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The map label for this gene is fadE25 [H]

Identifier: 158318656

GI number: 158318656

Start: 8433376

End: 8434473

Strand: Reverse

Name: fadE25 [H]

Synonym: Franean1_6924

Alternate gene names: 158318656

Gene position: 8434473-8433376 (Counterclockwise)

Preceding gene: 158318657

Following gene: 158318655

Centisome position: 93.9

GC content: 69.58

Gene sequence:

>1098_bases
ATGGACGTCGCGGACTTCCGGTCGGATCTCCAGGCGTGGCTTGCCGAGAACGACCTGACCCCAGGACCCGACCATTCCCT
TGACGGGCAGGTGGCTCAGCTCGCGCGGGTGCGTCGCGCCCTGTACGACGCGGGCTGGATGCGTCACGGCTGGCCCGCCG
AGGTGGGTGGCCTCGGTGGCCCGGCGGTGCTGCGCGCCGTACTTGGCGAGGAGGTGGCCAGCCGCGGTCTGGCCGAACCG
GGCATCTACTCGATGGTCGAGGTACTTGTCCCGACCATGATCTCCTATGCGTCGCCGGCGCTGGCGGCCGAGATGGTGCC
GCTGCTGCTGTCCGGTACCGAGAGCTGGTGCCAGGGGTTCTCCGAGCCGGGGTCGGGCAGTGACCTGGCGTCGCTGTCCA
CCCGGGCGGAGCCCCGTGGGGACGACTGGGTGGTCAACGGGCAGAAGGTCTGGACGAGCCTGGCCCAGTACTCCGAGCGC
TGCGTGCTGCTCACCCGCACCGCACCGGGCCACGGCGGGATCACGGCGTTCTTCGTTGACATGGACACCCCCGGCATCAC
CGTCCGTCCGCTGAAAATGATGCACGGTGTCGAGGAGTTCGCTGAGGTCTTCTTCGACGACGTGGTCGTCCCCGGCGACC
GCATGCTCGGAAAGCCCGGCGACGGCTGGCAGATGGCGATGGACCTGCTCCCGCACGAGCGCTCCACCTGCTTCTGGCAC
CGGGTCGCCCACCTGTTCACCCGGCTCGACCGGCTGGTGACCGAGACGACCGCGCCGGGTGATCAGGCGGACGCGGATCT
GGGTGCGGCCTACCTGGCGCTGCACACCATCCGCTGCCGCTCGCACGCCACCCAGCGCCACCTCGGTGAAGGCGCCCACC
TCGGGCCGGAGACGTCGATCGACAAGGTGCTGCTCGCCACCGCCGAGCAACGCCTGTACGACACCGCCCGCGACCTGCTC
CCCGGCACCATCGAACTCACCGACACCCGCTGGCGCTCCGAGTACCTCTACTCCCGCGCCGCCACCATCTACGGCGGAAC
CGCCGAGATCCAGCGGAACATCATCGCCCGCCGACTCCTCGACCTCGGCAAGGAATAA

Upstream 100 bases:

>100_bases
GCCGCCGGGTCAGGGAGGACCGCGATGCCGATACCTGCCGAGTTCGCAGACCGACAGCCCGTAACGGTAGCTGCCGTGTT
CCGCGCCGAGGTCGCGTGAG

Downstream 100 bases:

>100_bases
CCATGGACGCCGCCGAACGCGAACTCCTCACCGAGACCTTCCGCAAGGCCATGACCGGCACCACCGGCGCCGCCCTCGAC
ACCGCCCTCACCGACCTCGG

Product: acyl-CoA dehydrogenase domain-containing protein

Products: gamma-butyrobetaine; Acceptor; gamma-butyrobetainyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MDVADFRSDLQAWLAENDLTPGPDHSLDGQVAQLARVRRALYDAGWMRHGWPAEVGGLGGPAVLRAVLGEEVASRGLAEP
GIYSMVEVLVPTMISYASPALAAEMVPLLLSGTESWCQGFSEPGSGSDLASLSTRAEPRGDDWVVNGQKVWTSLAQYSER
CVLLTRTAPGHGGITAFFVDMDTPGITVRPLKMMHGVEEFAEVFFDDVVVPGDRMLGKPGDGWQMAMDLLPHERSTCFWH
RVAHLFTRLDRLVTETTAPGDQADADLGAAYLALHTIRCRSHATQRHLGEGAHLGPETSIDKVLLATAEQRLYDTARDLL
PGTIELTDTRWRSEYLYSRAATIYGGTAEIQRNIIARRLLDLGKE

Sequences:

>Translated_365_residues
MDVADFRSDLQAWLAENDLTPGPDHSLDGQVAQLARVRRALYDAGWMRHGWPAEVGGLGGPAVLRAVLGEEVASRGLAEP
GIYSMVEVLVPTMISYASPALAAEMVPLLLSGTESWCQGFSEPGSGSDLASLSTRAEPRGDDWVVNGQKVWTSLAQYSER
CVLLTRTAPGHGGITAFFVDMDTPGITVRPLKMMHGVEEFAEVFFDDVVVPGDRMLGKPGDGWQMAMDLLPHERSTCFWH
RVAHLFTRLDRLVTETTAPGDQADADLGAAYLALHTIRCRSHATQRHLGEGAHLGPETSIDKVLLATAEQRLYDTARDLL
PGTIELTDTRWRSEYLYSRAATIYGGTAEIQRNIIARRLLDLGKE
>Mature_365_residues
MDVADFRSDLQAWLAENDLTPGPDHSLDGQVAQLARVRRALYDAGWMRHGWPAEVGGLGGPAVLRAVLGEEVASRGLAEP
GIYSMVEVLVPTMISYASPALAAEMVPLLLSGTESWCQGFSEPGSGSDLASLSTRAEPRGDDWVVNGQKVWTSLAQYSER
CVLLTRTAPGHGGITAFFVDMDTPGITVRPLKMMHGVEEFAEVFFDDVVVPGDRMLGKPGDGWQMAMDLLPHERSTCFWH
RVAHLFTRLDRLVTETTAPGDQADADLGAAYLALHTIRCRSHATQRHLGEGAHLGPETSIDKVLLATAEQRLYDTARDLL
PGTIELTDTRWRSEYLYSRAATIYGGTAEIQRNIIARRLLDLGKE

Specific function: Catalyzes the reduction of crotonobetainyl-CoA to gamma-butyrobetainyl-CoA. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acyl-CoA dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI4557233, Length=362, Percent_Identity=29.0055248618785, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI4501857, Length=378, Percent_Identity=25.3968253968254, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI4501859, Length=356, Percent_Identity=25.2808988764045, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI4557231, Length=353, Percent_Identity=27.1954674220963, Blast_Score=89, Evalue=8e-18,
Organism=Homo sapiens, GI187960098, Length=353, Percent_Identity=27.1954674220963, Blast_Score=89, Evalue=8e-18,
Organism=Homo sapiens, GI21361497, Length=327, Percent_Identity=25.9938837920489, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI76496475, Length=340, Percent_Identity=25.2941176470588, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI4557235, Length=198, Percent_Identity=31.8181818181818, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI226958412, Length=238, Percent_Identity=25.2100840336134, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI226958414, Length=191, Percent_Identity=28.2722513089005, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI7656849, Length=180, Percent_Identity=31.6666666666667, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1786223, Length=202, Percent_Identity=28.7128712871287, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17570075, Length=379, Percent_Identity=25.8575197889182, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17569725, Length=332, Percent_Identity=25.6024096385542, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17534899, Length=325, Percent_Identity=27.3846153846154, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17508101, Length=341, Percent_Identity=25.2199413489736, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17538396, Length=229, Percent_Identity=29.2576419213974, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI71990804, Length=203, Percent_Identity=30.0492610837438, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17506239, Length=207, Percent_Identity=28.5024154589372, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71985184, Length=302, Percent_Identity=24.8344370860927, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI71985192, Length=171, Percent_Identity=28.0701754385965, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI32563615, Length=192, Percent_Identity=29.1666666666667, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17505929, Length=205, Percent_Identity=26.8292682926829, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17551932, Length=234, Percent_Identity=22.6495726495727, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI21356377, Length=365, Percent_Identity=27.3972602739726, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24646207, Length=371, Percent_Identity=26.1455525606469, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24666513, Length=340, Percent_Identity=25, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24660351, Length=367, Percent_Identity=23.9782016348774, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI21355753, Length=331, Percent_Identity=26.2839879154079, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI281363737, Length=348, Percent_Identity=25.2873563218391, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006089
- InterPro:   IPR006092
- InterPro:   IPR006090
- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR013786
- InterPro:   IPR009100 [H]

Pfam domain/function: PF00441 Acyl-CoA_dh_1; PF02770 Acyl-CoA_dh_M; PF02771 Acyl-CoA_dh_N [H]

EC number: 1.3.99.- [C]

Molecular weight: Translated: 39939; Mature: 39939

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVADFRSDLQAWLAENDLTPGPDHSLDGQVAQLARVRRALYDAGWMRHGWPAEVGGLGG
CCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCH
PAVLRAVLGEEVASRGLAEPGIYSMVEVLVPTMISYASPALAAEMVPLLLSGTESWCQGF
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHCCC
SEPGSGSDLASLSTRAEPRGDDWVVNGQKVWTSLAQYSERCVLLTRTAPGHGGITAFFVD
CCCCCCCCHHHHHHCCCCCCCCEEECHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEEE
MDTPGITVRPLKMMHGVEEFAEVFFDDVVVPGDRMLGKPGDGWQMAMDLLPHERSTCFWH
CCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCCHHHHHHHHCCCCCHHHHHH
RVAHLFTRLDRLVTETTAPGDQADADLGAAYLALHTIRCRSHATQRHLGEGAHLGPETSI
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHH
DKVLLATAEQRLYDTARDLLPGTIELTDTRWRSEYLYSRAATIYGGTAEIQRNIIARRLL
HHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
DLGKE
HHCCC
>Mature Secondary Structure
MDVADFRSDLQAWLAENDLTPGPDHSLDGQVAQLARVRRALYDAGWMRHGWPAEVGGLGG
CCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCH
PAVLRAVLGEEVASRGLAEPGIYSMVEVLVPTMISYASPALAAEMVPLLLSGTESWCQGF
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHCCC
SEPGSGSDLASLSTRAEPRGDDWVVNGQKVWTSLAQYSERCVLLTRTAPGHGGITAFFVD
CCCCCCCCHHHHHHCCCCCCCCEEECHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEEE
MDTPGITVRPLKMMHGVEEFAEVFFDDVVVPGDRMLGKPGDGWQMAMDLLPHERSTCFWH
CCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCCHHHHHHHHCCCCCHHHHHH
RVAHLFTRLDRLVTETTAPGDQADADLGAAYLALHTIRCRSHATQRHLGEGAHLGPETSI
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHH
DKVLLATAEQRLYDTARDLLPGTIELTDTRWRSEYLYSRAATIYGGTAEIQRNIIARRLL
HHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
DLGKE
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: crotono-betaine; reduced acceptor; crotonobetainyl-CoA [C]

Specific reaction: reduced acceptor + crotono-betaine = Acceptor + gamma-butyrobetaine reduced acceptor + crotonobetainyl-CoA = Acceptor + gamma-butyrobetainyl-CoA [C]

General reaction: Oxidoreductases; Acting on the CH-CH group of donors; With other acceptors [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]