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The map label for this gene is ppsA [H]

Identifier: 158318673

GI number: 158318673

Start: 8457092

End: 8459524

Strand: Reverse

Name: ppsA [H]

Synonym: Franean1_6941

Alternate gene names: 158318673

Gene position: 8459524-8457092 (Counterclockwise)

Preceding gene: 158318674

Following gene: 158318671

Centisome position: 94.18

GC content: 72.09

Gene sequence:

>2433_bases
GTGAGCGAGCGTCGGTGGATCGCGTGGTTCAAGGAGATCGGCGCGGACGACGTCGACCAGGCCGGGGGCAAGGGCGCGAA
CCTCGGAGAGCTGACCCGCGCCGGGCTGCCCGTGCCACCGGGATTCGTGATCACGGTGTCCGCGTACCTGGACGTCGTCG
ACGCGGCCTGGATGCGCTCGGAGATCGCCGCCCGCGCCTCGGACATCGACCCTGACGACCATGCGCAGCTCGAACAGGTC
GCCGGGGAGTTACGGGCGCACATAGTCGCGGCGCCCGTCCCGGAGTCGTTGGGCCGCGCGATCTCGGAGGCATACCGGGA
ACTGGGCGGTGGCTCGGTGGCAGTGCGGTCGTCGGCGACGGCCGAGGACGCGGCCGGGACGTCCTTCGCGGGCATGAACA
GCACGTTCACCAACGTCTCCGGCGAGACGGAACTCCTCGCGCGGGTTCGCGAGTGCTGGGCCTCGCTGTATGGGCCGCGA
GCCGTCGCCTACCGGGCCAGCCGCCACATGGTGGCCGAACCGGAGACCGCGGTCGTGGTCCAGCGGATGGTCGACGCGGA
GCGGTCGGGCGTGATGTTCTCGGTCGACCCGATCACCGGTGACAGGTCCCGCATCGTGATCGAAGCGGCCTTCGGGCTCG
GCGAGGTCGTCGTCGGCGGCGAGGTCATCCCGGACACCTACGTCGTGGACAAGGACGGCCCGCGGCTGGTCGACGTCCGG
ACCGGCGCGCAGACCCATCAGATCATCCGTGGCGCAGACGGCCACGACCTGCTCGTGGACCTGCTTCCGACGGAGGGACG
CCACCGGATTCTCACCGAGGACGAGGTACTGGACCTGGCGCGGCTGGCTCTGCGCGTCGAGGCGCACTACGGCGAGCCGC
AGGACGTCGAATGGGCGATCGAGGGACACCGGACACATCTCGTGCAGTCCCGCCCCGTCACGACCGGCGGGGCGGCACCG
GTCACGACCGCACCCCGGCCCACGAGAATCGAGTCGTCCGAGAGGACCGGCCCGTCCGAGAGGACCGGCAGGAGCGAGCC
CTCCGACACTGTGCTGCTCACCGGCCTCGGGGGCGCGCCGGGGCGGGCGGCCGGCCGGGTCCGCGTCCTCACCTCACCCG
GCGAGGGCGCGGCGTTCGCGACCGGGGAGGTCCTGGTCGCCGCGATGACCTCCCCGGACTGGGTGCCCGTCATCCGCCGC
GCCGCCGCGGTGGTCACCGACAGCGGCGGGGTGACCTGCCATGCCGCGATCGTCGCCCGCGAGCTGGGCGTGCCCTGCGT
GGTCGGGACGAGCAGCGCGACATCCACGCTGCGCACCGGCGACACGGTCGTCGTCGACGGCAGCCGCGGGGTGGTCGAGA
AGGACGCGGACATGTGGGAGACCCCGCGGGCACTGGCCAGTCCGGGGCGAGCCATGGTGAAGGCCATCCCGTCACCCGCG
GTCGCGATCGAGGTCGCGCCCGTCGTCCCGGCGGGCCAGGCGCCGAACCCGGTGGTTCCGGTGGCTGTCGAGCCGACCGC
CACCAGGCTCTATGTGAATCTTGCCGTCGCCGACCACGTGGAGCAGGTCGCGGCACTGCCTGTCGCGGGCGTGGGCCTGC
TGCGGGCGGAGCTCCTCCTGGTCGACGCGCTTGGTGGGACGCATCCCCGCCATCTGCTGGAGCAGGGCGGCCGTGCGGAG
TTCGTGGAACGGATGGCCGCCTCCGTCGAGCGGATCACCCGCGCGTTCGCGCCCCGGCCGGTGATCTACCGGGCGACGGA
CTTCCGCACCAACGAGTTCCGCGGGCTTACCGGTGGCGACCGTTTCGAACCGGTCGAGGCGAACCCGATGATCGGCTACC
GGGGCTGCTTCCGCTACGTCCGTGATCCCGCCGTGTTCGACGCCGAGCTGGAGGTGCTGGCCGTCGTGCGGGAACGCACG
CCGAACCTGCACCTGATGATTCCGTTCGTGCGGACCGGCTGGGAGCTCGAGGCCTGCCTGAAGGCGGTGGACGCCAGTCC
GCTCGGTGACCATCGGGACATGCTCCGCTGGGTGATGGCCGAGGTGCCCTCGGCCGCCTACTGGATCCCCGCCTACGCCC
GGCTCGGGATCGACGGGGTCTCCATCGGCTCCAACGACCTGACCCAGCTCGTGCTCGGTGTCGACCGGGACTCCGAGACC
TGCGCCGAGCTGTTCGACGAGGCGGACCCGGCGGTGCTCGACACCATCGCCCGCATCATCACGGCATGCCGGGAGAACGG
TCTCACCTCGTCGCTGTGCGGGCAGGCCCCCTCGACCAGGCCCGACTACGCCGAGCGGCTCGTCGAGCTCGGCATCACCT
CGGTCTCGGTCGATCCAGACGCGGTGGGTGCCGCGGCGTGGTCGATCGCCGCCGCGGAGCGCCGCATCCTCCTCGACGCG
GCCCGGACCGGGATGGCGTCAGGGCGGGGCTGA

Upstream 100 bases:

>100_bases
TGCTGCTGCGGCGGCTCGTGCGCGACGTCCCCGGGGTGCTGGGCCTGGAGATCGCACAACCGGTCGGCGTGGCCGGCGCG
AGGGGCGAGGAGGTCGACCA

Downstream 100 bases:

>100_bases
CCTCGGAGGCCAGCGGTGATCGCGGGCCCGGGGAAGCGATGCGAGCGGGGGGATCAGGCCGGCAGCTCCGATCCTTCCGC
CCGCCCGGCATCCCTTCCGT

Product: pyruvate phosphate dikinase

Products: NA

Alternate protein names: PEP synthase; Pyruvate, water dikinase [H]

Number of amino acids: Translated: 810; Mature: 809

Protein sequence:

>810_residues
MSERRWIAWFKEIGADDVDQAGGKGANLGELTRAGLPVPPGFVITVSAYLDVVDAAWMRSEIAARASDIDPDDHAQLEQV
AGELRAHIVAAPVPESLGRAISEAYRELGGGSVAVRSSATAEDAAGTSFAGMNSTFTNVSGETELLARVRECWASLYGPR
AVAYRASRHMVAEPETAVVVQRMVDAERSGVMFSVDPITGDRSRIVIEAAFGLGEVVVGGEVIPDTYVVDKDGPRLVDVR
TGAQTHQIIRGADGHDLLVDLLPTEGRHRILTEDEVLDLARLALRVEAHYGEPQDVEWAIEGHRTHLVQSRPVTTGGAAP
VTTAPRPTRIESSERTGPSERTGRSEPSDTVLLTGLGGAPGRAAGRVRVLTSPGEGAAFATGEVLVAAMTSPDWVPVIRR
AAAVVTDSGGVTCHAAIVARELGVPCVVGTSSATSTLRTGDTVVVDGSRGVVEKDADMWETPRALASPGRAMVKAIPSPA
VAIEVAPVVPAGQAPNPVVPVAVEPTATRLYVNLAVADHVEQVAALPVAGVGLLRAELLLVDALGGTHPRHLLEQGGRAE
FVERMAASVERITRAFAPRPVIYRATDFRTNEFRGLTGGDRFEPVEANPMIGYRGCFRYVRDPAVFDAELEVLAVVRERT
PNLHLMIPFVRTGWELEACLKAVDASPLGDHRDMLRWVMAEVPSAAYWIPAYARLGIDGVSIGSNDLTQLVLGVDRDSET
CAELFDEADPAVLDTIARIITACRENGLTSSLCGQAPSTRPDYAERLVELGITSVSVDPDAVGAAAWSIAAAERRILLDA
ARTGMASGRG

Sequences:

>Translated_810_residues
MSERRWIAWFKEIGADDVDQAGGKGANLGELTRAGLPVPPGFVITVSAYLDVVDAAWMRSEIAARASDIDPDDHAQLEQV
AGELRAHIVAAPVPESLGRAISEAYRELGGGSVAVRSSATAEDAAGTSFAGMNSTFTNVSGETELLARVRECWASLYGPR
AVAYRASRHMVAEPETAVVVQRMVDAERSGVMFSVDPITGDRSRIVIEAAFGLGEVVVGGEVIPDTYVVDKDGPRLVDVR
TGAQTHQIIRGADGHDLLVDLLPTEGRHRILTEDEVLDLARLALRVEAHYGEPQDVEWAIEGHRTHLVQSRPVTTGGAAP
VTTAPRPTRIESSERTGPSERTGRSEPSDTVLLTGLGGAPGRAAGRVRVLTSPGEGAAFATGEVLVAAMTSPDWVPVIRR
AAAVVTDSGGVTCHAAIVARELGVPCVVGTSSATSTLRTGDTVVVDGSRGVVEKDADMWETPRALASPGRAMVKAIPSPA
VAIEVAPVVPAGQAPNPVVPVAVEPTATRLYVNLAVADHVEQVAALPVAGVGLLRAELLLVDALGGTHPRHLLEQGGRAE
FVERMAASVERITRAFAPRPVIYRATDFRTNEFRGLTGGDRFEPVEANPMIGYRGCFRYVRDPAVFDAELEVLAVVRERT
PNLHLMIPFVRTGWELEACLKAVDASPLGDHRDMLRWVMAEVPSAAYWIPAYARLGIDGVSIGSNDLTQLVLGVDRDSET
CAELFDEADPAVLDTIARIITACRENGLTSSLCGQAPSTRPDYAERLVELGITSVSVDPDAVGAAAWSIAAAERRILLDA
ARTGMASGRG
>Mature_809_residues
SERRWIAWFKEIGADDVDQAGGKGANLGELTRAGLPVPPGFVITVSAYLDVVDAAWMRSEIAARASDIDPDDHAQLEQVA
GELRAHIVAAPVPESLGRAISEAYRELGGGSVAVRSSATAEDAAGTSFAGMNSTFTNVSGETELLARVRECWASLYGPRA
VAYRASRHMVAEPETAVVVQRMVDAERSGVMFSVDPITGDRSRIVIEAAFGLGEVVVGGEVIPDTYVVDKDGPRLVDVRT
GAQTHQIIRGADGHDLLVDLLPTEGRHRILTEDEVLDLARLALRVEAHYGEPQDVEWAIEGHRTHLVQSRPVTTGGAAPV
TTAPRPTRIESSERTGPSERTGRSEPSDTVLLTGLGGAPGRAAGRVRVLTSPGEGAAFATGEVLVAAMTSPDWVPVIRRA
AAVVTDSGGVTCHAAIVARELGVPCVVGTSSATSTLRTGDTVVVDGSRGVVEKDADMWETPRALASPGRAMVKAIPSPAV
AIEVAPVVPAGQAPNPVVPVAVEPTATRLYVNLAVADHVEQVAALPVAGVGLLRAELLLVDALGGTHPRHLLEQGGRAEF
VERMAASVERITRAFAPRPVIYRATDFRTNEFRGLTGGDRFEPVEANPMIGYRGCFRYVRDPAVFDAELEVLAVVRERTP
NLHLMIPFVRTGWELEACLKAVDASPLGDHRDMLRWVMAEVPSAAYWIPAYARLGIDGVSIGSNDLTQLVLGVDRDSETC
AELFDEADPAVLDTIARIITACRENGLTSSLCGQAPSTRPDYAERLVELGITSVSVDPDAVGAAAWSIAAAERRILLDAA
RTGMASGRG

Specific function: Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=842, Percent_Identity=35.3919239904988, Blast_Score=430, Evalue=1e-121,
Organism=Escherichia coli, GI1789193, Length=412, Percent_Identity=25.4854368932039, Blast_Score=112, Evalue=1e-25,
Organism=Escherichia coli, GI1788756, Length=394, Percent_Identity=25.6345177664975, Blast_Score=99, Evalue=8e-22,
Organism=Escherichia coli, GI48994992, Length=370, Percent_Identity=27.027027027027, Blast_Score=93, Evalue=8e-20,
Organism=Escherichia coli, GI1788726, Length=239, Percent_Identity=27.6150627615063, Blast_Score=84, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17564524, Length=308, Percent_Identity=28.2467532467532, Blast_Score=113, Evalue=5e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR006319
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.2 [H]

Molecular weight: Translated: 85884; Mature: 85753

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSERRWIAWFKEIGADDVDQAGGKGANLGELTRAGLPVPPGFVITVSAYLDVVDAAWMRS
CCCCHHHHHHHHCCCCCHHHCCCCCCCHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHH
EIAARASDIDPDDHAQLEQVAGELRAHIVAAPVPESLGRAISEAYRELGGGSVAVRSSAT
HHHHHHCCCCCCHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHCCCEEEEECCCC
AEDAAGTSFAGMNSTFTNVSGETELLARVRECWASLYGPRAVAYRASRHMVAEPETAVVV
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHEEEHHCCCCCCCCHHHHHH
QRMVDAERSGVMFSVDPITGDRSRIVIEAAFGLGEVVVGGEVIPDTYVVDKDGPRLVDVR
HHHHHHHHCCEEEEECCCCCCCCEEEEEEECCCCEEEECCEECCCCEEEECCCCEEEEEE
TGAQTHQIIRGADGHDLLVDLLPTEGRHRILTEDEVLDLARLALRVEAHYGEPQDVEWAI
CCCHHHHHHCCCCCCEEEEEECCCCCCCEECCHHHHHHHHHHHHHHEECCCCCCCCEEEE
EGHRTHLVQSRPVTTGGAAPVTTAPRPTRIESSERTGPSERTGRSEPSDTVLLTGLGGAP
CCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCC
GRAAGRVRVLTSPGEGAAFATGEVLVAAMTSPDWVPVIRRAAAVVTDSGGVTCHAAIVAR
CCCCCEEEEEECCCCCCEEECCCEEEEEECCCCHHHHHHHHHHEEECCCCCEEHHHHHHH
ELGVPCVVGTSSATSTLRTGDTVVVDGSRGVVEKDADMWETPRALASPGRAMVKAIPSPA
HCCCCEEEECCCCCCCEECCCEEEEECCCCCCCCCCHHHCCCHHHHCCCHHHHHHCCCCC
VAIEVAPVVPAGQAPNPVVPVAVEPTATRLYVNLAVADHVEQVAALPVAGVGLLRAELLL
EEEEEECCCCCCCCCCCEEEEEECCCCEEEEEEEHHHHHHHHHHHCCHHHHHHHHHHHHE
VDALGGTHPRHLLEQGGRAEFVERMAASVERITRAFAPRPVIYRATDFRTNEFRGLTGGD
EECCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCC
RFEPVEANPMIGYRGCFRYVRDPAVFDAELEVLAVVRERTPNLHLMIPFVRTGWELEACL
CCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEEEEEHHHCCCCHHHHH
KAVDASPLGDHRDMLRWVMAEVPSAAYWIPAYARLGIDGVSIGSNDLTQLVLGVDRDSET
HHHCCCCCCCHHHHHHHHHHHCCCCEEEHHHHHHCCCCCEECCCHHHHHHHEECCCCHHH
CAELFDEADPAVLDTIARIITACRENGLTSSLCGQAPSTRPDYAERLVELGITSVSVDPD
HHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHHHHHHCCCEEECCCC
AVGAAAWSIAAAERRILLDAARTGMASGRG
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SERRWIAWFKEIGADDVDQAGGKGANLGELTRAGLPVPPGFVITVSAYLDVVDAAWMRS
CCCHHHHHHHHCCCCCHHHCCCCCCCHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHH
EIAARASDIDPDDHAQLEQVAGELRAHIVAAPVPESLGRAISEAYRELGGGSVAVRSSAT
HHHHHHCCCCCCHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHCCCEEEEECCCC
AEDAAGTSFAGMNSTFTNVSGETELLARVRECWASLYGPRAVAYRASRHMVAEPETAVVV
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHEEEHHCCCCCCCCHHHHHH
QRMVDAERSGVMFSVDPITGDRSRIVIEAAFGLGEVVVGGEVIPDTYVVDKDGPRLVDVR
HHHHHHHHCCEEEEECCCCCCCCEEEEEEECCCCEEEECCEECCCCEEEECCCCEEEEEE
TGAQTHQIIRGADGHDLLVDLLPTEGRHRILTEDEVLDLARLALRVEAHYGEPQDVEWAI
CCCHHHHHHCCCCCCEEEEEECCCCCCCEECCHHHHHHHHHHHHHHEECCCCCCCCEEEE
EGHRTHLVQSRPVTTGGAAPVTTAPRPTRIESSERTGPSERTGRSEPSDTVLLTGLGGAP
CCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCC
GRAAGRVRVLTSPGEGAAFATGEVLVAAMTSPDWVPVIRRAAAVVTDSGGVTCHAAIVAR
CCCCCEEEEEECCCCCCEEECCCEEEEEECCCCHHHHHHHHHHEEECCCCCEEHHHHHHH
ELGVPCVVGTSSATSTLRTGDTVVVDGSRGVVEKDADMWETPRALASPGRAMVKAIPSPA
HCCCCEEEECCCCCCCEECCCEEEEECCCCCCCCCCHHHCCCHHHHCCCHHHHHHCCCCC
VAIEVAPVVPAGQAPNPVVPVAVEPTATRLYVNLAVADHVEQVAALPVAGVGLLRAELLL
EEEEEECCCCCCCCCCCEEEEEECCCCEEEEEEEHHHHHHHHHHHCCHHHHHHHHHHHHE
VDALGGTHPRHLLEQGGRAEFVERMAASVERITRAFAPRPVIYRATDFRTNEFRGLTGGD
EECCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCC
RFEPVEANPMIGYRGCFRYVRDPAVFDAELEVLAVVRERTPNLHLMIPFVRTGWELEACL
CCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEEEEEHHHCCCCHHHHH
KAVDASPLGDHRDMLRWVMAEVPSAAYWIPAYARLGIDGVSIGSNDLTQLVLGVDRDSET
HHHCCCCCCCHHHHHHHHHHHCCCCEEEHHHHHHCCCCCEECCCHHHHHHHEECCCCHHH
CAELFDEADPAVLDTIARIITACRENGLTSSLCGQAPSTRPDYAERLVELGITSVSVDPD
HHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHHHHHHCCCEEECCCC
AVGAAAWSIAAAERRILLDAARTGMASGRG
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7805870 [H]