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The map label for this gene is fruK [H]

Identifier: 158318802

GI number: 158318802

Start: 8633198

End: 8634148

Strand: Reverse

Name: fruK [H]

Synonym: Franean1_7074

Alternate gene names: 158318802

Gene position: 8634148-8633198 (Counterclockwise)

Preceding gene: 158318803

Following gene: 158318801

Centisome position: 96.13

GC content: 72.66

Gene sequence:

>951_bases
ATGATCGTCACGGTGACGCTGAATCCCAGCCTCGATCGCACAATCGACATCAGCGCGCTGGCGCGTGGCGCCGTCATCCG
GGCGGCGTCGGCCCGGCTGGATCCGGCCGGTAAGGGTGTCAACGTGGCCCGGGCGCTGCTGGCCAACGGGAAGCGGTCGC
GCGCGGTGCTGTCGTGCGGCGGTGACGATGGCCAGCGACTGGTCAGGATGCTCGCCGCGGAGGGCGTCGACATGCTGGCG
GTGCCGATCGCCGGATCGACCCGGTCGAACATCACGTTGTCGGAGCCGGACGGCACCGTCACCAAGATCAACGAGCTGGG
TCCGTCGCTGTCCAAGGACGAGCTGGACACGGTCGCCGCCGCCGTGCTCGACTGCGACGTGCCGGGGGGATGGGTCGCGG
TGTGCGGCAGCCTTCCGCCTGGGGTGCCGGCGGATGCGTACGCGGACCTGTGCGTCCGGTTCCGCGCCGCGGGCCGAAGG
GTCGCGGTCGACACCAGCGGTGCGGCGCTCCTCGACGCGGTCGCCGCGGGGCCGGCGGTGGTGAAGCCCAACCAGGAGGA
GCTTGGTGAGGCGGTCGGCGCGCCGCTGCGCAACCTGGGTGAGGTGATTGAGGCGGCGCAGCAGCTTCGGACCTGGGGGG
CCGGCGCGGTCCTGGCCAGCCTGGGTGCGCACGGTGCGGTGCTTGTCGATGACCACGGCGCGGTGATGGGGGATTCTCCC
GTGCTCGGCCCGCGCAGCACCGTGGGCGCCGGGGACGCCCTCCTAGCAGGGTTCCTCGCGGCCGGTGCTCGCGGGCCCGA
GGCCTTGGCGGAAGCCTTGGCCTGGGGCGCCGCCGCCGTTAGCCTGCCGGGTAGCCGGATGCCCCGGGCCGAAGACATAC
GCCGGGATCGGGTCCGTATCCATAACCGCCCCGATTTCCACCGGGCCATGACCCTGCCGCTGCCCGCCTGA

Upstream 100 bases:

>100_bases
TCGCGACCCTCGCCGAGGTCGACATGTTCATCACGGACACCGGGCTCGACGCGCGGGTAGCCGCCGAGATCGAGGACGGC
GGGCCCCGCGTGGTACAGGC

Downstream 100 bases:

>100_bases
CCGCGTTCACCGCACCAACGGCAGTCCCTGATCCGGCTTTATCCCTGTCGAATTTTTCCCTATTTCTTGATATTTTCACC
GCCCCGCACTTGCGTCCGGG

Product: ribokinase-like domain-containing protein

Products: NA

Alternate protein names: Fructose 1-phosphate kinase [H]

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MIVTVTLNPSLDRTIDISALARGAVIRAASARLDPAGKGVNVARALLANGKRSRAVLSCGGDDGQRLVRMLAAEGVDMLA
VPIAGSTRSNITLSEPDGTVTKINELGPSLSKDELDTVAAAVLDCDVPGGWVAVCGSLPPGVPADAYADLCVRFRAAGRR
VAVDTSGAALLDAVAAGPAVVKPNQEELGEAVGAPLRNLGEVIEAAQQLRTWGAGAVLASLGAHGAVLVDDHGAVMGDSP
VLGPRSTVGAGDALLAGFLAAGARGPEALAEALAWGAAAVSLPGSRMPRAEDIRRDRVRIHNRPDFHRAMTLPLPA

Sequences:

>Translated_316_residues
MIVTVTLNPSLDRTIDISALARGAVIRAASARLDPAGKGVNVARALLANGKRSRAVLSCGGDDGQRLVRMLAAEGVDMLA
VPIAGSTRSNITLSEPDGTVTKINELGPSLSKDELDTVAAAVLDCDVPGGWVAVCGSLPPGVPADAYADLCVRFRAAGRR
VAVDTSGAALLDAVAAGPAVVKPNQEELGEAVGAPLRNLGEVIEAAQQLRTWGAGAVLASLGAHGAVLVDDHGAVMGDSP
VLGPRSTVGAGDALLAGFLAAGARGPEALAEALAWGAAAVSLPGSRMPRAEDIRRDRVRIHNRPDFHRAMTLPLPA
>Mature_316_residues
MIVTVTLNPSLDRTIDISALARGAVIRAASARLDPAGKGVNVARALLANGKRSRAVLSCGGDDGQRLVRMLAAEGVDMLA
VPIAGSTRSNITLSEPDGTVTKINELGPSLSKDELDTVAAAVLDCDVPGGWVAVCGSLPPGVPADAYADLCVRFRAAGRR
VAVDTSGAALLDAVAAGPAVVKPNQEELGEAVGAPLRNLGEVIEAAQQLRTWGAGAVLASLGAHGAVLVDDHGAVMGDSP
VLGPRSTVGAGDALLAGFLAAGARGPEALAEALAWGAAAVSLPGSRMPRAEDIRRDRVRIHNRPDFHRAMTLPLPA

Specific function: Key control step of glycolysis. [C]

COG id: COG1105

COG function: function code G; Fructose-1-phosphate kinase and related fructose-6-phosphate kinase (PfkB)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carbohydrate kinase pfkB family [H]

Homologues:

Organism=Escherichia coli, GI1788017, Length=300, Percent_Identity=34, Blast_Score=128, Evalue=5e-31,
Organism=Escherichia coli, GI1788493, Length=258, Percent_Identity=33.7209302325581, Blast_Score=127, Evalue=1e-30,

Paralogues:

None

Copy number: 496 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022463
- InterPro:   IPR011611
- InterPro:   IPR002173
- InterPro:   IPR017583 [H]

Pfam domain/function: PF00294 PfkB [H]

EC number: =2.7.1.56 [H]

Molecular weight: Translated: 32087; Mature: 32087

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS00583 PFKB_KINASES_1 ; PS00584 PFKB_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVTVTLNPSLDRTIDISALARGAVIRAASARLDPAGKGVNVARALLANGKRSRAVLSCG
CEEEEEECCCCCCEEEHHHHHCCHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCEEEEEC
GDDGQRLVRMLAAEGVDMLAVPIAGSTRSNITLSEPDGTVTKINELGPSLSKDELDTVAA
CCCHHHHHHHHHHCCCCEEEEECCCCCCCCEEEECCCCCEEEHHHCCCCCCHHHHHHHHH
AVLDCDVPGGWVAVCGSLPPGVPADAYADLCVRFRAAGRRVAVDTSGAALLDAVAAGPAV
HHHHCCCCCCHHEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHCCCEE
VKPNQEELGEAVGAPLRNLGEVIEAAQQLRTWGAGAVLASLGAHGAVLVDDHGAVMGDSP
ECCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEECCCCCEECCCC
VLGPRSTVGAGDALLAGFLAAGARGPEALAEALAWGAAAVSLPGSRMPRAEDIRRDRVRI
CCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCHHEEECCCCCCCCHHHHHHHHHEE
HNRPDFHRAMTLPLPA
CCCCCHHHEEECCCCC
>Mature Secondary Structure
MIVTVTLNPSLDRTIDISALARGAVIRAASARLDPAGKGVNVARALLANGKRSRAVLSCG
CEEEEEECCCCCCEEEHHHHHCCHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCEEEEEC
GDDGQRLVRMLAAEGVDMLAVPIAGSTRSNITLSEPDGTVTKINELGPSLSKDELDTVAA
CCCHHHHHHHHHHCCCCEEEEECCCCCCCCEEEECCCCCEEEHHHCCCCCCHHHHHHHHH
AVLDCDVPGGWVAVCGSLPPGVPADAYADLCVRFRAAGRRVAVDTSGAALLDAVAAGPAV
HHHHCCCCCCHHEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHCCCEE
VKPNQEELGEAVGAPLRNLGEVIEAAQQLRTWGAGAVLASLGAHGAVLVDDHGAVMGDSP
ECCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEECCCCCEECCCC
VLGPRSTVGAGDALLAGFLAAGARGPEALAEALAWGAAAVSLPGSRMPRAEDIRRDRVRI
CCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCHHEEECCCCCCCCHHHHHHHHHEE
HNRPDFHRAMTLPLPA
CCCCCHHHEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]