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The map label for this gene is walK [H]

Identifier: 158334015

GI number: 158334015

Start: 807889

End: 810033

Strand: Reverse

Name: walK [H]

Synonym: AM1_0829

Alternate gene names: 158334015

Gene position: 810033-807889 (Counterclockwise)

Preceding gene: 158334016

Following gene: 158334014

Centisome position: 12.45

GC content: 49.23

Gene sequence:

>2145_bases
ATGAGTCTGCCGTTATCCGCAATGAATTTAGAGAAGCACAACCAACGGATGCAATTGTTAGCGGATGTTACCTACAAAAT
TAGGCAATCCCTGGATCTCCACGAAATTCTGCAGACCACTGTTACTGAGGTGCGCCAGCTCCTAGATGCTGATCGCGTTG
TCATGTTCCAAATTCATCCTGATGGGGCAGGAACTGTGGTGCAAGAAGCAGTGTTGCCCGGTTGGTCAAAATCCATTAAC
CAAAATATTGTCGATACCTGTTTTGGTACCAGCTTTATCGATTCCTATCGACAGGGGCGCGTTTCGGCCATCAGCGATAT
CGAAACAGCGCCGATTACCCCTTGCCATGCTGATTTACTACGGCAATTTGAAGTAAAGGCCAATCTGGTTATCCCGATCA
TTAGTCAGGGACAGCTGTGGGGGTTGCTGATCGCTCACCAATGTTCAGCCCCAAGGGAATGGCAAGCCTTTGAAGTGGAC
TTGCTCAAACAACTTGCCGATCAAGCGGGAATTGCCCTAGCTCAGGCCAAACTCTTGGCCGATTTGAATCAAGCCCATCA
ACAGCTCAGATTTCATGTGGAGAATTCTCCCCTAGCGGTGATCGAATGGGATCATGCTTGCCGCATTCAGCGCTGGTCGG
CTCAGGCTGAACGAATTTTTGGTTGGTCGGGAGAGGAAGTCATCGGCCGAGGGCCAGATCACTGGTTTATTCATACCGAC
GATCTCCATCTCTTTCGAGAACATGTGCTGAACCTGACTGGACACCCCTTTCCCAATTACGCCAATCCAGATATGGAGCG
GTTGACCCGCAACTATACGAAAGCAGGCGATCTGATTGATTGCGAGTGGTACAGCTCAGCCCTGTTTGATGCAGAGGGCA
ACTTGGTGTCGATCCTCTCCATGGCACAGGATGTAAGCGCTCGCCAGCAGGCCGAAGCTGCTTTACAACAGCTCAATCGA
GAGTTAGAGATGCGGGTTGAGCAACGGACAGCCGCCTTGCAAGAAAGCGAGCAGCGATTTCGATCTTTATTTGAAGCTGC
CCCCGATTTTATCTATGTTTTAGACACCAAAGGGGTGATTCAGCATGTTAACCCGGTGGTGATTGAACGATCAGGCTACC
GTAAATCCGAACTAGAAGGTCATCCTTTCATTAATCTCTTTGCCCCTAAATCTCAGGTGCTCTGCCAGCAGCAATTTGGA
GATTTACTTGCCCAGGGGAGCCATCGGCAAGAGCTGGAGTTTATGGGCAAAAATGGCAAGATTTTGACCGTGGATTGCTC
TTTCAAAGTGGTTAAAGACCCCCTGGGAAAAACATCGATTTTGGTGGTGCAACGGGACATTAGCGATCGCAAAGCCTTAG
AACGGATCAAAGACGAATTTATTTCCGTGGTGAGCCACGAGTTACGAACGCCTTTGACTTCAATTCACGGATCGATCAAG
CTCCTAGCCACAGGCCAACTCGGGGATTTATCGGATGATGGCCAACAAATGCTCGATATTGCCGATCAAAATACCGATCG
TCTCGTTCGTCTAGTCAGTGATGTTCTGGACTTACAACGGATCGAATCGGGGCAAGCCAAGATTAGTAAGCACCTCTGCG
ATGCGGCAGACTTAATGACCCATGCTACAGACGCGATGCGGAGTATGGCCTACCACCATCAGGTCACCTTAAAAAGCACG
CCGCTCTTAACCATGGTTTGGGCCAATCCAGATTACATGGTGCAAACCCTAACTAATTTGATCAGCAATGCCATTAAGTT
TTCCCAGCCCGGTGGAACGGTTGAGTTAGCAGCTGAACTTCGGATTGAAGAGCGACGAGCCAAAGATAGCTCCTCTCCAC
CAGAGAGTCATGACACCCCCTTTCCCATCACGATTAAACATACTGAACTGTGGTTTCAAGTGATTGACCAGGGCCAAGGC
ATTCCGGCTGATAAACTGGAAAGCGTTTTTGAGCGCTTTCAACAAGTCGATGCTTCAGATGCTCGGGAAAAAGGTGGTAC
TGGTCTGGGCTTAGCCATCTGCCGCAAAATTGTAGAACAACATGGTGGCAAAATCTGGGCCGAAAGTGTTTTGGGCCAAG
GCAGCTGCTTTTTTGTGGCCTTACCCATGTCCTGTTGGTTAGTGGAAAAAGCTGAGGTCAGTTAA

Upstream 100 bases:

>100_bases
TAGATGCCCAGACCCCTCGTTTGACCTTTATGATGGGGGCAGATTCTTATTTCTAGACTGAAACAGTTGCTATCCGAATA
CAGATCACAGCCTTTTATCC

Downstream 100 bases:

>100_bases
TCGTTGGTTTCCATCCCCTATACCTAGACCCAATCTATGGCAGAAAAACGCATCCTGATTATTGATGATGAGCAAGCGAT
TCAAGCTGTAGCTAGCCTCA

Product: two-component sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 714; Mature: 713

Protein sequence:

>714_residues
MSLPLSAMNLEKHNQRMQLLADVTYKIRQSLDLHEILQTTVTEVRQLLDADRVVMFQIHPDGAGTVVQEAVLPGWSKSIN
QNIVDTCFGTSFIDSYRQGRVSAISDIETAPITPCHADLLRQFEVKANLVIPIISQGQLWGLLIAHQCSAPREWQAFEVD
LLKQLADQAGIALAQAKLLADLNQAHQQLRFHVENSPLAVIEWDHACRIQRWSAQAERIFGWSGEEVIGRGPDHWFIHTD
DLHLFREHVLNLTGHPFPNYANPDMERLTRNYTKAGDLIDCEWYSSALFDAEGNLVSILSMAQDVSARQQAEAALQQLNR
ELEMRVEQRTAALQESEQRFRSLFEAAPDFIYVLDTKGVIQHVNPVVIERSGYRKSELEGHPFINLFAPKSQVLCQQQFG
DLLAQGSHRQELEFMGKNGKILTVDCSFKVVKDPLGKTSILVVQRDISDRKALERIKDEFISVVSHELRTPLTSIHGSIK
LLATGQLGDLSDDGQQMLDIADQNTDRLVRLVSDVLDLQRIESGQAKISKHLCDAADLMTHATDAMRSMAYHHQVTLKST
PLLTMVWANPDYMVQTLTNLISNAIKFSQPGGTVELAAELRIEERRAKDSSSPPESHDTPFPITIKHTELWFQVIDQGQG
IPADKLESVFERFQQVDASDAREKGGTGLGLAICRKIVEQHGGKIWAESVLGQGSCFFVALPMSCWLVEKAEVS

Sequences:

