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The map label for this gene is grpE [H]

Identifier: 158333620

GI number: 158333620

Start: 395595

End: 396383

Strand: Direct

Name: grpE [H]

Synonym: AM1_0421

Alternate gene names: 158333620

Gene position: 395595-396383 (Clockwise)

Preceding gene: 158333613

Following gene: 158333621

Centisome position: 6.08

GC content: 44.74

Gene sequence:

>789_bases
ATGAGCGAAGACGCGAAGCAGCACGAGAATACTGAGGCTAATGATGTAAATTACTCAGATGAATCTTCGCTTCATCAGGA
AGAGTCAAACTCAGAGTACGAAGAATTAGTGGTAGACGACTCTGAAGAGTTGCCTTTAGATGCTTTAGAAGAACTAGTTT
CATCTGCTTCGTCTGATGACGCCCCCCATACCTCGACTGCCTCACCGGAAGAAACATCTATACCTGAGCAAGAAGACTCA
TCAGAAGCGCTATCTCAGTTAGCAACGGAAGTTGAGACCTTAAAAAGTCAGTTAGACGAGCGCACGAGTCAGTATGTCCG
AATCGCAGCGGATTTCGAGAACTTTCGCAGACGCACGGCGCGAGAGAAAACAGATTTAGAACAACGGGTAAAACGAGAAA
CGCTCAGTGAGCTTCTTCCCGTTATTGATAGCTTTGATCGGGCCAGATCTCATATCAAACCTCAAACCGATCAAGAAGAG
AACATTCATAATAGTTATCAAGGGGTATACAAACAATTAGTGGATTGTTTGAAGCGAATTGGAGTTGCACCCATGCGTTC
TAAAGGACAACCCTTTGACCCGAATCTCCACGAAGCTGTAATGCGGGAACCCACTAACGAATTCGAAGAAGGTATGGTTG
TTGAAGAATTAGTCAGTGGATACTTATTAGGTGAACAGGTTCTACGGCATGCCATGGTAAAAGTTGCTGCTCCTTTAGAG
CAAGAAGAGCCAGAAGCAGTTACTGAGGAAGAAGTAGCTGAGGAAGCCGAAAATGCTGTCTCTGAATAA

Upstream 100 bases:

>100_bases
AATTACACTGAAACATGTTCGTTAAAGCTCACAACACATTAAGGTTGTAGAACTGTTAACCAAACTGTTTGTTCATGGAA
GTAAAGAAAACAGGAGCATC

Downstream 100 bases:

>100_bases
CTTTGCCAGGGTCTGAATCGTTCCCTTCTCACCTGTTTTTTGGGTTAATTTGCAGAAGGTTGATTAAATTCATGGGCAGA
TGAGGCACCCTACAGTATGA

Product: co-chaperone GrpE

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 262; Mature: 261

Protein sequence:

>262_residues
MSEDAKQHENTEANDVNYSDESSLHQEESNSEYEELVVDDSEELPLDALEELVSSASSDDAPHTSTASPEETSIPEQEDS
SEALSQLATEVETLKSQLDERTSQYVRIAADFENFRRRTAREKTDLEQRVKRETLSELLPVIDSFDRARSHIKPQTDQEE
NIHNSYQGVYKQLVDCLKRIGVAPMRSKGQPFDPNLHEAVMREPTNEFEEGMVVEELVSGYLLGEQVLRHAMVKVAAPLE
QEEPEAVTEEEVAEEAENAVSE

Sequences:

>Translated_262_residues
MSEDAKQHENTEANDVNYSDESSLHQEESNSEYEELVVDDSEELPLDALEELVSSASSDDAPHTSTASPEETSIPEQEDS
SEALSQLATEVETLKSQLDERTSQYVRIAADFENFRRRTAREKTDLEQRVKRETLSELLPVIDSFDRARSHIKPQTDQEE
NIHNSYQGVYKQLVDCLKRIGVAPMRSKGQPFDPNLHEAVMREPTNEFEEGMVVEELVSGYLLGEQVLRHAMVKVAAPLE
QEEPEAVTEEEVAEEAENAVSE
>Mature_261_residues
SEDAKQHENTEANDVNYSDESSLHQEESNSEYEELVVDDSEELPLDALEELVSSASSDDAPHTSTASPEETSIPEQEDSS
EALSQLATEVETLKSQLDERTSQYVRIAADFENFRRRTAREKTDLEQRVKRETLSELLPVIDSFDRARSHIKPQTDQEEN
IHNSYQGVYKQLVDCLKRIGVAPMRSKGQPFDPNLHEAVMREPTNEFEEGMVVEELVSGYLLGEQVLRHAMVKVAAPLEQ
EEPEAVTEEEVAEEAENAVSE

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:
GO:0003824: Ribulose-phosphate 3-epimerase
GO:0004750: Ribulose-phosphate 3-epimerase
GO:0005975: Ribulose-phosphate 3-epimerase
GO:0008152: Ribulose-phosphate 3-epimerase
GO:0016853: Ribulose-phosphate 3-epimerase

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Escherichia coli, GI1788967, Length=171, Percent_Identity=29.8245614035088, Blast_Score=74, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24653432, Length=178, Percent_Identity=29.7752808988764, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 29581; Mature: 29450

Theoretical pI: Translated: 4.00; Mature: 4.00

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEDAKQHENTEANDVNYSDESSLHQEESNSEYEELVVDDSEELPLDALEELVSSASSDD
CCCHHHHHCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCC
APHTSTASPEETSIPEQEDSSEALSQLATEVETLKSQLDERTSQYVRIAADFENFRRRTA
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
REKTDLEQRVKRETLSELLPVIDSFDRARSHIKPQTDQEENIHNSYQGVYKQLVDCLKRI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
GVAPMRSKGQPFDPNLHEAVMREPTNEFEEGMVVEELVSGYLLGEQVLRHAMVKVAAPLE
CCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
QEEPEAVTEEEVAEEAENAVSE
CCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SEDAKQHENTEANDVNYSDESSLHQEESNSEYEELVVDDSEELPLDALEELVSSASSDD
CCHHHHHCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCC
APHTSTASPEETSIPEQEDSSEALSQLATEVETLKSQLDERTSQYVRIAADFENFRRRTA
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
REKTDLEQRVKRETLSELLPVIDSFDRARSHIKPQTDQEENIHNSYQGVYKQLVDCLKRI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
GVAPMRSKGQPFDPNLHEAVMREPTNEFEEGMVVEELVSGYLLGEQVLRHAMVKVAAPLE
CCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
QEEPEAVTEEEVAEEAENAVSE
CCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA