Click here to switch to the map view.

The map label for this gene is rfbB [H]

Identifier: 158333604

GI number: 158333604

Start: 379015

End: 380082

Strand: Reverse

Name: rfbB [H]

Synonym: AM1_0405

Alternate gene names: 158333604

Gene position: 380082-379015 (Counterclockwise)

Preceding gene: 158333605

Following gene: 158333603

Centisome position: 5.84

GC content: 46.07

Gene sequence:

>1068_bases
ATGACGATTCTGGTTACGGGGGGAGCCGGTTTTATTGGCGCAAACTATGTTTTGCGCAAGCGGCAGAATGATGAAGGCTT
GGTATTGAACCTAGATAAGTTGACCTATGCAGGCCACTTATCTACTTTAGATTCCCTGAAGTCTGATCCAGACCATATCT
TTATTCAAGGGGATATTGGTGATCGTCAGCTTGTTGAAGACTTGCTAACTCAGTATCGTCCTAGGGCGATTCTCAATTTT
GCAGCTGAAACCCATGTAGACCGTTCCATTGATGGCCCAGATGCCTTTATTCAAACGAATATTGTTGGCACTTTCAACCT
ATTAGAAACGGTTCGGTCCTACTGGCAGAACCTCTCTGTGGCTGAACGAAACAGCTTTCGGTTTCTGCATGTTTCGACGG
ATGAAGTCTTTGGGACCCTCTCGGCTGTAGATCCTCCGTTCTCGGAATCGACGGCCTATGCTCCTAATAGCCCTTATTCT
GCCTCCAAAGCCAGCTCCGATCACCTGGTGAGATCTTATTTCCACACTTACAATCTGCCAACGTTAATTACCAACTGTTC
CAATAACTACGGCCCTTACCAATTCCCAGAGAAGTTAATTCCTTTGATGATCTTGAATGCAATTGAGGGCAAGCCGTTGC
CTGTGTATGGAGATGGGCAAAATATTCGAGATTGGCTCTTTGTGGAAGATCACTGTCGAGCCATAGATCTGGTGTTGACT
CAAGGGGAAATGGGTGAAACCTACAATATTGGCGGGAACGCGGAAAAAACGAATCTCTGGCTCGTCCAACAAATCTGTGC
AATTTTGGATGATCTGCTTCCTCATTCAGCCCACAGACCCCATGCAAATTTAATTACTTTTGTGCAAGATCGACCGGGGC
ATGATCGACGGTACGCGATCAATTTTTCTAAAATCAAACAACACCTGGGTTGGTCTCCGCAAGTCACTATTGAGTCTGGA
TTAAGGAAAACGGTGCAGTGGTATTTAGACCATCAGGCATGGTGTGATCAGGTTACATCCGACCAGTATGAACGCCAACG
GTTGGGATTAACGGCTACTCAGCAGTAA

Upstream 100 bases:

>100_bases
ACAGCAGGTGATTGAATTTTTACAGGAGCGGGGGATTGTTCAACCCACCTCTGCTTAGCTAAGGTAAATTGACGAGTCAA
TTACTAAAAAACAAAATATC

Downstream 100 bases:

>100_bases
GAGAGCTTTGCTCATGTTATACATCTCCAGTTAGATGGGCATAGTTTGAAGAAAGAACGCTGCAGTTATAGATTGCCGAG
ATAATACTGATGAAAGGAAT

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 355; Mature: 354

Protein sequence:

>355_residues
MTILVTGGAGFIGANYVLRKRQNDEGLVLNLDKLTYAGHLSTLDSLKSDPDHIFIQGDIGDRQLVEDLLTQYRPRAILNF
AAETHVDRSIDGPDAFIQTNIVGTFNLLETVRSYWQNLSVAERNSFRFLHVSTDEVFGTLSAVDPPFSESTAYAPNSPYS
ASKASSDHLVRSYFHTYNLPTLITNCSNNYGPYQFPEKLIPLMILNAIEGKPLPVYGDGQNIRDWLFVEDHCRAIDLVLT
QGEMGETYNIGGNAEKTNLWLVQQICAILDDLLPHSAHRPHANLITFVQDRPGHDRRYAINFSKIKQHLGWSPQVTIESG
LRKTVQWYLDHQAWCDQVTSDQYERQRLGLTATQQ

Sequences:

