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The map label for this gene is pdhB [H]

Identifier: 158333884

GI number: 158333884

Start: 677247

End: 678230

Strand: Reverse

Name: pdhB [H]

Synonym: AM1_0696

Alternate gene names: 158333884

Gene position: 678230-677247 (Counterclockwise)

Preceding gene: 158333885

Following gene: 158333883

Centisome position: 10.43

GC content: 51.22

Gene sequence:

>984_bases
ATGGCTGAAGTACTGTTATTTAACGCCCTCCGTGACGCCATTGACGAAGAAATGGCTAATGACAACACCGTCATGGTGAT
GGGAGAAGACGTGGGCCACTATGGTGGCTCCTACAAAGTCACAAAAGGCTTGTATGACAAGTACGGAGAGCTGCGCGTCC
TCGACACCCCGATTGCAGAAAATAGCTTTACGGGCATGGCCGTTGGGGCTGCCATGACCGGCCTGAAGCCGATCATTGAA
GGCATGAATATGGGCTTTTTGCTCCTAGCCTTTAACCAAATTGCCAATAATGCCGGTATGTTGCGCTACACATCCGGCGG
CAACTTCAAAATCCCCATGGTGATTCGCGGACCGGGGGGTGTGGGTCGTCAATTGGGGGCAGAGCACTCCCAGCGCCTAG
AGGCCTATTTCCAAGCCGTGCCTGGCCTTAAAATTGTGGCTTGTTCTACCCCCTACAACGCCAAAGGATTGCTTAAAGCC
GCCATCCGTGACCCCAACCCAGTTCTCTTCTTTGAGCATGTCCTGCTCTATAACCTGAAGGAAGAATTGCCCGACCAAGA
ATACGTCCTGCCTTTAGACAAGGCCGAGGTGGTTCGCTCAGGTAAAGATGTCACCATCCTCACCTATTCTCGGATGCGTC
ACCATGTCGTGCAGGCCGCTAAAACCCTAACGGAGCAAGGTTATGATCCTGAGATTATTGATTTAATCTCCCTCAAGCCC
CTCGATTTCGATACGATTGGAGCCTCCATTCGCAAAACCCATCGCGTCATTGTCGTAGAAGAATGTATGCGGACTGGGGG
TGTGGGTGCTGAAATTATCGCCTCTATTAATGATCGATTTTTTGATGAGCTGGATGCCCCAGTGGTGCGGCTCTCTTCTC
AAGATATTCCGACGCCGTATAACGGCATGTTAGAGAGTCTAACGATTGTGCAGCCGCCTCAAATTGTGGAAGCGGTTCAG
CAGATTACGGCTCTTCAGGTCTAA

Upstream 100 bases:

>100_bases
AAGTTCCTAAGAAGCGGCTTAAAATCAGGACTCTCGATTGACACTAGGGTTGGAGGACCGTCTTCTGCTAACGTATCCAA
TAGTGTATCGCTAACGCATT

Downstream 100 bases:

>100_bases
ATTCCATTTTTACTTTTTCCTTTGGAGCAGTGTGGTGCTTAAACAACAACGCTTTTTACTAGCTCTCATTGCCCTATTAG
TGGTGGGGGCATTAATCGTC

Product: pyruvate dehydrogenase E1 component beta subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MAEVLLFNALRDAIDEEMANDNTVMVMGEDVGHYGGSYKVTKGLYDKYGELRVLDTPIAENSFTGMAVGAAMTGLKPIIE
GMNMGFLLLAFNQIANNAGMLRYTSGGNFKIPMVIRGPGGVGRQLGAEHSQRLEAYFQAVPGLKIVACSTPYNAKGLLKA
AIRDPNPVLFFEHVLLYNLKEELPDQEYVLPLDKAEVVRSGKDVTILTYSRMRHHVVQAAKTLTEQGYDPEIIDLISLKP
LDFDTIGASIRKTHRVIVVEECMRTGGVGAEIIASINDRFFDELDAPVVRLSSQDIPTPYNGMLESLTIVQPPQIVEAVQ
QITALQV

Sequences:

>Translated_327_residues
MAEVLLFNALRDAIDEEMANDNTVMVMGEDVGHYGGSYKVTKGLYDKYGELRVLDTPIAENSFTGMAVGAAMTGLKPIIE
GMNMGFLLLAFNQIANNAGMLRYTSGGNFKIPMVIRGPGGVGRQLGAEHSQRLEAYFQAVPGLKIVACSTPYNAKGLLKA
AIRDPNPVLFFEHVLLYNLKEELPDQEYVLPLDKAEVVRSGKDVTILTYSRMRHHVVQAAKTLTEQGYDPEIIDLISLKP
LDFDTIGASIRKTHRVIVVEECMRTGGVGAEIIASINDRFFDELDAPVVRLSSQDIPTPYNGMLESLTIVQPPQIVEAVQ
QITALQV
>Mature_326_residues
AEVLLFNALRDAIDEEMANDNTVMVMGEDVGHYGGSYKVTKGLYDKYGELRVLDTPIAENSFTGMAVGAAMTGLKPIIEG
MNMGFLLLAFNQIANNAGMLRYTSGGNFKIPMVIRGPGGVGRQLGAEHSQRLEAYFQAVPGLKIVACSTPYNAKGLLKAA
IRDPNPVLFFEHVLLYNLKEELPDQEYVLPLDKAEVVRSGKDVTILTYSRMRHHVVQAAKTLTEQGYDPEIIDLISLKPL
DFDTIGASIRKTHRVIVVEECMRTGGVGAEIIASINDRFFDELDAPVVRLSSQDIPTPYNGMLESLTIVQPPQIVEAVQQ
ITALQV

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:
GO:0003824: Pyruvate dehydrogenase E1 component beta subunit
GO:0008152: Pyruvate dehydrogenase E1 component beta subunit

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156564403, Length=324, Percent_Identity=40.7407407407407, Blast_Score=268, Evalue=4e-72,
Organism=Homo sapiens, GI291084858, Length=324, Percent_Identity=38.5802469135802, Blast_Score=245, Evalue=4e-65,
Organism=Homo sapiens, GI4557353, Length=320, Percent_Identity=37.1875, Blast_Score=219, Evalue=4e-57,
Organism=Homo sapiens, GI34101272, Length=320, Percent_Identity=37.1875, Blast_Score=219, Evalue=4e-57,
Organism=Caenorhabditis elegans, GI17538422, Length=319, Percent_Identity=43.2601880877743, Blast_Score=279, Evalue=1e-75,
Organism=Caenorhabditis elegans, GI17506935, Length=298, Percent_Identity=36.9127516778523, Blast_Score=187, Evalue=7e-48,
Organism=Saccharomyces cerevisiae, GI6319698, Length=324, Percent_Identity=44.1358024691358, Blast_Score=282, Evalue=4e-77,
Organism=Drosophila melanogaster, GI21358145, Length=320, Percent_Identity=43.125, Blast_Score=282, Evalue=2e-76,
Organism=Drosophila melanogaster, GI24650940, Length=320, Percent_Identity=43.125, Blast_Score=282, Evalue=2e-76,
Organism=Drosophila melanogaster, GI160714832, Length=320, Percent_Identity=37.5, Blast_Score=212, Evalue=2e-55,
Organism=Drosophila melanogaster, GI160714828, Length=320, Percent_Identity=37.5, Blast_Score=212, Evalue=3e-55,
Organism=Drosophila melanogaster, GI24650943, Length=86, Percent_Identity=50, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24650945, Length=86, Percent_Identity=50, Blast_Score=103, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR000089
- InterPro:   IPR011053
- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: =1.2.4.1 [H]

Molecular weight: Translated: 35873; Mature: 35742

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEVLLFNALRDAIDEEMANDNTVMVMGEDVGHYGGSYKVTKGLYDKYGELRVLDTPIAE
CCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEHHHHHHCCCCEEEEECCCCC
NSFTGMAVGAAMTGLKPIIEGMNMGFLLLAFNQIANNAGMLRYTSGGNFKIPMVIRGPGG
CCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEECCCCEEEEEEEECCCC
VGRQLGAEHSQRLEAYFQAVPGLKIVACSTPYNAKGLLKAAIRDPNPVLFFEHVLLYNLK
CCHHHCHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHH
EELPDQEYVLPLDKAEVVRSGKDVTILTYSRMRHHVVQAAKTLTEQGYDPEIIDLISLKP
HHCCCCCEEECCCHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCHHHEEECCC
LDFDTIGASIRKTHRVIVVEECMRTGGVGAEIIASINDRFFDELDAPVVRLSSQDIPTPY
CCHHHHHHHHHHHHEEEEHHHHHHCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCH
NGMLESLTIVQPPQIVEAVQQITALQV
HHHHHHCEECCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AEVLLFNALRDAIDEEMANDNTVMVMGEDVGHYGGSYKVTKGLYDKYGELRVLDTPIAE
CHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEHHHHHHCCCCEEEEECCCCC
NSFTGMAVGAAMTGLKPIIEGMNMGFLLLAFNQIANNAGMLRYTSGGNFKIPMVIRGPGG
CCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEECCCCEEEEEEEECCCC
VGRQLGAEHSQRLEAYFQAVPGLKIVACSTPYNAKGLLKAAIRDPNPVLFFEHVLLYNLK
CCHHHCHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHH
EELPDQEYVLPLDKAEVVRSGKDVTILTYSRMRHHVVQAAKTLTEQGYDPEIIDLISLKP
HHCCCCCEEECCCHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCHHHEEECCC
LDFDTIGASIRKTHRVIVVEECMRTGGVGAEIIASINDRFFDELDAPVVRLSSQDIPTPY
CCHHHHHHHHHHHHEEEEHHHHHHCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCH
NGMLESLTIVQPPQIVEAVQQITALQV
HHHHHHCEECCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9515924 [H]