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The map label for this gene is cocE [H]

Identifier: 158318554

GI number: 158318554

Start: 8312215

End: 8313726

Strand: Direct

Name: cocE [H]

Synonym: Franean1_6822

Alternate gene names: 158318554

Gene position: 8312215-8313726 (Clockwise)

Preceding gene: 158318553

Following gene: 158318558

Centisome position: 92.54

GC content: 72.75

Gene sequence:

>1512_bases
GTGGGCGCGGTGGCGGCGCTGACCGCCTGCTCGGCGTCCACCACCGGTGACGGCAGGCCCACCGGGGAGACCGAGGCCTC
CGCCGGCGGCCTCCGCCCGCAGACGGCCCCGCCGACCGCGACCGGCCCGCTCTCGGCCGCGACGCAGGCCTGGTGGGCGT
ACACCCGGCAGCCGACCTTCGAGTCGGTCCGCACCACGGTGCCGGTGCCGGTGCCGGTGCCGGTGCCGGTGCCGGACGGC
ACCCTCCTCGGCTGCTCGCTCGTGCGGCCGGCGAAGGACGGCACCGCCGCCCCCGGGCGCTTCCCCGGCCTGGTGATCGA
GTTCACCCCGTACGCGGTGATCCGCTCGACGTTCGAGAGCGAGGCCGCCTACTTCGCCACCCGTGGCTACAACGGTCTGG
TGTGCAACGTCCGGGGCACCGGGCCGTCCGGGGGCACCTGGCAGAACGCCATGTCGGCGCAGGACGGCCGGGACGCCCGT
GACCTCGTCGAATGGCTGGCGACCCAGCCGTTCTCGAACGGGCGCATCGGCATGTTCGGGGAGAGCTACGGCGGTCAGAC
CACCTACGGCGCCGCCGTCGAGCAGGCGCCGCACCTGCTGGCCGCCGCGCCCCTGCAGTCCCCGGCCAACCTCTATGACG
ACGTCATCTACCCGGGCGGCATCAAGGCGACCGAGCGCGGGAAGATCGACAACTGGCCGCCCACCGGGCAGTTGCCCAGC
CAGGACCGCATCAACGCCGACGCCGAGTTCGCCACCAACCGCCAGCATCCGACCTTCGATTCCTACTGGCAGGAACGCGC
GCTGCCCGACCGGGTCGACGCGATCAAGGTTCCCGTTCTCGCTCTCGGCGGGTACCAGGACGGCTACTTCCGCGCCGGCA
CGCTCGCCAACATCGAGGGCGCCCTCGACCGGACGTGGGCGGTCTACGGCCCCTGGCCGCACCTGTCACCCATCGCGTTT
CCGGACTGCCCTGGCCTGTGTAATCCGAAAGGGCTCGCGCCGGGCCTGCTGCTCGCCTGGTTCGACCGCTGGGTGATGGA
TCTGCCGGACACCCCCGTGCCGGACCACCCGACGTTCGTGAGTTACGAGGGCCCCGAAGGCACCGGCGCCGGCTGGCGTG
AGGTCTCGAACTACGACCCGGCCGCCCGCGGCCGCACCGGGACACTGCTCCTCGGCAAGGACGGCGTCCTCACCGAGGCG
GGCGCCAACTTCTACGTGGAGCTGCTCGACGTAGCCCCGGACGGCGCGTCCACGGTGGTGAACGATGGTTTCCTGCGGGC
CAGCCACCGCGAGTCGCACACGTCACCGACGCCGGTGACCGCCGGGGAGCCCACCGCCTTCGAGATCGGCATCCGGCCGG
TCCACCACCGCTTCGCCGCCGGCCACCGCATCGGTGTCCGGGTCTCCGGCGGCGCCGCCGACGTCCTCACTCCCAACGCG
AGCCCGGTCGACGTGACCGTCAGCACCGGCCCTGGAACCTCCACCCTGGTCATCCCTACCGTGCCCGAGTAG

Upstream 100 bases:

>100_bases
GCCGGCGGCCAGTTAGCCGGCGTTTTATCCGGTCGGAGGTGCCCCTCGGGGCACCTCCGGGGGCGGGCTGACGGCCGCCA
GGTTCACCGCGCTGGTCACC

Downstream 100 bases:

>100_bases
GACGGTCTCCGCGGCGGTGGCCGGCACCGCCGCGGGTGGGCGCATGGTGGGGGCGGCGTCGGCGTCCCGAACGCGCAGCG
CGGCGAGGAGCCCGAGGGCG

Product: peptidase S15

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 503; Mature: 502

Protein sequence:

>503_residues
MGAVAALTACSASTTGDGRPTGETEASAGGLRPQTAPPTATGPLSAATQAWWAYTRQPTFESVRTTVPVPVPVPVPVPDG
TLLGCSLVRPAKDGTAAPGRFPGLVIEFTPYAVIRSTFESEAAYFATRGYNGLVCNVRGTGPSGGTWQNAMSAQDGRDAR
DLVEWLATQPFSNGRIGMFGESYGGQTTYGAAVEQAPHLLAAAPLQSPANLYDDVIYPGGIKATERGKIDNWPPTGQLPS
QDRINADAEFATNRQHPTFDSYWQERALPDRVDAIKVPVLALGGYQDGYFRAGTLANIEGALDRTWAVYGPWPHLSPIAF
PDCPGLCNPKGLAPGLLLAWFDRWVMDLPDTPVPDHPTFVSYEGPEGTGAGWREVSNYDPAARGRTGTLLLGKDGVLTEA
GANFYVELLDVAPDGASTVVNDGFLRASHRESHTSPTPVTAGEPTAFEIGIRPVHHRFAAGHRIGVRVSGGAADVLTPNA
SPVDVTVSTGPGTSTLVIPTVPE

Sequences:

>Translated_503_residues
MGAVAALTACSASTTGDGRPTGETEASAGGLRPQTAPPTATGPLSAATQAWWAYTRQPTFESVRTTVPVPVPVPVPVPDG
TLLGCSLVRPAKDGTAAPGRFPGLVIEFTPYAVIRSTFESEAAYFATRGYNGLVCNVRGTGPSGGTWQNAMSAQDGRDAR
DLVEWLATQPFSNGRIGMFGESYGGQTTYGAAVEQAPHLLAAAPLQSPANLYDDVIYPGGIKATERGKIDNWPPTGQLPS
QDRINADAEFATNRQHPTFDSYWQERALPDRVDAIKVPVLALGGYQDGYFRAGTLANIEGALDRTWAVYGPWPHLSPIAF
PDCPGLCNPKGLAPGLLLAWFDRWVMDLPDTPVPDHPTFVSYEGPEGTGAGWREVSNYDPAARGRTGTLLLGKDGVLTEA
GANFYVELLDVAPDGASTVVNDGFLRASHRESHTSPTPVTAGEPTAFEIGIRPVHHRFAAGHRIGVRVSGGAADVLTPNA
SPVDVTVSTGPGTSTLVIPTVPE
>Mature_502_residues
GAVAALTACSASTTGDGRPTGETEASAGGLRPQTAPPTATGPLSAATQAWWAYTRQPTFESVRTTVPVPVPVPVPVPDGT
LLGCSLVRPAKDGTAAPGRFPGLVIEFTPYAVIRSTFESEAAYFATRGYNGLVCNVRGTGPSGGTWQNAMSAQDGRDARD
LVEWLATQPFSNGRIGMFGESYGGQTTYGAAVEQAPHLLAAAPLQSPANLYDDVIYPGGIKATERGKIDNWPPTGQLPSQ
DRINADAEFATNRQHPTFDSYWQERALPDRVDAIKVPVLALGGYQDGYFRAGTLANIEGALDRTWAVYGPWPHLSPIAFP
DCPGLCNPKGLAPGLLLAWFDRWVMDLPDTPVPDHPTFVSYEGPEGTGAGWREVSNYDPAARGRTGTLLLGKDGVLTEAG
ANFYVELLDVAPDGASTVVNDGFLRASHRESHTSPTPVTAGEPTAFEIGIRPVHHRFAAGHRIGVRVSGGAADVLTPNAS
PVDVTVSTGPGTSTLVIPTVPE

Specific function: Hydrolyzes cocaine to benzoate and ecgonine methyl ester, endowing the bacteria with the ability to utilize cocaine as a sole source of carbon and energy for growth, as this bacterium lives in the rhizosphere of coca plants [H]

COG id: COG2936

COG function: function code R; Predicted acyl esterases

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha/beta hydrolase family. Carboxylesterase subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005674
- InterPro:   IPR008979
- InterPro:   IPR013736
- InterPro:   IPR000383 [H]

Pfam domain/function: PF02129 Peptidase_S15; PF08530 PepX_C [H]

EC number: NA

Molecular weight: Translated: 52952; Mature: 52821

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00131 CARBOXYPEPT_SER_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGAVAALTACSASTTGDGRPTGETEASAGGLRPQTAPPTATGPLSAATQAWWAYTRQPTF
CCHHHEEHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCH
ESVRTTVPVPVPVPVPVPDGTLLGCSLVRPAKDGTAAPGRFPGLVIEFTPYAVIRSTFES
HHHHEECCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEECCHHHHHHHHHH
EAAYFATRGYNGLVCNVRGTGPSGGTWQNAMSAQDGRDARDLVEWLATQPFSNGRIGMFG
CCEEEEECCCCEEEEEEECCCCCCCCHHHHCCCCCCCCHHHHHHHHHHCCCCCCEEEECC
ESYGGQTTYGAAVEQAPHLLAAAPLQSPANLYDDVIYPGGIKATERGKIDNWPPTGQLPS
CCCCCCCCCCHHHHHCCCEEEECCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
QDRINADAEFATNRQHPTFDSYWQERALPDRVDAIKVPVLALGGYQDGYFRAGTLANIEG
CCCCCCCHHHHCCCCCCCHHHHHHHHCCCCHHCEEEEEEEEECCCCCCCEECCCCCCCCC
ALDRTWAVYGPWPHLSPIAFPDCPGLCNPKGLAPGLLLAWFDRWVMDLPDTPVPDHPTFV
CCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEE
SYEGPEGTGAGWREVSNYDPAARGRTGTLLLGKDGVLTEAGANFYVELLDVAPDGASTVV
EECCCCCCCCCHHHHCCCCCCCCCCCEEEEECCCCCEEECCCCEEEEEEEECCCCCCHHH
NDGFLRASHRESHTSPTPVTAGEPTAFEIGIRPVHHRFAAGHRIGVRVSGGAADVLTPNA
CCCEEECCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHCCCEEEEEECCCCCEEECCCC
SPVDVTVSTGPGTSTLVIPTVPE
CCEEEEEECCCCCCEEEEECCCC
>Mature Secondary Structure 
GAVAALTACSASTTGDGRPTGETEASAGGLRPQTAPPTATGPLSAATQAWWAYTRQPTF
CHHHEEHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCH
ESVRTTVPVPVPVPVPVPDGTLLGCSLVRPAKDGTAAPGRFPGLVIEFTPYAVIRSTFES
HHHHEECCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEECCHHHHHHHHHH
EAAYFATRGYNGLVCNVRGTGPSGGTWQNAMSAQDGRDARDLVEWLATQPFSNGRIGMFG
CCEEEEECCCCEEEEEEECCCCCCCCHHHHCCCCCCCCHHHHHHHHHHCCCCCCEEEECC
ESYGGQTTYGAAVEQAPHLLAAAPLQSPANLYDDVIYPGGIKATERGKIDNWPPTGQLPS
CCCCCCCCCCHHHHHCCCEEEECCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
QDRINADAEFATNRQHPTFDSYWQERALPDRVDAIKVPVLALGGYQDGYFRAGTLANIEG
CCCCCCCHHHHCCCCCCCHHHHHHHHCCCCHHCEEEEEEEEECCCCCCCEECCCCCCCCC
ALDRTWAVYGPWPHLSPIAFPDCPGLCNPKGLAPGLLLAWFDRWVMDLPDTPVPDHPTFV
CCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEE
SYEGPEGTGAGWREVSNYDPAARGRTGTLLLGKDGVLTEAGANFYVELLDVAPDGASTVV
EECCCCCCCCCHHHHCCCCCCCCCCCEEEEECCCCCEEECCCCEEEEEEEECCCCCCHHH
NDGFLRASHRESHTSPTPVTAGEPTAFEIGIRPVHHRFAAGHRIGVRVSGGAADVLTPNA
CCCEEECCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHCCCEEEEEECCCCCEEECCCC
SPVDVTVSTGPGTSTLVIPTVPE
CCEEEEEECCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10698749; 11742345; 12369817 [H]