>Translated_355_residues
MTILVTGGAGFIGANYVLRKRQNDEGLVLNLDKLTYAGHLSTLDSLKSDPDHIFIQGDIGDRQLVEDLLTQYRPRAILNF
AAETHVDRSIDGPDAFIQTNIVGTFNLLETVRSYWQNLSVAERNSFRFLHVSTDEVFGTLSAVDPPFSESTAYAPNSPYS
ASKASSDHLVRSYFHTYNLPTLITNCSNNYGPYQFPEKLIPLMILNAIEGKPLPVYGDGQNIRDWLFVEDHCRAIDLVLT
QGEMGETYNIGGNAEKTNLWLVQQICAILDDLLPHSAHRPHANLITFVQDRPGHDRRYAINFSKIKQHLGWSPQVTIESG
LRKTVQWYLDHQAWCDQVTSDQYERQRLGLTATQQ
>Mature_354_residues
TILVTGGAGFIGANYVLRKRQNDEGLVLNLDKLTYAGHLSTLDSLKSDPDHIFIQGDIGDRQLVEDLLTQYRPRAILNFA
AETHVDRSIDGPDAFIQTNIVGTFNLLETVRSYWQNLSVAERNSFRFLHVSTDEVFGTLSAVDPPFSESTAYAPNSPYSA
SKASSDHLVRSYFHTYNLPTLITNCSNNYGPYQFPEKLIPLMILNAIEGKPLPVYGDGQNIRDWLFVEDHCRAIDLVLTQ
GEMGETYNIGGNAEKTNLWLVQQICAILDDLLPHSAHRPHANLITFVQDRPGHDRRYAINFSKIKQHLGWSPQVTIESGL
RKTVQWYLDHQAWCDQVTSDQYERQRLGLTATQQ

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=330, Percent_Identity=35.1515151515151, Blast_Score=212, Evalue=4e-55,
Organism=Homo sapiens, GI42516563, Length=369, Percent_Identity=24.390243902439, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI4504031, Length=345, Percent_Identity=24.0579710144928, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI48994969, Length=350, Percent_Identity=58, Blast_Score=420, Evalue=1e-119,
Organism=Escherichia coli, GI1788353, Length=352, Percent_Identity=56.25, Blast_Score=410, Evalue=1e-116,
Organism=Escherichia coli, GI1788366, Length=294, Percent_Identity=22.4489795918367, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1786974, Length=356, Percent_Identity=22.752808988764, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17568069, Length=332, Percent_Identity=37.3493975903614, Blast_Score=218, Evalue=4e-57,
Organism=Caenorhabditis elegans, GI115532424, Length=333, Percent_Identity=31.8318318318318, Blast_Score=162, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI17539532, Length=331, Percent_Identity=25.9818731117825, Blast_Score=88, Evalue=8e-18,
Organism=Saccharomyces cerevisiae, GI6319493, Length=356, Percent_Identity=23.5955056179775, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21356223, Length=352, Percent_Identity=23.5795454545455, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI19923002, Length=361, Percent_Identity=25.207756232687, Blast_Score=75, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24158427, Length=335, Percent_Identity=22.6865671641791, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 40110; Mature: 39979

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTILVTGGAGFIGANYVLRKRQNDEGLVLNLDKLTYAGHLSTLDSLKSDPDHIFIQGDIG
CEEEEECCCCCCCCCHHEEECCCCCCEEEEEHHHEEHHHHHHHHHHCCCCCEEEEECCCC
DRQLVEDLLTQYRPRAILNFAAETHVDRSIDGPDAFIQTNIVGTFNLLETVRSYWQNLSV
CHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHCCCE
AERNSFRFLHVSTDEVFGTLSAVDPPFSESTAYAPNSPYSASKASSDHLVRSYFHTYNLP
ECCCCEEEEEEEHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCC
TLITNCSNNYGPYQFPEKLIPLMILNAIEGKPLPVYGDGQNIRDWLFVEDHCRAIDLVLT
HHEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEECCCCCCCCEEEECCCCCEEEEEEE
QGEMGETYNIGGNAEKTNLWLVQQICAILDDLLPHSAHRPHANLITFVQDRPGHDRRYAI
CCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCEEEE
NFSKIKQHLGWSPQVTIESGLRKTVQWYLDHQAWCDQVTSDQYERQRLGLTATQQ
EHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TILVTGGAGFIGANYVLRKRQNDEGLVLNLDKLTYAGHLSTLDSLKSDPDHIFIQGDIG
EEEEECCCCCCCCCHHEEECCCCCCEEEEEHHHEEHHHHHHHHHHCCCCCEEEEECCCC
DRQLVEDLLTQYRPRAILNFAAETHVDRSIDGPDAFIQTNIVGTFNLLETVRSYWQNLSV
CHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHCCCE
AERNSFRFLHVSTDEVFGTLSAVDPPFSESTAYAPNSPYSASKASSDHLVRSYFHTYNLP
ECCCCEEEEEEEHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCC
TLITNCSNNYGPYQFPEKLIPLMILNAIEGKPLPVYGDGQNIRDWLFVEDHCRAIDLVLT
HHEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEECCCCCCCCEEEECCCCCEEEEEEE
QGEMGETYNIGGNAEKTNLWLVQQICAILDDLLPHSAHRPHANLITFVQDRPGHDRRYAI
CCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCEEEE
NFSKIKQHLGWSPQVTIESGLRKTVQWYLDHQAWCDQVTSDQYERQRLGLTATQQ
EHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8253667 [H]