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The map label for this gene is liaR [H]

Identifier: 158318087

GI number: 158318087

Start: 7710816

End: 7711550

Strand: Reverse

Name: liaR [H]

Synonym: Franean1_6351

Alternate gene names: 158318087

Gene position: 7711550-7710816 (Counterclockwise)

Preceding gene: 158318088

Following gene: 158318086

Centisome position: 85.86

GC content: 65.44

Gene sequence:

>735_bases
GTGACAGCGGACGGCCGCTGGGAAGATCCCGGTTGTGTCCGCGACACCCCGAACATCAAACGCGCCCAGGCTGGCCCGGT
GCCGGTTTCAGTCCTCCTTGTCGACGACGAGGAACTGGTGCGGGTGGGGCTGCGGGTCATCCTGGAGTCAGGTGGCGAGA
TCGACGTTGTTGGAGAGGCCTCCGACGGCTCACAGGTCGTGCCACTGATCCGGGAGAATACCCCGGACGTCGTAATCATG
GACATTCGGATGCCGAGGGTCGACGGGCTGGCGGCCATCCGGGAGATCCTCGCAGACGTTGCCGACCCCCCGAAGATCCT
CGTGCTCACCACCTTCGAGCTGGACGACTATGTTTACGAAGCGCTCCGCAGCGGGGCCAACGGGTTCCTGCTCAAACGAG
CCCGGCCCGCGGAGATCGTCCAGGCCGTCCGCACGATTGTCTCCAGTGAGAGCCTGCTGTTTCCCGAAGCGATCCGGTAC
ATGGCAGGCAAGCGACCGAACCGGGAGGCCCACGAGACGATGCGACGGGCCGTGCTCACTGACCGGGAGGCCGACGTACT
CCGGCTGATCAGCGAGGGACTGTCCAACAACGAGATCGCCAACCGGCTGTACCTCGGTGCCCAGACCGTCAAGAGCCACG
TGAGCAGCGTGCTGGCCAAGCTCGGCGCACGTGACCGTACCCAGGCGGTCATCATCGCCTACGAGTCCGGCTTCGTGGAG
GCCGGCAAGCCCTAG

Upstream 100 bases:

>100_bases
CCCGGCTTCCCCTCGGCATGGGATTCGGTGTTCAGGCTGTGCGTCGCGACCTGGTCGGTTCCAGTTACGACAATGGCACC
GCCACAGCGGACGGTCGGTC

Downstream 100 bases:

>100_bases
CGCGGCCGCCGCCAGCTACGGACGGTGATAGCGAAGAGAAGGGCGGTCCGATTGAGAAGACATCGTCTCCAACTGCAGTA
CAGGCGGTCTGGAAAGAACA

Product: two component LuxR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 244; Mature: 243

Protein sequence:

>244_residues
MTADGRWEDPGCVRDTPNIKRAQAGPVPVSVLLVDDEELVRVGLRVILESGGEIDVVGEASDGSQVVPLIRENTPDVVIM
DIRMPRVDGLAAIREILADVADPPKILVLTTFELDDYVYEALRSGANGFLLKRARPAEIVQAVRTIVSSESLLFPEAIRY
MAGKRPNREAHETMRRAVLTDREADVLRLISEGLSNNEIANRLYLGAQTVKSHVSSVLAKLGARDRTQAVIIAYESGFVE
AGKP

Sequences:

>Translated_244_residues
MTADGRWEDPGCVRDTPNIKRAQAGPVPVSVLLVDDEELVRVGLRVILESGGEIDVVGEASDGSQVVPLIRENTPDVVIM
DIRMPRVDGLAAIREILADVADPPKILVLTTFELDDYVYEALRSGANGFLLKRARPAEIVQAVRTIVSSESLLFPEAIRY
MAGKRPNREAHETMRRAVLTDREADVLRLISEGLSNNEIANRLYLGAQTVKSHVSSVLAKLGARDRTQAVIIAYESGFVE
AGKP
>Mature_243_residues
TADGRWEDPGCVRDTPNIKRAQAGPVPVSVLLVDDEELVRVGLRVILESGGEIDVVGEASDGSQVVPLIRENTPDVVIMD
IRMPRVDGLAAIREILADVADPPKILVLTTFELDDYVYEALRSGANGFLLKRARPAEIVQAVRTIVSSESLLFPEAIRYM
AGKRPNREAHETMRRAVLTDREADVLRLISEGLSNNEIANRLYLGAQTVKSHVSSVLAKLGARDRTQAVIIAYESGFVEA
GKP

Specific function: Member of the two-component regulatory system liaS/liaR probably involved in response to a subset of cell wall-active antibiotics that interfere with the lipid II cycle in the cytoplasmic membrane (bacitracin, nisin, ramoplanin and vancomycin). Seems also

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788222, Length=213, Percent_Identity=37.0892018779343, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1786747, Length=195, Percent_Identity=31.2820512820513, Blast_Score=111, Evalue=5e-26,
Organism=Escherichia coli, GI1788521, Length=209, Percent_Identity=33.9712918660287, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1787473, Length=212, Percent_Identity=33.9622641509434, Blast_Score=95, Evalue=4e-21,
Organism=Escherichia coli, GI1790102, Length=198, Percent_Identity=30.8080808080808, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI87082052, Length=104, Percent_Identity=38.4615384615385, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788712, Length=196, Percent_Identity=23.9795918367347, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1788394, Length=200, Percent_Identity=28.5, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787277, Length=159, Percent_Identity=27.0440251572327, Blast_Score=62, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6322044, Length=124, Percent_Identity=31.4516129032258, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 26652; Mature: 26521

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTADGRWEDPGCVRDTPNIKRAQAGPVPVSVLLVDDEELVRVGLRVILESGGEIDVVGEA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCEEEEEEC
SDGSQVVPLIRENTPDVVIMDIRMPRVDGLAAIREILADVADPPKILVLTTFELDDYVYE
CCCCEEEHHEECCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEEEEECHHHHHHH
ALRSGANGFLLKRARPAEIVQAVRTIVSSESLLFPEAIRYMAGKRPNREAHETMRRAVLT
HHHCCCCCEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHC
DREADVLRLISEGLSNNEIANRLYLGAQTVKSHVSSVLAKLGARDRTQAVIIAYESGFVE
CCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCC
AGKP
CCCC
>Mature Secondary Structure 
TADGRWEDPGCVRDTPNIKRAQAGPVPVSVLLVDDEELVRVGLRVILESGGEIDVVGEA
CCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCEEEEEEC
SDGSQVVPLIRENTPDVVIMDIRMPRVDGLAAIREILADVADPPKILVLTTFELDDYVYE
CCCCEEEHHEECCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEEEEECHHHHHHH
ALRSGANGFLLKRARPAEIVQAVRTIVSSESLLFPEAIRYMAGKRPNREAHETMRRAVLT
HHHCCCCCEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHC
DREADVLRLISEGLSNNEIANRLYLGAQTVKSHVSSVLAKLGARDRTQAVIIAYESGFVE
CCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCC
AGKP
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9639930; 9384377 [H]