>Translated_714_residues
MSLPLSAMNLEKHNQRMQLLADVTYKIRQSLDLHEILQTTVTEVRQLLDADRVVMFQIHPDGAGTVVQEAVLPGWSKSIN
QNIVDTCFGTSFIDSYRQGRVSAISDIETAPITPCHADLLRQFEVKANLVIPIISQGQLWGLLIAHQCSAPREWQAFEVD
LLKQLADQAGIALAQAKLLADLNQAHQQLRFHVENSPLAVIEWDHACRIQRWSAQAERIFGWSGEEVIGRGPDHWFIHTD
DLHLFREHVLNLTGHPFPNYANPDMERLTRNYTKAGDLIDCEWYSSALFDAEGNLVSILSMAQDVSARQQAEAALQQLNR
ELEMRVEQRTAALQESEQRFRSLFEAAPDFIYVLDTKGVIQHVNPVVIERSGYRKSELEGHPFINLFAPKSQVLCQQQFG
DLLAQGSHRQELEFMGKNGKILTVDCSFKVVKDPLGKTSILVVQRDISDRKALERIKDEFISVVSHELRTPLTSIHGSIK
LLATGQLGDLSDDGQQMLDIADQNTDRLVRLVSDVLDLQRIESGQAKISKHLCDAADLMTHATDAMRSMAYHHQVTLKST
PLLTMVWANPDYMVQTLTNLISNAIKFSQPGGTVELAAELRIEERRAKDSSSPPESHDTPFPITIKHTELWFQVIDQGQG
IPADKLESVFERFQQVDASDAREKGGTGLGLAICRKIVEQHGGKIWAESVLGQGSCFFVALPMSCWLVEKAEVS
>Mature_713_residues
SLPLSAMNLEKHNQRMQLLADVTYKIRQSLDLHEILQTTVTEVRQLLDADRVVMFQIHPDGAGTVVQEAVLPGWSKSINQ
NIVDTCFGTSFIDSYRQGRVSAISDIETAPITPCHADLLRQFEVKANLVIPIISQGQLWGLLIAHQCSAPREWQAFEVDL
LKQLADQAGIALAQAKLLADLNQAHQQLRFHVENSPLAVIEWDHACRIQRWSAQAERIFGWSGEEVIGRGPDHWFIHTDD
LHLFREHVLNLTGHPFPNYANPDMERLTRNYTKAGDLIDCEWYSSALFDAEGNLVSILSMAQDVSARQQAEAALQQLNRE
LEMRVEQRTAALQESEQRFRSLFEAAPDFIYVLDTKGVIQHVNPVVIERSGYRKSELEGHPFINLFAPKSQVLCQQQFGD
LLAQGSHRQELEFMGKNGKILTVDCSFKVVKDPLGKTSILVVQRDISDRKALERIKDEFISVVSHELRTPLTSIHGSIKL
LATGQLGDLSDDGQQMLDIADQNTDRLVRLVSDVLDLQRIESGQAKISKHLCDAADLMTHATDAMRSMAYHHQVTLKSTP
LLTMVWANPDYMVQTLTNLISNAIKFSQPGGTVELAAELRIEERRAKDSSSPPESHDTPFPITIKHTELWFQVIDQGQGI
PADKLESVFERFQQVDASDAREKGGTGLGLAICRKIVEQHGGKIWAESVLGQGSCFFVALPMSCWLVEKAEVS

Specific function: Member of the two-component regulatory system walK/walR that regulates genes involved in autolysis, biofilm formation and cell wall metabolism. WalK functions as a sensor protein kinase which is autophosphorylated at a histidine residue and transfers its

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI145693157, Length=414, Percent_Identity=28.9855072463768, Blast_Score=122, Evalue=9e-29,
Organism=Escherichia coli, GI48994928, Length=423, Percent_Identity=25.2955082742317, Blast_Score=119, Evalue=1e-27,
Organism=Escherichia coli, GI1786600, Length=382, Percent_Identity=24.869109947644, Blast_Score=113, Evalue=4e-26,
Organism=Escherichia coli, GI1789149, Length=284, Percent_Identity=28.5211267605634, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI1788713, Length=274, Percent_Identity=25.9124087591241, Blast_Score=105, Evalue=1e-23,
Organism=Escherichia coli, GI1786912, Length=249, Percent_Identity=28.5140562248996, Blast_Score=99, Evalue=1e-21,
Organism=Escherichia coli, GI1788393, Length=251, Percent_Identity=27.0916334661355, Blast_Score=97, Evalue=5e-21,
Organism=Escherichia coli, GI87082128, Length=256, Percent_Identity=26.953125, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI1790346, Length=306, Percent_Identity=26.4705882352941, Blast_Score=84, Evalue=4e-17,
Organism=Escherichia coli, GI1788549, Length=425, Percent_Identity=24, Blast_Score=84, Evalue=4e-17,
Organism=Escherichia coli, GI1786783, Length=267, Percent_Identity=26.5917602996255, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI1790861, Length=237, Percent_Identity=27.0042194092827, Blast_Score=79, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 80050; Mature: 79919

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50109 HIS_KIN ; PS50046 PHYTOCHROME_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLPLSAMNLEKHNQRMQLLADVTYKIRQSLDLHEILQTTVTEVRQLLDADRVVMFQIHP
CCCCCCHHCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEEECC
DGAGTVVQEAVLPGWSKSINQNIVDTCFGTSFIDSYRQGRVSAISDIETAPITPCHADLL
CCCCHHHHHHHCCCCHHHHHHHHHHHHHCHHHHHHHHCCCHHHHHCCCCCCCCCHHHHHH
RQFEVKANLVIPIISQGQLWGLLIAHQCSAPREWQAFEVDLLKQLADQAGIALAQAKLLA
HHHHHCCCEEEEEECCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHH
DLNQAHQQLRFHVENSPLAVIEWDHACRIQRWSAQAERIFGWSGEEVIGRGPDHWFIHTD
HHHHHHHHHEEEECCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCHHHCCCCCEEEEECC
DLHLFREHVLNLTGHPFPNYANPDMERLTRNYTKAGDLIDCEWYSSALFDAEGNLVSILS
HHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEHHHHHHHCCCCCCHHHHHH
MAQDVSARQQAEAALQQLNRELEMRVEQRTAALQESEQRFRSLFEAAPDFIYVLDTKGVI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHH
QHVNPVVIERSGYRKSELEGHPFINLFAPKSQVLCQQQFGDLLAQGSHRQELEFMGKNGK
HHCCCEEEECCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCC
ILTVDCSFKVVKDPLGKTSILVVQRDISDRKALERIKDEFISVVSHELRTPLTSIHGSIK
EEEEECCHHEECCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCEE
LLATGQLGDLSDDGQQMLDIADQNTDRLVRLVSDVLDLQRIESGQAKISKHLCDAADLMT
EEEECCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
HATDAMRSMAYHHQVTLKSTPLLTMVWANPDYMVQTLTNLISNAIKFSQPGGTVELAAEL
HHHHHHHHHHHHHEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCEEEHHHH
RIEERRAKDSSSPPESHDTPFPITIKHTELWFQVIDQGQGIPADKLESVFERFQQVDASD
HHHHHHCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHH
AREKGGTGLGLAICRKIVEQHGGKIWAESVLGQGSCFFVALPMSCWLVEKAEVS
HHHCCCCCHHHHHHHHHHHHCCCEEEHHHHCCCCCEEEEEECCHHEEEECCCCC
>Mature Secondary Structure 
SLPLSAMNLEKHNQRMQLLADVTYKIRQSLDLHEILQTTVTEVRQLLDADRVVMFQIHP
CCCCCHHCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEEECC
DGAGTVVQEAVLPGWSKSINQNIVDTCFGTSFIDSYRQGRVSAISDIETAPITPCHADLL
CCCCHHHHHHHCCCCHHHHHHHHHHHHHCHHHHHHHHCCCHHHHHCCCCCCCCCHHHHHH
RQFEVKANLVIPIISQGQLWGLLIAHQCSAPREWQAFEVDLLKQLADQAGIALAQAKLLA
HHHHHCCCEEEEEECCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHH
DLNQAHQQLRFHVENSPLAVIEWDHACRIQRWSAQAERIFGWSGEEVIGRGPDHWFIHTD
HHHHHHHHHEEEECCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCHHHCCCCCEEEEECC
DLHLFREHVLNLTGHPFPNYANPDMERLTRNYTKAGDLIDCEWYSSALFDAEGNLVSILS
HHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEHHHHHHHCCCCCCHHHHHH
MAQDVSARQQAEAALQQLNRELEMRVEQRTAALQESEQRFRSLFEAAPDFIYVLDTKGVI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHH
QHVNPVVIERSGYRKSELEGHPFINLFAPKSQVLCQQQFGDLLAQGSHRQELEFMGKNGK
HHCCCEEEECCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCC
ILTVDCSFKVVKDPLGKTSILVVQRDISDRKALERIKDEFISVVSHELRTPLTSIHGSIK
EEEEECCHHEECCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCEE
LLATGQLGDLSDDGQQMLDIADQNTDRLVRLVSDVLDLQRIESGQAKISKHLCDAADLMT
EEEECCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
HATDAMRSMAYHHQVTLKSTPLLTMVWANPDYMVQTLTNLISNAIKFSQPGGTVELAAEL
HHHHHHHHHHHHHEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCEEEHHHH
RIEERRAKDSSSPPESHDTPFPITIKHTELWFQVIDQGQGIPADKLESVFERFQQVDASD
HHHHHHCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHH
AREKGGTGLGLAICRKIVEQHGGKIWAESVLGQGSCFFVALPMSCWLVEKAEVS
HHHCCCCCHHHHHHHHHHHHCCCEEEHHHHCCCCCEEEEEECCHHEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA