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The map label for this gene is prfA

Identifier: 158334426

GI number: 158334426

Start: 1232853

End: 1233944

Strand: Reverse

Name: prfA

Synonym: AM1_1247

Alternate gene names: 158334426

Gene position: 1233944-1232853 (Counterclockwise)

Preceding gene: 158334427

Following gene: 158334425

Centisome position: 18.97

GC content: 51.65

Gene sequence:

>1092_bases
ATGGCCGAAGCCTATCTGCTTGAAAAGCTGAAATCCGTTGAGCAAACGTTCCATGAGTTGACTCGTCGCCTCGGTGACCC
GGAGGTGGCCACGAATCCGACTGAGTTTCAGGAAGTTGCAAAGGCAAGAGCGTCTTTAGAAGAGACGGTCAACACGTTTG
AGGACTGGAAAGCTACTCAACAGGAGTTAGCCGATGCTCAACAGATGGCCAAAGATGGGGATGAAGATCCAGAGCTAAAG
GAGATGGCGGCGATGGAAGCCGCTGAGTTGACCGAAAAACTGGATGGCCTGGAAACAAAACTGAAAGTACTGCTGCTGCC
TCGGGATCCCAACGATGAGAAAAACATTATGTTGGAGATCAGAGCCGGGGCTGGTGGTGATGAAGCTAGTATTTGGGCTG
GAGATTTAGTTCGCATTTACTCTAAGTATGCGGAAAGCCAAAAGTGGCGGGTGAAGCTGCTGAGTGAGTCCGGTGGAGAG
ATGGGGGGCTTTAAAGAAGCTATCCTCGAAATCCAGGGTGAGCGGGTTTATAGCAAGCTTAAGTATGAGGCGGGCGTGCA
TCGGGTGCAGCGGGTACCGGCAACGGAGGCCTCGGGTCGAGTCCATACGTCCACGGCGACGGTGGCTGTCATGCCGGAAG
TGGATGATGTTGAGATTGAGATTGACCCCAAAGATATTGAAATGTCCACGGCCCGGTCTGGTGGTGCTGGTGGACAAAAT
GTGAACAAGGTGGAGACGGCGGTTGATTTGGTCCACAAACCAACGGGGATCCGGATCTTCTGTACGGAAGAGCGATCGCA
ACTGCAAAACAAAGAGCGAGCGATGCAAATTCTGCGAGCCAAGCTCTATGAAATGGAACTGCAAGAACAGCAAGCATCAG
TGACCTCTATGCGTCGGTCCCAGGTGGGAACGGGGGCCAGATCTGAGAAAATACGCACCTATAATTACAAAGATAATCGC
GCCACGGATCATCGTCTTAACCAAAACTTTCCCCTAGAGAAGGTATTGGTAGGGGATATTGAGCTGATTTTAGAGACCTG
TATCGCTCAAGATCAGCAGGCTCAGCTAGCAGACCTGGCAGCAGCGGTTTAA

Upstream 100 bases:

>100_bases
AGGCGCAGGCGGTAAAGGTAACGCTAAAAAGAAAGACGAGAAGTAGGACAGCTAAATCTAAGCTACCTTGTCTTCTTGTC
CTTTCCCCTAGTACTGATCC

Downstream 100 bases:

>100_bases
GCCTGCTCGATGCGCCATTCCTTTGGGGAAATCTAAAGGTTGGGAACAGAATCGGTCTTTTTCTATTCGCTAGAATAGAA
TAGCCATTCTAAAACTTGGC

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 363; Mature: 362

Protein sequence:

>363_residues
MAEAYLLEKLKSVEQTFHELTRRLGDPEVATNPTEFQEVAKARASLEETVNTFEDWKATQQELADAQQMAKDGDEDPELK
EMAAMEAAELTEKLDGLETKLKVLLLPRDPNDEKNIMLEIRAGAGGDEASIWAGDLVRIYSKYAESQKWRVKLLSESGGE
MGGFKEAILEIQGERVYSKLKYEAGVHRVQRVPATEASGRVHTSTATVAVMPEVDDVEIEIDPKDIEMSTARSGGAGGQN
VNKVETAVDLVHKPTGIRIFCTEERSQLQNKERAMQILRAKLYEMELQEQQASVTSMRRSQVGTGARSEKIRTYNYKDNR
ATDHRLNQNFPLEKVLVGDIELILETCIAQDQQAQLADLAAAV

Sequences:

>Translated_363_residues
MAEAYLLEKLKSVEQTFHELTRRLGDPEVATNPTEFQEVAKARASLEETVNTFEDWKATQQELADAQQMAKDGDEDPELK
EMAAMEAAELTEKLDGLETKLKVLLLPRDPNDEKNIMLEIRAGAGGDEASIWAGDLVRIYSKYAESQKWRVKLLSESGGE
MGGFKEAILEIQGERVYSKLKYEAGVHRVQRVPATEASGRVHTSTATVAVMPEVDDVEIEIDPKDIEMSTARSGGAGGQN
VNKVETAVDLVHKPTGIRIFCTEERSQLQNKERAMQILRAKLYEMELQEQQASVTSMRRSQVGTGARSEKIRTYNYKDNR
ATDHRLNQNFPLEKVLVGDIELILETCIAQDQQAQLADLAAAV
>Mature_362_residues
AEAYLLEKLKSVEQTFHELTRRLGDPEVATNPTEFQEVAKARASLEETVNTFEDWKATQQELADAQQMAKDGDEDPELKE
MAAMEAAELTEKLDGLETKLKVLLLPRDPNDEKNIMLEIRAGAGGDEASIWAGDLVRIYSKYAESQKWRVKLLSESGGEM
GGFKEAILEIQGERVYSKLKYEAGVHRVQRVPATEASGRVHTSTATVAVMPEVDDVEIEIDPKDIEMSTARSGGAGGQNV
NKVETAVDLVHKPTGIRIFCTEERSQLQNKERAMQILRAKLYEMELQEQQASVTSMRRSQVGTGARSEKIRTYNYKDNRA
TDHRLNQNFPLEKVLVGDIELILETCIAQDQQAQLADLAAAV

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:
GO:0003747: Peptide chain release factor 1
GO:0005737: Peptide chain release factor 1
GO:0006412: Peptide chain release factor 1
GO:0006415: Peptide chain release factor 1
GO:0016149: Peptide chain release factor 1

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=284, Percent_Identity=42.9577464788732, Blast_Score=248, Evalue=7e-66,
Organism=Homo sapiens, GI34577120, Length=306, Percent_Identity=36.2745098039216, Blast_Score=224, Evalue=1e-58,
Organism=Homo sapiens, GI166795305, Length=196, Percent_Identity=39.2857142857143, Blast_Score=164, Evalue=1e-40,
Organism=Escherichia coli, GI1787462, Length=357, Percent_Identity=45.0980392156863, Blast_Score=317, Evalue=8e-88,
Organism=Escherichia coli, GI2367172, Length=339, Percent_Identity=35.9882005899705, Blast_Score=205, Evalue=4e-54,
Organism=Caenorhabditis elegans, GI17542784, Length=270, Percent_Identity=37.037037037037, Blast_Score=189, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6321295, Length=294, Percent_Identity=41.4965986394558, Blast_Score=212, Evalue=1e-55,
Organism=Drosophila melanogaster, GI19921226, Length=286, Percent_Identity=40.2097902097902, Blast_Score=214, Evalue=9e-56,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_ACAM1 (B0C424)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001515598.1
- ProteinModelPortal:   B0C424
- SMR:   B0C424
- GeneID:   5680070
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_1247
- HOGENOM:   HBG629764
- OMA:   SEQGGYK
- ProtClustDB:   PRK00591
- BioCyc:   AMAR329726:AM1_1247-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 40511; Mature: 40380

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEAYLLEKLKSVEQTFHELTRRLGDPEVATNPTEFQEVAKARASLEETVNTFEDWKATQ
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QELADAQQMAKDGDEDPELKEMAAMEAAELTEKLDGLETKLKVLLLPRDPNDEKNIMLEI
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCEEEEE
RAGAGGDEASIWAGDLVRIYSKYAESQKWRVKLLSESGGEMGGFKEAILEIQGERVYSKL
ECCCCCCCCHHHHHHHHHHHHHHHCCCHHEEEEECCCCCCCCHHHHHHHHHHHHHHHHHH
KYEAGVHRVQRVPATEASGRVHTSTATVAVMPEVDDVEIEIDPKDIEMSTARSGGAGGQN
HHHHHHHHHHHCCCCCCCCEEEECCEEEEEECCCCCEEEEECCCCCCHHCCCCCCCCCCC
VNKVETAVDLVHKPTGIRIFCTEERSQLQNKERAMQILRAKLYEMELQEQQASVTSMRRS
HHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QVGTGARSEKIRTYNYKDNRATDHRLNQNFPLEKVLVGDIELILETCIAQDQQAQLADLA
HCCCCCCCCCEEEECCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
AAV
HCC
>Mature Secondary Structure 
AEAYLLEKLKSVEQTFHELTRRLGDPEVATNPTEFQEVAKARASLEETVNTFEDWKATQ
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QELADAQQMAKDGDEDPELKEMAAMEAAELTEKLDGLETKLKVLLLPRDPNDEKNIMLEI
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCEEEEE
RAGAGGDEASIWAGDLVRIYSKYAESQKWRVKLLSESGGEMGGFKEAILEIQGERVYSKL
ECCCCCCCCHHHHHHHHHHHHHHHCCCHHEEEEECCCCCCCCHHHHHHHHHHHHHHHHHH
KYEAGVHRVQRVPATEASGRVHTSTATVAVMPEVDDVEIEIDPKDIEMSTARSGGAGGQN
HHHHHHHHHHHCCCCCCCCEEEECCEEEEEECCCCCEEEEECCCCCCHHCCCCCCCCCCC
VNKVETAVDLVHKPTGIRIFCTEERSQLQNKERAMQILRAKLYEMELQEQQASVTSMRRS
HHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QVGTGARSEKIRTYNYKDNRATDHRLNQNFPLEKVLVGDIELILETCIAQDQQAQLADLA
HCCCCCCCCCEEEECCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
AAV
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA