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The map label for this gene is glyS

Identifier: 158333790

GI number: 158333790

Start: 584794

End: 586932

Strand: Direct

Name: glyS

Synonym: AM1_0598

Alternate gene names: 158333790

Gene position: 584794-586932 (Clockwise)

Preceding gene: 158333784

Following gene: 158333791

Centisome position: 8.99

GC content: 51.19

Gene sequence:

>2139_bases
ATGGCGACGTTTTTATTAGAAGTAGGGACAGAAGAGTTGCCCGCAAGTTTTGTGGAGTCGGCGATTCAGCAATGGCGATC
GCACATCCCAAACAGCCTAGAGGAACACCTGCTCACCCCCCAATCTCTGAAGGTTTACGGCACCCCTCGACGACTGGCTA
TTTTGGCAGAAGGCCTGCCAGACCAGCAGCCGGACCAAACGGTCGAAGTCAAAGGTCCCCCAGCAAAAGCTGCGTTTAAG
GATGGCAAACCCACCAAAGCGGCTGAAGGATTTGCCCGCAAACAGGGGGTTGAGATTAAAGATTTTGAGCTGCGCGATAC
GGAAAAAGGCGAATTTATTTTTGTTCAGAAAACGACTCTGGGTAAACCGGCGGTTGATGTTTTAGCCGACCTAATCCCCA
CCTGGATTTTTAGCCTAGAAGGCAAGCGGTTTATGCGTTGGAGCGATGGCGAGGTAAAGTTCTCCCGCCCCATTCGCTGG
TTGGTCTCGTTGCTGGACGACCATCTTATACCTATTAAATTGGAGAATTCAACAGGTACGATATCCAGCGATCGCACTTC
TTCAGGCCATCGAGTTCTGCATCCAGACCCAGTAACGATTAAGCATCCCCAAGACTATGTGGCGACCTTAGAAAAGGCTT
CCGTTCAGGTGGATCCAGAGCAGCGTCAAACCCTGATCAAAGAACAGGTTCACGCCTGTGCTAAGGCCGTTAAAGGTAAG
GCCGTTATTTCTGAAGATCTGCTGGCGGAAGTCACCTATTTAGTGGAATGGCCGACGGCCGTGGTGGGAGGTTTTGAGTC
AGAATTTTTGGAACTGCCTGCTGAGGTGATTACAACGGAGATGGTCAGCCATCAGCGCTATTTCCCGGTGGAGGCCCCCA
AGGGCGCGAAGCTCTTGCCCCACTTCATTACTATTTCCAATGGTGATCCAGCCAAATCAGACATTATTTCATCGGGCAAT
GAACGGGTGATTCGGGCTCGATTAGCCGATGGTCAGTTCTTCTATAAGGCTGACCAGGCCATGCCCTTAGCAGAGTATTT
GCCCAAGCTAGAGACGGTTACCTTCCAAGAAGATTTGGGGTCTGTTCGGGCCAAGGTGGATCGCATCCAGGCCAATGCCC
TGTGGGTTGCAAAACAACTCCAGCTCGATCAGAAGGTGACTCAGCAAGTCGAGCGGGCAGCCCAGCTTTGTAAGGCGGAT
CTCGTAACCCAAATGGTGGGCGAGTTTCCAGAACTGCAGGGGGTGATGGGGGAGAAATATGCGATCGCAAGTGGTGAACC
CACCGAGATTGCCACTGCCATCGTCGAGCATTACCTGCCCAAAGGGGCCAATGACCAGCTTCCACAGACCCTGGTGGGGC
AAATTGTTGGTATTGCCGATCGTCTGGATACCCTGGTCAGTATTTTTGGTCTGGGGATGATACCCACAGGGTCTTCTGAC
CCCTTTGCCCTGCGCCGAGCAGCCAACGCCATTCTCAATATTGTCTGGGCTGCCAATCTAGATCTCAATCTAGACCAACT
CCTGACCGAAACGACTGCTGCCTTTGCTCAGACCTTTGCTAAATTTCAGCCTGAGGTCCAACAGCTTCGAGACTTCTTTG
GTCAGCGTCTCCGTAGCCTCCTGCAAGATGAGCAGACTATTGACTATGACTTGGTCAATGCCGTTTTAGGAGAAAAGGGC
GAGCATACTCAGCGAGTCCTAGTGGATGTGTTAGATGGACGCGATCGCGCCCAGTATCTGCAGACGATTCGCACCAACGG
CACCTTGGATACGATCTATGAAACCGTCAATCGAGCTGCTCGCCTAGCTACCAAAGGAGATCTAGCCTCAGATGTACTGG
ATCCGAAGGGAGTAGTTCAGCCAAAGCTGTTTGAACAGTCTTCTGAGCAGGTCTTTTATGATGGCTTACTCACCCTTTTA
CCTCAAACACAAGCGGCCCAGTCTTCGCGAGACTATCAAAAGCTGGTTCAAGGCTTAACCAAAATTGCTCCATCCGTTAG
CCAATTTTTTGATGGCGACGATAGTGTCTTAGTAATAGCGGAAGATGCCGATTTACGAGCGAATCGCTTAAATTTACTAG
GTCTATTACGAAATCACGCCCTCGTGCTCGGGGACTTCGGTGCGATTGTCAAAAGCTAG

Upstream 100 bases:

>100_bases
CTTGCCTAAACCTCTTCCCATTTTGCTGTAGTCCCACGATAGACTGTGTAAGCAAACCTTTGGTCTGCATATATTCGGCA
ATATTCCAGCGGGGTCGGTG

Downstream 100 bases:

>100_bases
AATATAGAAGAACGCACAGTGCCTTTGCCTCCATGGCAACTCCCCCATTACTGCATCCCTCCTGCCACCGTACCCCTGAA
GGGTTAACGGTTATTGCCGA

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS

Number of amino acids: Translated: 712; Mature: 711

Protein sequence:

>712_residues
MATFLLEVGTEELPASFVESAIQQWRSHIPNSLEEHLLTPQSLKVYGTPRRLAILAEGLPDQQPDQTVEVKGPPAKAAFK
DGKPTKAAEGFARKQGVEIKDFELRDTEKGEFIFVQKTTLGKPAVDVLADLIPTWIFSLEGKRFMRWSDGEVKFSRPIRW
LVSLLDDHLIPIKLENSTGTISSDRTSSGHRVLHPDPVTIKHPQDYVATLEKASVQVDPEQRQTLIKEQVHACAKAVKGK
AVISEDLLAEVTYLVEWPTAVVGGFESEFLELPAEVITTEMVSHQRYFPVEAPKGAKLLPHFITISNGDPAKSDIISSGN
ERVIRARLADGQFFYKADQAMPLAEYLPKLETVTFQEDLGSVRAKVDRIQANALWVAKQLQLDQKVTQQVERAAQLCKAD
LVTQMVGEFPELQGVMGEKYAIASGEPTEIATAIVEHYLPKGANDQLPQTLVGQIVGIADRLDTLVSIFGLGMIPTGSSD
PFALRRAANAILNIVWAANLDLNLDQLLTETTAAFAQTFAKFQPEVQQLRDFFGQRLRSLLQDEQTIDYDLVNAVLGEKG
EHTQRVLVDVLDGRDRAQYLQTIRTNGTLDTIYETVNRAARLATKGDLASDVLDPKGVVQPKLFEQSSEQVFYDGLLTLL
PQTQAAQSSRDYQKLVQGLTKIAPSVSQFFDGDDSVLVIAEDADLRANRLNLLGLLRNHALVLGDFGAIVKS

Sequences:

>Translated_712_residues
MATFLLEVGTEELPASFVESAIQQWRSHIPNSLEEHLLTPQSLKVYGTPRRLAILAEGLPDQQPDQTVEVKGPPAKAAFK
DGKPTKAAEGFARKQGVEIKDFELRDTEKGEFIFVQKTTLGKPAVDVLADLIPTWIFSLEGKRFMRWSDGEVKFSRPIRW
LVSLLDDHLIPIKLENSTGTISSDRTSSGHRVLHPDPVTIKHPQDYVATLEKASVQVDPEQRQTLIKEQVHACAKAVKGK
AVISEDLLAEVTYLVEWPTAVVGGFESEFLELPAEVITTEMVSHQRYFPVEAPKGAKLLPHFITISNGDPAKSDIISSGN
ERVIRARLADGQFFYKADQAMPLAEYLPKLETVTFQEDLGSVRAKVDRIQANALWVAKQLQLDQKVTQQVERAAQLCKAD
LVTQMVGEFPELQGVMGEKYAIASGEPTEIATAIVEHYLPKGANDQLPQTLVGQIVGIADRLDTLVSIFGLGMIPTGSSD
PFALRRAANAILNIVWAANLDLNLDQLLTETTAAFAQTFAKFQPEVQQLRDFFGQRLRSLLQDEQTIDYDLVNAVLGEKG
EHTQRVLVDVLDGRDRAQYLQTIRTNGTLDTIYETVNRAARLATKGDLASDVLDPKGVVQPKLFEQSSEQVFYDGLLTLL
PQTQAAQSSRDYQKLVQGLTKIAPSVSQFFDGDDSVLVIAEDADLRANRLNLLGLLRNHALVLGDFGAIVKS
>Mature_711_residues
ATFLLEVGTEELPASFVESAIQQWRSHIPNSLEEHLLTPQSLKVYGTPRRLAILAEGLPDQQPDQTVEVKGPPAKAAFKD
GKPTKAAEGFARKQGVEIKDFELRDTEKGEFIFVQKTTLGKPAVDVLADLIPTWIFSLEGKRFMRWSDGEVKFSRPIRWL
VSLLDDHLIPIKLENSTGTISSDRTSSGHRVLHPDPVTIKHPQDYVATLEKASVQVDPEQRQTLIKEQVHACAKAVKGKA
VISEDLLAEVTYLVEWPTAVVGGFESEFLELPAEVITTEMVSHQRYFPVEAPKGAKLLPHFITISNGDPAKSDIISSGNE
RVIRARLADGQFFYKADQAMPLAEYLPKLETVTFQEDLGSVRAKVDRIQANALWVAKQLQLDQKVTQQVERAAQLCKADL
VTQMVGEFPELQGVMGEKYAIASGEPTEIATAIVEHYLPKGANDQLPQTLVGQIVGIADRLDTLVSIFGLGMIPTGSSDP
FALRRAANAILNIVWAANLDLNLDQLLTETTAAFAQTFAKFQPEVQQLRDFFGQRLRSLLQDEQTIDYDLVNAVLGEKGE
HTQRVLVDVLDGRDRAQYLQTIRTNGTLDTIYETVNRAARLATKGDLASDVLDPKGVVQPKLFEQSSEQVFYDGLLTLLP
QTQAAQSSRDYQKLVQGLTKIAPSVSQFFDGDDSVLVIAEDADLRANRLNLLGLLRNHALVLGDFGAIVKS

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:
GO:0000166: Glycyl-tRNA synthetase beta subunit
GO:0004812: Glycyl-tRNA synthetase beta subunit
GO:0004820: Glycyl-tRNA synthetase beta subunit
GO:0005524: Glycyl-tRNA synthetase beta subunit
GO:0005737: Glycyl-tRNA synthetase beta subunit
GO:0006412: Glycyl-tRNA synthetase beta subunit
GO:0006426: Glycyl-tRNA synthetase beta subunit
GO:0016874: Glycyl-tRNA synthetase beta subunit

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Escherichia coli, GI1789982, Length=707, Percent_Identity=35.5021216407355, Blast_Score=393, Evalue=1e-110,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYGB_ACAM1 (B0CD57)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001514962.1
- ProteinModelPortal:   B0CD57
- GeneID:   5679426
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_0598
- HOGENOM:   HBG586599
- OMA:   SNITYGH
- ProtClustDB:   PRK01233
- BioCyc:   AMAR329726:AM1_0598-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00255
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311
- PRINTS:   PR01045
- TIGRFAMs:   TIGR00211

