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The map label for this gene is ydiB [C]

Identifier: 158318786

GI number: 158318786

Start: 8612042

End: 8612860

Strand: Direct

Name: ydiB [C]

Synonym: Franean1_7058

Alternate gene names: 158318786

Gene position: 8612042-8612860 (Clockwise)

Preceding gene: 158318783

Following gene: 158318787

Centisome position: 95.88

GC content: 65.81

Gene sequence:

>819_bases
ATGGCGAGGATTATCGACAAGGACACCCGGCTGTGCATGTCGCTCGCCGGCCGGCCGGGGAACTTCGGCACCCGGTTCCA
CAACTTCCTGTACGACGAGCTCGGGCTGAACTTCGTCTACAAGGCCTTCACCACCGACGATCTGTGTTCGGCCATCGGCG
GTATCCGCGCACTGGGGATCCGGGGGTGCGCCGTTTCGATGCCGTTCAAGGAGGCCAGCATCGAGCTCGTCGACGAGCTG
GCCGACTCGGCCGCCGCGATACAATCCATCAACACGATAGTCAACGAAACTGGGCGGCTGCTGGCGTACAACACCGACTA
TATTGCCGTCTCCCGCCTGCTCGCCCAGTATCAGGTGTCACCGGACCACACCTTTGCGCTGCGGGGAAGCGGCGGTATGG
CGAAGGCCGTTGCCTGCGCCATACGCGATGCTGGGTTCCGGTCCGGCTGTGTCGTTGCCAGAAACGAGGCGACCGGCCGG
GCGCTGGCGGAGATGTATGGCTACGAGTGGCGGGCCGATCTGGGATCGCTGCGGCCGGCGCTGCTCGTCAACGTCACCCC
GATCGGGATGTCCGGCGGCCCGGAGGCGGCCGATCTCGCGTTCGAACTCGCCGCCGTCGACAAGGCCGAGGTTGTCTTCG
ACGTGGTCGCCGTTCCCGCCGAGACACCGCTCATCCGCTGTGCACGGGAGCGCGGCAGAACAGTGATCACCGGGGTCGAA
GTGATCGCGCTCCAGGCACTGGAGCAGTTCGTCCTGTACACCGGCGTCCGTCCGGACGACGACCTGGTACGCCGAGCGGC
TGCCTTCGCGAGGTCATAG

Upstream 100 bases:

>100_bases
CGACGTGGGACATCACAGAATGCGACTGCCACATTTCTTTTCCGACCCGTTTCTGTGTCACATGATCGCGACCGGCAAAG
GCACATGATAGGGTCGAAGC

Downstream 100 bases:

>100_bases
ATACGAGAGGGATTCGCGCGCCCTGGCCCAGTTCGGCATCCAGAGATCCTCCAAATACAAAATGAAGTTTTTCCGAAACT
CGCCACGGTCCACAGGTTTT

Product: shikimate dehydrogenase substrate binding subunit

Products: 3-dehydroshikimate; NADPH; H+

Alternate protein names: SDH-L [H]

Number of amino acids: Translated: 272; Mature: 271

Protein sequence:

>272_residues
MARIIDKDTRLCMSLAGRPGNFGTRFHNFLYDELGLNFVYKAFTTDDLCSAIGGIRALGIRGCAVSMPFKEASIELVDEL
ADSAAAIQSINTIVNETGRLLAYNTDYIAVSRLLAQYQVSPDHTFALRGSGGMAKAVACAIRDAGFRSGCVVARNEATGR
ALAEMYGYEWRADLGSLRPALLVNVTPIGMSGGPEAADLAFELAAVDKAEVVFDVVAVPAETPLIRCARERGRTVITGVE
VIALQALEQFVLYTGVRPDDDLVRRAAAFARS

Sequences:

>Translated_272_residues
MARIIDKDTRLCMSLAGRPGNFGTRFHNFLYDELGLNFVYKAFTTDDLCSAIGGIRALGIRGCAVSMPFKEASIELVDEL
ADSAAAIQSINTIVNETGRLLAYNTDYIAVSRLLAQYQVSPDHTFALRGSGGMAKAVACAIRDAGFRSGCVVARNEATGR
ALAEMYGYEWRADLGSLRPALLVNVTPIGMSGGPEAADLAFELAAVDKAEVVFDVVAVPAETPLIRCARERGRTVITGVE
VIALQALEQFVLYTGVRPDDDLVRRAAAFARS
>Mature_271_residues
ARIIDKDTRLCMSLAGRPGNFGTRFHNFLYDELGLNFVYKAFTTDDLCSAIGGIRALGIRGCAVSMPFKEASIELVDELA
DSAAAIQSINTIVNETGRLLAYNTDYIAVSRLLAQYQVSPDHTFALRGSGGMAKAVACAIRDAGFRSGCVVARNEATGRA
LAEMYGYEWRADLGSLRPALLVNVTPIGMSGGPEAADLAFELAAVDKAEVVFDVVAVPAETPLIRCARERGRTVITGVEV
IALQALEQFVLYTGVRPDDDLVRRAAAFARS

Specific function: The physiological substrate is not known. Has much lower activity towards shikimate than AroE [H]

COG id: COG0169

COG function: function code E; Shikimate 5-dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the shikimate dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI1787983, Length=266, Percent_Identity=31.203007518797, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1789675, Length=227, Percent_Identity=26.431718061674, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR013708
- InterPro:   IPR006151 [H]

Pfam domain/function: PF01488 Shikimate_DH; PF08501 Shikimate_dh_N [H]

EC number: 1.1.1.25

Molecular weight: Translated: 29204; Mature: 29073

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARIIDKDTRLCMSLAGRPGNFGTRFHNFLYDELGLNFVYKAFTTDDLCSAIGGIRALGI
CCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCHHHEEECCCHHHHHHHHHHHHHHCC
RGCAVSMPFKEASIELVDELADSAAAIQSINTIVNETGRLLAYNTDYIAVSRLLAQYQVS
CCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHCCC
PDHTFALRGSGGMAKAVACAIRDAGFRSGCVVARNEATGRALAEMYGYEWRADLGSLRPA
CCCEEEEECCCCHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHCCCHHHHHHCCCCE
LLVNVTPIGMSGGPEAADLAFELAAVDKAEVVFDVVAVPAETPLIRCARERGRTVITGVE
EEEEEEECCCCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHCCCEEEEHHH
VIALQALEQFVLYTGVRPDDDLVRRAAAFARS
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
ARIIDKDTRLCMSLAGRPGNFGTRFHNFLYDELGLNFVYKAFTTDDLCSAIGGIRALGI
CCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCHHHEEECCCHHHHHHHHHHHHHHCC
RGCAVSMPFKEASIELVDELADSAAAIQSINTIVNETGRLLAYNTDYIAVSRLLAQYQVS
CCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHCCC
PDHTFALRGSGGMAKAVACAIRDAGFRSGCVVARNEATGRALAEMYGYEWRADLGSLRPA
CCCEEEEECCCCHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHCCCHHHHHHCCCCE
LLVNVTPIGMSGGPEAADLAFELAAVDKAEVVFDVVAVPAETPLIRCARERGRTVITGVE
EEEEEEECCCCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHCCCEEEEHHH
VIALQALEQFVLYTGVRPDDDLVRRAAAFARS
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: shikimate; NADP+

Specific reaction: shikimate + NADP+ = 3-dehydroshikimate + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]