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The map label for this gene is azr [H]

Identifier: 158333984

GI number: 158333984

Start: 775493

End: 776035

Strand: Direct

Name: azr [H]

Synonym: AM1_0798

Alternate gene names: 158333984

Gene position: 775493-776035 (Clockwise)

Preceding gene: 158333975

Following gene: 158333985

Centisome position: 11.92

GC content: 47.7

Gene sequence:

>543_bases
ATGACCAAAATTGTTGGCATTGGCGGTAGCCTCAGAACCGCTTCTTACAGCATGATGGCGCTAGAATTAGCAGCCGAGCG
AGTTCAAGCCCTGGGTGCTGATATTCAAATCTTGGATCTGAGAACCATGACCCTCCCTTTTTGTGATGGTGGCAAAGACT
ATTCAGAGTTTCCAGACGTTCATGTCCTGCAAGAGGTCGTCACTGCAGCCGACGGTTTAATCTTGGCTACCCCGGAATAT
CACGGCAGTGTCAGTGGGGTGATTAAGAATGCCCTGGACTTAATGGGGTTTACAGAGCTGGGCGGCAAAGTCACTGGATT
AATCAGTGTTTTGGGGGGACAGCCCAATAGCAATGCCCTTAACGAAATGCGACTAATTATGCGGTGGGTTCACGCATGGG
TCATTCCAGAACAAATTGCCATTGGTCAAGCTTGGCGGGCTTTTGATGAAGACGGAAAATTAATGGATGACAAACTAGCC
GAACGGTTAGATTCCTTTGCCCAAAGTTTGGTTACCAATACCCAAAAACTGAGTCAGAAATAG

Upstream 100 bases:

>100_bases
AGCGCCTCAGGAGGATCTATTTCTCCCGATCACGGCAAGAAAAACCAACCCAGTATTAGTTTCGTTAAACTCTGAGTAAT
CCTCTGTGAGTGAAATACCC

Downstream 100 bases:

>100_bases
TATCAAAACCAACCTTAGAATCTGTATCGGAGTGGAAAAGTGGGGCAAGCCAGGGCACTATTGTTAGTGACCCATCTCGA
TGATGGGAAAAACCATTTTT

Product: NADPH-dependent FMN reductase

Products: NAD(P)(+); FMNH(2). [C]

Alternate protein names: Azobenzene reductase [H]

Number of amino acids: Translated: 180; Mature: 179

Protein sequence:

>180_residues
MTKIVGIGGSLRTASYSMMALELAAERVQALGADIQILDLRTMTLPFCDGGKDYSEFPDVHVLQEVVTAADGLILATPEY
HGSVSGVIKNALDLMGFTELGGKVTGLISVLGGQPNSNALNEMRLIMRWVHAWVIPEQIAIGQAWRAFDEDGKLMDDKLA
ERLDSFAQSLVTNTQKLSQK

Sequences:

>Translated_180_residues
MTKIVGIGGSLRTASYSMMALELAAERVQALGADIQILDLRTMTLPFCDGGKDYSEFPDVHVLQEVVTAADGLILATPEY
HGSVSGVIKNALDLMGFTELGGKVTGLISVLGGQPNSNALNEMRLIMRWVHAWVIPEQIAIGQAWRAFDEDGKLMDDKLA
ERLDSFAQSLVTNTQKLSQK
>Mature_179_residues
TKIVGIGGSLRTASYSMMALELAAERVQALGADIQILDLRTMTLPFCDGGKDYSEFPDVHVLQEVVTAADGLILATPEYH
GSVSGVIKNALDLMGFTELGGKVTGLISVLGGQPNSNALNEMRLIMRWVHAWVIPEQIAIGQAWRAFDEDGKLMDDKLAE
RLDSFAQSLVTNTQKLSQK

Specific function: Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Requires NADPH, but not NADH, as an electron donor for its activity [H]

COG id: COG0431

COG function: function code R; Predicted flavoprotein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the azoreductase type 2 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005025 [H]

Pfam domain/function: PF03358 FMN_red [H]

EC number: 1.5.1.29 [C]

Molecular weight: Translated: 19537; Mature: 19406

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKIVGIGGSLRTASYSMMALELAAERVQALGADIQILDLRTMTLPFCDGGKDYSEFPDV
CCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEECCCCCCCHHHCCHH
HVLQEVVTAADGLILATPEYHGSVSGVIKNALDLMGFTELGGKVTGLISVLGGQPNSNAL
HHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHH
NEMRLIMRWVHAWVIPEQIAIGQAWRAFDEDGKLMDDKLAERLDSFAQSLVTNTQKLSQK
HHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TKIVGIGGSLRTASYSMMALELAAERVQALGADIQILDLRTMTLPFCDGGKDYSEFPDV
CEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEECCCCCCCHHHCCHH
HVLQEVVTAADGLILATPEYHGSVSGVIKNALDLMGFTELGGKVTGLISVLGGQPNSNAL
HHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHH
NEMRLIMRWVHAWVIPEQIAIGQAWRAFDEDGKLMDDKLAERLDSFAQSLVTNTQKLSQK
HHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD(P)H; FMN [C]

Specific reaction: NAD(P)H + FMN = NAD(P)(+) + FMNH(2). [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]