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The map label for this gene is rfbB [C]

Identifier: 158333592

GI number: 158333592

Start: 366086

End: 367057

Strand: Direct

Name: rfbB [C]

Synonym: AM1_0393

Alternate gene names: 158333592

Gene position: 366086-367057 (Clockwise)

Preceding gene: 158333591

Following gene: 158333593

Centisome position: 5.63

GC content: 44.75

Gene sequence:

>972_bases
ATGCGAGTTCTGGTTACAGGTGCCGCCGGTTTTATTGGTTACCATCTATCTCAACGCCTTTTGCTAGATAGAGCACAGGT
ATTTGGAATCGACAATCTCAATAACTATTATGCTGTCGATCTCAAAAAATCACGACTCGCCCAATTAGAGCCCAATCAGA
ATTTCCAGTTCCAATGTCTAGATCTCAGCGATCGCAACGGTATGGAAACCCTGTTTGAGAGCAATACCTTTGATGGTGTA
ATTCATTTAGCTGCTCAGGCTGGGGTTCGCTATTCTCTAGACAACCCTCATGCCTATGTAGACAGTAATCTGGTGGGCTT
CCTTCATATCCTTGAGGGCTGTCGCCAAAGCAACATATCTCATCTGGTTTATGCGTCCTCTAGCTCAGTCTATGGTGCCA
ATAAAAAAGTCCCTTTTTCCGTAGAAGATAATGTGGACCATCCCGTTTCTTTATATGCCGCCACCAAGAAATCGAATGAG
TTAATGGCCCATTCCTATAGCCATCTATATCAAATACCGATCACCGGACTGCGATTTTTTACGGTATATGGTCCCTGGGG
ACGGCCAGATATGGCCTATTTCAAGTTTGTGGATGCCATAGCAAATAACAAATCCATCGATGTCTACAACCACGGAAAAA
TGCAGCGAGATTTTACCTATATCGATGATGTCGTCGAAGGTATTGTCCGAGTTCTCCATCAGCCTCCAAACCCAGACACC
ACAACCCCTCCCTACAAGCTCTACAACATTGGCAATAACCAACCCGTAACCCTGATGCGCTTTATCGAAGTAATTGAAAC
CGCCATGGGTAAAACGGCGGACAAGAACTTTTTACCCATGCAACCGGGGGATGTCCCAGCAACCTATGCAGACGTAGATG
CCTTAATGAATGACGTGGGCTTTCAACCCAAAACCCCAATTGAAGATGGGATTCAAAAATTTGTAACTTGGTATCGCAGC
TATTATCAATAG

Upstream 100 bases:

>100_bases
CAGAAAACCAGCATCCATGCCTTGCCAGTATGGTTATGAGAAGAGTAATAGACCTCTCCTGTAAGGGAACTATGAAGTTA
GTGAATGGAGATAATTAACC

Downstream 100 bases:

>100_bases
CTCATTTCAAACCTGTGTTTTCTTCCATCTCTGCCTAAAACTTCAGCAAAGCTTGTGGATTGCCTTCATCAGAATCAAGG
TGGAATCCGATGTTAGCAAC

Product: NAD-dependent epimerase/dehydratase family protein

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MRVLVTGAAGFIGYHLSQRLLLDRAQVFGIDNLNNYYAVDLKKSRLAQLEPNQNFQFQCLDLSDRNGMETLFESNTFDGV
IHLAAQAGVRYSLDNPHAYVDSNLVGFLHILEGCRQSNISHLVYASSSSVYGANKKVPFSVEDNVDHPVSLYAATKKSNE
LMAHSYSHLYQIPITGLRFFTVYGPWGRPDMAYFKFVDAIANNKSIDVYNHGKMQRDFTYIDDVVEGIVRVLHQPPNPDT
TTPPYKLYNIGNNQPVTLMRFIEVIETAMGKTADKNFLPMQPGDVPATYADVDALMNDVGFQPKTPIEDGIQKFVTWYRS
YYQ

Sequences:

>Translated_323_residues
MRVLVTGAAGFIGYHLSQRLLLDRAQVFGIDNLNNYYAVDLKKSRLAQLEPNQNFQFQCLDLSDRNGMETLFESNTFDGV
IHLAAQAGVRYSLDNPHAYVDSNLVGFLHILEGCRQSNISHLVYASSSSVYGANKKVPFSVEDNVDHPVSLYAATKKSNE
LMAHSYSHLYQIPITGLRFFTVYGPWGRPDMAYFKFVDAIANNKSIDVYNHGKMQRDFTYIDDVVEGIVRVLHQPPNPDT
TTPPYKLYNIGNNQPVTLMRFIEVIETAMGKTADKNFLPMQPGDVPATYADVDALMNDVGFQPKTPIEDGIQKFVTWYRS
YYQ
>Mature_323_residues
MRVLVTGAAGFIGYHLSQRLLLDRAQVFGIDNLNNYYAVDLKKSRLAQLEPNQNFQFQCLDLSDRNGMETLFESNTFDGV
IHLAAQAGVRYSLDNPHAYVDSNLVGFLHILEGCRQSNISHLVYASSSSVYGANKKVPFSVEDNVDHPVSLYAATKKSNE
LMAHSYSHLYQIPITGLRFFTVYGPWGRPDMAYFKFVDAIANNKSIDVYNHGKMQRDFTYIDDVVEGIVRVLHQPPNPDT
TTPPYKLYNIGNNQPVTLMRFIEVIETAMGKTADKNFLPMQPGDVPATYADVDALMNDVGFQPKTPIEDGIQKFVTWYRS
YYQ

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=328, Percent_Identity=28.6585365853659, Blast_Score=139, Evalue=3e-33,
Organism=Homo sapiens, GI42516563, Length=329, Percent_Identity=26.7477203647416, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI56237023, Length=343, Percent_Identity=25.6559766763848, Blast_Score=101, Evalue=9e-22,
Organism=Homo sapiens, GI56118217, Length=343, Percent_Identity=25.6559766763848, Blast_Score=101, Evalue=9e-22,
Organism=Homo sapiens, GI189083684, Length=343, Percent_Identity=25.6559766763848, Blast_Score=101, Evalue=9e-22,
Organism=Escherichia coli, GI48994969, Length=341, Percent_Identity=26.6862170087977, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1788353, Length=347, Percent_Identity=24.4956772334294, Blast_Score=110, Evalue=9e-26,
Organism=Escherichia coli, GI1786974, Length=344, Percent_Identity=25.8720930232558, Blast_Score=110, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI71982038, Length=343, Percent_Identity=27.1137026239067, Blast_Score=119, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI71982035, Length=343, Percent_Identity=27.6967930029155, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17539532, Length=329, Percent_Identity=26.1398176291793, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17568069, Length=333, Percent_Identity=25.2252252252252, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI115532424, Length=324, Percent_Identity=22.5308641975309, Blast_Score=81, Evalue=8e-16,
Organism=Saccharomyces cerevisiae, GI6319493, Length=342, Percent_Identity=26.0233918128655, Blast_Score=88, Evalue=2e-18,
Organism=Drosophila melanogaster, GI19923002, Length=338, Percent_Identity=28.1065088757396, Blast_Score=117, Evalue=9e-27,
Organism=Drosophila melanogaster, GI21356223, Length=328, Percent_Identity=25, Blast_Score=104, Evalue=8e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 36506; Mature: 36506

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVLVTGAAGFIGYHLSQRLLLDRAQVFGIDNLNNYYAVDLKKSRLAQLEPNQNFQFQCL
CEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECHHHHHCCCCCCCCEEEEE
DLSDRNGMETLFESNTFDGVIHLAAQAGVRYSLDNPHAYVDSNLVGFLHILEGCRQSNIS
ECCCCCCHHHHHCCCCCCHHHHEEHHCCCEEECCCCCEEECCCHHHHHHHHHHHHHCCCC
HLVYASSSSVYGANKKVPFSVEDNVDHPVSLYAATKKSNELMAHSYSHLYQIPITGLRFF
EEEEECCCCCCCCCCCCCEECCCCCCCCEEEEEECCCCCHHHHHHHHHEEECCCCCEEEE
TVYGPWGRPDMAYFKFVDAIANNKSIDVYNHGKMQRDFTYIDDVVEGIVRVLHQPPNPDT
EEECCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
TTPPYKLYNIGNNQPVTLMRFIEVIETAMGKTADKNFLPMQPGDVPATYADVDALMNDVG
CCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCC
FQPKTPIEDGIQKFVTWYRSYYQ
CCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRVLVTGAAGFIGYHLSQRLLLDRAQVFGIDNLNNYYAVDLKKSRLAQLEPNQNFQFQCL
CEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECHHHHHCCCCCCCCEEEEE
DLSDRNGMETLFESNTFDGVIHLAAQAGVRYSLDNPHAYVDSNLVGFLHILEGCRQSNIS
ECCCCCCHHHHHCCCCCCHHHHEEHHCCCEEECCCCCEEECCCHHHHHHHHHHHHHCCCC
HLVYASSSSVYGANKKVPFSVEDNVDHPVSLYAATKKSNELMAHSYSHLYQIPITGLRFF
EEEEECCCCCCCCCCCCCEECCCCCCCCEEEEEECCCCCHHHHHHHHHEEECCCCCEEEE
TVYGPWGRPDMAYFKFVDAIANNKSIDVYNHGKMQRDFTYIDDVVEGIVRVLHQPPNPDT
EEECCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
TTPPYKLYNIGNNQPVTLMRFIEVIETAMGKTADKNFLPMQPGDVPATYADVDALMNDVG
CCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCC
FQPKTPIEDGIQKFVTWYRSYYQ
CCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.019 {NAD+}} 0.034 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: TDP; TTP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7682279 [H]