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The map label for this gene is menD

Identifier: 158334289

GI number: 158334289

Start: 1084793

End: 1086553

Strand: Reverse

Name: menD

Synonym: AM1_1108

Alternate gene names: 158334289

Gene position: 1086553-1084793 (Counterclockwise)

Preceding gene: 158334290

Following gene: 158334288

Centisome position: 16.71

GC content: 49.18

Gene sequence:

>1761_bases
ATGACCCTAGATTTTCGCAATGTCAATATGTTGTGGTCATCGATTATTGCAGAAACCCTGAGCCGATTAGGGTTAACAAC
TGCCATCATTTGTCCAGGGTCTCGCTCAGGCCCTCTAGCCGTCGCCTTTGCCCAGCATCCTGACATTGAAGCCATTCCAG
TTTTAGACGAGCGTTCGGCCGCCTTTTTTGGGTTAGGTATTGCTCGTCGTAAGGGTTTACCTGTTGTATTAGTGTGCACC
TCCGGGACCGCAGGGGCCAATTTCTACCCAGCCGTCATTGAAGCTGAAGCCAGCGGAGTCCCCTTACTTTTACTCACGGC
AGATCGACCACCAGAACTGCGACACTGCCAGGCGGGCCAAGCCATTGATCAAGTCAAGCTCTATGGACATTATCCACGGT
GGCAGGTTGAGTTAGCATTACCCAGTACTCATCCAGAGATGCTGGCCTATTTACGCCAAACCTGTGTTTACGCCTGGGAA
CAATCACAAAGGCCAGTTCCTGGCCCGGTCCATCTCAATATTCCGTTTCGCGATCCGCTCGCACCTATCTCCGACTTAGA
AACGGAGAGCCTAGCTGCGACGTTCGATATCGATCAATTCTTTGCTGCTGTTCACCCCTTACCCTCGGTCCAGATCTCCC
TCGACCTTCAGACCACCAACGTCTATTTGGATCAGTGGATGAGTTGCGATCGTGGACTCATTATTGCAGGACCTGCACAA
CCCGATACCCCAGAGCACTATTGTTTAGCCATCGCTCAACTCGCCCACATATTGGGTTGGCCGGTACTTGCAGAAGGGCT
TTCTCCCCTGCGCAATTGGGCAGATCGAAATTCTTATCTGATTTCAACCTACGACCTGATATTGCGAAACCCGACTATTG
CCAGTCAGTTACAGCCTCAGCAGGTCATTAGCATCGGAGCGTTGCCAACCAGTAAAGTCTTGAGAGTGTGGCTGACCCAG
AGTCGTGCTCAAACTTGGGTGGTAGACGTCTCTGATCACAATATGGATCCATTGCATGGTCTCTCTATTCCAGTACGCAT
CTCCGTGGAAGGATTGATCACATCTATCCCTGCAGCTAAGAAGGAACACTGTCAGCCGCTTACAGCGTATGCAAAGTCAT
GGTTAAATGCCAATCATTCAGTGCGAACCCTGATCAATCGGGCAATGCAAAAACAGTCAGAACTTATCGAAAGCAAAATC
GCCTGGTTACTTTCTCAAATCCTGCCGCCTCGCACCCCATTATTTATTGCTAATAGCATGCCGGTACGCGATATCGAATA
TTTTTGGCAGCCAGGAACCCTGCATATTCAGCCTTACTTCAATCGAGGGGCAAACGGCATTGATGGCACCCTCTCTACGG
CCTTGGGGATGTCCCATCGTCAACAAAGTAGCGTCCTGTTGACGGGGGACTTAGCGTTACTTCATGACACCAACGGATTT
TTGTTGCAGGATTATTGGCAAGGCCATTTGACAATTATTGTTGTTAACAATGGAGGAGGCGGCATTTTTCAAATGCTGCC
GATCTCAGCCTTTGACCCCCCATTTGAGCGGTTTTTCCGCACCCCACAACAGGTTCAGCTGAAACATTTGGCAGCAACCT
ATCAGGCCAAGTGGACACTTATTCAGTCCTGGGCACACTTGCAACAAGCCTTAAACCCTTTACCAGAAAGCGGTTTACAT
ATATTGGAAATCTCTTGCGATTGCCAGAATGATACCCAATGGAGGAGAATATTTTTTAAGGAATGCGTGAAAAGTCTCTA
A

Upstream 100 bases:

>100_bases
TGTTATAGGAAGATAAGCTTGTATCAGAAGCGGCTGCAGTCCTCTGCTTTCAACCCAACAGCCTCTTCAGATCAAATTAA
CTGTATATGATGCGAGGACG

Downstream 100 bases:

>100_bases
ATGCTAGGAATTTCTCTAAAAGTTCCATCATTTTGGCTTATAACTTTCACTAGATGCTGTCCATCTCTTATGAACTATTT
TTTAGCTTATAAGATGAAAG

Product: 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-carboxylate synthase

