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The map label for this gene is priA [H]

Identifier: 158333512

GI number: 158333512

Start: 282186

End: 284675

Strand: Reverse

Name: priA [H]

Synonym: AM1_0284

Alternate gene names: 158333512

Gene position: 284675-282186 (Counterclockwise)

Preceding gene: 158333513

Following gene: 158333509

Centisome position: 4.38

GC content: 51.81

Gene sequence:

>2490_bases
ATGTCGACACCCAAAGCCTCTCCTTCTCTAGACCGCACTACAGCTGCCCCTTTTGTGGAAGTCCTGGTTGACTGTCCTGG
CACTGACAAGCTCTTTACCTATCGCGTCCCCTCCGATCTGAGTATTCAACCTGGAGATATTCTCAGCGTGCCCTTTGGTG
CTCAGCAAGTTGGAGCCATTGCCATTCGGTTTCTGGCCGATTTGCCCCCGGAGTTAGCTACCACTCGCATTCTGAAGGTG
GTGGATGTGGCTTGCCGCACGTTATTTCCCCCTGGCTATTGGCAACTCTTAGAAAAAGTAGCGGCCTATTATCACACCTC
TTTGATGGCGGTGATTCGAGTGGCATTGCCGCCGGGTTTGTTGCAGCGATCGCAACGCCGTATCCGCTTAAACACGACCC
TTCATCCCCATGACATCAACGGTTTAGGAGCTGCGGCCCAAGGTATTCTAGCGTTATTGCAGCAAAGTCCCAGTCAAGAC
TACACCTGGCAATATCTGCGTCGCCAGATCAAAAAAGCCCAGGTGGGCTTAAGGGAACTTCAGCAGCAAGGCCTTGTCGA
AAGCTATCTGCAAACCCAAGCCCCCACCAAACCGAGGCAACAGCAGGCCGTTACTCTGACTGACCCTAGTGCGCCGGACC
TCACCGTGCGTCAACAGGAAATTTTGACCCTTCTCAAACATGAGGGCGGGGAATTATGGGCCCAAGATCTCTTGAGGCTG
GCCCACACAAGCAGCACCACCCTCAAGAAATTAGCCAGTAAAGGCTGTATCACCCTGCACCAACAGGAGCGACTCCGCTT
AGAAACTTCCCCCATACAGCCCGATCAACCTAGAACCCTGACCCCAGACCAGGCTGGGGTTCTCGCCCAGATTCACCGGC
TCCAGGATTACGCTGAAGTCTTACTCCATGGCGTTACGGGGTCTGGAAAAACAGAAGTCTATTTACAGGCCATCACCCCT
GTCTTAGCGCGAAAACAGTCGGTGATTGTCCTAGTACCAGAAATTGGGCTTACCCCCCAACTAACAGATCGATTTCGGTC
TCGATTTGGCGATCGCATTCGGGTCTATCACAGCGCCCTCTCCGATGGGGAACGCTATGACACCTGGCGGCAGTTTCTGA
CTAGTACTCCCCAAGTAGTGATAGGGACCCGGTCAGCTATTTTCGCTCCTATCGCCAATATTGGCTTAGTCATCTTAGAT
GAAGAGCATGATACGGGCTACAAACAAGATCAACCCGCCCCTTGCTACCATGCCCGCACCGTCGCTCGGTGGCGTGCCCA
ACAAGCAGATTGTCCCCTAATTCTAGGTTCTGCAACCCCTTGCTTAGAGAGTTGGTCCCAAACTCCCCCCGCCAATCCCA
AGCCATCTCAGGCCAGCATTCATTACCTCCGGCTTCCTCGGCGAGTACATGCTCGTCCCATGCCTCCCATCGAAATCGTG
GATATGCGTCTGGAGCTAGCGGATCGGAACCATTCCATCTTTAGTCGACTCCTACAAAAACGCTTACATAGTCTGCAGCA
AAATCGACAGCAGGGGCTATTATTTATGCCTCGTCGGGGGCATAGTACTTTTGTCTCCTGTCGGAGCTGTGGTTATGTCT
CCGAATGTCCCCACTGCAGCGTTTCTCTGACCTATCACCATAGCCCCGATTCCCGTCAAACCCTGTTGCGGTGCCATTAC
TGCAATTATGCTCAGCCTCAACCAGAAGTATGTCCTGTCTGCAAATCCTCTTATTTCCGCTTTTTTGGTAGCGGTACGCA
ACGGGTCATGGAGGAACTTGCCAAAACCTTTCCTGAACTACGGGTGATTCGCTTTGATAGTGACACCACCCGCCGCAAAG
GTGCTCATCGTCAGCTCCTCAATCAGTTTGCCCGCGGGGAGGCTGATCTAATGGTTGGCACCCAAATGCTGACGAAAGGG
ATTGATCTCCCTCAAGTTCAGCTCATTGGTGTTTTGGCTGCGGATAGTTTGCTCAACATCCCCGATTTTCGAGCCAGTGA
ACGCACCTTTCAGACCCTGTTGCAAGTTGCAGGACGGGCTGGCCGAGGGGAGCATCCGGGGCAAGTGCTGCTCCAAACCT
ATGCTCCAGAACATCCTGTTATCCAAGCTGTTCAAAAATATGAAATGGATAGCTTTCTGTTCGCTGAACTCAAACAGCGT
CAGGAAGCTAATTATCCGCCGTTTCATCAGCTCATTCTCTTACGGATTAGTGGTCTAAATGAAATGATGGTGGAGAAGAC
TGCTCTAGAGATTGCCAATTATTTGCACGCTTATATCCATACTCACTGCTATCAGGGCACCATACTAGGTCCTGCACCTG
CCAATATTTTGAGGGTAGCTCGTCGTTATTATTGGCATGTATTAATCAAACGGCCCCAAACGGAAATGGGGCAAACCGAA
CTTTGGCCCTTCCCTATGCAGGGATGCCAACGACGATGTCCACAGAATATTCGTCTCCAAGTGGATGTTGATCCACTCAA
ACTCCTATAA

Upstream 100 bases:

>100_bases
CTGCCATGGTGGGCAAATTTGGTTTGATGCAGTGGTAAATCAAGGTGCTACATTTTATTGGACCCTGGCTTACAAACAAT
AAACTCCAGGGTGCGCCTAT

Downstream 100 bases:

>100_bases
GAATGCAGCCTCAACCGAAAAGCGCACCCTTGAAGATGCGCTTTTCGGTTGAGATAAGCCTCTAATACTGATGTGAGACA
GTGTTTAGAGATTGAAAGCG

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 829; Mature: 828

Protein sequence:

>829_residues
MSTPKASPSLDRTTAAPFVEVLVDCPGTDKLFTYRVPSDLSIQPGDILSVPFGAQQVGAIAIRFLADLPPELATTRILKV
VDVACRTLFPPGYWQLLEKVAAYYHTSLMAVIRVALPPGLLQRSQRRIRLNTTLHPHDINGLGAAAQGILALLQQSPSQD
YTWQYLRRQIKKAQVGLRELQQQGLVESYLQTQAPTKPRQQQAVTLTDPSAPDLTVRQQEILTLLKHEGGELWAQDLLRL
AHTSSTTLKKLASKGCITLHQQERLRLETSPIQPDQPRTLTPDQAGVLAQIHRLQDYAEVLLHGVTGSGKTEVYLQAITP
VLARKQSVIVLVPEIGLTPQLTDRFRSRFGDRIRVYHSALSDGERYDTWRQFLTSTPQVVIGTRSAIFAPIANIGLVILD
EEHDTGYKQDQPAPCYHARTVARWRAQQADCPLILGSATPCLESWSQTPPANPKPSQASIHYLRLPRRVHARPMPPIEIV
DMRLELADRNHSIFSRLLQKRLHSLQQNRQQGLLFMPRRGHSTFVSCRSCGYVSECPHCSVSLTYHHSPDSRQTLLRCHY
CNYAQPQPEVCPVCKSSYFRFFGSGTQRVMEELAKTFPELRVIRFDSDTTRRKGAHRQLLNQFARGEADLMVGTQMLTKG
IDLPQVQLIGVLAADSLLNIPDFRASERTFQTLLQVAGRAGRGEHPGQVLLQTYAPEHPVIQAVQKYEMDSFLFAELKQR
QEANYPPFHQLILLRISGLNEMMVEKTALEIANYLHAYIHTHCYQGTILGPAPANILRVARRYYWHVLIKRPQTEMGQTE
LWPFPMQGCQRRCPQNIRLQVDVDPLKLL

Sequences:

>Translated_829_residues
MSTPKASPSLDRTTAAPFVEVLVDCPGTDKLFTYRVPSDLSIQPGDILSVPFGAQQVGAIAIRFLADLPPELATTRILKV
VDVACRTLFPPGYWQLLEKVAAYYHTSLMAVIRVALPPGLLQRSQRRIRLNTTLHPHDINGLGAAAQGILALLQQSPSQD
YTWQYLRRQIKKAQVGLRELQQQGLVESYLQTQAPTKPRQQQAVTLTDPSAPDLTVRQQEILTLLKHEGGELWAQDLLRL
AHTSSTTLKKLASKGCITLHQQERLRLETSPIQPDQPRTLTPDQAGVLAQIHRLQDYAEVLLHGVTGSGKTEVYLQAITP
VLARKQSVIVLVPEIGLTPQLTDRFRSRFGDRIRVYHSALSDGERYDTWRQFLTSTPQVVIGTRSAIFAPIANIGLVILD
EEHDTGYKQDQPAPCYHARTVARWRAQQADCPLILGSATPCLESWSQTPPANPKPSQASIHYLRLPRRVHARPMPPIEIV
DMRLELADRNHSIFSRLLQKRLHSLQQNRQQGLLFMPRRGHSTFVSCRSCGYVSECPHCSVSLTYHHSPDSRQTLLRCHY
CNYAQPQPEVCPVCKSSYFRFFGSGTQRVMEELAKTFPELRVIRFDSDTTRRKGAHRQLLNQFARGEADLMVGTQMLTKG
IDLPQVQLIGVLAADSLLNIPDFRASERTFQTLLQVAGRAGRGEHPGQVLLQTYAPEHPVIQAVQKYEMDSFLFAELKQR
QEANYPPFHQLILLRISGLNEMMVEKTALEIANYLHAYIHTHCYQGTILGPAPANILRVARRYYWHVLIKRPQTEMGQTE
LWPFPMQGCQRRCPQNIRLQVDVDPLKLL
>Mature_828_residues
STPKASPSLDRTTAAPFVEVLVDCPGTDKLFTYRVPSDLSIQPGDILSVPFGAQQVGAIAIRFLADLPPELATTRILKVV
DVACRTLFPPGYWQLLEKVAAYYHTSLMAVIRVALPPGLLQRSQRRIRLNTTLHPHDINGLGAAAQGILALLQQSPSQDY
TWQYLRRQIKKAQVGLRELQQQGLVESYLQTQAPTKPRQQQAVTLTDPSAPDLTVRQQEILTLLKHEGGELWAQDLLRLA
HTSSTTLKKLASKGCITLHQQERLRLETSPIQPDQPRTLTPDQAGVLAQIHRLQDYAEVLLHGVTGSGKTEVYLQAITPV
LARKQSVIVLVPEIGLTPQLTDRFRSRFGDRIRVYHSALSDGERYDTWRQFLTSTPQVVIGTRSAIFAPIANIGLVILDE
EHDTGYKQDQPAPCYHARTVARWRAQQADCPLILGSATPCLESWSQTPPANPKPSQASIHYLRLPRRVHARPMPPIEIVD
MRLELADRNHSIFSRLLQKRLHSLQQNRQQGLLFMPRRGHSTFVSCRSCGYVSECPHCSVSLTYHHSPDSRQTLLRCHYC
NYAQPQPEVCPVCKSSYFRFFGSGTQRVMEELAKTFPELRVIRFDSDTTRRKGAHRQLLNQFARGEADLMVGTQMLTKGI
DLPQVQLIGVLAADSLLNIPDFRASERTFQTLLQVAGRAGRGEHPGQVLLQTYAPEHPVIQAVQKYEMDSFLFAELKQRQ
EANYPPFHQLILLRISGLNEMMVEKTALEIANYLHAYIHTHCYQGTILGPAPANILRVARRYYWHVLIKRPQTEMGQTEL
WPFPMQGCQRRCPQNIRLQVDVDPLKLL

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:
GO:0000166: Primosomal protein N'
GO:0003676: Primosomal protein N'
GO:0003677: Primosomal protein N'
GO:0004386: Primosomal protein N'
GO:0005524: Primosomal protein N'
GO:0006260: Primosomal protein N'
GO:0008026: Primosomal protein N'
GO:0016787: Primosomal protein N'

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=580, Percent_Identity=36.7241379310345, Blast_Score=346, Evalue=4e-96,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 93618; Mature: 93487

Theoretical pI: Translated: 9.37; Mature: 9.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTPKASPSLDRTTAAPFVEVLVDCPGTDKLFTYRVPSDLSIQPGDILSVPFGAQQVGAI
CCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCEEECCCCHHHHHHH
AIRFLADLPPELATTRILKVVDVACRTLFPPGYWQLLEKVAAYYHTSLMAVIRVALPPGL
HHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
LQRSQRRIRLNTTLHPHDINGLGAAAQGILALLQQSPSQDYTWQYLRRQIKKAQVGLREL
HHHCCCEEEEECEECCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
QQQGLVESYLQTQAPTKPRQQQAVTLTDPSAPDLTVRQQEILTLLKHEGGELWAQDLLRL
HHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHH
AHTSSTTLKKLASKGCITLHQQERLRLETSPIQPDQPRTLTPDQAGVLAQIHRLQDYAEV
HHCCHHHHHHHHCCCCEEEEHHHHHEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
LLHGVTGSGKTEVYLQAITPVLARKQSVIVLVPEIGLTPQLTDRFRSRFGDRIRVYHSAL
HHHCCCCCCCCEEHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHCCHHHHHHHHH
SDGERYDTWRQFLTSTPQVVIGTRSAIFAPIANIGLVILDEEHDTGYKQDQPAPCYHART
CCCHHHHHHHHHHCCCCCEEEECCHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCHHHHH
VARWRAQQADCPLILGSATPCLESWSQTPPANPKPSQASIHYLRLPRRVHARPMPPIEIV
HHHHHHHCCCCCEEECCCCHHHHHHCCCCCCCCCCCCCCCHHEECCHHHCCCCCCCHHHH
DMRLELADRNHSIFSRLLQKRLHSLQQNRQQGLLFMPRRGHSTFVSCRSCGYVSECPHCS
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHCCCCCCCCCCCE
VSLTYHHSPDSRQTLLRCHYCNYAQPQPEVCPVCKSSYFRFFGSGTQRVMEELAKTFPEL
EEEEEECCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCE
RVIRFDSDTTRRKGAHRQLLNQFARGEADLMVGTQMLTKGIDLPQVQLIGVLAADSLLNI
EEEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCC
PDFRASERTFQTLLQVAGRAGRGEHPGQVLLQTYAPEHPVIQAVQKYEMDSFLFAELKQR
CCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
QEANYPPFHQLILLRISGLNEMMVEKTALEIANYLHAYIHTHCYQGTILGPAPANILRVA
HCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCHHHHHHH
RRYYWHVLIKRPQTEMGQTELWPFPMQGCQRRCPQNIRLQVDVDPLKLL
HHHHHHHEEECCCCCCCCCCCCCCCHHHHHHHCCCCCEEEEECCHHHCC
>Mature Secondary Structure 
STPKASPSLDRTTAAPFVEVLVDCPGTDKLFTYRVPSDLSIQPGDILSVPFGAQQVGAI
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCEEECCCCHHHHHHH
AIRFLADLPPELATTRILKVVDVACRTLFPPGYWQLLEKVAAYYHTSLMAVIRVALPPGL
HHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
LQRSQRRIRLNTTLHPHDINGLGAAAQGILALLQQSPSQDYTWQYLRRQIKKAQVGLREL
HHHCCCEEEEECEECCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
QQQGLVESYLQTQAPTKPRQQQAVTLTDPSAPDLTVRQQEILTLLKHEGGELWAQDLLRL
HHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHH
AHTSSTTLKKLASKGCITLHQQERLRLETSPIQPDQPRTLTPDQAGVLAQIHRLQDYAEV
HHCCHHHHHHHHCCCCEEEEHHHHHEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
LLHGVTGSGKTEVYLQAITPVLARKQSVIVLVPEIGLTPQLTDRFRSRFGDRIRVYHSAL
HHHCCCCCCCCEEHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHCCHHHHHHHHH
SDGERYDTWRQFLTSTPQVVIGTRSAIFAPIANIGLVILDEEHDTGYKQDQPAPCYHART
CCCHHHHHHHHHHCCCCCEEEECCHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCHHHHH
VARWRAQQADCPLILGSATPCLESWSQTPPANPKPSQASIHYLRLPRRVHARPMPPIEIV
HHHHHHHCCCCCEEECCCCHHHHHHCCCCCCCCCCCCCCCHHEECCHHHCCCCCCCHHHH
DMRLELADRNHSIFSRLLQKRLHSLQQNRQQGLLFMPRRGHSTFVSCRSCGYVSECPHCS
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHCCCCCCCCCCCE
VSLTYHHSPDSRQTLLRCHYCNYAQPQPEVCPVCKSSYFRFFGSGTQRVMEELAKTFPEL
EEEEEECCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCE
RVIRFDSDTTRRKGAHRQLLNQFARGEADLMVGTQMLTKGIDLPQVQLIGVLAADSLLNI
EEEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCC
PDFRASERTFQTLLQVAGRAGRGEHPGQVLLQTYAPEHPVIQAVQKYEMDSFLFAELKQR
CCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
QEANYPPFHQLILLRISGLNEMMVEKTALEIANYLHAYIHTHCYQGTILGPAPANILRVA
HCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCHHHHHHH
RRYYWHVLIKRPQTEMGQTELWPFPMQGCQRRCPQNIRLQVDVDPLKLL
HHHHHHHEEECCCCCCCCCCCCCCCHHHHHHHCCCCCEEEEECCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]