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The map label for this gene is livK [C]

Identifier: 158318209

GI number: 158318209

Start: 7870130

End: 7871302

Strand: Reverse

Name: livK [C]

Synonym: Franean1_6474

Alternate gene names: 158318209

Gene position: 7871302-7870130 (Counterclockwise)

Preceding gene: 158318210

Following gene: 158318208

Centisome position: 87.63

GC content: 70.25

Gene sequence:

>1173_bases
ATGATCAGGCGACCGGCCCTGGGCGCGATCGTCGCCGCCGCCATGACGGTGATGATCGTCGCGGCCTGCGGCGGTGATGG
TGTCTCCTCGTCCGGCGACCGCCGCCGGACGGTCACCATCGGGTTCCAGGGCGTGCTGTCGGGCGACGACGAGCAGCTGG
GTGTCAACGCCCTCTACGGGGTGCGCACGGCGATCGAGGAGATCAACGCGGACGCCAGCCTGCCGTTCCGGGTCGAGCTC
GTCTCCTCCGACGACGGCGGGTCACCCGAGCGGGCGCCGGCCGCCGCGCGCAGGCTCATCGACGACTCCGATGCGGTGGC
CGTCATCGGCCCGATGCTCTCGGACGCGACAGAGGCCAGCGAGCCGTTCTACAGCGAGGCCGGCCTGCTCTCGGTCAGCC
CGTCCGCCACCGCCCCGGCGCTCACCGACCTCGGCTTCACCACCTTCTACCGGGTCGTCGCCCCCGACTCCGTGCAGGGC
CGGGCCGCGGCCGACTACATCTCCACCGTCCTCGACGCGGGGAAGGTGTACTCGGTCGACGACCGCAGCGAGTACGGCAT
CGGCCTGTCCACCGCGCTGGAGGCGGAGCTGTTCTCCGACGGCGTCGACGTCGTCCACGGCGGCGTCGACCCGGCCCGGG
ACTACACCCGCGAGGCAGCGGAGATCATCGCTGAGGCGCCGGACGTACTCTTCTACTCCGGTCAGTACGCCGACTTCGCG
CTGCTCAACAAGGCGCTCGAGGACGGTGGCTTCACGGGCACCGTCATGGGCGGGGACGCGACCCGTGACCCGGACTTCGT
CACGCGGACGGGCGCGGCTGCCGCCGAGGGCGCCTACTTCTCGTGCGCCTGCAGCGACGTCGACACCGACCCGGCGGCGA
AGGAGTTCGTGGCCTCGTACAAGGACGTCAACGGCGGGGCGTTGCCCGGGACCTACTCGGGGGAGGCCTACGACGCCACC
TGGGCCGTCGTCGACGTCCTGAAGCGGCTCGGGAACAACATCAGCCGGGAGTCCGTCGCGCGGGCCTTCCGCACCGTCAG
CTGGGACGGCCTCACCAGGAAGATCGAGTTCGAGGAGAACGGCGAGCTCAAGCGCTCGACCGTCTACCTGTACAAGGTGC
AGGACGGTGAGATCACGGATCTTGGACGGATCTCGGACCTGATCGGCTCCTGA

Upstream 100 bases:

>100_bases
CGTGGACTCCGGGCCGCGGCCGGACCTCCCCCTCGGCTCGCCGTCGAGAATCAGGTGCCGGACTGCCCGCGGGCGGCGGT
GAAACCGGAGGAGAGACAGC

Downstream 100 bases:

>100_bases
CACTCGCCCGTCGCCCGTCAGCGGTGCTCGTGTTTCGGTGCGTGGCCTCGTATGGCCTAGTCGCTGTTGGCGAGGGACGA
TGTGCTTTCCGTGTCGCGTC

Product: extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MIRRPALGAIVAAAMTVMIVAACGGDGVSSSGDRRRTVTIGFQGVLSGDDEQLGVNALYGVRTAIEEINADASLPFRVEL
VSSDDGGSPERAPAAARRLIDDSDAVAVIGPMLSDATEASEPFYSEAGLLSVSPSATAPALTDLGFTTFYRVVAPDSVQG
RAAADYISTVLDAGKVYSVDDRSEYGIGLSTALEAELFSDGVDVVHGGVDPARDYTREAAEIIAEAPDVLFYSGQYADFA
LLNKALEDGGFTGTVMGGDATRDPDFVTRTGAAAAEGAYFSCACSDVDTDPAAKEFVASYKDVNGGALPGTYSGEAYDAT
WAVVDVLKRLGNNISRESVARAFRTVSWDGLTRKIEFEENGELKRSTVYLYKVQDGEITDLGRISDLIGS

Sequences:

>Translated_390_residues
MIRRPALGAIVAAAMTVMIVAACGGDGVSSSGDRRRTVTIGFQGVLSGDDEQLGVNALYGVRTAIEEINADASLPFRVEL
VSSDDGGSPERAPAAARRLIDDSDAVAVIGPMLSDATEASEPFYSEAGLLSVSPSATAPALTDLGFTTFYRVVAPDSVQG
RAAADYISTVLDAGKVYSVDDRSEYGIGLSTALEAELFSDGVDVVHGGVDPARDYTREAAEIIAEAPDVLFYSGQYADFA
LLNKALEDGGFTGTVMGGDATRDPDFVTRTGAAAAEGAYFSCACSDVDTDPAAKEFVASYKDVNGGALPGTYSGEAYDAT
WAVVDVLKRLGNNISRESVARAFRTVSWDGLTRKIEFEENGELKRSTVYLYKVQDGEITDLGRISDLIGS
>Mature_390_residues
MIRRPALGAIVAAAMTVMIVAACGGDGVSSSGDRRRTVTIGFQGVLSGDDEQLGVNALYGVRTAIEEINADASLPFRVEL
VSSDDGGSPERAPAAARRLIDDSDAVAVIGPMLSDATEASEPFYSEAGLLSVSPSATAPALTDLGFTTFYRVVAPDSVQG
RAAADYISTVLDAGKVYSVDDRSEYGIGLSTALEAELFSDGVDVVHGGVDPARDYTREAAEIIAEAPDVLFYSGQYADFA
LLNKALEDGGFTGTVMGGDATRDPDFVTRTGAAAAEGAYFSCACSDVDTDPAAKEFVASYKDVNGGALPGTYSGEAYDAT
WAVVDVLKRLGNNISRESVARAFRTVSWDGLTRKIEFEENGELKRSTVYLYKVQDGEITDLGRISDLIGS

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=330, Percent_Identity=26.0606060606061, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI48994941, Length=334, Percent_Identity=25.748502994012, Blast_Score=101, Evalue=6e-23,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 40926; Mature: 40926

Theoretical pI: Translated: 4.06; Mature: 4.06

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRRPALGAIVAAAMTVMIVAACGGDGVSSSGDRRRTVTIGFQGVLSGDDEQLGVNALYG
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEEEECCCCCCCCHHHHHH
VRTAIEEINADASLPFRVELVSSDDGGSPERAPAAARRLIDDSDAVAVIGPMLSDATEAS
HHHHHHHHCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHCCCCCCEEEECHHHHCHHHCC
EPFYSEAGLLSVSPSATAPALTDLGFTTFYRVVAPDSVQGRAAADYISTVLDAGKVYSVD
CCCHHCCCEEEECCCCCCCHHHHCCHHHEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEC
DRSEYGIGLSTALEAELFSDGVDVVHGGVDPARDYTREAAEIIAEAPDVLFYSGQYADFA
CCCCCCCCHHHHHHHHHHHCCHHEEECCCCHHHHHHHHHHHHHHCCCCEEEECCCCHHHH
LLNKALEDGGFTGTVMGGDATRDPDFVTRTGAAAAEGAYFSCACSDVDTDPAAKEFVASY
HHHHHHHCCCCEEEEECCCCCCCCCHHEECCCCHHCCCEEEEECCCCCCCHHHHHHHHHH
KDVNGGALPGTYSGEAYDATWAVVDVLKRLGNNISRESVARAFRTVSWDGLTRKIEFEEN
HCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCEEEEEECCC
GELKRSTVYLYKVQDGEITDLGRISDLIGS
CCEEEEEEEEEEECCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MIRRPALGAIVAAAMTVMIVAACGGDGVSSSGDRRRTVTIGFQGVLSGDDEQLGVNALYG
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEEEECCCCCCCCHHHHHH
VRTAIEEINADASLPFRVELVSSDDGGSPERAPAAARRLIDDSDAVAVIGPMLSDATEAS
HHHHHHHHCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHCCCCCCEEEECHHHHCHHHCC
EPFYSEAGLLSVSPSATAPALTDLGFTTFYRVVAPDSVQGRAAADYISTVLDAGKVYSVD
CCCHHCCCEEEECCCCCCCHHHHCCHHHEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEC
DRSEYGIGLSTALEAELFSDGVDVVHGGVDPARDYTREAAEIIAEAPDVLFYSGQYADFA
CCCCCCCCHHHHHHHHHHHCCHHEEECCCCHHHHHHHHHHHHHHCCCCEEEECCCCHHHH
LLNKALEDGGFTGTVMGGDATRDPDFVTRTGAAAAEGAYFSCACSDVDTDPAAKEFVASY
HHHHHHHCCCCEEEEECCCCCCCCCHHEECCCCHHCCCEEEEECCCCCCCHHHHHHHHHH
KDVNGGALPGTYSGEAYDATWAVVDVLKRLGNNISRESVARAFRTVSWDGLTRKIEFEEN
HCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCEEEEEECCC
GELKRSTVYLYKVQDGEITDLGRISDLIGS
CCEEEEEEEEEEECCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]