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The map label for this gene is metB [H]

Identifier: 158318160

GI number: 158318160

Start: 7805191

End: 7806348

Strand: Direct

Name: metB [H]

Synonym: Franean1_6425

Alternate gene names: 158318160

Gene position: 7805191-7806348 (Clockwise)

Preceding gene: 158318159

Following gene: 158318161

Centisome position: 86.9

GC content: 75.22

Gene sequence:

>1158_bases
ATGGGTGATCGGAACTCGGGATTCGAGACCGTCGCGGTGCACGCGGGCCAGGAGCCCGACCCTGCCACCGGCGCGGTCGT
CGTCCCCATCCATCCCGCGTCGACGTTCGCGCAGGACGGCGTCGGCGGGCTGCGGGCCGGCTTCGAGTACGCCCGCTCGG
GCAACCCGACCCGCGCCGCGCTGGAGGCCAGCCTGACCGCGCTCGAGCGCGGGGCCGCGGCGCTCGCGTTCGCCTCCGGT
CTGGCCGCCTCGGACGCGCTGCTGCGGGCCGTGTGCCGTCCCGGCAGCCACGTGGTGATCCCCGGCGACGCCTACGGCGG
CACGTACCGCCTGTTCGCGCGGGTGCTAGCCGAGTGGGGCGTCGAGCACACGGCGGTCGACCTGAGCGACCCGGCCGCCG
CCGTGGCCGCGATCCGGCCGGGGGCGACGGCGCTGGTGTGGTGCGAGACGCCGACCAACCCGATGCTCGCGATCGCCGAC
ATCGCCGCGCTCGCCGCCGTCGCGCACCAGGCGGGCGCGCTGCTCGTCGTCGACAACACCTTCGCCTCGCCCTACCTGTG
CCAGCCGCTGACGCTCGGCGCGGACGCGGTCGTCCACTCGACCACGAAGTACCTCGGCGGGCACAGCGACGTGGTGGGCG
GGGCGGTGGTGACCGCGTCGGCGGAGCTCGCCGAGCGGGTGCGGTTCGTACAGAACGCTGCCGGGGCCGTGCCGGGGCCG
TTCGACGCCTGGCTGGTGCTGCGCGGGATCAGGACCCTCGCGGTGCGGATGGACCGGCACTGCGCGAACGCGCGGGAGAT
CGCCGCGCTGCTCGCCGGCCACGCCGCCGTGTCGGCCGTCCACTACCCGGGGCTGGCGAGCCACCCCGGCCACGACGTCG
CTGCCAAGCAGATGCGTGACTTCGGCGGGATGGTCTCGTTCCAGGTCCAGGCCGGCGCGGAGGCCGCCCTCGCGGTGTGC
GCCGCCACCCGGCTGTTCACCCTCGCCGAGTCGCTGGGCGGGGTGGAGTCGCTGATCGAGCATCCCGCGCGGATGACGCA
CGCTTCGGTGGCGGGCTCACCGCTCGCCGTCCCCGACGACCTCGTGCGGCTCTCCGTCGGCATCGAGGCGGCCGACGACC
TGGTCGCCGACCTCGAACAGGCACTCGACTCGGCCTGA

Upstream 100 bases:

>100_bases
CCGCTGAGATCCGGTGAGATCCCGCTCCGCCTCCTGAAATCGCCGCTTGTTGAAGTCGCCGCTTGTTTGAAGTCCGGGCT
CGAGTGCGAGGGTTGTGGCC

Downstream 100 bases:

>100_bases
CGCGCGGTTTCCCGCAAACCGCCGCTTGCCGACCCGACCCGGCCCGGCGGTTACCCGCCGGGCCGGGCTCATGAGGTGTG
GGAGAAGCCAAGCACACTCC

Product: cystathionine gamma-synthase

Products: NA

Alternate protein names: CGS; O-succinylhomoserine (thiol)-lyase [H]

Number of amino acids: Translated: 385; Mature: 384

Protein sequence:

>385_residues
MGDRNSGFETVAVHAGQEPDPATGAVVVPIHPASTFAQDGVGGLRAGFEYARSGNPTRAALEASLTALERGAAALAFASG
LAASDALLRAVCRPGSHVVIPGDAYGGTYRLFARVLAEWGVEHTAVDLSDPAAAVAAIRPGATALVWCETPTNPMLAIAD
IAALAAVAHQAGALLVVDNTFASPYLCQPLTLGADAVVHSTTKYLGGHSDVVGGAVVTASAELAERVRFVQNAAGAVPGP
FDAWLVLRGIRTLAVRMDRHCANAREIAALLAGHAAVSAVHYPGLASHPGHDVAAKQMRDFGGMVSFQVQAGAEAALAVC
AATRLFTLAESLGGVESLIEHPARMTHASVAGSPLAVPDDLVRLSVGIEAADDLVADLEQALDSA

Sequences:

>Translated_385_residues
MGDRNSGFETVAVHAGQEPDPATGAVVVPIHPASTFAQDGVGGLRAGFEYARSGNPTRAALEASLTALERGAAALAFASG
LAASDALLRAVCRPGSHVVIPGDAYGGTYRLFARVLAEWGVEHTAVDLSDPAAAVAAIRPGATALVWCETPTNPMLAIAD
IAALAAVAHQAGALLVVDNTFASPYLCQPLTLGADAVVHSTTKYLGGHSDVVGGAVVTASAELAERVRFVQNAAGAVPGP
FDAWLVLRGIRTLAVRMDRHCANAREIAALLAGHAAVSAVHYPGLASHPGHDVAAKQMRDFGGMVSFQVQAGAEAALAVC
AATRLFTLAESLGGVESLIEHPARMTHASVAGSPLAVPDDLVRLSVGIEAADDLVADLEQALDSA
>Mature_384_residues
GDRNSGFETVAVHAGQEPDPATGAVVVPIHPASTFAQDGVGGLRAGFEYARSGNPTRAALEASLTALERGAAALAFASGL
AASDALLRAVCRPGSHVVIPGDAYGGTYRLFARVLAEWGVEHTAVDLSDPAAAVAAIRPGATALVWCETPTNPMLAIADI
AALAAVAHQAGALLVVDNTFASPYLCQPLTLGADAVVHSTTKYLGGHSDVVGGAVVTASAELAERVRFVQNAAGAVPGPF
DAWLVLRGIRTLAVRMDRHCANAREIAALLAGHAAVSAVHYPGLASHPGHDVAAKQMRDFGGMVSFQVQAGAEAALAVCA
ATRLFTLAESLGGVESLIEHPARMTHASVAGSPLAVPDDLVRLSVGIEAADDLVADLEQALDSA

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=383, Percent_Identity=47.5195822454308, Blast_Score=315, Evalue=3e-86,
Organism=Homo sapiens, GI299473759, Length=383, Percent_Identity=44.3864229765013, Blast_Score=277, Evalue=1e-74,
Organism=Homo sapiens, GI25453487, Length=382, Percent_Identity=41.6230366492147, Blast_Score=248, Evalue=5e-66,
Organism=Escherichia coli, GI1790375, Length=381, Percent_Identity=41.994750656168, Blast_Score=279, Evalue=3e-76,
Organism=Escherichia coli, GI1789383, Length=393, Percent_Identity=30.2798982188295, Blast_Score=151, Evalue=6e-38,
Organism=Caenorhabditis elegans, GI17538055, Length=385, Percent_Identity=47.5324675324675, Blast_Score=322, Evalue=2e-88,
Organism=Caenorhabditis elegans, GI17560056, Length=385, Percent_Identity=46.7532467532467, Blast_Score=318, Evalue=3e-87,
Organism=Caenorhabditis elegans, GI71980708, Length=372, Percent_Identity=40.3225806451613, Blast_Score=265, Evalue=3e-71,
Organism=Saccharomyces cerevisiae, GI6319307, Length=388, Percent_Identity=48.7113402061856, Blast_Score=341, Evalue=1e-94,
Organism=Saccharomyces cerevisiae, GI6321254, Length=396, Percent_Identity=32.0707070707071, Blast_Score=183, Evalue=5e-47,
Organism=Saccharomyces cerevisiae, GI6323333, Length=441, Percent_Identity=31.7460317460317, Blast_Score=178, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6321170, Length=323, Percent_Identity=31.5789473684211, Blast_Score=145, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6321904, Length=390, Percent_Identity=29.2307692307692, Blast_Score=142, Evalue=9e-35,
Organism=Drosophila melanogaster, GI19922554, Length=386, Percent_Identity=45.5958549222798, Blast_Score=335, Evalue=5e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =2.5.1.48 [H]

Molecular weight: Translated: 39171; Mature: 39039

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGDRNSGFETVAVHAGQEPDPATGAVVVPIHPASTFAQDGVGGLRAGFEYARSGNPTRAA
CCCCCCCCEEEEEECCCCCCCCCCEEEEEECCCHHHHHCCCCHHHHHHHHHHCCCCHHHH
LEASLTALERGAAALAFASGLAASDALLRAVCRPGSHVVIPGDAYGGTYRLFARVLAEWG
HHHHHHHHHHCHHHHHHHHCCHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHC
VEHTAVDLSDPAAAVAAIRPGATALVWCETPTNPMLAIADIAALAAVAHQAGALLVVDNT
CCEEEEECCCCHHHHHEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCEEEEEECC
FASPYLCQPLTLGADAVVHSTTKYLGGHSDVVGGAVVTASAELAERVRFVQNAAGAVPGP
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHEECCHHHHHHHHHHHHHCCCCCCC
FDAWLVLRGIRTLAVRMDRHCANAREIAALLAGHAAVSAVHYPGLASHPGHDVAAKQMRD
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
FGGMVSFQVQAGAEAALAVCAATRLFTLAESLGGVESLIEHPARMTHASVAGSPLAVPDD
HCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHCHHHHHHHHCCCCCCCCCHH
LVRLSVGIEAADDLVADLEQALDSA
HHEEEECCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GDRNSGFETVAVHAGQEPDPATGAVVVPIHPASTFAQDGVGGLRAGFEYARSGNPTRAA
CCCCCCCEEEEEECCCCCCCCCCEEEEEECCCHHHHHCCCCHHHHHHHHHHCCCCHHHH
LEASLTALERGAAALAFASGLAASDALLRAVCRPGSHVVIPGDAYGGTYRLFARVLAEWG
HHHHHHHHHHCHHHHHHHHCCHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHC
VEHTAVDLSDPAAAVAAIRPGATALVWCETPTNPMLAIADIAALAAVAHQAGALLVVDNT
CCEEEEECCCCHHHHHEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCEEEEEECC
FASPYLCQPLTLGADAVVHSTTKYLGGHSDVVGGAVVTASAELAERVRFVQNAAGAVPGP
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHEECCHHHHHHHHHHHHHCCCCCCC
FDAWLVLRGIRTLAVRMDRHCANAREIAALLAGHAAVSAVHYPGLASHPGHDVAAKQMRD
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
FGGMVSFQVQAGAEAALAVCAATRLFTLAESLGGVESLIEHPARMTHASVAGSPLAVPDD
HCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHCHHHHHHHHCCCCCCCCCHH
LVRLSVGIEAADDLVADLEQALDSA
HHEEEECCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]