Click here to switch to the map view.

The map label for this gene is ycgF [H]

Identifier: 158333446

GI number: 158333446

Start: 213921

End: 214634

Strand: Reverse

Name: ycgF [H]

Synonym: AM1_0218

Alternate gene names: 158333446

Gene position: 214634-213921 (Counterclockwise)

Preceding gene: 158333449

Following gene: 158333443

Centisome position: 3.3

GC content: 42.16

Gene sequence:

>714_bases
ATGGCCTTTCAGCCCATTGTGAATCTTGCAGATGGCAGTATTTTTGCGCAAGAAGCGCTGGCTCGAGGCATGAATAATGA
ATCAGCGATCAGTATTTTGGAGCAAGTAACGGATGAGAACCTATATCGCTTTGATCAAGCTTGTCGGGTTCAGGCGATTC
AGCTTGCCGCAGAGTTAGACATTCAATCGATATTGAGTATCAACTTTTTGCCCAATGCAGTGTACCATCCAGAACTTTGT
CTTCAGACAACACTTGAAACGGCTGAACGATGTAATTTTCCCATTAGCCGAATTCTATTTGAGCTAACGGAAGTTGAAAA
GGTTATAGATTATGCCCATGTTCAATCCATCATTAAGCACTATCAAAAGTTGGGGTTTAGAACAGCGTTGGATGACTTTG
GAGCCGGTTATTCTGGGTTTAACTTTCTGGCTGATTTCCGACCTGACTTTATTAAGATAGACATGGCTTTGATTCGGAAT
ATTGATAAAGACAAAGCGCGGCAGGCCATTGTTCGAGGAATTGTGCAAGTCTGTCGAGATTTAGCCATTGAACCGATTGC
AGAAGGCATTGAAACAGATTTAGAATTAGACGTTCTTAGGGAATTAGGTCTCCAATTATTTCAGGGCTATTACTTTGCAA
AGCCTAGCTTCAGAGCTGTGACTACCGACTTAAGCTTTGCCCGCAGCAATCCTAACCTCCATAAAAATGATTGA

Upstream 100 bases:

>100_bases
AGCCTCATGTCCAAGAATTCCAGCTGCGGTTGTGTAGAGTTCAATGGCTAGGGAGTGTGACGGTTGTCTGAATTCGTCTA
GGCTGAACTTTCGCTTCACA

Downstream 100 bases:

>100_bases
TTCCAATGAAATAATACAATCTAATGCTGGTTCTTGAAGAAAGCGCATCAAAGCCTAGTTACAGGAAAGCAGCCAATACC
AATTTCGTATAGAGATGAGC

Product: EAL domain-containing protein

Products: NA

Alternate protein names: Blrp [H]

Number of amino acids: Translated: 237; Mature: 236

Protein sequence:

>237_residues
MAFQPIVNLADGSIFAQEALARGMNNESAISILEQVTDENLYRFDQACRVQAIQLAAELDIQSILSINFLPNAVYHPELC
LQTTLETAERCNFPISRILFELTEVEKVIDYAHVQSIIKHYQKLGFRTALDDFGAGYSGFNFLADFRPDFIKIDMALIRN
IDKDKARQAIVRGIVQVCRDLAIEPIAEGIETDLELDVLRELGLQLFQGYYFAKPSFRAVTTDLSFARSNPNLHKND

Sequences:

>Translated_237_residues
MAFQPIVNLADGSIFAQEALARGMNNESAISILEQVTDENLYRFDQACRVQAIQLAAELDIQSILSINFLPNAVYHPELC
LQTTLETAERCNFPISRILFELTEVEKVIDYAHVQSIIKHYQKLGFRTALDDFGAGYSGFNFLADFRPDFIKIDMALIRN
IDKDKARQAIVRGIVQVCRDLAIEPIAEGIETDLELDVLRELGLQLFQGYYFAKPSFRAVTTDLSFARSNPNLHKND
>Mature_236_residues
AFQPIVNLADGSIFAQEALARGMNNESAISILEQVTDENLYRFDQACRVQAIQLAAELDIQSILSINFLPNAVYHPELCL
QTTLETAERCNFPISRILFELTEVEKVIDYAHVQSIIKHYQKLGFRTALDDFGAGYSGFNFLADFRPDFIKIDMALIRNI
DKDKARQAIVRGIVQVCRDLAIEPIAEGIETDLELDVLRELGLQLFQGYYFAKPSFRAVTTDLSFARSNPNLHKND

Specific function: Binds to and releases the YcgE repressor from its bound DNA target in a blue-light-dependent (470 nm) fashion. A shift to low temperature also triggers a YcgF-medisted relief of repression by YcgE, suggesting YcgF may serve as a thermometer. Blue light ma

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 EAL domain [H]

Homologues:

Organism=Escherichia coli, GI1787410, Length=222, Percent_Identity=30.6306306306306, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1790496, Length=227, Percent_Identity=28.1938325991189, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI87081845, Length=228, Percent_Identity=31.140350877193, Blast_Score=88, Evalue=4e-19,
Organism=Escherichia coli, GI226510982, Length=226, Percent_Identity=26.1061946902655, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787541, Length=124, Percent_Identity=40.3225806451613, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI87081980, Length=144, Percent_Identity=31.25, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI87081743, Length=220, Percent_Identity=29.5454545454545, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1788849, Length=150, Percent_Identity=28.6666666666667, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1786507, Length=119, Percent_Identity=31.9327731092437, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI87082096, Length=150, Percent_Identity=28, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI87081921, Length=225, Percent_Identity=24.8888888888889, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787055, Length=231, Percent_Identity=29.8701298701299, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1788502, Length=119, Percent_Identity=28.5714285714286, Blast_Score=67, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001792
- InterPro:   IPR007024
- InterPro:   IPR001633 [H]

Pfam domain/function: PF04940 BLUF; PF00563 EAL [H]

EC number: NA

Molecular weight: Translated: 26810; Mature: 26679

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS50883 EAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFQPIVNLADGSIFAQEALARGMNNESAISILEQVTDENLYRFDQACRVQAIQLAAELD
CCCCCHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IQSILSINFLPNAVYHPELCLQTTLETAERCNFPISRILFELTEVEKVIDYAHVQSIIKH
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
YQKLGFRTALDDFGAGYSGFNFLADFRPDFIKIDMALIRNIDKDKARQAIVRGIVQVCRD
HHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
LAIEPIAEGIETDLELDVLRELGLQLFQGYYFAKPSFRAVTTDLSFARSNPNLHKND
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
AFQPIVNLADGSIFAQEALARGMNNESAISILEQVTDENLYRFDQACRVQAIQLAAELD
CCCCHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IQSILSINFLPNAVYHPELCLQTTLETAERCNFPISRILFELTEVEKVIDYAHVQSIIKH
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
YQKLGFRTALDDFGAGYSGFNFLADFRPDFIKIDMALIRNIDKDKARQAIVRGIVQVCRD
HHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
LAIEPIAEGIETDLELDVLRELGLQLFQGYYFAKPSFRAVTTDLSFARSNPNLHKND
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]