Products: NA

Alternate protein names: SEPHCHC synthase; Menaquinone biosynthesis protein menD

Number of amino acids: Translated: 586; Mature: 585

Protein sequence:

>586_residues
MTLDFRNVNMLWSSIIAETLSRLGLTTAIICPGSRSGPLAVAFAQHPDIEAIPVLDERSAAFFGLGIARRKGLPVVLVCT
SGTAGANFYPAVIEAEASGVPLLLLTADRPPELRHCQAGQAIDQVKLYGHYPRWQVELALPSTHPEMLAYLRQTCVYAWE
QSQRPVPGPVHLNIPFRDPLAPISDLETESLAATFDIDQFFAAVHPLPSVQISLDLQTTNVYLDQWMSCDRGLIIAGPAQ
PDTPEHYCLAIAQLAHILGWPVLAEGLSPLRNWADRNSYLISTYDLILRNPTIASQLQPQQVISIGALPTSKVLRVWLTQ
SRAQTWVVDVSDHNMDPLHGLSIPVRISVEGLITSIPAAKKEHCQPLTAYAKSWLNANHSVRTLINRAMQKQSELIESKI
AWLLSQILPPRTPLFIANSMPVRDIEYFWQPGTLHIQPYFNRGANGIDGTLSTALGMSHRQQSSVLLTGDLALLHDTNGF
LLQDYWQGHLTIIVVNNGGGGIFQMLPISAFDPPFERFFRTPQQVQLKHLAATYQAKWTLIQSWAHLQQALNPLPESGLH
ILEISCDCQNDTQWRRIFFKECVKSL

Sequences:

>Translated_586_residues
MTLDFRNVNMLWSSIIAETLSRLGLTTAIICPGSRSGPLAVAFAQHPDIEAIPVLDERSAAFFGLGIARRKGLPVVLVCT
SGTAGANFYPAVIEAEASGVPLLLLTADRPPELRHCQAGQAIDQVKLYGHYPRWQVELALPSTHPEMLAYLRQTCVYAWE
QSQRPVPGPVHLNIPFRDPLAPISDLETESLAATFDIDQFFAAVHPLPSVQISLDLQTTNVYLDQWMSCDRGLIIAGPAQ
PDTPEHYCLAIAQLAHILGWPVLAEGLSPLRNWADRNSYLISTYDLILRNPTIASQLQPQQVISIGALPTSKVLRVWLTQ
SRAQTWVVDVSDHNMDPLHGLSIPVRISVEGLITSIPAAKKEHCQPLTAYAKSWLNANHSVRTLINRAMQKQSELIESKI
AWLLSQILPPRTPLFIANSMPVRDIEYFWQPGTLHIQPYFNRGANGIDGTLSTALGMSHRQQSSVLLTGDLALLHDTNGF
LLQDYWQGHLTIIVVNNGGGGIFQMLPISAFDPPFERFFRTPQQVQLKHLAATYQAKWTLIQSWAHLQQALNPLPESGLH
ILEISCDCQNDTQWRRIFFKECVKSL
>Mature_585_residues
TLDFRNVNMLWSSIIAETLSRLGLTTAIICPGSRSGPLAVAFAQHPDIEAIPVLDERSAAFFGLGIARRKGLPVVLVCTS
GTAGANFYPAVIEAEASGVPLLLLTADRPPELRHCQAGQAIDQVKLYGHYPRWQVELALPSTHPEMLAYLRQTCVYAWEQ
SQRPVPGPVHLNIPFRDPLAPISDLETESLAATFDIDQFFAAVHPLPSVQISLDLQTTNVYLDQWMSCDRGLIIAGPAQP
DTPEHYCLAIAQLAHILGWPVLAEGLSPLRNWADRNSYLISTYDLILRNPTIASQLQPQQVISIGALPTSKVLRVWLTQS
RAQTWVVDVSDHNMDPLHGLSIPVRISVEGLITSIPAAKKEHCQPLTAYAKSWLNANHSVRTLINRAMQKQSELIESKIA
WLLSQILPPRTPLFIANSMPVRDIEYFWQPGTLHIQPYFNRGANGIDGTLSTALGMSHRQQSSVLLTGDLALLHDTNGFL
LQDYWQGHLTIIVVNNGGGGIFQMLPISAFDPPFERFFRTPQQVQLKHLAATYQAKWTLIQSWAHLQQALNPLPESGLHI
LEISCDCQNDTQWRRIFFKECVKSL

Specific function: Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-ca

COG id: COG1165

COG function: function code H; 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family. MenD subfamily

Homologues:

Organism=Escherichia coli, GI1788599, Length=548, Percent_Identity=31.5693430656934, Blast_Score=218, Evalue=6e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MEND_ACAM1 (B0C1Y1)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001515461.1
- ProteinModelPortal:   B0C1Y1
- GeneID:   5679933
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_1108
- HOGENOM:   HBG634405
- OMA:   FYHDLNG
- ProtClustDB:   PRK07449
- BioCyc:   AMAR329726:AM1_1108-MONOMER
- HAMAP:   MF_01659
- InterPro:   IPR004433
- InterPro:   IPR012001
- TIGRFAMs:   TIGR00173

Pfam domain/function: PF02776 TPP_enzyme_N

EC number: =2.2.1.9

Molecular weight: Translated: 65166; Mature: 65035

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLDFRNVNMLWSSIIAETLSRLGLTTAIICPGSRSGPLAVAFAQHPDIEAIPVLDERSA
CCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEECCCCCCEEECCCCCCC
AFFGLGIARRKGLPVVLVCTSGTAGANFYPAVIEAEASGVPLLLLTADRPPELRHCQAGQ
EEEECCHHHCCCCCEEEEECCCCCCCCCCCEEEEECCCCCCEEEEECCCCCCHHHCCCCC
AIDQVKLYGHYPRWQVELALPSTHPEMLAYLRQTCVYAWEQSQRPVPGPVHLNIPFRDPL
CHHHEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCC
APISDLETESLAATFDIDQFFAAVHPLPSVQISLDLQTTNVYLDQWMSCDRGLIIAGPAQ
CCCCCCCHHHEEEEECHHHHHHHHCCCCCEEEEEEEEEHHHHHHHHHCCCCCEEEECCCC
PDTPEHYCLAIAQLAHILGWPVLAEGLSPLRNWADRNSYLISTYDLILRNPTIASQLQPQ
CCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCHHHHCCHH
QVISIGALPTSKVLRVWLTQSRAQTWVVDVSDHNMDPLHGLSIPVRISVEGLITSIPAAK
HHHEECCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCEEEEEEECHHHHCCCCCH
KEHCQPLTAYAKSWLNANHSVRTLINRAMQKQSELIESKIAWLLSQILPPRTPLFIANSM
HHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCC
PVRDIEYFWQPGTLHIQPYFNRGANGIDGTLSTALGMSHRQQSSVLLTGDLALLHDTNGF
CCCHHHHEECCCEEEEEECCCCCCCCCCCHHHHHHCCCCCCCCCEEEEECEEEEECCCCC
LLQDYWQGHLTIIVVNNGGGGIFQMLPISAFDPPFERFFRTPQQVQLKHLAATYQAKWTL
EEEEECCCEEEEEEEECCCCCEEEEECCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHH
IQSWAHLQQALNPLPESGLHILEISCDCQNDTQWRRIFFKECVKSL
HHHHHHHHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TLDFRNVNMLWSSIIAETLSRLGLTTAIICPGSRSGPLAVAFAQHPDIEAIPVLDERSA
CCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEECCCCCCEEECCCCCCC
AFFGLGIARRKGLPVVLVCTSGTAGANFYPAVIEAEASGVPLLLLTADRPPELRHCQAGQ
EEEECCHHHCCCCCEEEEECCCCCCCCCCCEEEEECCCCCCEEEEECCCCCCHHHCCCCC
AIDQVKLYGHYPRWQVELALPSTHPEMLAYLRQTCVYAWEQSQRPVPGPVHLNIPFRDPL
CHHHEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCC
APISDLETESLAATFDIDQFFAAVHPLPSVQISLDLQTTNVYLDQWMSCDRGLIIAGPAQ
CCCCCCCHHHEEEEECHHHHHHHHCCCCCEEEEEEEEEHHHHHHHHHCCCCCEEEECCCC
PDTPEHYCLAIAQLAHILGWPVLAEGLSPLRNWADRNSYLISTYDLILRNPTIASQLQPQ
CCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCHHHHCCHH
QVISIGALPTSKVLRVWLTQSRAQTWVVDVSDHNMDPLHGLSIPVRISVEGLITSIPAAK
HHHEECCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCEEEEEEECHHHHCCCCCH
KEHCQPLTAYAKSWLNANHSVRTLINRAMQKQSELIESKIAWLLSQILPPRTPLFIANSM
HHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCC
PVRDIEYFWQPGTLHIQPYFNRGANGIDGTLSTALGMSHRQQSSVLLTGDLALLHDTNGF
CCCHHHHEECCCEEEEEECCCCCCCCCCCHHHHHHCCCCCCCCCEEEEECEEEEECCCCC
LLQDYWQGHLTIIVVNNGGGGIFQMLPISAFDPPFERFFRTPQQVQLKHLAATYQAKWTL
EEEEECCCEEEEEEEECCCCCEEEEECCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHH
IQSWAHLQQALNPLPESGLHILEISCDCQNDTQWRRIFFKECVKSL
HHHHHHHHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA