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The map label for this gene is nucA [H]

Identifier: 158333610

GI number: 158333610

Start: 384928

End: 385743

Strand: Direct

Name: nucA [H]

Synonym: AM1_0411

Alternate gene names: 158333610

Gene position: 384928-385743 (Clockwise)

Preceding gene: 158333609

Following gene: 158333611

Centisome position: 5.92

GC content: 45.34

Gene sequence:

>816_bases
ATGCGATCGCATCTACCGATCACGATTTTTCTATGTTTTTCAATCTTGACCGGATGTAGTTTCTTCTCAACTTTCCCCCA
ATCAAAGGTTGACCACTTAGCTCTCGGGAATCCTAGTCAAGCCAGAGTCGTCTTGGCCAACAGTAATAATTATTTGATGG
AAAAGCCTCAATTTGCCCTGTCTTACAACCGCAGCAAGGGAATTCCCAACTGGGTGAGTTGGCAGCTTGATCAAACTTGG
TTAGGAGACGTCGAACGTCGCAATGATTTTCGAGCGGATCAAGGCTTGCCGCCAAAATGGGAGAAAGTTGACTCAAGAGA
CTATACCCGGAGTGGCTATGATCGAGGGCATATGGCTCCTTCTGGTGATCGAACTAATTCAGAAGTCAACAATTCTGCAA
CCTTCGTCATGACCAATATTGTGCCCCAGCGGCCAGATAACAACCGAGGGCCATGGGTTGACCTGGAGAATTACTGTCGA
GATTTAGTGGATGAAGGCAAAGAGCTATTCATTATTGCGGGAGGATATGGGAAACGAGCTGCGATCGCAAAAGGAAAAGT
CACACCTCCCCAAAGCCTCTGGAAAATCATCGTCGTAATGGATGAGACAACATTAGGTGTAAATGGTATTTCTACCAATA
CTCCAGTAATTGCCGTTGATATTCCCAATAAGCAGGGGATTAAATCCCATGAATGGCAACGGTACATTGTCACCGTCGAC
CATTTAGAACATGAAACGGGGTATGACTTCTTGTCTAATATCCCCGACCCCCTTCAAGCTAGATTAGAAAGCCAAAAAGC
TGTTTTAAGCACATGA

Upstream 100 bases:

>100_bases
CCAGATGCAACCGTTGCTTTCAATTATGGATTGAAAAATAGTGATACGGCTTTTCAGATTACACCCGGTTGGTTGGGCAA
GGAAGATTGCGGGCTTGATG

Downstream 100 bases:

>100_bases
AAGAGCAGGCTGCAAAAGTCATGCTCAGCACTTCCTCATAATCTGTACCCCACACTCCACGCCCACTCTAGACACGGCAA
TAGAGACATCTGAATATGAT

Product: DNA/RNA non-specific endonuclease

Products: NA

Alternate protein names: Endonuclease [H]

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MRSHLPITIFLCFSILTGCSFFSTFPQSKVDHLALGNPSQARVVLANSNNYLMEKPQFALSYNRSKGIPNWVSWQLDQTW
LGDVERRNDFRADQGLPPKWEKVDSRDYTRSGYDRGHMAPSGDRTNSEVNNSATFVMTNIVPQRPDNNRGPWVDLENYCR
DLVDEGKELFIIAGGYGKRAAIAKGKVTPPQSLWKIIVVMDETTLGVNGISTNTPVIAVDIPNKQGIKSHEWQRYIVTVD
HLEHETGYDFLSNIPDPLQARLESQKAVLST

Sequences:

>Translated_271_residues
MRSHLPITIFLCFSILTGCSFFSTFPQSKVDHLALGNPSQARVVLANSNNYLMEKPQFALSYNRSKGIPNWVSWQLDQTW
LGDVERRNDFRADQGLPPKWEKVDSRDYTRSGYDRGHMAPSGDRTNSEVNNSATFVMTNIVPQRPDNNRGPWVDLENYCR
DLVDEGKELFIIAGGYGKRAAIAKGKVTPPQSLWKIIVVMDETTLGVNGISTNTPVIAVDIPNKQGIKSHEWQRYIVTVD
HLEHETGYDFLSNIPDPLQARLESQKAVLST
>Mature_271_residues
MRSHLPITIFLCFSILTGCSFFSTFPQSKVDHLALGNPSQARVVLANSNNYLMEKPQFALSYNRSKGIPNWVSWQLDQTW
LGDVERRNDFRADQGLPPKWEKVDSRDYTRSGYDRGHMAPSGDRTNSEVNNSATFVMTNIVPQRPDNNRGPWVDLENYCR
DLVDEGKELFIIAGGYGKRAAIAKGKVTPPQSLWKIIVVMDETTLGVNGISTNTPVIAVDIPNKQGIKSHEWQRYIVTVD
HLEHETGYDFLSNIPDPLQARLESQKAVLST

Specific function: Catalyzes the degradation of both RNA and DNA; has the potential to act as an endonuclease [H]

COG id: COG1864

COG function: function code F; DNA/RNA endonuclease G, NUC1

Gene ontology:

Cell location: Periplasm. Note=Periplasmic or loosely attached to the cytoplasmic or the outer membrane [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA/RNA non-specific endonuclease family [H]

Homologues:

Organism=Homo sapiens, GI224451073, Length=211, Percent_Identity=30.3317535545024, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI224451075, Length=187, Percent_Identity=29.9465240641711, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI53759134, Length=232, Percent_Identity=28.448275862069, Blast_Score=81, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6322253, Length=222, Percent_Identity=29.7297297297297, Blast_Score=87, Evalue=2e-18,
Organism=Drosophila melanogaster, GI20129899, Length=198, Percent_Identity=27.2727272727273, Blast_Score=78, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24581065, Length=217, Percent_Identity=29.0322580645161, Blast_Score=78, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018524
- InterPro:   IPR001604
- InterPro:   IPR020821 [H]

Pfam domain/function: PF01223 Endonuclease_NS [H]

EC number: NA

Molecular weight: Translated: 30554; Mature: 30554

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01070 NUCLEASE_NON_SPEC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSHLPITIFLCFSILTGCSFFSTFPQSKVDHLALGNPSQARVVLANSNNYLMEKPQFAL
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCEEEEEECCCCEEEECCCEEE
SYNRSKGIPNWVSWQLDQTWLGDVERRNDFRADQGLPPKWEKVDSRDYTRSGYDRGHMAP
EECCCCCCCCCEEEEECCHHHCCHHHHCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCC
SGDRTNSEVNNSATFVMTNIVPQRPDNNRGPWVDLENYCRDLVDEGKELFIIAGGYGKRA
CCCCCCCCCCCCEEEEEEECCCCCCCCCCCCEECHHHHHHHHHHCCCEEEEEECCCCCEE
AIAKGKVTPPQSLWKIIVVMDETTLGVNGISTNTPVIAVDIPNKQGIKSHEWQRYIVTVD
EECCCCCCCHHHHEEEEEEEECCEECCCCCCCCCCEEEEECCCCCCCCCCCCEEEEEEEE
HLEHETGYDFLSNIPDPLQARLESQKAVLST
HHCCCCCCHHHHCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRSHLPITIFLCFSILTGCSFFSTFPQSKVDHLALGNPSQARVVLANSNNYLMEKPQFAL
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCEEEEEECCCCEEEECCCEEE
SYNRSKGIPNWVSWQLDQTWLGDVERRNDFRADQGLPPKWEKVDSRDYTRSGYDRGHMAP
EECCCCCCCCCEEEEECCHHHCCHHHHCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCC
SGDRTNSEVNNSATFVMTNIVPQRPDNNRGPWVDLENYCRDLVDEGKELFIIAGGYGKRA
CCCCCCCCCCCCEEEEEEECCCCCCCCCCCCEECHHHHHHHHHHCCCEEEEEECCCCCEE
AIAKGKVTPPQSLWKIIVVMDETTLGVNGISTNTPVIAVDIPNKQGIKSHEWQRYIVTVD
EECCCCCCCHHHHEEEEEEEECCEECCCCCCCCCCEEEEECCCCCCCCCCCCEEEEEEEE
HLEHETGYDFLSNIPDPLQARLESQKAVLST
HHCCCCCCHHHHCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1343821; 11759840 [H]