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The map label for this gene is nifN [H]

Identifier: 158318609

GI number: 158318609

Start: 8379773

End: 8381242

Strand: Reverse

Name: nifN [H]

Synonym: Franean1_6877

Alternate gene names: 158318609

Gene position: 8381242-8379773 (Counterclockwise)

Preceding gene: 158318610

Following gene: 158318608

Centisome position: 93.31

GC content: 73.88

Gene sequence:

>1470_bases
ATGGCGAGGGTCGTCACGAGTGACCGCCGGCCCGGCCTGGACCCGCTGCGGTTCAGCCAGCCGCTCGGCGGGGCACTGGT
CTTCCTCGGCCTCGCCGCGGCGATGCCCGTCATGCACGGGTCGAAGGGCTGCGCCTCGTTCGCCAAGGCGCTGCTGACCC
GGCACTTCAACGAGCCCGTCCCGCTGCAGACCACCGGTGTCACCGAGGTGTCCGCGGTGCTCGGCAGTGGCGACGACCTC
GTCGCCAACCTGGACGGCATCCGCGCCAAGCAGAACCCGCGGATCATCGGGCTGCTGACCACCGGCGTCACCGAGGTCAG
CGGCGAGGACGTCGCCGGCCAGGTCCGCCAGTACATCGCGATGATGAACCACACCACCCCCGAGGGCGCGCCGCTGATCG
TCCGGGTGTCCACGCCGGACTTCGCCGGCGGGCTGTCGGACGGCTGGTCGGCCGCGCTGCGCTCGCTGGTCGCCACCGTC
CCCTTCGACCACGCCGACTCGGACGAGTACCCGGGTACGCGCTCGGGCTTCGGCGCCGGAACCGGTTCCGCGCCCGAGAC
GGTCGCCGTGCTCGTCGGCCCGTCCCTGTCGGCCGCCGACCTCGACGAGCTCTGCGCGCTGATCCGTTCCTTCGGGATGG
CGCCGGTGCTGGTCCCGGATCTCTCCGGCTCCCTCGACGGGCACCTGGCCCCGTCCTGGCAGCCGACGACGACCGGTGGC
ACGGGGCTTGCGCAGCTGCGCCGCCTCGACGAGGCCGGCCTGATCATCACCGCCGGCGCGACCGCCGCGGAGGCCGGCGT
CGACCTGGCCGCGCGCACCGCCGCCGACCTCGTCCAGCACGACCACCTCAGCGGCCTCGCCGCGGTGGACAGCCTGGTCG
CCGAGCTGATGACCCGCTCGGGACGCGGACCGGCGCCCGAGGTGCGGCGGGCCCGCGCCCGGCTGGCGGACGGCCTGCTC
GACACCCACTTCGTCCTCGGCGGGGCGCGGATCGCGCTCGCGATGGAGCCCGAGGCGCTGGTCGCCGTCGGCTCCCTGCT
GCACGACGTCGGCGCGGAGATCGTCGCGGCGGTGTCGCCGACGGACGCTCCCGTGCTCGCCACCGCCCCCTGGGACGAGA
TCGTCATCGGCGACCTGACCGACCTGGAGGAACGCGCCCTCGAAGGCGGCGCGGAACTGCTCATCGGGTCGAGTCACGTC
CGCACGGTCGCCGACCGTATCGGCGCCGCCCACCTGGCCGTCGGATTCCCGATCTACGACCGGCTCGGATCGGCCCTGCG
CACGACCGCCGGGTACGGGGGCAGCCTGCGGCTGCTCGTCGACGCGGCGAACCGGCTGCTCGACCACCACCAGGCGGACC
ACCAGGCGAACCACCGGGCCGATCACCGCCCGGGGCGCCACGACGTCCGCGAACATCCGCTCGACTCGTTCGACCAGCTC
GACGTTCTGTGCCAGGAGTCCCCATGTTGA

Upstream 100 bases:

>100_bases
GCGCGGTCGAGCTGGCCCGCCGCCTCGACATGGCGCTGTCGAACCCGGTGTGGGAGCAGGTCCGCGCCCCCGCGCCCTGG
GACGTCGAGGGGGTGGCGTG

Downstream 100 bases:

>100_bases
AGATCGCTTTTGCGACGAGCGACGGCCACGCCGTGGACCAGCACTTCGGCTGGTGCCGCCGGTTCGACGTCTACGAGGTC
GGGCCCGAGGGCTTCCACCT

Product: nitrogenase molybdenum-cofactor biosynthesis protein NifN

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 489; Mature: 488

Protein sequence:

>489_residues
MARVVTSDRRPGLDPLRFSQPLGGALVFLGLAAAMPVMHGSKGCASFAKALLTRHFNEPVPLQTTGVTEVSAVLGSGDDL
VANLDGIRAKQNPRIIGLLTTGVTEVSGEDVAGQVRQYIAMMNHTTPEGAPLIVRVSTPDFAGGLSDGWSAALRSLVATV
PFDHADSDEYPGTRSGFGAGTGSAPETVAVLVGPSLSAADLDELCALIRSFGMAPVLVPDLSGSLDGHLAPSWQPTTTGG
TGLAQLRRLDEAGLIITAGATAAEAGVDLAARTAADLVQHDHLSGLAAVDSLVAELMTRSGRGPAPEVRRARARLADGLL
DTHFVLGGARIALAMEPEALVAVGSLLHDVGAEIVAAVSPTDAPVLATAPWDEIVIGDLTDLEERALEGGAELLIGSSHV
RTVADRIGAAHLAVGFPIYDRLGSALRTTAGYGGSLRLLVDAANRLLDHHQADHQANHRADHRPGRHDVREHPLDSFDQL
DVLCQESPC

Sequences:

