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The map label for this gene is rfbA [H]

Identifier: 158333603

GI number: 158333603

Start: 378044

End: 378925

Strand: Reverse

Name: rfbA [H]

Synonym: AM1_0404

Alternate gene names: 158333603

Gene position: 378925-378044 (Counterclockwise)

Preceding gene: 158333604

Following gene: 158333602

Centisome position: 5.83

GC content: 44.44

Gene sequence:

>882_bases
ATGAAAGGAATTATTCTGGCTGGCGGTTCAGGTACTAGGCTTTATCCCATTACTCGGGTGCTATCTAAACAGCTAATGCC
TGTATACGATAAGCCCATGATTTACTATCCCCTCTCAACCTTAATGTTGGCGGGGATCCGGGAAATTCTGATTATCTCTA
CCCCTCAAGATTTACCGTTGTTTCAGCGATTATTTGGAGATGGTCATCAGCTGGGATTAACCTTTTCCTATGCAGAACAA
CCTAAACCTGAAGGTTTGGCCCAAGCTTTTATTATTGGTCAGGACTTTATTGGTGATGATTCGGTTGCTCTGATTCTTGG
AGATAATATTTTCTATGGATCGGCACTAGCAGAGCGACTTCAGCAAATATCGCGACTCACTGAGGGGGGGATTGTGTTTG
GCTATTGGGTCAAGGATCCACAGCGGTACGGGGTGTTAGCCTTCGATCAACAAGGTCAGGTTACGGATATTGAGGAAAAA
CCCCAGCACCCAAAATCTCATTACGCAGTGACGGGGCTTTATTTTTATGACAACCAAGTGGTTGATATTGCTAAAACTTT
GAAGCCTTCTCCTAGAGGGGAATTAGAAATAACGGATGTCAATACAACATATCTCAAACAAGGACAATTAAAAGTTGAGA
AAATGAGTCGTGGGGTGGCGTGGCTAGATACGGGCACCCATGAAAGTTTGCTGCAAGCGGCTGCTTTTGTGGAGACGATT
GAACAACGACAAGGATTGAAGATTGCCTGTGTGGAAGAGGTGGCTTATCGAATGGGATATATCTCAGCTGCTCAAGTTAA
AGAATTAAGTCGAGATTTGTGCAAGAACAGTTATGGAGAGTATCTCCTGAGAGTGCTGGAAGAGGGAGGTCAACCCCTGT
GA

Upstream 100 bases:

>100_bases
CTCAGCAGTAAGAGAGCTTTGCTCATGTTATACATCTCCAGTTAGATGGGCATAGTTTGAAGAAAGAACGCTGCAGTTAT
AGATTGCCGAGATAATACTG

Downstream 100 bases:

>100_bases
ATGTGATAGAAACTCCTTTGCCGGGTGTTTTAGTGATTGAACCTCGTGTATTTTCGGACCAACGGGGCTTTTTCTTGGAA
ACTTATCATCGCCAACGGTA

Product: glucose-1-phosphate thymidylyltransferase

Products: NA

Alternate protein names: dTDP-glucose pyrophosphorylase; dTDP-glucose synthase [H]

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MKGIILAGGSGTRLYPITRVLSKQLMPVYDKPMIYYPLSTLMLAGIREILIISTPQDLPLFQRLFGDGHQLGLTFSYAEQ
PKPEGLAQAFIIGQDFIGDDSVALILGDNIFYGSALAERLQQISRLTEGGIVFGYWVKDPQRYGVLAFDQQGQVTDIEEK
PQHPKSHYAVTGLYFYDNQVVDIAKTLKPSPRGELEITDVNTTYLKQGQLKVEKMSRGVAWLDTGTHESLLQAAAFVETI
EQRQGLKIACVEEVAYRMGYISAAQVKELSRDLCKNSYGEYLLRVLEEGGQPL

Sequences:

>Translated_293_residues
MKGIILAGGSGTRLYPITRVLSKQLMPVYDKPMIYYPLSTLMLAGIREILIISTPQDLPLFQRLFGDGHQLGLTFSYAEQ
PKPEGLAQAFIIGQDFIGDDSVALILGDNIFYGSALAERLQQISRLTEGGIVFGYWVKDPQRYGVLAFDQQGQVTDIEEK
PQHPKSHYAVTGLYFYDNQVVDIAKTLKPSPRGELEITDVNTTYLKQGQLKVEKMSRGVAWLDTGTHESLLQAAAFVETI
EQRQGLKIACVEEVAYRMGYISAAQVKELSRDLCKNSYGEYLLRVLEEGGQPL
>Mature_293_residues
MKGIILAGGSGTRLYPITRVLSKQLMPVYDKPMIYYPLSTLMLAGIREILIISTPQDLPLFQRLFGDGHQLGLTFSYAEQ
PKPEGLAQAFIIGQDFIGDDSVALILGDNIFYGSALAERLQQISRLTEGGIVFGYWVKDPQRYGVLAFDQQGQVTDIEEK
PQHPKSHYAVTGLYFYDNQVVDIAKTLKPSPRGELEITDVNTTYLKQGQLKVEKMSRGVAWLDTGTHESLLQAAAFVETI
EQRQGLKIACVEEVAYRMGYISAAQVKELSRDLCKNSYGEYLLRVLEEGGQPL

Specific function: Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis [H]

COG id: COG1209

COG function: function code M; dTDP-glucose pyrophosphorylase

Gene ontology:
GO:0003824: Fructose-bisphosphate aldolase, class II, Calvin cycle subtype
GO:0004332: Fructose-bisphosphate aldolase, class II, Calvin cycle subtype
GO:0005975: Fructose-bisphosphate aldolase, class II, Calvin cycle subtype
GO:0006096: Fructose-bisphosphate aldolase, class II, Calvin cycle subtype
GO:0008152: Fructose-bisphosphate aldolase, class II, Calvin cycle subtype
GO:0008270: Fructose-bisphosphate aldolase, class II, Calvin cycle subtype
GO:0016829: Fructose-bisphosphate aldolase, class II, Calvin cycle subtype
GO:0016832: Fructose-bisphosphate aldolase, class II, Calvin cycle subtype
GO:0008879: Glucose-1-phosphate thymidylyltransferase
GO:0009058: Glucose-1-phosphate thymidylyltransferase
GO:0016740: Glucose-1-phosphate thymidylyltransferase
GO:0016779: Glucose-1-phosphate thymidylyltransferase
GO:0045226: Glucose-1-phosphate thymidylyltransferase
GO:0046872: Glucose-1-phosphate thymidylyltransferase

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucose-1-phosphate thymidylyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI11761619, Length=247, Percent_Identity=23.8866396761134, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI11761621, Length=247, Percent_Identity=23.8866396761134, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1788351, Length=287, Percent_Identity=63.4146341463415, Blast_Score=389, Evalue=1e-109,
Organism=Escherichia coli, GI1790224, Length=286, Percent_Identity=60.4895104895105, Blast_Score=373, Evalue=1e-105,
Organism=Escherichia coli, GI1788355, Length=239, Percent_Identity=24.6861924686192, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787488, Length=274, Percent_Identity=25.5474452554745, Blast_Score=64, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI133931050, Length=191, Percent_Identity=26.1780104712042, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6320148, Length=254, Percent_Identity=24.4094488188976, Blast_Score=71, Evalue=3e-13,
Organism=Drosophila melanogaster, GI21355443, Length=251, Percent_Identity=22.7091633466135, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24644084, Length=251, Percent_Identity=22.7091633466135, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005907
- InterPro:   IPR005835 [H]

Pfam domain/function: PF00483 NTP_transferase [H]

EC number: =2.7.7.24 [H]

Molecular weight: Translated: 32675; Mature: 32675

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGIILAGGSGTRLYPITRVLSKQLMPVYDKPMIYYPLSTLMLAGIREILIISTPQDLPL
CCCEEEECCCCCEEHHHHHHHHHHCCCHHCCCEEEECHHHHHHHHHHEEEEEECCCCCHH
FQRLFGDGHQLGLTFSYAEQPKPEGLAQAFIIGQDFIGDDSVALILGDNIFYGSALAERL
HHHHHCCCCEEEEEEECCCCCCCCHHHHEEEECCCCCCCCCEEEEEECCEEEHHHHHHHH
QQISRLTEGGIVFGYWVKDPQRYGVLAFDQQGQVTDIEEKPQHPKSHYAVTGLYFYDNQV
HHHHHHCCCCEEEEEEECCCCCCCEEEECCCCCEECCHHCCCCCCCCEEEEEEEEECCCH
VDIAKTLKPSPRGELEITDVNTTYLKQGQLKVEKMSRGVAWLDTGTHESLLQAAAFVETI
HHHHHHCCCCCCCCEEEEECCHHHHHCCCEEHHHHHCCEEEEECCCHHHHHHHHHHHHHH
EQRQGLKIACVEEVAYRMGYISAAQVKELSRDLCKNSYGEYLLRVLEEGGQPL
HHHCCCEEEHHHHHHHHHCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKGIILAGGSGTRLYPITRVLSKQLMPVYDKPMIYYPLSTLMLAGIREILIISTPQDLPL
CCCEEEECCCCCEEHHHHHHHHHHCCCHHCCCEEEECHHHHHHHHHHEEEEEECCCCCHH
FQRLFGDGHQLGLTFSYAEQPKPEGLAQAFIIGQDFIGDDSVALILGDNIFYGSALAERL
HHHHHCCCCEEEEEEECCCCCCCCHHHHEEEECCCCCCCCCEEEEEECCEEEHHHHHHHH
QQISRLTEGGIVFGYWVKDPQRYGVLAFDQQGQVTDIEEKPQHPKSHYAVTGLYFYDNQV
HHHHHHCCCCEEEEEEECCCCCCCEEEECCCCCEECCHHCCCCCCCCEEEEEEEEECCCH
VDIAKTLKPSPRGELEITDVNTTYLKQGQLKVEKMSRGVAWLDTGTHESLLQAAAFVETI
HHHHHHCCCCCCCCEEEEECCHHHHHCCCEEHHHHHCCEEEEECCCHHHHHHHHHHHHHH
EQRQGLKIACVEEVAYRMGYISAAQVKELSRDLCKNSYGEYLLRVLEEGGQPL
HHHCCCEEEHHHHHHHHHCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12397186 [H]