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The map label for this gene is hisF [H]

Identifier: 229595062

GI number: 229595062

Start: 2304741

End: 2305505

Strand: Direct

Name: hisF [H]

Synonym: Franean1_1924

Alternate gene names: 229595062

Gene position: 2304741-2305505 (Clockwise)

Preceding gene: 158313758

Following gene: 158313760

Centisome position: 25.66

GC content: 73.73

Gene sequence:

>765_bases
GTGACCGTCGCCGTCCGGGTGATCCCCTGCCTTGACGTCGACGGCGGGCGGGTCGTCAAGGGTGTGAACTTCACCGACCT
GCGGGACGCGGGCGACCCGGTCGAGATGGCCCGGCTCTACGACGCCGAGGGCGCCGACGAGCTGACCTTCCTCGACATCA
CCGCGTCCAGCGGAAACCGGGAGACCACGTACGACATCGTCCGGCGCACGGCGGAGCAGGTCTTCATCCCGCTCACCGTC
GGCGGCGGGGTGCGCTCGGTCGACGACGTCGACCGGCTGCTGCGCGCCGGCGCCGACAAGGTGGGGATCAACACCGCAGC
CGTCGCCCGCCCGGAGCTGGTCCGCGAGTGCGCGCTGCGGTTCGGCAGCCAGTGCATCGTGCTCTCGGTGGACGCCCGCC
GCTCCCGCGAGCCGGGCGGCCCCGCCTTCGAGGTGACCACCCACGGCGGGCGCCAGGGCACCGGCATCGACGCGGTCGCC
TGGGCCGCGCGGGCCGTCGAGCTGGGCGCCGGCGAGATCCTGCTGAACTCGATGGACGCCGACGGCACCCAGGACGGCTA
CGACCTGGAGATGATCACGTCGGTCCGCGCGGCGGTGGACGTCCCGGTGATCGCCAGCGGTGGCGCCGGCAGCCTCGACC
ACTTCGCCCCCGCGGTCGGCGCCGGCGCCGACGCCGTGCTGGCCGCCAGCGTCTTCCACTTCGGGCGGATGCGGATCTCC
GAGGTGAAGAAGGTGCTGCGCGACGCCGCCATCGCGGTGCGCTGA

Upstream 100 bases:

>100_bases
GTGTCGAGGGCGCCATTGTCGGCAAGGCGCTCTACGCCGGCGCGTTCACCCTGCCCGAGGCGCTCGCGGTCGCGGCGGAG
GTGCCCGCGGCGGGCGCACC

Downstream 100 bases:

>100_bases
CGGCTGCGGGCAACGCGCGGCCGGCTTGCGGCGCGGACGGCTGCGGCCGGGCTGACGGCTGCGGCCGGGGCACGGATCGG
CTGGTGGCGCCGGCCGTGGG

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 254; Mature: 253

Protein sequence:

>254_residues
MTVAVRVIPCLDVDGGRVVKGVNFTDLRDAGDPVEMARLYDAEGADELTFLDITASSGNRETTYDIVRRTAEQVFIPLTV
GGGVRSVDDVDRLLRAGADKVGINTAAVARPELVRECALRFGSQCIVLSVDARRSREPGGPAFEVTTHGGRQGTGIDAVA
WAARAVELGAGEILLNSMDADGTQDGYDLEMITSVRAAVDVPVIASGGAGSLDHFAPAVGAGADAVLAASVFHFGRMRIS
EVKKVLRDAAIAVR

Sequences:

>Translated_254_residues
MTVAVRVIPCLDVDGGRVVKGVNFTDLRDAGDPVEMARLYDAEGADELTFLDITASSGNRETTYDIVRRTAEQVFIPLTV
GGGVRSVDDVDRLLRAGADKVGINTAAVARPELVRECALRFGSQCIVLSVDARRSREPGGPAFEVTTHGGRQGTGIDAVA
WAARAVELGAGEILLNSMDADGTQDGYDLEMITSVRAAVDVPVIASGGAGSLDHFAPAVGAGADAVLAASVFHFGRMRIS
EVKKVLRDAAIAVR
>Mature_253_residues
TVAVRVIPCLDVDGGRVVKGVNFTDLRDAGDPVEMARLYDAEGADELTFLDITASSGNRETTYDIVRRTAEQVFIPLTVG
GGVRSVDDVDRLLRAGADKVGINTAAVARPELVRECALRFGSQCIVLSVDARRSREPGGPAFEVTTHGGRQGTGIDAVAW
AARAVELGAGEILLNSMDADGTQDGYDLEMITSVRAAVDVPVIASGGAGSLDHFAPAVGAGADAVLAASVFHFGRMRISE
VKKVLRDAAIAVR

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=257, Percent_Identity=44.3579766536965, Blast_Score=204, Evalue=5e-54,
Organism=Escherichia coli, GI87082028, Length=247, Percent_Identity=27.9352226720648, Blast_Score=89, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6319725, Length=312, Percent_Identity=37.1794871794872, Blast_Score=160, Evalue=3e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.-

Molecular weight: Translated: 26608; Mature: 26477

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVAVRVIPCLDVDGGRVVKGVNFTDLRDAGDPVEMARLYDAEGADELTFLDITASSGNR
CEEEEEEEEEEECCCCEEEECCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEEEECCCCC
ETTYDIVRRTAEQVFIPLTVGGGVRSVDDVDRLLRAGADKVGINTAAVARPELVRECALR
CHHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHCCCCCCCCCHHHHCCHHHHHHHHHH
FGSQCIVLSVDARRSREPGGPAFEVTTHGGRQGTGIDAVAWAARAVELGAGEILLNSMDA
CCCEEEEEEECCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCC
DGTQDGYDLEMITSVRAAVDVPVIASGGAGSLDHFAPAVGAGADAVLAASVFHFGRMRIS
CCCCCCCCHHHHHHHHHHHCCCEEECCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
EVKKVLRDAAIAVR
HHHHHHHHHHHCCC
>Mature Secondary Structure 
TVAVRVIPCLDVDGGRVVKGVNFTDLRDAGDPVEMARLYDAEGADELTFLDITASSGNR
EEEEEEEEEEECCCCEEEECCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEEEECCCCC
ETTYDIVRRTAEQVFIPLTVGGGVRSVDDVDRLLRAGADKVGINTAAVARPELVRECALR
CHHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHCCCCCCCCCHHHHCCHHHHHHHHHH
FGSQCIVLSVDARRSREPGGPAFEVTTHGGRQGTGIDAVAWAARAVELGAGEILLNSMDA
CCCEEEEEEECCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCC
DGTQDGYDLEMITSVRAAVDVPVIASGGAGSLDHFAPAVGAGADAVLAASVFHFGRMRIS
CCCCCCCCHHHHHHHHHHHCCCEEECCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
EVKKVLRDAAIAVR
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA