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The map label for this gene is wcaL [H]

Identifier: 158333987

GI number: 158333987

Start: 777464

End: 778669

Strand: Reverse

Name: wcaL [H]

Synonym: AM1_0800

Alternate gene names: 158333987

Gene position: 778669-777464 (Counterclockwise)

Preceding gene: 158333989

Following gene: 158333983

Centisome position: 11.97

GC content: 47.01

Gene sequence:

>1206_bases
ATGACTACGGTTGCTTATCTGATTAATCAATACCCCAAGGTCAGTCATAGCTTTATTCGCCGTGAAATTTTGGCCTTAGA
AGCACAGGGGTTGAATATTCGCCGTTATTCCATTCGTCCCTGTCCTGATCAGCTCATCGATCCTGATGATGTGGCAGAGC
TAGATAAGACTCAAGTCCTGCTGTCCGTTGGCGTCTTAGGACTTGTTTTAGCATTGATTTGGGTCATGTTTAGCCGCCCC
CTTGCCTTTGTTAAAGCTCTGGGGTTAACCCTTAAGATTGGTTGGCATTCTGATCGTGGCTTGTGGCGGCACTTTATCTA
CTTGGCAGAAGCAAGTGTTTTCCTGTTTTGGCTAGGCGTTGCAGGGGTTGACCATGTTCATGCCCATTTCGGCACGAACC
CTGCTGCCGTCGCCATGTTGTGTCATGCCTTGGGTGGACCTTCCTATAGCTTTACGGTGCACGGGCCCGACGAATTTGAT
AAACCCCAGATTCTTCACTTAGCGACCAAGGTTGCCCGTGCTGATTTTGTGGTGGCAGTTAGCTCCTTTGGCCAAAGCCA
ACTCTATCGTTGGAGTAACCCTCAACATTGGTCTAAAATCCACATTGTTCGCTGTGGATTAGATCAAATATTTCTGCATC
AACCCCCTACCGCGTTGCCAGAAACGCCTCATATCGTTTGTATTGGCCGTCTTTGTCCTGCAAAGGGACAACTTTTGTTG
GTTGAGGCTGTTTCCCAGTTGATCCAAGAGGGAGTCGAAATTAAGTTGACTTTGGTCGGAGATGGTGAATCCCGTGCTGC
CATAGAATCCGCCATTGCCGCAGTACATTTAGAAGAGGTAGTTACCATCACAGGATGGGTGAGCAGTATCCATATTCGGA
ACTATTTACTAGGGGCCAAAGTTAAAGTTTTACCGAGCTTTGCAGAAGGCTTACCCGTCGTTTTAATGGAAGCTTTAGCC
CTAAGACGTCCTGTAATTTCCACCTATATTGCAGGTATCCCTGAATTAGTAGAACCTAGCCATGGCTGGTTGATTCCAGC
GGGGTCAGTTACAGCTTTAAAGGATGCGATTCAAGTTAGTTTAGCCACCAGTACAGTCCAACTCCAAGAAATGGGACAAG
TTGGGTCTGAACGGGTACTTGCGGATCACAATATAGAAACTGAAGTAGAGAAACTCTCGCAATTATTTGCAGCAAATCGG
CTCTAG

Upstream 100 bases:

>100_bases
GGTGCTGGAGCTGATGTTACTTCAGACCGTTCATCCCCATACGCAGCGCAAGAAAAATTGCTACCCAGGGTAGTCTAGGA
CTACCATTGCCGACCCAATT

Downstream 100 bases:

>100_bases
ATTTTAAAACCCTTAGAGGTATCTACCCAGCTCTGATTAGGTGGCTGAGGTTCAGAGTTCGTTGTCGAGCGCAACCACTG
GATAAAATGCACGACCCCAA

Product: glycosyl transferase, group 1 family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 401; Mature: 400

Protein sequence:

>401_residues
MTTVAYLINQYPKVSHSFIRREILALEAQGLNIRRYSIRPCPDQLIDPDDVAELDKTQVLLSVGVLGLVLALIWVMFSRP
LAFVKALGLTLKIGWHSDRGLWRHFIYLAEASVFLFWLGVAGVDHVHAHFGTNPAAVAMLCHALGGPSYSFTVHGPDEFD
KPQILHLATKVARADFVVAVSSFGQSQLYRWSNPQHWSKIHIVRCGLDQIFLHQPPTALPETPHIVCIGRLCPAKGQLLL
VEAVSQLIQEGVEIKLTLVGDGESRAAIESAIAAVHLEEVVTITGWVSSIHIRNYLLGAKVKVLPSFAEGLPVVLMEALA
LRRPVISTYIAGIPELVEPSHGWLIPAGSVTALKDAIQVSLATSTVQLQEMGQVGSERVLADHNIETEVEKLSQLFAANR
L

