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The map label for this gene is norB [H]

Identifier: 158333677

GI number: 158333677

Start: 461361

End: 463655

Strand: Direct

Name: norB [H]

Synonym: AM1_0481

Alternate gene names: 158333677

Gene position: 461361-463655 (Clockwise)

Preceding gene: 158333675

Following gene: 158333678

Centisome position: 7.09

GC content: 52.16

Gene sequence:

>2295_bases
ATGGCCAACCCAGCTTTAGATACCACTGTAGCGACGAACTTGCGATCGCGTCGCCCCTCTATGGGCTTACCCGCATGGCT
AGCCCTGATTTGTATCGTCACCTTTACCGTTCTACTCACGGCGGGGGCAGCCATTTATAAAAATGCACCGCCCATCCCCG
CTCAGATCGTGTCGCCTCAACAGGAAACGATCCTCACTCAGGCGGAGATCCAAACGGGTCAAGAAACCTACCTGGCCCGA
GGTGGCCAGCATATTGGCAGCATCTGGGGACATGGCAGCTATCTAGCTCCAGACTGGACCGCAGATGTCCTCCATCGCTG
GGGATTAGCCACTGCGGGTGTCCTGTATGACGGTGATCCTGACTTCAGTCAAGCGGATTTTGAGCAATTATCAGAGGTTG
ACCGCGCCACCCTGATCGTCCAAGTCCAGCAGGATTTTAAGGCCAATCGTTACGATGCTCACTCGGACACCTTGGCCCTC
ACTTCAGCCCAAACCCAAGGACTGCAAAGGGTCTTTGAAGACTATCAAACCTTGCTCTCCCAAGGATCTTCGATCCACTC
CATTCCTAGTGGCTGGTTTCCTGATGAGGGACAAATCCACTCGGTTACTGCCTTTTTTAGCTGGACTGCCTGGGCAGCCT
CAGCCAACCGCCCCAATGCTCCCTTCTCTTACACCGCAAACTTTCCCCACGATGATCTGATTGGTAATCAAGCGCCTGGA
CAATTTCTGATCTGGTCCATTGTGTCAGTGGTGGTCTTAATTGCGGCCATTGCCTTTTTTCTGTTCGTCTACATCATCCA
AGAAGATGCAGAAGACGTGCAGCCCGTTGCCATCCGACCCGACATTCGCCTCGCCACCCCCAGCCAAAAAGTGACAACCC
TCTTTTTTGGGGTGGCCATGATCCTATTTTTAGTCCAAATCTTGATGGGCATGGTAACCGCCCACTATGCCGTCGAAGGC
GATGGTTTTTACGGGGTCCCGATTCAACAATTTATGCCCTATGCCGCCTCCCGAACTTGGCACCTACAGATTGCGGTATT
TTGGATTGCCACTTGCTGGTTAGCGGCAGGGCTTTACTTTGGCCCTCGCTTTGGTAAGCATGAACCTAAGTTTCAATCCT
GGGGCAATGGCGGACTATTGGCCGCTCTGACCGTGGTTGTCGTCGGTTCTCTGTTAGGCACTTGGGCCGGTGTCCAGGGC
TGGCTAGGAGATAAAAGTTTCTGGTTCGGTCACCAAGGCTATGAGTATGTAGAGCTGGGCCGTCTTTGGCAGTTGCTGTT
AATTGGTGGCATGGTGTTTTGGCTATGGCTGATGTTCCGAGCCCTGAAACCTGCCCTTAAGGCGGAAGGCAACAAAACGG
GACTCAATCATTTCTTCCTGTACAGCGCCATTACCATTCCCTTGTTCTATGCGTCCGGCTTGATGTATTCCAACCATACG
CCCTTGAGCATTGCCGAATATTGGCGTTGGTGGGTGGTCCATCTTTGGGTTGAGGGATTCTTTGAAGTGTTTGCCACGGT
GGCGATTGCCTACCTTTGCAGCGAACTGGGCTTTCTGAAACGATCGACTGCACTGAAAGCCACCTACCTCACCACGATTC
TCTATCTAGGGAGTGGTGTGATTGGCACACTCCACCATCTCTACTTTGCGGGCACCCCGGTCTTTATTACGGCGATGGGG
TCTGTAGCCTCGGCTCTAGAGGTGGTCCCTCTTACCCTCATCGGGTTTGAAGTCGTTAAGTCCCTGAAGCTCTCTAAAGA
GGCTCAAGGCTTTTATCGGCTACCCCTTAAGTTTTTTATCGCCACTTGTTTCTGGAATCTAGTGGGTGCTGGTGTGTTTG
GCTTCCTGATCAATCCACCGATTGTTCTGTACTATTCCCAAGGTCTAAATACAACTCCCATTCATGCCCACTCCGCCCTG
TATGGCGTATATGGCTCCCTCGCCATCGCCCTAATGCTATTTGCCTTACGGGAAATCACGCCGGATCATGCCTGGGATGA
GAAAAATTTCAACTTCTCCTTTTGGTGGATTAACGGGGGCTTAGTGATCATGATGGTATTTGGCTTGATTCCCAACGGCT
TCTATCAACTGGTGCAGTCCATCAACCATGGTACTTGGTATGCCCGCAGCGCAGAGGTCATCAATTCCGCCTGGATGCAC
TGGACCGTTTGGCTGAGAATTCCTGGCGATATGGTCTTTGCTGTGGGGGCTTTGGTACTGGTGATGAGCGTTGGCCGAGC
TGTGATTGCCATTTGGCAACAGCCCACTCAAGCACAACCTTTAAGAGATCCCTAG

Upstream 100 bases:

>100_bases
GACCCGCTCAGCATTGGGCGAACCTTGTCCTTAGGGTTCGACTCCTCCCCACTCAAAAGATGGGCTGTTAGGAGCAAAAC
CATTTTTGGGGATAGCAACG

Downstream 100 bases:

>100_bases
AAACCTGAAAAACTCAACCCAATAAACATGAGTTGAATCACACTTAAACTCAATCGAAGCAGGAGCAATATGCCAACTTT
ATTTGACAAACTTGGTGGTT

Product: cytochrome b subunit of nitric oxide reductase

Products: NA

Alternate protein names: NOR large subunit; Nitric oxide reductase cytochrome b subunit [H]

Number of amino acids: Translated: 764; Mature: 763

Protein sequence:

>764_residues
MANPALDTTVATNLRSRRPSMGLPAWLALICIVTFTVLLTAGAAIYKNAPPIPAQIVSPQQETILTQAEIQTGQETYLAR
GGQHIGSIWGHGSYLAPDWTADVLHRWGLATAGVLYDGDPDFSQADFEQLSEVDRATLIVQVQQDFKANRYDAHSDTLAL
TSAQTQGLQRVFEDYQTLLSQGSSIHSIPSGWFPDEGQIHSVTAFFSWTAWAASANRPNAPFSYTANFPHDDLIGNQAPG
QFLIWSIVSVVVLIAAIAFFLFVYIIQEDAEDVQPVAIRPDIRLATPSQKVTTLFFGVAMILFLVQILMGMVTAHYAVEG
DGFYGVPIQQFMPYAASRTWHLQIAVFWIATCWLAAGLYFGPRFGKHEPKFQSWGNGGLLAALTVVVVGSLLGTWAGVQG
WLGDKSFWFGHQGYEYVELGRLWQLLLIGGMVFWLWLMFRALKPALKAEGNKTGLNHFFLYSAITIPLFYASGLMYSNHT
PLSIAEYWRWWVVHLWVEGFFEVFATVAIAYLCSELGFLKRSTALKATYLTTILYLGSGVIGTLHHLYFAGTPVFITAMG
SVASALEVVPLTLIGFEVVKSLKLSKEAQGFYRLPLKFFIATCFWNLVGAGVFGFLINPPIVLYYSQGLNTTPIHAHSAL
YGVYGSLAIALMLFALREITPDHAWDEKNFNFSFWWINGGLVIMMVFGLIPNGFYQLVQSINHGTWYARSAEVINSAWMH
WTVWLRIPGDMVFAVGALVLVMSVGRAVIAIWQQPTQAQPLRDP

Sequences:

>Translated_764_residues
MANPALDTTVATNLRSRRPSMGLPAWLALICIVTFTVLLTAGAAIYKNAPPIPAQIVSPQQETILTQAEIQTGQETYLAR
GGQHIGSIWGHGSYLAPDWTADVLHRWGLATAGVLYDGDPDFSQADFEQLSEVDRATLIVQVQQDFKANRYDAHSDTLAL
TSAQTQGLQRVFEDYQTLLSQGSSIHSIPSGWFPDEGQIHSVTAFFSWTAWAASANRPNAPFSYTANFPHDDLIGNQAPG
QFLIWSIVSVVVLIAAIAFFLFVYIIQEDAEDVQPVAIRPDIRLATPSQKVTTLFFGVAMILFLVQILMGMVTAHYAVEG
DGFYGVPIQQFMPYAASRTWHLQIAVFWIATCWLAAGLYFGPRFGKHEPKFQSWGNGGLLAALTVVVVGSLLGTWAGVQG
WLGDKSFWFGHQGYEYVELGRLWQLLLIGGMVFWLWLMFRALKPALKAEGNKTGLNHFFLYSAITIPLFYASGLMYSNHT
PLSIAEYWRWWVVHLWVEGFFEVFATVAIAYLCSELGFLKRSTALKATYLTTILYLGSGVIGTLHHLYFAGTPVFITAMG
SVASALEVVPLTLIGFEVVKSLKLSKEAQGFYRLPLKFFIATCFWNLVGAGVFGFLINPPIVLYYSQGLNTTPIHAHSAL
YGVYGSLAIALMLFALREITPDHAWDEKNFNFSFWWINGGLVIMMVFGLIPNGFYQLVQSINHGTWYARSAEVINSAWMH
WTVWLRIPGDMVFAVGALVLVMSVGRAVIAIWQQPTQAQPLRDP
>Mature_763_residues
ANPALDTTVATNLRSRRPSMGLPAWLALICIVTFTVLLTAGAAIYKNAPPIPAQIVSPQQETILTQAEIQTGQETYLARG
GQHIGSIWGHGSYLAPDWTADVLHRWGLATAGVLYDGDPDFSQADFEQLSEVDRATLIVQVQQDFKANRYDAHSDTLALT
SAQTQGLQRVFEDYQTLLSQGSSIHSIPSGWFPDEGQIHSVTAFFSWTAWAASANRPNAPFSYTANFPHDDLIGNQAPGQ
FLIWSIVSVVVLIAAIAFFLFVYIIQEDAEDVQPVAIRPDIRLATPSQKVTTLFFGVAMILFLVQILMGMVTAHYAVEGD
GFYGVPIQQFMPYAASRTWHLQIAVFWIATCWLAAGLYFGPRFGKHEPKFQSWGNGGLLAALTVVVVGSLLGTWAGVQGW
LGDKSFWFGHQGYEYVELGRLWQLLLIGGMVFWLWLMFRALKPALKAEGNKTGLNHFFLYSAITIPLFYASGLMYSNHTP
LSIAEYWRWWVVHLWVEGFFEVFATVAIAYLCSELGFLKRSTALKATYLTTILYLGSGVIGTLHHLYFAGTPVFITAMGS
VASALEVVPLTLIGFEVVKSLKLSKEAQGFYRLPLKFFIATCFWNLVGAGVFGFLINPPIVLYYSQGLNTTPIHAHSALY
GVYGSLAIALMLFALREITPDHAWDEKNFNFSFWWINGGLVIMMVFGLIPNGFYQLVQSINHGTWYARSAEVINSAWMHW
TVWLRIPGDMVFAVGALVLVMSVGRAVIAIWQQPTQAQPLRDP

Specific function: Component of the anaerobic respiratory chain that transforms nitrate to dinitrogen (denitrification). NorB is the catalytic subunit of the enzyme complex. Shows proton pump activity across the membrane in denitrifying bacterial cells. The mononitrogen red

COG id: COG3256

COG function: function code P; Nitric oxide reductase large subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the heme-copper respiratory oxidase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000883 [H]

Pfam domain/function: PF00115 COX1 [H]

EC number: =1.7.99.7 [H]

Molecular weight: Translated: 84811; Mature: 84680

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS00077 COX1_CUB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANPALDTTVATNLRSRRPSMGLPAWLALICIVTFTVLLTAGAAIYKNAPPIPAQIVSPQ
CCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCHHHCCCH
QETILTQAEIQTGQETYLARGGQHIGSIWGHGSYLAPDWTADVLHRWGLATAGVLYDGDP
HHHHHHHHHHHCCHHHHHHCCCCHHHHHHCCCCCCCCCHHHHHHHHCCCHHCCEEECCCC
DFSQADFEQLSEVDRATLIVQVQQDFKANRYDAHSDTLALTSAQTQGLQRVFEDYQTLLS
CCCHHHHHHHHHHHHHEEEEEEHHHCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHH
QGSSIHSIPSGWFPDEGQIHSVTAFFSWTAWAASANRPNAPFSYTANFPHDDLIGNQAPG
CCCCCCCCCCCCCCCCCCEEEEEEEHHHHHHHCCCCCCCCCCEEECCCCCHHCCCCCCCC
QFLIWSIVSVVVLIAAIAFFLFVYIIQEDAEDVQPVAIRPDIRLATPSQKVTTLFFGVAM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCEECCCCHHHHHHHHHHHH
ILFLVQILMGMVTAHYAVEGDGFYGVPIQQFMPYAASRTWHLQIAVFWIATCWLAAGLYF
HHHHHHHHHHHHHHHHEECCCCEECCCHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHC
GPRFGKHEPKFQSWGNGGLLAALTVVVVGSLLGTWAGVQGWLGDKSFWFGHQGYEYVELG
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCHHHHHH
RLWQLLLIGGMVFWLWLMFRALKPALKAEGNKTGLNHFFLYSAITIPLFYASGLMYSNHT
HHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCEECCCC
PLSIAEYWRWWVVHLWVEGFFEVFATVAIAYLCSELGFLKRSTALKATYLTTILYLGSGV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
IGTLHHLYFAGTPVFITAMGSVASALEVVPLTLIGFEVVKSLKLSKEAQGFYRLPLKFFI
HHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
ATCFWNLVGAGVFGFLINPPIVLYYSQGLNTTPIHAHSALYGVYGSLAIALMLFALREIT
HHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
PDHAWDEKNFNFSFWWINGGLVIMMVFGLIPNGFYQLVQSINHGTWYARSAEVINSAWMH
CCCCCCCCCCCEEEEEECCCHHHHHHHHHCCHHHHHHHHHCCCCCEEHHHHHHHHHHHEE
WTVWLRIPGDMVFAVGALVLVMSVGRAVIAIWQQPTQAQPLRDP
EEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
ANPALDTTVATNLRSRRPSMGLPAWLALICIVTFTVLLTAGAAIYKNAPPIPAQIVSPQ
CCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCHHHCCCH
QETILTQAEIQTGQETYLARGGQHIGSIWGHGSYLAPDWTADVLHRWGLATAGVLYDGDP
HHHHHHHHHHHCCHHHHHHCCCCHHHHHHCCCCCCCCCHHHHHHHHCCCHHCCEEECCCC
DFSQADFEQLSEVDRATLIVQVQQDFKANRYDAHSDTLALTSAQTQGLQRVFEDYQTLLS
CCCHHHHHHHHHHHHHEEEEEEHHHCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHH
QGSSIHSIPSGWFPDEGQIHSVTAFFSWTAWAASANRPNAPFSYTANFPHDDLIGNQAPG
CCCCCCCCCCCCCCCCCCEEEEEEEHHHHHHHCCCCCCCCCCEEECCCCCHHCCCCCCCC
QFLIWSIVSVVVLIAAIAFFLFVYIIQEDAEDVQPVAIRPDIRLATPSQKVTTLFFGVAM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCEECCCCHHHHHHHHHHHH
ILFLVQILMGMVTAHYAVEGDGFYGVPIQQFMPYAASRTWHLQIAVFWIATCWLAAGLYF
HHHHHHHHHHHHHHHHEECCCCEECCCHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHC
GPRFGKHEPKFQSWGNGGLLAALTVVVVGSLLGTWAGVQGWLGDKSFWFGHQGYEYVELG
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCHHHHHH
RLWQLLLIGGMVFWLWLMFRALKPALKAEGNKTGLNHFFLYSAITIPLFYASGLMYSNHT
HHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCEECCCC
PLSIAEYWRWWVVHLWVEGFFEVFATVAIAYLCSELGFLKRSTALKATYLTTILYLGSGV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
IGTLHHLYFAGTPVFITAMGSVASALEVVPLTLIGFEVVKSLKLSKEAQGFYRLPLKFFI
HHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
ATCFWNLVGAGVFGFLINPPIVLYYSQGLNTTPIHAHSALYGVYGSLAIALMLFALREIT
HHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
PDHAWDEKNFNFSFWWINGGLVIMMVFGLIPNGFYQLVQSINHGTWYARSAEVINSAWMH
CCCCCCCCCCCEEEEEECCCHHHHHHHHHCCHHHHHHHHHCCCCCEEHHHHHHHHHHHEE
WTVWLRIPGDMVFAVGALVLVMSVGRAVIAIWQQPTQAQPLRDP
EEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7711073; 10984043 [H]