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The map label for this gene is yfiJ [H]

Identifier: 158333710

GI number: 158333710

Start: 489385

End: 490653

Strand: Reverse

Name: yfiJ [H]

Synonym: AM1_0516

Alternate gene names: 158333710

Gene position: 490653-489385 (Counterclockwise)

Preceding gene: 158333711

Following gene: 158333709

Centisome position: 7.54

GC content: 50.04

Gene sequence:

>1269_bases
ATGTCTCGGTTGTTTCGTTCCAACCATCCATTTCACTTACTGCTGTATTTGGAATGGATTTTGTTGGGAGTTGCTCTATT
AACGTTGTTTGTACCTTTTCCTCGCCACCATTTTCTCCTGCCCCACTTTACGCCAAGGGGAGCGTTAAGGTTTCCCTTGG
GCATCTTCCTGAGTATTCTGGCATTAGCGGGTTTAGGCCTTAAACTCCCCATCCATCAATCTCAAAAGGTGAAAGTGCTG
TATACCTGTTTGGGCTTTCTGCTCTGTTGGGCAACAGTGTTTATGGGAGGGCGGTTTCGAGACTTCTTCACCCCGATGCT
CTTAATTGTGGTGATTCGAGCCTGCTTACTGTTTCCTTGTCGGCAGCGATTGATAGCAGCCTCTGTTGCCTATGGCTCTT
TTCTTGTCCGCTTAATGACGGGGGCCCAGCGGATGCGTCCATTTCCCAGGTTAGGGCCTCCACCGCCTTGGCGGAGCAGA
GGACTGCCCCAAGAATTACTGGCGAGCTTGCAGATCAGAAGTGCACTTTTATTTGGGTTAGTCTTGGTCTTTGTCTTCCT
GATGGTAGGGGCGCTTTTGACTGAGCGTCAGAGTCGGACAGAATTAGCAGCTGCGAACCAACGCCTGCGGCAATATGCCC
TAATGATCGAAAATCAAGCCACTCTACAGGAGCGGAACCGAATTGCCCGGGAAATGCATGATTCCGTTGGCCATTCCCTC
ACGGCTCAAAGTATTCAGCTGGAGAATGTGGATGTATGGATGGCCTCTGATCGGGCCAAAGCCACAGATCACCTGGCGAA
AGCGCGATCTTTGGGGAAAGAGGCTCTTCAAAATGTCCGCCATACCGTTGCCACCTTGCGGAATAACCCCCTACAAGGTC
AATCCTTGTCCCAAGCGCTATCCCACCTAACCCAAGAGTTTGAACGAACCACATCGATTCAGGTCCAATTCTCTACCCAC
CTTGAGATGCCAGTCCCTCGAGAAATGGCTGCAGCCCTCTATCGTTTAGTGCAAGAAGCCCTCACTAATGTTTCAAAACA
CAGCGAAGCGACCCAAGTTACGATTGAGTTGACGGAGCAAGAAATGGAACTGTCCCTCAACATTTCTGACAATGGCAAAG
GGTTTCTGCCAGAGAAAAATACAACAGGCTTTGGTCTCCAAGGGATGCAGGAACGGACAGTAGCCTTGGGCGGCCTATTC
GAACTCTGGAGCCAACCTCAAACAGGCTGCCATATTCAGATCAAGATTCCTCGATTAGGAGAAGAATAA

Upstream 100 bases:

>100_bases
AAGCGCTGGGAGACAAACCAACCTAGAAACCGTCGCCTATAGTGGGAGAACATCAGAGCCCTAGCGGCCTGGTAAATCCA
TTCCTGGACTGGGAGCCTAT

Downstream 100 bases:

>100_bases
TGATTCGAGTGATGTTGGTAGATGATCAAGGGATTGTGCGGGAAGGGCTCTCCAGTCTGCTCAACACCAAACCCGATTTA
GAAGTCGTGGGTGAGGCAGA

Product: two-component sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 422; Mature: 421

Protein sequence:

