTCTCGATTCGATTTTGATCGCTTTGGTTTGGTGCCCCGTTCTAGCCCCCGACAAGCTGACTTA
ATCATTACGGCTGGGACAGTGACGATGAAAATGGCACCTGCACTGGTGCGACTCTATGAACAAATGCCAGATCCGAAATA
TGTGATTGCGATGGGTGCTTGTACCATCACAGGCGGTATGTTCAGCAGCGACTCTACCACTGCTGTCCGCGGGGTCGACA
AGCTTATTCCTGTTGATGTCTATTTGCCTGGCTGCCCGCCTCGTCCTGAAGCAGTGATGGATGCCATTGTTAAGCTACGC
AAAAAGATCGCCAATGAGGATGTGCGTGAGCGGGGCAATTTGATGCAGACCCATCGCTATTACAGCACAACCCATCAGAT
GAAAGTGGTCCCCCCCATTCACACTGGTGTCTATCTGGAGGCCGCTGCGCGTAAATCTCCTGCGGCTGCTTTAGGTGCGG
GTGTGGGTGAAGATATGACACCTGCATTGGTAGCTGAAGCTGAGAAGGAAGAAGCCTAA

Upstream 100 bases:

>100_bases
TATTCTTCGCACTATTTGATATCCTCCCAGATGCTGTTCAGTAATTAGCTGCAAGCATTTCAGTTCATTAAATTAGCAAA
CATTGAGGGCCCTTTCTGCA

Downstream 100 bases:

>100_bases
TTACTTCCTCCTTGTGGGTACTGCTTTCCTGAATCTTTCATCCATGCTGATCCCAATGCGGATCAGCAATTCTATATTCA
ACGAAACGTCATGACCAGTG

Product: NADH dehydrogenase subunit B

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase I subunit K 1; NDH-1 subunit K 1; NDH-K 1

Number of amino acids: Translated: 232; Mature: 232

Protein sequence:

>232_residues
MQDQQIINPISQSQIPQELSENVILSSVSDLYDWARLSSLWPLMYGTACCFIEIAAMIGSRFDFDRFGLVPRSSPRQADL
IITAGTVTMKMAPALVRLYEQMPDPKYVIAMGACTITGGMFSSDSTTAVRGVDKLIPVDVYLPGCPPRPEAVMDAIVKLR
KKIANEDVRERGNLMQTHRYYSTTHQMKVVPPIHTGVYLEAAARKSPAAALGAGVGEDMTPALVAEAEKEEA

Sequences:

>Translated_232_residues
MQDQQIINPISQSQIPQELSENVILSSVSDLYDWARLSSLWPLMYGTACCFIEIAAMIGSRFDFDRFGLVPRSSPRQADL
IITAGTVTMKMAPALVRLYEQMPDPKYVIAMGACTITGGMFSSDSTTAVRGVDKLIPVDVYLPGCPPRPEAVMDAIVKLR
KKIANEDVRERGNLMQTHRYYSTTHQMKVVPPIHTGVYLEAAARKSPAAALGAGVGEDMTPALVAEAEKEEA
>Mature_232_residues
MQDQQIINPISQSQIPQELSENVILSSVSDLYDWARLSSLWPLMYGTACCFIEIAAMIGSRFDFDRFGLVPRSSPRQADL
IITAGTVTMKMAPALVRLYEQMPDPKYVIAMGACTITGGMFSSDSTTAVRGVDKLIPVDVYLPGCPPRPEAVMDAIVKLR
KKIANEDVRERGNLMQTHRYYSTTHQMKVVPPIHTGVYLEAAARKSPAAALGAGVGEDMTPALVAEAEKEEA

Specific function: NDH-1 shuttles electrons from an unknown electron donor, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory and/or the photosynthetic chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cellular thylakoid membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family

Homologues:

Organism=Homo sapiens, GI187281616, Length=143, Percent_Identity=51.0489510489511, Blast_Score=172, Evalue=3e-43,
Organism=Escherichia coli, GI1788624, Length=167, Percent_Identity=47.3053892215569, Blast_Score=170, Evalue=8e-44,
Organism=Escherichia coli, GI1789074, Length=108, Percent_Identity=44.4444444444444, Blast_Score=97, Evalue=6e-22,
Organism=Escherichia coli, GI1788834, Length=129, Percent_Identity=41.0852713178295, Blast_Score=97, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI17509685, Length=161, Percent_Identity=45.3416149068323, Blast_Score=159, Evalue=2e-39,
Organism=Drosophila melanogaster, GI18859983, Length=139, Percent_Identity=51.7985611510791, Blast_Score=168, Evalue=2e-42,
Organism=Drosophila melanogaster, GI24642371, Length=139, Percent_Identity=51.7985611510791, Blast_Score=168, Evalue=2e-42,
Organism=Drosophila melanogaster, GI24651058, Length=147, Percent_Identity=48.2993197278912, Blast_Score=164, Evalue=5e-41,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NDHK1_ACAM1 (B0C6I1)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001515716.1
- ProteinModelPortal:   B0C6I1
- SMR:   B0C6I1
- GeneID:   5680192
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_1371
- HOGENOM:   HBG553221
- OMA:   ITMKMAP
- ProtClustDB:   PRK06411
- BioCyc:   AMAR329726:AM1_1371-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_01356
- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406
- PANTHER:   PTHR11995:SF2
- PANTHER:   PTHR11995
- TIGRFAMs:   TIGR01957

Pfam domain/function: PF01058 Oxidored_q6

EC number: 1.6.5.3

Molecular weight: Translated: 25380; Mature: 25380

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDQQIINPISQSQIPQELSENVILSSVSDLYDWARLSSLWPLMYGTACCFIEIAAMIGS
CCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
RFDFDRFGLVPRSSPRQADLIITAGTVTMKMAPALVRLYEQMPDPKYVIAMGACTITGGM
CCCCHHHCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEECCC
FSSDSTTAVRGVDKLIPVDVYLPGCPPRPEAVMDAIVKLRKKIANEDVRERGNLMQTHRY
CCCCCCHHHHHHHHHCEEEEEECCCCCCHHHHHHHHHHHHHHHHCHHHHHHCCHHHHHHH
YSTTHQMKVVPPIHTGVYLEAAARKSPAAALGAGVGEDMTPALVAEAEKEEA
HHHCCCEEECCCCCCCEEEEHHHCCCCHHHHHCCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MQDQQIINPISQSQIPQELSENVILSSVSDLYDWARLSSLWPLMYGTACCFIEIAAMIGS
CCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
RFDFDRFGLVPRSSPRQADLIITAGTVTMKMAPALVRLYEQMPDPKYVIAMGACTITGGM
CCCCHHHCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEECCC
FSSDSTTAVRGVDKLIPVDVYLPGCPPRPEAVMDAIVKLRKKIANEDVRERGNLMQTHRY
CCCCCCHHHHHHHHHCEEEEEECCCCCCHHHHHHHHHHHHHHHHCHHHHHHCCHHHHHHH
YSTTHQMKVVPPIHTGVYLEAAARKSPAAALGAGVGEDMTPALVAEAEKEEA
HHHCCCEEECCCCCCCEEEEHHHCCCCHHHHHCCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA