Click here to switch to the map view.

The map label for this gene is hsdM [H]

Identifier: 158333869

GI number: 158333869

Start: 665431

End: 666576

Strand: Reverse

Name: hsdM [H]

Synonym: AM1_0679

Alternate gene names: 158333869

Gene position: 666576-665431 (Counterclockwise)

Preceding gene: 158333871

Following gene: 158333868

Centisome position: 10.25

GC content: 52.62

Gene sequence:

>1146_bases
ATGAAGGATGCGCTGTTTATGATGCCGACGGCGCGGGTGTTGGCGAATGTGGTGGATCAGCTGGACGCTATTGAAATGGC
GGATCGCGATACGAAGGGCGATCTATATGAGTATATGCTGGGCAAGATTGCTAGTGCGGGGCAGAATGGGCAGTTTCGGA
CACCGCGCCACATTATTAAGCTAATGGTGGAGTTGACGGCTCCAACGCCGAAGGATGTGATTTGTGATCCGGCCTGTGGG
ACGGCGGGCTTTTTGATTGCGGCATCGGAATATTTGATGGATCACCACAGTGATGTGATTTATAAGGATGCGGAGTCTCG
GCGACGGTTTAATGAGGAGACGTTTTCCGGGTACGACTTTGATTCGACGATGCTGCGGATTGGCAGTATGAATATGCTGC
TGCACGGGGTAGAGAAGCCAGATATTCGCTATAAGGATTCGTTGGCTGAGGCGGATGCAGCGGCGGGTGATGATGAGGAA
GCCTATTCGTTGATTCTGGCGAATCCTCCGTTTGCGGGGAGTTTGGACTATGAGACGACGGCGAAGGATCTGTTGAAGGT
GGTGAAGACCAAGAAAACAGAGCTGTTGTTTTTGACGTTGTTTTTGCGGGTGTTGCAGACGGGGGGACGAGCGGCGGTGA
TTGTGCCGGATGGGGTGTTGTTTGGTTCGTCAAAAGCCCACAAAGCGCTGCGGAAGATGTTGGTGGAAGATCAGAAGCTG
GATGGGATTATTTCGATGCCGTCGGGGGTGTTTAAGCCCTATGCGGGGGTGTCGACGGCGATTGTGTTGTTTACGAAGAC
CAATTCTGGTGGGACGGATCAGGTGTGGTTTTATGACATGCAGACAGATGGGTTTAGCTTGGATGATAAGCGGACGGAGC
AGCCGGATAAGAGTGATTTGCCGGATATTTTGACGCGGTGGCAGGCTCGGGAGGCGGAGGCTGAGCGGAAGCGGACGGAG
CAGAGCTTTTTGGTGCCGAGGGAAGAGATTGTGGAGAATGATTATGATTTGTCGATTAATCGGTATAAGGAGGTGGTTTA
TGAGGCGGTGGAGTATGACCCGCCAGGGGTGATTTTGAAGCGATTGGCGAAGTTGGAGAAGGAGATTGCTGAGGGGCAGC
AGGCATTGGAGAAGTTGTTGGGATGA

Upstream 100 bases:

>100_bases
ATGGTTGGTTCTGGACTTCTTGAAGCTGACATCACTCATTAGAAAAATAGAAACTGAGAATAGTCTATTTTTTATCTGTC
TAAAGTGATAAAAGAGGGAT

Downstream 100 bases:

>100_bases
AGCTTAAGGAGGTTTGTAGGTTTTTAAATGGTGGAACACCAAGTAAGAAGAAGCCGGAATACTTTGAAGGGGAAATTCCT
TGGATCACAGGTGCAGACAT

Product: type I restriction modification system M subunit

Products: NA

Alternate protein names: M.EcoEI [H]

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
MKDALFMMPTARVLANVVDQLDAIEMADRDTKGDLYEYMLGKIASAGQNGQFRTPRHIIKLMVELTAPTPKDVICDPACG
TAGFLIAASEYLMDHHSDVIYKDAESRRRFNEETFSGYDFDSTMLRIGSMNMLLHGVEKPDIRYKDSLAEADAAAGDDEE
AYSLILANPPFAGSLDYETTAKDLLKVVKTKKTELLFLTLFLRVLQTGGRAAVIVPDGVLFGSSKAHKALRKMLVEDQKL
DGIISMPSGVFKPYAGVSTAIVLFTKTNSGGTDQVWFYDMQTDGFSLDDKRTEQPDKSDLPDILTRWQAREAEAERKRTE
QSFLVPREEIVENDYDLSINRYKEVVYEAVEYDPPGVILKRLAKLEKEIAEGQQALEKLLG

Sequences:

>Translated_381_residues
MKDALFMMPTARVLANVVDQLDAIEMADRDTKGDLYEYMLGKIASAGQNGQFRTPRHIIKLMVELTAPTPKDVICDPACG
TAGFLIAASEYLMDHHSDVIYKDAESRRRFNEETFSGYDFDSTMLRIGSMNMLLHGVEKPDIRYKDSLAEADAAAGDDEE
AYSLILANPPFAGSLDYETTAKDLLKVVKTKKTELLFLTLFLRVLQTGGRAAVIVPDGVLFGSSKAHKALRKMLVEDQKL
DGIISMPSGVFKPYAGVSTAIVLFTKTNSGGTDQVWFYDMQTDGFSLDDKRTEQPDKSDLPDILTRWQAREAEAERKRTE
QSFLVPREEIVENDYDLSINRYKEVVYEAVEYDPPGVILKRLAKLEKEIAEGQQALEKLLG
>Mature_381_residues
MKDALFMMPTARVLANVVDQLDAIEMADRDTKGDLYEYMLGKIASAGQNGQFRTPRHIIKLMVELTAPTPKDVICDPACG
TAGFLIAASEYLMDHHSDVIYKDAESRRRFNEETFSGYDFDSTMLRIGSMNMLLHGVEKPDIRYKDSLAEADAAAGDDEE
AYSLILANPPFAGSLDYETTAKDLLKVVKTKKTELLFLTLFLRVLQTGGRAAVIVPDGVLFGSSKAHKALRKMLVEDQKL
DGIISMPSGVFKPYAGVSTAIVLFTKTNSGGTDQVWFYDMQTDGFSLDDKRTEQPDKSDLPDILTRWQAREAEAERKRTE
QSFLVPREEIVENDYDLSINRYKEVVYEAVEYDPPGVILKRLAKLEKEIAEGQQALEKLLG

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0286

COG function: function code V; Type I restriction-modification system methyltransferase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1790808, Length=362, Percent_Identity=30.939226519337, Blast_Score=142, Evalue=4e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022749
- InterPro:   IPR003356
- InterPro:   IPR002052
- InterPro:   IPR002296 [H]

Pfam domain/function: PF12161 HsdM_N; PF02384 N6_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 42605; Mature: 42605

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDALFMMPTARVLANVVDQLDAIEMADRDTKGDLYEYMLGKIASAGQNGQFRTPRHIIK
CCCCEEECCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHH
LMVELTAPTPKDVICDPACGTAGFLIAASEYLMDHHSDVIYKDAESRRRFNEETFSGYDF
HHHHHCCCCCCCCEECCCCCCHHHHHHHHHHHHHCCCCCEECCHHHHHHCCHHHCCCCCC
DSTMLRIGSMNMLLHGVEKPDIRYKDSLAEADAAAGDDEEAYSLILANPPFAGSLDYETT
CHHHHHHCCHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCHHEEEEEECCCCCCCCCCHHH
AKDLLKVVKTKKTELLFLTLFLRVLQTGGRAAVIVPDGVLFGSSKAHKALRKMLVEDQKL
HHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEECCCEEECCHHHHHHHHHHHHHHHHC
DGIISMPSGVFKPYAGVSTAIVLFTKTNSGGTDQVWFYDMQTDGFSLDDKRTEQPDKSDL
CCEEECCCCCCCCCCCCCEEEEEEEECCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCC
PDILTRWQAREAEAERKRTEQSFLVPREEIVENDYDLSINRYKEVVYEAVEYDPPGVILK
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHH
RLAKLEKEIAEGQQALEKLLG
HHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKDALFMMPTARVLANVVDQLDAIEMADRDTKGDLYEYMLGKIASAGQNGQFRTPRHIIK
CCCCEEECCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHH
LMVELTAPTPKDVICDPACGTAGFLIAASEYLMDHHSDVIYKDAESRRRFNEETFSGYDF
HHHHHCCCCCCCCEECCCCCCHHHHHHHHHHHHHCCCCCEECCHHHHHHCCHHHCCCCCC
DSTMLRIGSMNMLLHGVEKPDIRYKDSLAEADAAAGDDEEAYSLILANPPFAGSLDYETT
CHHHHHHCCHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCHHEEEEEECCCCCCCCCCHHH
AKDLLKVVKTKKTELLFLTLFLRVLQTGGRAAVIVPDGVLFGSSKAHKALRKMLVEDQKL
HHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEECCCEEECCHHHHHHHHHHHHHHHHC
DGIISMPSGVFKPYAGVSTAIVLFTKTNSGGTDQVWFYDMQTDGFSLDDKRTEQPDKSDL
CCEEECCCCCCCCCCCCCEEEEEEEECCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCC
PDILTRWQAREAEAERKRTEQSFLVPREEIVENDYDLSINRYKEVVYEAVEYDPPGVILK
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHH
RLAKLEKEIAEGQQALEKLLG
HHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8412658 [H]