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The map label for this gene is murF [H]

Identifier: 158318858

GI number: 158318858

Start: 8719908

End: 8721251

Strand: Direct

Name: murF [H]

Synonym: Franean1_7139

Alternate gene names: 158318858

Gene position: 8719908-8721251 (Clockwise)

Preceding gene: 158318853

Following gene: 158318859

Centisome position: 97.08

GC content: 75.82

Gene sequence:

>1344_bases
ATGATCCCGCTCAGCCTCGGCGAGATCGCCGCGGTCGTCGGCGGGACGGTCGCGGGCGACGGCGCCGTGACGGTGACCGC
GCCAGCCGTGCTCGACGGCCGGCAGGCCGAGCCGGGCGGCCTGTTCGTCGCCTTCGCCGGCGAGCACGTCGACGGCCACG
ACTACGCCGGCCAGGCCGGCCGGGCCGGCGCGGTGGCCACGCTCGGCTCCCGGCCCACGCCGCTGCCCACCGTCGTCGTC
GAGGACGCCCAGTCCGCGCTGCAGGCGCTCGCCGCCCACGTCGTGGCCCGGCTGCGCGACGGGCTGACCGTTGTCGGGCT
GACCGGCTCCCAGGGTAAGACCAGCACCAAGGACCTGCTGGCGGCCGTGCTGTCGAGCGCCGCGCCGACGATCGCCACGA
TCGGCTCGCTCAACAACGAGCTCGGTGTGCCGCTCACCATGCTGCGCGCCGACCCGGCCACCCGGTTCCTCGCCCTGGAG
ATGGGCGCCCGCCACATCGGCGACATCGCCGAGCTCACCGGCCTGGTCGCACCCGACATCGCCGTCGTCCTCAACGTCGG
CCAGGCCCACCTCGGCGAGTTCGGGTCGCGCGAGGCCATCGCCAGGACCAAGGGCGAGCTGGTGCAGGGGCTGGCGCCCG
GCGGCACCGCCGTCCTGGGCGCCGACGATCCCCGGGTGGTCGCGATGCGCTCGCTCACCGACGGTCCGGTGCTGACCTTC
GGCCGGGTCGAGCACGCCGACGTGCGCGTGCTCGACCTGGCGCTGGACCGGCTCGGCCGGCCGTCCTTCACGCTGCGGAC
CGCCGACGCCTCGGCTCCCGTCACGCTGCCGCTCGTCGGCGGCCACCAGGCACTCAACGCCTCGGCTGCCGCGGCGGCGG
GGCTGGCGGCCGGCGTACCCCTCGACGTGGCAGCGGCGGCGCTGGCCACCGCCTCGCTGTCGCAGTGGCGCATGGAGCTA
CGCGATCTCGCCTGCGGCGCGACGCTGCTCAACGACTCGTACAACGCCAACCCCGACTCGACCCGCGCCGCCCTGGACGC
GCTGGCAGCGATCGAGGGAGGGCGCCGCATCGCCGTTCTCGGCGAGATGCGCGAGCTCGGCGACGGCAGCGAGGCCGAGC
ACCGCGCCGTCGGGGAGTACGCCGCCTCCCGGGCCGACGTGGTGGTCGCGGTCGGCGAGGCCGCCCGACCGATCGCCGAC
GGCGCGGGAGAACGGGTGGTGGCGCTGGCTGACAACAACGCGGCCGTTGACTGGCTACGCGGCCACCTCGCCGCCGGCGA
GGTGGTGCTCGTCAAGGCCTCCCGCGGGGCGCGCCTCGACGAGGTAGCCGTCGCACTCGCGTAG

Upstream 100 bases:

>100_bases
GATCGGCACCATACGGGAGCATGACCCGCAGAGAACCAGCCTCCGTCGACCGCTACCTCGTCAGCCTGCTCCCGCTCGCC
GGCCGCCGCCCCACCTGGGC

Downstream 100 bases:

>100_bases
TTGGCGGGACGCACCAGAAGATCCGCTTCGCCGTGCTGTTCGGGCCCAACGTCCAGGAGTTGCCGAAGCCGAACAGGAAA
CTGAGCCCCATCACCAGCAC

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate--D-alanyl-D-alanyl ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MIPLSLGEIAAVVGGTVAGDGAVTVTAPAVLDGRQAEPGGLFVAFAGEHVDGHDYAGQAGRAGAVATLGSRPTPLPTVVV
EDAQSALQALAAHVVARLRDGLTVVGLTGSQGKTSTKDLLAAVLSSAAPTIATIGSLNNELGVPLTMLRADPATRFLALE
MGARHIGDIAELTGLVAPDIAVVLNVGQAHLGEFGSREAIARTKGELVQGLAPGGTAVLGADDPRVVAMRSLTDGPVLTF
GRVEHADVRVLDLALDRLGRPSFTLRTADASAPVTLPLVGGHQALNASAAAAAGLAAGVPLDVAAAALATASLSQWRMEL
RDLACGATLLNDSYNANPDSTRAALDALAAIEGGRRIAVLGEMRELGDGSEAEHRAVGEYAASRADVVVAVGEAARPIAD
GAGERVVALADNNAAVDWLRGHLAAGEVVLVKASRGARLDEVAVALA

Sequences:

>Translated_447_residues
MIPLSLGEIAAVVGGTVAGDGAVTVTAPAVLDGRQAEPGGLFVAFAGEHVDGHDYAGQAGRAGAVATLGSRPTPLPTVVV
EDAQSALQALAAHVVARLRDGLTVVGLTGSQGKTSTKDLLAAVLSSAAPTIATIGSLNNELGVPLTMLRADPATRFLALE
MGARHIGDIAELTGLVAPDIAVVLNVGQAHLGEFGSREAIARTKGELVQGLAPGGTAVLGADDPRVVAMRSLTDGPVLTF
GRVEHADVRVLDLALDRLGRPSFTLRTADASAPVTLPLVGGHQALNASAAAAAGLAAGVPLDVAAAALATASLSQWRMEL
RDLACGATLLNDSYNANPDSTRAALDALAAIEGGRRIAVLGEMRELGDGSEAEHRAVGEYAASRADVVVAVGEAARPIAD
GAGERVVALADNNAAVDWLRGHLAAGEVVLVKASRGARLDEVAVALA
>Mature_447_residues
MIPLSLGEIAAVVGGTVAGDGAVTVTAPAVLDGRQAEPGGLFVAFAGEHVDGHDYAGQAGRAGAVATLGSRPTPLPTVVV
EDAQSALQALAAHVVARLRDGLTVVGLTGSQGKTSTKDLLAAVLSSAAPTIATIGSLNNELGVPLTMLRADPATRFLALE
MGARHIGDIAELTGLVAPDIAVVLNVGQAHLGEFGSREAIARTKGELVQGLAPGGTAVLGADDPRVVAMRSLTDGPVLTF
GRVEHADVRVLDLALDRLGRPSFTLRTADASAPVTLPLVGGHQALNASAAAAAGLAAGVPLDVAAAALATASLSQWRMEL
RDLACGATLLNDSYNANPDSTRAALDALAAIEGGRRIAVLGEMRELGDGSEAEHRAVGEYAASRADVVVAVGEAARPIAD
GAGERVVALADNNAAVDWLRGHLAAGEVVLVKASRGARLDEVAVALA

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=456, Percent_Identity=40.1315789473684, Blast_Score=228, Evalue=8e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 44906; Mature: 44906

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPLSLGEIAAVVGGTVAGDGAVTVTAPAVLDGRQAEPGGLFVAFAGEHVDGHDYAGQAG
CCCCCHHHHHHHHCCCEECCCEEEEEECCEECCCCCCCCCEEEEEECCCCCCCCCCCCCC
RAGAVATLGSRPTPLPTVVVEDAQSALQALAAHVVARLRDGLTVVGLTGSQGKTSTKDLL
CCCCEEECCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHH
AAVLSSAAPTIATIGSLNNELGVPLTMLRADPATRFLALEMGARHIGDIAELTGLVAPDI
HHHHHCCCCHHHHHHCCCCCCCCCEEEEECCCCCEEEEEHHCCHHHCCHHHHHHHCCCCC
AVVLNVGQAHLGEFGSREAIARTKGELVQGLAPGGTAVLGADDPRVVAMRSLTDGPVLTF
EEEEECCHHHHCCCCCHHHHHHHHHHHHHCCCCCCCEEECCCCCCEEEEECCCCCCEEEE
GRVEHADVRVLDLALDRLGRPSFTLRTADASAPVTLPLVGGHQALNASAAAAAGLAAGVP
CCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCEEEEEECCCHHHCCHHHHHHHHHCCCC
LDVAAAALATASLSQWRMELRDLACGATLLNDSYNANPDSTRAALDALAAIEGGRRIAVL
HHHHHHHHHHHHHHHHHHHHHHHHHCHHEECCCCCCCCCHHHHHHHHHHHHCCCCEEEEE
GEMRELGDGSEAEHRAVGEYAASRADVVVAVGEAARPIADGAGERVVALADNNAAVDWLR
EHHHHCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCEEEEEECCCCHHHHHH
GHLAAGEVVLVKASRGARLDEVAVALA
HHCCCCCEEEEECCCCCCHHHHHEECC
>Mature Secondary Structure
MIPLSLGEIAAVVGGTVAGDGAVTVTAPAVLDGRQAEPGGLFVAFAGEHVDGHDYAGQAG
CCCCCHHHHHHHHCCCEECCCEEEEEECCEECCCCCCCCCEEEEEECCCCCCCCCCCCCC
RAGAVATLGSRPTPLPTVVVEDAQSALQALAAHVVARLRDGLTVVGLTGSQGKTSTKDLL
CCCCEEECCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHH
AAVLSSAAPTIATIGSLNNELGVPLTMLRADPATRFLALEMGARHIGDIAELTGLVAPDI
HHHHHCCCCHHHHHHCCCCCCCCCEEEEECCCCCEEEEEHHCCHHHCCHHHHHHHCCCCC
AVVLNVGQAHLGEFGSREAIARTKGELVQGLAPGGTAVLGADDPRVVAMRSLTDGPVLTF
EEEEECCHHHHCCCCCHHHHHHHHHHHHHCCCCCCCEEECCCCCCEEEEECCCCCCEEEE
GRVEHADVRVLDLALDRLGRPSFTLRTADASAPVTLPLVGGHQALNASAAAAAGLAAGVP
CCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCEEEEEECCCHHHCCHHHHHHHHHCCCC
LDVAAAALATASLSQWRMELRDLACGATLLNDSYNANPDSTRAALDALAAIEGGRRIAVL
HHHHHHHHHHHHHHHHHHHHHHHHHCHHEECCCCCCCCCHHHHHHHHHHHHCCCCEEEEE
GEMRELGDGSEAEHRAVGEYAASRADVVVAVGEAARPIADGAGERVVALADNNAAVDWLR
EHHHHCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCEEEEEECCCCHHHHHH
GHLAAGEVVLVKASRGARLDEVAVALA
HHCCCCCEEEEECCCCCCHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9177243; 12060705 [H]