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The map label for this gene is 158334040

Identifier: 158334040

GI number: 158334040

Start: 832939

End: 834093

Strand: Reverse

Name: 158334040

Synonym: AM1_0854

Alternate gene names: NA

Gene position: 834093-832939 (Counterclockwise)

Preceding gene: 158334041

Following gene: 158334038

Centisome position: 12.82

GC content: 51.34

Gene sequence:

>1155_bases
ATGGAAAATCCCTTGTTGCTGATGATTCCGGGTCCCACTCCAGTGCCTGAACCCGTATTGTTTGCTTCGGCTAGACAGCC
CATCAGCCATCGTAGCCAGGATTTTCGGGATTTGATGGCTGATATTACGGTCCATTTAAAATGGCTTCACCAAACCCAAA
ATGAGGTGTTGATACTAGCCAGCAGCGGTACCGGAGCCATGGAGGCTGGCATCATTAATTTTCTGAGCCCAGGTGATCGG
GTATTGGTGGGATGCAATGGTAAGTTTGGCGATCGCTGGACGCTGGTTTGTGAACAATTTGGCCTGATAACTGAACGGAT
TACGGCCCCTTGGGGAAAGGCTTTGGATCCAAAACGGTTTCGGCAGCATTTAGAGGCGGATCTGGATAAGCAAATCAAAG
CAGTGATTGTCACCCACAGCGAGACCTCCACCGGGGTTCTGAACGATCTTGAAACCATTAGCCGTCATATCAAAGCCCAT
GGTCAAGCTCTTAGCATTATCGATGCAGTCACCAGTTTGGGAATTTGTCCCGTTCCCGTCGACGAATGGGGTCTGGATGT
GGTGGTCTCTGCATCCCAAAAAGGGTATCGAATGCCGCCGGGACTGGGGTTTGTTTGCGTCAGCCCTAAAGCGTGGCAGG
CTTATGAAACGGCTCGGTTCCCTCGGTTTTATTTTGACCTAGGGAAATGCCGCACGGATGCAGCGACGAATACGACCCCG
TTTACCCCACCGGTCAATTTATTCTTTGCTTTGCAGATGAGTTTAACCCTGATGAAGGCAGAAGGGCTTTCCGCTATGTT
TGCCCATCAGCAGCGGTTGATGCAGGCAACCCGAGCCGCTCTGCACGCCCTGGACCTGCCTTTATTTGTACCGGATCATT
ATGTAGCTAGCCCCGCTGTTACTGCCATTGCCCCTGATCAGGTGGCGCCAGAAACCCTTCGATTCAAACTACAGCAGCAA
TACGGGGTGGTGATTGCTGCAGGGCAAGATCATATGCGAGGTAAGCTTGTCCGCATCGGCCACTTGGGTTATGTTTGCGA
TCGCAGTATCGTTACTACCATTGCCGCTATAGAAGCAGCCTTAAAAGATTTAGACTATCCATTTCAGGTTGGGGCAGGTG
TGGAGGCTGCTCACCGCATCTTCAAAGGGAGTTAG

Upstream 100 bases:

>100_bases
AGAACAACCGGCTTGCTGTTTGCTGCGTTGGCGTCACCATCCTCAGACCCAAACCCTGAAGTGGCAGCGCCTGTTTCCCT
CTGAAGATGCTTAATGCCTT

Downstream 100 bases:

>100_bases
GATTTGCCTAACCCTATGTTTCAAATCCCTTAAATTTGCAAATTATGTTGTTTCCAGCCAGTCATAAATTTGATTGAGTT
GATCCAAAGTAATCAAACCA

Product: soluble hydrogenase 42 kDa subunit

Products: NA

Alternate protein names: Tritium exchange subunit [H]

Number of amino acids: Translated: 384; Mature: 384

Protein sequence:

>384_residues
MENPLLLMIPGPTPVPEPVLFASARQPISHRSQDFRDLMADITVHLKWLHQTQNEVLILASSGTGAMEAGIINFLSPGDR
VLVGCNGKFGDRWTLVCEQFGLITERITAPWGKALDPKRFRQHLEADLDKQIKAVIVTHSETSTGVLNDLETISRHIKAH
GQALSIIDAVTSLGICPVPVDEWGLDVVVSASQKGYRMPPGLGFVCVSPKAWQAYETARFPRFYFDLGKCRTDAATNTTP
FTPPVNLFFALQMSLTLMKAEGLSAMFAHQQRLMQATRAALHALDLPLFVPDHYVASPAVTAIAPDQVAPETLRFKLQQQ
YGVVIAAGQDHMRGKLVRIGHLGYVCDRSIVTTIAAIEAALKDLDYPFQVGAGVEAAHRIFKGS

Sequences:

>Translated_384_residues
MENPLLLMIPGPTPVPEPVLFASARQPISHRSQDFRDLMADITVHLKWLHQTQNEVLILASSGTGAMEAGIINFLSPGDR
VLVGCNGKFGDRWTLVCEQFGLITERITAPWGKALDPKRFRQHLEADLDKQIKAVIVTHSETSTGVLNDLETISRHIKAH
GQALSIIDAVTSLGICPVPVDEWGLDVVVSASQKGYRMPPGLGFVCVSPKAWQAYETARFPRFYFDLGKCRTDAATNTTP
FTPPVNLFFALQMSLTLMKAEGLSAMFAHQQRLMQATRAALHALDLPLFVPDHYVASPAVTAIAPDQVAPETLRFKLQQQ
YGVVIAAGQDHMRGKLVRIGHLGYVCDRSIVTTIAAIEAALKDLDYPFQVGAGVEAAHRIFKGS
>Mature_384_residues
MENPLLLMIPGPTPVPEPVLFASARQPISHRSQDFRDLMADITVHLKWLHQTQNEVLILASSGTGAMEAGIINFLSPGDR
VLVGCNGKFGDRWTLVCEQFGLITERITAPWGKALDPKRFRQHLEADLDKQIKAVIVTHSETSTGVLNDLETISRHIKAH
GQALSIIDAVTSLGICPVPVDEWGLDVVVSASQKGYRMPPGLGFVCVSPKAWQAYETARFPRFYFDLGKCRTDAATNTTP
FTPPVNLFFALQMSLTLMKAEGLSAMFAHQQRLMQATRAALHALDLPLFVPDHYVASPAVTAIAPDQVAPETLRFKLQQQ
YGVVIAAGQDHMRGKLVRIGHLGYVCDRSIVTTIAAIEAALKDLDYPFQVGAGVEAAHRIFKGS

Specific function: Soluble hydrogenase catalyzes both production and consumption of hydrogen from suitable artificial electron donors or acceptors. This subunit catalyzes the tritium-exchange activity [H]

COG id: COG0075

COG function: function code E; Serine-pyruvate aminotransferase/archaeal aspartate aminotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI4557289, Length=368, Percent_Identity=30.9782608695652, Blast_Score=144, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17536281, Length=363, Percent_Identity=30.3030303030303, Blast_Score=147, Evalue=7e-36,
Organism=Saccharomyces cerevisiae, GI6321079, Length=366, Percent_Identity=26.5027322404372, Blast_Score=97, Evalue=3e-21,
Organism=Drosophila melanogaster, GI17530823, Length=351, Percent_Identity=26.2108262108262, Blast_Score=103, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: 2.6.1.-

Molecular weight: Translated: 42056; Mature: 42056

Theoretical pI: Translated: 7.39; Mature: 7.39

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENPLLLMIPGPTPVPEPVLFASARQPISHRSQDFRDLMADITVHLKWLHQTQNEVLILA
CCCCEEEEECCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
SSGTGAMEAGIINFLSPGDRVLVGCNGKFGDRWTLVCEQFGLITERITAPWGKALDPKRF
CCCCCCHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
RQHLEADLDKQIKAVIVTHSETSTGVLNDLETISRHIKAHGQALSIIDAVTSLGICPVPV
HHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCC
DEWGLDVVVSASQKGYRMPPGLGFVCVSPKAWQAYETARFPRFYFDLGKCRTDAATNTTP
CCCCCEEEEECCCCCCCCCCCCEEEEECCCHHHHHHHHCCCHHHHHHHHHCCCCCCCCCC
FTPPVNLFFALQMSLTLMKAEGLSAMFAHQQRLMQATRAALHALDLPLFVPDHYVASPAV
CCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCE
TAIAPDQVAPETLRFKLQQQYGVVIAAGQDHMRGKLVRIGHLGYVCDRSIVTTIAAIEAA
EEECCCCCCCHHHHHHHHHCCCEEEECCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHH
LKDLDYPFQVGAGVEAAHRIFKGS
HHHCCCCEECCCCHHHHHHHHCCC
>Mature Secondary Structure
MENPLLLMIPGPTPVPEPVLFASARQPISHRSQDFRDLMADITVHLKWLHQTQNEVLILA
CCCCEEEEECCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
SSGTGAMEAGIINFLSPGDRVLVGCNGKFGDRWTLVCEQFGLITERITAPWGKALDPKRF
CCCCCCHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
RQHLEADLDKQIKAVIVTHSETSTGVLNDLETISRHIKAHGQALSIIDAVTSLGICPVPV
HHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCC
DEWGLDVVVSASQKGYRMPPGLGFVCVSPKAWQAYETARFPRFYFDLGKCRTDAATNTTP
CCCCCEEEEECCCCCCCCCCCCEEEEECCCHHHHHHHHCCCHHHHHHHHHCCCCCCCCCC
FTPPVNLFFALQMSLTLMKAEGLSAMFAHQQRLMQATRAALHALDLPLFVPDHYVASPAV
CCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCE
TAIAPDQVAPETLRFKLQQQYGVVIAAGQDHMRGKLVRIGHLGYVCDRSIVTTIAAIEAA
EEECCCCCCCHHHHHHHHHCCCEEEECCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHH
LKDLDYPFQVGAGVEAAHRIFKGS
HHHCCCCEECCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2513553 [H]