Sequences:

>Translated_401_residues
MTTVAYLINQYPKVSHSFIRREILALEAQGLNIRRYSIRPCPDQLIDPDDVAELDKTQVLLSVGVLGLVLALIWVMFSRP
LAFVKALGLTLKIGWHSDRGLWRHFIYLAEASVFLFWLGVAGVDHVHAHFGTNPAAVAMLCHALGGPSYSFTVHGPDEFD
KPQILHLATKVARADFVVAVSSFGQSQLYRWSNPQHWSKIHIVRCGLDQIFLHQPPTALPETPHIVCIGRLCPAKGQLLL
VEAVSQLIQEGVEIKLTLVGDGESRAAIESAIAAVHLEEVVTITGWVSSIHIRNYLLGAKVKVLPSFAEGLPVVLMEALA
LRRPVISTYIAGIPELVEPSHGWLIPAGSVTALKDAIQVSLATSTVQLQEMGQVGSERVLADHNIETEVEKLSQLFAANR
L
>Mature_400_residues
TTVAYLINQYPKVSHSFIRREILALEAQGLNIRRYSIRPCPDQLIDPDDVAELDKTQVLLSVGVLGLVLALIWVMFSRPL
AFVKALGLTLKIGWHSDRGLWRHFIYLAEASVFLFWLGVAGVDHVHAHFGTNPAAVAMLCHALGGPSYSFTVHGPDEFDK
PQILHLATKVARADFVVAVSSFGQSQLYRWSNPQHWSKIHIVRCGLDQIFLHQPPTALPETPHIVCIGRLCPAKGQLLLV
EAVSQLIQEGVEIKLTLVGDGESRAAIESAIAAVHLEEVVTITGWVSSIHIRNYLLGAKVKVLPSFAEGLPVVLMEALAL
RRPVISTYIAGIPELVEPSHGWLIPAGSVTALKDAIQVSLATSTVQLQEMGQVGSERVLADHNIETEVEKLSQLFAANRL

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1788357, Length=297, Percent_Identity=29.96632996633, Blast_Score=105, Evalue=5e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296 [H]

Pfam domain/function: PF00534 Glycos_transf_1 [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 43951; Mature: 43820

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTVAYLINQYPKVSHSFIRREILALEAQGLNIRRYSIRPCPDQLIDPDDVAELDKTQVL
CCHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHCCCCHHHHHHHHHHHH
LSVGVLGLVLALIWVMFSRPLAFVKALGLTLKIGWHSDRGLWRHFIYLAEASVFLFWLGV
HHHHHHHHHHHHHHHHHHCHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
AGVDHVHAHFGTNPAAVAMLCHALGGPSYSFTVHGPDEFDKPQILHLATKVARADFVVAV
CCHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHEEEH
SSFGQSQLYRWSNPQHWSKIHIVRCGLDQIFLHQPPTALPETPHIVCIGRLCPAKGQLLL
HHCCCHHCEECCCCCCCCEEEEEEECCHHHHCCCCCCCCCCCCCEEEEECCCCCCCCEEH
VEAVSQLIQEGVEIKLTLVGDGESRAAIESAIAAVHLEEVVTITGWVSSIHIRNYLLGAK
HHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
VKVLPSFAEGLPVVLMEALALRRPVISTYIAGIPELVEPSHGWLIPAGSVTALKDAIQVS
HEECCHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCEEEECCCHHHHHHHHHHH
LATSTVQLQEMGQVGSERVLADHNIETEVEKLSQLFAANRL
HHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TTVAYLINQYPKVSHSFIRREILALEAQGLNIRRYSIRPCPDQLIDPDDVAELDKTQVL
CHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHCCCCHHHHHHHHHHHH
LSVGVLGLVLALIWVMFSRPLAFVKALGLTLKIGWHSDRGLWRHFIYLAEASVFLFWLGV
HHHHHHHHHHHHHHHHHHCHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
AGVDHVHAHFGTNPAAVAMLCHALGGPSYSFTVHGPDEFDKPQILHLATKVARADFVVAV
CCHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHEEEH
SSFGQSQLYRWSNPQHWSKIHIVRCGLDQIFLHQPPTALPETPHIVCIGRLCPAKGQLLL
HHCCCHHCEECCCCCCCCEEEEEEECCHHHHCCCCCCCCCCCCCEEEEECCCCCCCCEEH
VEAVSQLIQEGVEIKLTLVGDGESRAAIESAIAAVHLEEVVTITGWVSSIHIRNYLLGAK
HHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
VKVLPSFAEGLPVVLMEALALRRPVISTYIAGIPELVEPSHGWLIPAGSVTALKDAIQVS
HEECCHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCEEEECCCHHHHHHHHHHH
LATSTVQLQEMGQVGSERVLADHNIETEVEKLSQLFAANRL
HHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]