>422_residues
MSRLFRSNHPFHLLLYLEWILLGVALLTLFVPFPRHHFLLPHFTPRGALRFPLGIFLSILALAGLGLKLPIHQSQKVKVL
YTCLGFLLCWATVFMGGRFRDFFTPMLLIVVIRACLLFPCRQRLIAASVAYGSFLVRLMTGAQRMRPFPRLGPPPPWRSR
GLPQELLASLQIRSALLFGLVLVFVFLMVGALLTERQSRTELAAANQRLRQYALMIENQATLQERNRIAREMHDSVGHSL
TAQSIQLENVDVWMASDRAKATDHLAKARSLGKEALQNVRHTVATLRNNPLQGQSLSQALSHLTQEFERTTSIQVQFSTH
LEMPVPREMAAALYRLVQEALTNVSKHSEATQVTIELTEQEMELSLNISDNGKGFLPEKNTTGFGLQGMQERTVALGGLF
ELWSQPQTGCHIQIKIPRLGEE

Sequences:

>Translated_422_residues
MSRLFRSNHPFHLLLYLEWILLGVALLTLFVPFPRHHFLLPHFTPRGALRFPLGIFLSILALAGLGLKLPIHQSQKVKVL
YTCLGFLLCWATVFMGGRFRDFFTPMLLIVVIRACLLFPCRQRLIAASVAYGSFLVRLMTGAQRMRPFPRLGPPPPWRSR
GLPQELLASLQIRSALLFGLVLVFVFLMVGALLTERQSRTELAAANQRLRQYALMIENQATLQERNRIAREMHDSVGHSL
TAQSIQLENVDVWMASDRAKATDHLAKARSLGKEALQNVRHTVATLRNNPLQGQSLSQALSHLTQEFERTTSIQVQFSTH
LEMPVPREMAAALYRLVQEALTNVSKHSEATQVTIELTEQEMELSLNISDNGKGFLPEKNTTGFGLQGMQERTVALGGLF
ELWSQPQTGCHIQIKIPRLGEE
>Mature_421_residues
SRLFRSNHPFHLLLYLEWILLGVALLTLFVPFPRHHFLLPHFTPRGALRFPLGIFLSILALAGLGLKLPIHQSQKVKVLY
TCLGFLLCWATVFMGGRFRDFFTPMLLIVVIRACLLFPCRQRLIAASVAYGSFLVRLMTGAQRMRPFPRLGPPPPWRSRG
LPQELLASLQIRSALLFGLVLVFVFLMVGALLTERQSRTELAAANQRLRQYALMIENQATLQERNRIAREMHDSVGHSLT
AQSIQLENVDVWMASDRAKATDHLAKARSLGKEALQNVRHTVATLRNNPLQGQSLSQALSHLTQEFERTTSIQVQFSTHL
EMPVPREMAAALYRLVQEALTNVSKHSEATQVTIELTEQEMELSLNISDNGKGFLPEKNTTGFGLQGMQERTVALGGLFE
LWSQPQTGCHIQIKIPRLGEE

Specific function: Member of the two-component regulatory system yfiJ/yfiK. Probably activates yfiK by phosphorylation [H]

COG id: COG4585

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI87082311, Length=230, Percent_Identity=31.304347826087, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1788812, Length=191, Percent_Identity=29.8429319371728, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1787474, Length=202, Percent_Identity=27.2277227722772, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011712 [H]

Pfam domain/function: PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 47587; Mature: 47455

Theoretical pI: Translated: 10.48; Mature: 10.48

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CC
>Mature Secondary Structure 
SRLFRSNHPFHLLLYLEWILLGVALLTLFVPFPRHHFLLPHFTPRGALRFPLGIFLSIL
CCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCCHHHHHHHH
ALAGLGLKLPIHQSQKVKVLYTCLGFLLCWATVFMGGRFRDFFTPMLLIVVIRACLLFPC
HHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
RQRLIAASVAYGSFLVRLMTGAQRMRPFPRLGPPPPWRSRGLPQELLASLQIRSALLFGL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
VLVFVFLMVGALLTERQSRTELAAANQRLRQYALMIENQATLQERNRIAREMHDSVGHSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCH
TAQSIQLENVDVWMASDRAKATDHLAKARSLGKEALQNVRHTVATLRNNPLQGQSLSQAL
HHHHEEEECCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
SHLTQEFERTTSIQVQFSTHLEMPVPREMAAALYRLVQEALTNVSKHSEATQVTIELTEQ
HHHHHHHHHHHEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHEEEEEEECC
EMELSLNISDNGKGFLPEKNTTGFGLQGMQERTVALGGLFELWSQPQTGCHIQIKIPRLG
CEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCC
EE
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8973323; 9384377 [H]