>Translated_489_residues
MARVVTSDRRPGLDPLRFSQPLGGALVFLGLAAAMPVMHGSKGCASFAKALLTRHFNEPVPLQTTGVTEVSAVLGSGDDL
VANLDGIRAKQNPRIIGLLTTGVTEVSGEDVAGQVRQYIAMMNHTTPEGAPLIVRVSTPDFAGGLSDGWSAALRSLVATV
PFDHADSDEYPGTRSGFGAGTGSAPETVAVLVGPSLSAADLDELCALIRSFGMAPVLVPDLSGSLDGHLAPSWQPTTTGG
TGLAQLRRLDEAGLIITAGATAAEAGVDLAARTAADLVQHDHLSGLAAVDSLVAELMTRSGRGPAPEVRRARARLADGLL
DTHFVLGGARIALAMEPEALVAVGSLLHDVGAEIVAAVSPTDAPVLATAPWDEIVIGDLTDLEERALEGGAELLIGSSHV
RTVADRIGAAHLAVGFPIYDRLGSALRTTAGYGGSLRLLVDAANRLLDHHQADHQANHRADHRPGRHDVREHPLDSFDQL
DVLCQESPC
>Mature_488_residues
ARVVTSDRRPGLDPLRFSQPLGGALVFLGLAAAMPVMHGSKGCASFAKALLTRHFNEPVPLQTTGVTEVSAVLGSGDDLV
ANLDGIRAKQNPRIIGLLTTGVTEVSGEDVAGQVRQYIAMMNHTTPEGAPLIVRVSTPDFAGGLSDGWSAALRSLVATVP
FDHADSDEYPGTRSGFGAGTGSAPETVAVLVGPSLSAADLDELCALIRSFGMAPVLVPDLSGSLDGHLAPSWQPTTTGGT
GLAQLRRLDEAGLIITAGATAAEAGVDLAARTAADLVQHDHLSGLAAVDSLVAELMTRSGRGPAPEVRRARARLADGLLD
THFVLGGARIALAMEPEALVAVGSLLHDVGAEIVAAVSPTDAPVLATAPWDEIVIGDLTDLEERALEGGAELLIGSSHVR
TVADRIGAAHLAVGFPIYDRLGSALRTTAGYGGSLRLLVDAANRLLDHHQADHQANHRADHRPGRHDVREHPLDSFDQLD
VLCQESPC

Specific function: This protein may play a role in the biosynthesis of the prosthetic group of nitrogenase (FeMo cofactor) [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR000318
- InterPro:   IPR005975 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: NA

Molecular weight: Translated: 50740; Mature: 50609

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARVVTSDRRPGLDPLRFSQPLGGALVFLGLAAAMPVMHGSKGCASFAKALLTRHFNEPV
CCCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCC
PLQTTGVTEVSAVLGSGDDLVANLDGIRAKQNPRIIGLLTTGVTEVSGEDVAGQVRQYIA
CCEECCCHHHHHHHCCCCCEEECCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHH
MMNHTTPEGAPLIVRVSTPDFAGGLSDGWSAALRSLVATVPFDHADSDEYPGTRSGFGAG
HHHCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
TGSAPETVAVLVGPSLSAADLDELCALIRSFGMAPVLVPDLSGSLDGHLAPSWQPTTTGG
CCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCCCC
TGLAQLRRLDEAGLIITAGATAAEAGVDLAARTAADLVQHDHLSGLAAVDSLVAELMTRS
HHHHHHHHHCCCCEEEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GRGPAPEVRRARARLADGLLDTHFVLGGARIALAMEPEALVAVGSLLHDVGAEIVAAVSP
CCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHHHHEEEECC
TDAPVLATAPWDEIVIGDLTDLEERALEGGAELLIGSSHVRTVADRIGAAHLAVGFPIYD
CCCCEEEECCCCCEEECCHHHHHHHHHCCCCEEEECCHHHHHHHHHHCHHHHHCCCHHHH
RLGSALRTTAGYGGSLRLLVDAANRLLDHHQADHQANHRADHRPGRHDVREHPLDSFDQL
HHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCHHHH
DVLCQESPC
HHHHCCCCC
>Mature Secondary Structure 
ARVVTSDRRPGLDPLRFSQPLGGALVFLGLAAAMPVMHGSKGCASFAKALLTRHFNEPV
CCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCC
PLQTTGVTEVSAVLGSGDDLVANLDGIRAKQNPRIIGLLTTGVTEVSGEDVAGQVRQYIA
CCEECCCHHHHHHHCCCCCEEECCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHH
MMNHTTPEGAPLIVRVSTPDFAGGLSDGWSAALRSLVATVPFDHADSDEYPGTRSGFGAG
HHHCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
TGSAPETVAVLVGPSLSAADLDELCALIRSFGMAPVLVPDLSGSLDGHLAPSWQPTTTGG
CCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCCCC
TGLAQLRRLDEAGLIITAGATAAEAGVDLAARTAADLVQHDHLSGLAAVDSLVAELMTRS
HHHHHHHHHCCCCEEEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GRGPAPEVRRARARLADGLLDTHFVLGGARIALAMEPEALVAVGSLLHDVGAEIVAAVSP
CCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHHHHEEEECC
TDAPVLATAPWDEIVIGDLTDLEERALEGGAELLIGSSHVRTVADRIGAAHLAVGFPIYD
CCCCEEEECCCCCEEECCHHHHHHHHHCCCCEEEECCHHHHHHHHHHCHHHHHCCCHHHH
RLGSALRTTAGYGGSLRLLVDAANRLLDHHQADHQANHRADHRPGRHDVREHPLDSFDQL
HHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCHHHH
DVLCQESPC
HHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2266945; 11157954; 12597275 [H]