Pfam domain/function: PF02092 tRNA_synt_2f

EC number: =6.1.1.14

Molecular weight: Translated: 78677; Mature: 78545

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATFLLEVGTEELPASFVESAIQQWRSHIPNSLEEHLLTPQSLKVYGTPRRLAILAEGLP
CCEEEEECCCHHCCHHHHHHHHHHHHHHCCCHHHHHCCCCCCEEEECCCHHHHHEECCCC
DQQPDQTVEVKGPPAKAAFKDGKPTKAAEGFARKQGVEIKDFELRDTEKGEFIFVQKTTL
CCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHCCCCEECCCCCCCCCCCEEEEEECCC
GKPAVDVLADLIPTWIFSLEGKRFMRWSDGEVKFSRPIRWLVSLLDDHLIPIKLENSTGT
CCHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEHHHHHHHHHHHHCCEEEEEECCCCCC
ISSDRTSSGHRVLHPDPVTIKHPQDYVATLEKASVQVDPEQRQTLIKEQVHACAKAVKGK
CCCCCCCCCCEEECCCCCEEECCHHHHHHHHHCCEEECHHHHHHHHHHHHHHHHHHHCCC
AVISEDLLAEVTYLVEWPTAVVGGFESEFLELPAEVITTEMVSHQRYFPVEAPKGAKLLP
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCC
HFITISNGDPAKSDIISSGNERVIRARLADGQFFYKADQAMPLAEYLPKLETVTFQEDLG
EEEEECCCCCCHHHHHCCCCCEEEEEEECCCEEEEECCCCCCHHHHCCCHHEEEEHHHHH
SVRAKVDRIQANALWVAKQLQLDQKVTQQVERAAQLCKADLVTQMVGEFPELQGVMGEKY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCE
AIASGEPTEIATAIVEHYLPKGANDQLPQTLVGQIVGIADRLDTLVSIFGLGMIPTGSSD
EECCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
PFALRRAANAILNIVWAANLDLNLDQLLTETTAAFAQTFAKFQPEVQQLRDFFGQRLRSL
HHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
LQDEQTIDYDLVNAVLGEKGEHTQRVLVDVLDGRDRAQYLQTIRTNGTLDTIYETVNRAA
HCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHH
RLATKGDLASDVLDPKGVVQPKLFEQSSEQVFYDGLLTLLPQTQAAQSSRDYQKLVQGLT
HHHCCCCHHHHHCCCCCCCCCHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
KIAPSVSQFFDGDDSVLVIAEDADLRANRLNLLGLLRNHALVLGDFGAIVKS
HHCHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHCCEEEEECHHHHHCC
>Mature Secondary Structure 
ATFLLEVGTEELPASFVESAIQQWRSHIPNSLEEHLLTPQSLKVYGTPRRLAILAEGLP
CEEEEECCCHHCCHHHHHHHHHHHHHHCCCHHHHHCCCCCCEEEECCCHHHHHEECCCC
DQQPDQTVEVKGPPAKAAFKDGKPTKAAEGFARKQGVEIKDFELRDTEKGEFIFVQKTTL
CCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHCCCCEECCCCCCCCCCCEEEEEECCC
GKPAVDVLADLIPTWIFSLEGKRFMRWSDGEVKFSRPIRWLVSLLDDHLIPIKLENSTGT
CCHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEHHHHHHHHHHHHCCEEEEEECCCCCC
ISSDRTSSGHRVLHPDPVTIKHPQDYVATLEKASVQVDPEQRQTLIKEQVHACAKAVKGK
CCCCCCCCCCEEECCCCCEEECCHHHHHHHHHCCEEECHHHHHHHHHHHHHHHHHHHCCC
AVISEDLLAEVTYLVEWPTAVVGGFESEFLELPAEVITTEMVSHQRYFPVEAPKGAKLLP
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCC
HFITISNGDPAKSDIISSGNERVIRARLADGQFFYKADQAMPLAEYLPKLETVTFQEDLG
EEEEECCCCCCHHHHHCCCCCEEEEEEECCCEEEEECCCCCCHHHHCCCHHEEEEHHHHH
SVRAKVDRIQANALWVAKQLQLDQKVTQQVERAAQLCKADLVTQMVGEFPELQGVMGEKY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCE
AIASGEPTEIATAIVEHYLPKGANDQLPQTLVGQIVGIADRLDTLVSIFGLGMIPTGSSD
EECCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
PFALRRAANAILNIVWAANLDLNLDQLLTETTAAFAQTFAKFQPEVQQLRDFFGQRLRSL
HHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
LQDEQTIDYDLVNAVLGEKGEHTQRVLVDVLDGRDRAQYLQTIRTNGTLDTIYETVNRAA
HCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHH
RLATKGDLASDVLDPKGVVQPKLFEQSSEQVFYDGLLTLLPQTQAAQSSRDYQKLVQGLT
HHHCCCCHHHHHCCCCCCCCCHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
KIAPSVSQFFDGDDSVLVIAEDADLRANRLNLLGLLRNHALVLGDFGAIVKS
HHCHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHCCEEEEECHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA