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The map label for this gene is hsdS [C]

Identifier: 158333868

GI number: 158333868

Start: 664286

End: 665434

Strand: Reverse

Name: hsdS [C]

Synonym: AM1_0678

Alternate gene names: 158333868

Gene position: 665434-664286 (Counterclockwise)

Preceding gene: 158333869

Following gene: 158333867

Centisome position: 10.23

GC content: 43.26

Gene sequence:

>1149_bases
ATGAAGCTTAAGGAGGTTTGTAGGTTTTTAAATGGTGGAACACCAAGTAAGAAGAAGCCGGAATACTTTGAAGGGGAAAT
TCCTTGGATCACAGGTGCAGACATCAATGGGCCTATCGTTAACTCAGCTAGAAGCTATATCACTGAAGAAGCAATTCTAA
ACTCAGCAACGAAGCGTGTTCCACCAAATACAGTACTCTTAGTCACTCGAACTAGCGTAGGAAAAGTTGCTGTTTCAGGG
ATGGAGCTTTGTTATAGCCAAGACATCACATCACTGTGGCCAGATTTAGAAAAGTTAGATATTTACTATCTGACTCATTT
TCTTCGATCAAGAGAAACTTATTTAAAAGGTCAATCTCGAGGCGCGACAATCAAAGGCGTAACGAAGGGAGTCTTGGAGA
ACCTTTCTCTTCATCTTCCACCGATCGCAGAACAAAAACGGATTGCGGGGATTCTGGATGCGGCGGATGCTTTGCGGGTT
AAGAGACGAGATGCGATCTCAACTCTCGACGCCCTCCTCCAATCCACCTTCCTCACCCTCTTCGGCGACCCCATCACCAA
TCCAATGGGCTGGGATGCCAGCGACTTAGAGGCAGTTTCCGAGAAAATTACAGATGGCACACATAAAACGCCTAAATATA
CTGAATCAGGTATCGAATTTCTCTCAGCCAAAGATATTAAAAATGGTTCAATTAAATGGAATACAGGAAAATTCATTAGT
GAGGATGAGCATAAAAGCTTAATCACTAGATGTCATCCCGAGATAGGCGATGTTTTGCTAGCAAAGAGTGGTTCTCTAGG
ATCTGTGGCAATTATTGACCGAGACCATGAGTTTAGTTTGTTCGAAAGCTTATGTCTTATCAAGCATAATCGTCAGAAGA
TCGAGGCACAATTTCTTACAGCTATGCTTGAGAGTCCTAGAATGCAAATGCATTTATTGAGCAGGAACAAAGGTATTTCT
ATTAAGCATCTTCACCTGACTGATATACGAAAGCTCAAGATATTGCTTCCTCCTCTCGATAAGCAGCGTAAGTTTGCCAC
TATCGTTGCATCAATCGAAAAGCAAAAAGCGCAACAATGTGCCCACCTCGCCGAACTTGACACCCTCTTTGCCTCTCTCC
AATCCCGCGCCTTCAACGGAGAACTATAA

Upstream 100 bases:

>100_bases
AGGCGGTGGAGTATGACCCGCCAGGGGTGATTTTGAAGCGATTGGCGAAGTTGGAGAAGGAGATTGCTGAGGGGCAGCAG
GCATTGGAGAAGTTGTTGGG

Downstream 100 bases:

>100_bases
ATCCCTCATTGAGCAGAAGCTATAGTCATATACGAAGGCTTAGCAAAGAAATGACAGATGTACTCAATCAACCTGCAAAA
AGCCGAACATCAACTAGCCA

Product: type I restriction-modification enzyme S subunit

Products: NA

Alternate protein names: S.MjaXIP; Type I restriction enzyme MjaXIP specificity protein; S protein [H]

Number of amino acids: Translated: 382; Mature: 382

Protein sequence:

>382_residues
MKLKEVCRFLNGGTPSKKKPEYFEGEIPWITGADINGPIVNSARSYITEEAILNSATKRVPPNTVLLVTRTSVGKVAVSG
MELCYSQDITSLWPDLEKLDIYYLTHFLRSRETYLKGQSRGATIKGVTKGVLENLSLHLPPIAEQKRIAGILDAADALRV
KRRDAISTLDALLQSTFLTLFGDPITNPMGWDASDLEAVSEKITDGTHKTPKYTESGIEFLSAKDIKNGSIKWNTGKFIS
EDEHKSLITRCHPEIGDVLLAKSGSLGSVAIIDRDHEFSLFESLCLIKHNRQKIEAQFLTAMLESPRMQMHLLSRNKGIS
IKHLHLTDIRKLKILLPPLDKQRKFATIVASIEKQKAQQCAHLAELDTLFASLQSRAFNGEL

Sequences:

>Translated_382_residues
MKLKEVCRFLNGGTPSKKKPEYFEGEIPWITGADINGPIVNSARSYITEEAILNSATKRVPPNTVLLVTRTSVGKVAVSG
MELCYSQDITSLWPDLEKLDIYYLTHFLRSRETYLKGQSRGATIKGVTKGVLENLSLHLPPIAEQKRIAGILDAADALRV
KRRDAISTLDALLQSTFLTLFGDPITNPMGWDASDLEAVSEKITDGTHKTPKYTESGIEFLSAKDIKNGSIKWNTGKFIS
EDEHKSLITRCHPEIGDVLLAKSGSLGSVAIIDRDHEFSLFESLCLIKHNRQKIEAQFLTAMLESPRMQMHLLSRNKGIS
IKHLHLTDIRKLKILLPPLDKQRKFATIVASIEKQKAQQCAHLAELDTLFASLQSRAFNGEL
>Mature_382_residues
MKLKEVCRFLNGGTPSKKKPEYFEGEIPWITGADINGPIVNSARSYITEEAILNSATKRVPPNTVLLVTRTSVGKVAVSG
MELCYSQDITSLWPDLEKLDIYYLTHFLRSRETYLKGQSRGATIKGVTKGVLENLSLHLPPIAEQKRIAGILDAADALRV
KRRDAISTLDALLQSTFLTLFGDPITNPMGWDASDLEAVSEKITDGTHKTPKYTESGIEFLSAKDIKNGSIKWNTGKFIS
EDEHKSLITRCHPEIGDVLLAKSGSLGSVAIIDRDHEFSLFESLCLIKHNRQKIEAQFLTAMLESPRMQMHLLSRNKGIS
IKHLHLTDIRKLKILLPPLDKQRKFATIVASIEKQKAQQCAHLAELDTLFASLQSRAFNGEL

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0732

COG function: function code V; Restriction endonuclease S subunits

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type-I restriction system S methylase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000055 [H]

Pfam domain/function: PF01420 Methylase_S [H]

EC number: NA

Molecular weight: Translated: 42563; Mature: 42563

Theoretical pI: Translated: 9.10; Mature: 9.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKEVCRFLNGGTPSKKKPEYFEGEIPWITGADINGPIVNSARSYITEEAILNSATKRV
CCHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCC
PPNTVLLVTRTSVGKVAVSGMELCYSQDITSLWPDLEKLDIYYLTHFLRSRETYLKGQSR
CCCEEEEEEECCCCHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCC
GATIKGVTKGVLENLSLHLPPIAEQKRIAGILDAADALRVKRRDAISTLDALLQSTFLTL
CCEEHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FGDPITNPMGWDASDLEAVSEKITDGTHKTPKYTESGIEFLSAKDIKNGSIKWNTGKFIS
HCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCEEECCCCCCC
EDEHKSLITRCHPEIGDVLLAKSGSLGSVAIIDRDHEFSLFESLCLIKHNRQKIEAQFLT
CHHHHHHHHHHCCCHHCEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
AMLESPRMQMHLLSRNKGISIKHLHLTDIRKLKILLPPLDKQRKFATIVASIEKQKAQQC
HHHHCCCCEEHHHHCCCCCEEEEEEHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHH
AHLAELDTLFASLQSRAFNGEL
HHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKLKEVCRFLNGGTPSKKKPEYFEGEIPWITGADINGPIVNSARSYITEEAILNSATKRV
CCHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCC
PPNTVLLVTRTSVGKVAVSGMELCYSQDITSLWPDLEKLDIYYLTHFLRSRETYLKGQSR
CCCEEEEEEECCCCHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCC
GATIKGVTKGVLENLSLHLPPIAEQKRIAGILDAADALRVKRRDAISTLDALLQSTFLTL
CCEEHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FGDPITNPMGWDASDLEAVSEKITDGTHKTPKYTESGIEFLSAKDIKNGSIKWNTGKFIS
HCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCEEECCCCCCC
EDEHKSLITRCHPEIGDVLLAKSGSLGSVAIIDRDHEFSLFESLCLIKHNRQKIEAQFLT
CHHHHHHHHHHCCCHHCEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
AMLESPRMQMHLLSRNKGISIKHLHLTDIRKLKILLPPLDKQRKFATIVASIEKQKAQQC
HHHHCCCCEEHHHHCCCCCEEEEEEHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHH
AHLAELDTLFASLQSRAFNGEL
HHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]