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The map label for this gene is sir [H]

Identifier: 158317847

GI number: 158317847

Start: 7431615

End: 7433375

Strand: Reverse

Name: sir [H]

Synonym: Franean1_6105

Alternate gene names: 158317847

Gene position: 7433375-7431615 (Counterclockwise)

Preceding gene: 158317848

Following gene: 158317846

Centisome position: 82.76

GC content: 71.66

Gene sequence:

>1761_bases
ATGACTTCTCCTGCTCGACCCCCGCGTGCCGCGCGCCCGCAAAGCCCGTCCCGACCCAAGGGGCAGGGCCAATGGGCGCT
GGGCTACTCCGAGCCGCTCAACCCCAACGAGCGGATGAAGAAGGACCAGGACGGCCTCGCCGTGCGCGAGCGCATCCTGA
ACGAGTACCCGCACACAGGGTTCGACGGGATCGACCCGCAGGATCTGCGCGGCCGGTTCCGCTGGTGGGGGCTCTACACC
CAGCGCCGGCCGGGGATCAGCGGCGGCCGCACCGCCGTCCTCGAGCCCGAGGAGCTCGACGACCGCTACTTCATGCAGCG
CATCCGCCTGGACGGCGGCCGGATGACCTCCGAACAGCTCCGGATCATCGCTGACATCTCCACCCGTTACGGGCGGGACG
TCGCCGACGTGACCGACCGCCAGAACATCCAGCTGCACTGGATCCGCATCGAGGACGTCCCGGCCATCTGGGGGGCGCTC
GAGGCCGCGGGGATGACCACCGAGGAGTCGTGCGGCGACACCCCGCGCGTCATCCTCGGCTGCCCGCTGGCCGGGGTGGA
CGCCGACGAGGTCATCGACGCCACCGGCGTCATCGACCAGGTGGTGGAGCGGTTCGTCGGCGACACCACGCTGTCGAACC
TGCCCCGCAAGTTCAAGAGCGCGATGTCGGGCTGCCCCGACCAGTGCACCCTGCACGAGATCAACGACATCGCCTTCGTC
GGTGTCGTGCACCCCGAGCTCGGCCCCGGGTACGACCTGTGGGTCGGCGGCGGGCTGTCGACGAACCCGAAGCTCGGCGT
GCGCCTGGGCACCTTCGTCACTCCGGAGCGGGTGCCCGAGGTGTGGCACGGCGTCATCCAGCTCTTCCGTGACTACGGCT
ACCGGCGGATGCGGACCAGGGCGCGGCTGAAGTTCCTCGTCGCCGACATGGGCGTCGAGTGGGTGCGCTCGACCCTGGAG
AAGGAGTACCTGGACGCGGCGCTGCCGGACGGCCCGGCGCCGCTGCCGCCCCGCAACGAGGGCCGCGACCACATCGGCGT
CCACCAGCAGCGTGACGGGTTCTTCGCGGTCGGCTTCGCGCCGCGCGCCGGCCGCCTCACCGGGACGACGCTCACCGCCG
TCGCGAACCTCGCCGACCGGTTCGGCCAGGGGCGGATCCGGGCGACCACCACCCAGAAGCTGGTGATCCTGGACGTCCCC
GGCGAGGATCTCGACGCGCTCGAGGCGGAGCTGGCCGCGCTCGACCTGGTCGTCCGGCCGAGCGTCTTCCGCCGCGGCAC
GATGGCCTGCACCGGCATCGAGTTCTGCAAGCTGGCGATCGTCGAGACCAAGGGCAACGCCCGCGACCTCATCGTCGAGC
TGGAGCGCCGGCTGCCCGACTTCGACGAGCCCATCGGCATCAACATCAACGGCTGCCCGAACGCCTGCGCGCGGTTCCAG
GTGGCCGACATCGGCCTCAAGGGCTCGCTCGTCCCGGACGACAACGGCGAGATGGTCGAGGGGTTCCAGGTCCACCTCGG
TGGCCACCTGGGCACCCGCTCCCGGCTGGGCCGGAAGTCACGTGGTCTCAAGGTGACCGCCGACGGCCTGGTCGACTACG
TCGTCGGCCTGCTGGAGACCTACCAGGAACACCGGGCCGACGGCGACACCTTCGCCGACTGGGCGGAACGCGCGGACGAG
GCGCTGCTGACCGGCGTCGGGTCCGGCTCCGCGTCGGCCGGCCCGGCGGCCGGCCGCCGGGCCGAGGGCGGCGCGACGTG
A

Upstream 100 bases:

>100_bases
ACCTGTTGCGCGTCTCCAGCGCCGGCTGTCGCCGCTCCCGCTGAACTCCGCCCGCGCCGGCGTTCGACGCCGTCGTGGAC
CCTGCTCACCGAGAGATCTC

Downstream 100 bases:

>100_bases
GCGCCCGCGCCGTCCCGTTCTACTGCCCCTACTGCGGTGACGAGGACCTCGTTCCCGAGCCCGCGCCGGGCGGGACGGGC
GAGGGCCACGGGCACTGGTC

Product: sulfite reductase (ferredoxin)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 586; Mature: 585

Protein sequence:

>586_residues
MTSPARPPRAARPQSPSRPKGQGQWALGYSEPLNPNERMKKDQDGLAVRERILNEYPHTGFDGIDPQDLRGRFRWWGLYT
QRRPGISGGRTAVLEPEELDDRYFMQRIRLDGGRMTSEQLRIIADISTRYGRDVADVTDRQNIQLHWIRIEDVPAIWGAL
EAAGMTTEESCGDTPRVILGCPLAGVDADEVIDATGVIDQVVERFVGDTTLSNLPRKFKSAMSGCPDQCTLHEINDIAFV
GVVHPELGPGYDLWVGGGLSTNPKLGVRLGTFVTPERVPEVWHGVIQLFRDYGYRRMRTRARLKFLVADMGVEWVRSTLE
KEYLDAALPDGPAPLPPRNEGRDHIGVHQQRDGFFAVGFAPRAGRLTGTTLTAVANLADRFGQGRIRATTTQKLVILDVP
GEDLDALEAELAALDLVVRPSVFRRGTMACTGIEFCKLAIVETKGNARDLIVELERRLPDFDEPIGININGCPNACARFQ
VADIGLKGSLVPDDNGEMVEGFQVHLGGHLGTRSRLGRKSRGLKVTADGLVDYVVGLLETYQEHRADGDTFADWAERADE
ALLTGVGSGSASAGPAAGRRAEGGAT

Sequences:

>Translated_586_residues
MTSPARPPRAARPQSPSRPKGQGQWALGYSEPLNPNERMKKDQDGLAVRERILNEYPHTGFDGIDPQDLRGRFRWWGLYT
QRRPGISGGRTAVLEPEELDDRYFMQRIRLDGGRMTSEQLRIIADISTRYGRDVADVTDRQNIQLHWIRIEDVPAIWGAL
EAAGMTTEESCGDTPRVILGCPLAGVDADEVIDATGVIDQVVERFVGDTTLSNLPRKFKSAMSGCPDQCTLHEINDIAFV
GVVHPELGPGYDLWVGGGLSTNPKLGVRLGTFVTPERVPEVWHGVIQLFRDYGYRRMRTRARLKFLVADMGVEWVRSTLE
KEYLDAALPDGPAPLPPRNEGRDHIGVHQQRDGFFAVGFAPRAGRLTGTTLTAVANLADRFGQGRIRATTTQKLVILDVP
GEDLDALEAELAALDLVVRPSVFRRGTMACTGIEFCKLAIVETKGNARDLIVELERRLPDFDEPIGININGCPNACARFQ
VADIGLKGSLVPDDNGEMVEGFQVHLGGHLGTRSRLGRKSRGLKVTADGLVDYVVGLLETYQEHRADGDTFADWAERADE
ALLTGVGSGSASAGPAAGRRAEGGAT
>Mature_585_residues
TSPARPPRAARPQSPSRPKGQGQWALGYSEPLNPNERMKKDQDGLAVRERILNEYPHTGFDGIDPQDLRGRFRWWGLYTQ
RRPGISGGRTAVLEPEELDDRYFMQRIRLDGGRMTSEQLRIIADISTRYGRDVADVTDRQNIQLHWIRIEDVPAIWGALE
AAGMTTEESCGDTPRVILGCPLAGVDADEVIDATGVIDQVVERFVGDTTLSNLPRKFKSAMSGCPDQCTLHEINDIAFVG
VVHPELGPGYDLWVGGGLSTNPKLGVRLGTFVTPERVPEVWHGVIQLFRDYGYRRMRTRARLKFLVADMGVEWVRSTLEK
EYLDAALPDGPAPLPPRNEGRDHIGVHQQRDGFFAVGFAPRAGRLTGTTLTAVANLADRFGQGRIRATTTQKLVILDVPG
EDLDALEAELAALDLVVRPSVFRRGTMACTGIEFCKLAIVETKGNARDLIVELERRLPDFDEPIGININGCPNACARFQV
ADIGLKGSLVPDDNGEMVEGFQVHLGGHLGTRSRLGRKSRGLKVTADGLVDYVVGLLETYQEHRADGDTFADWAERADEA
LLTGVGSGSASAGPAAGRRAEGGAT

Specific function: This Enzyme Catalyzes The 6-Electron Reduction Of Sulfite To Sulfide. This Is One Of Several Activities Required For The Biosynthesis Of L-Cysteine From Sulfate. [C]

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Escherichia coli, GI1789122, Length=575, Percent_Identity=26.4347826086956, Blast_Score=116, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6322597, Length=560, Percent_Identity=25.3571428571429, Blast_Score=130, Evalue=5e-31,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066 [H]

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: =1.8.7.1 [H]

Molecular weight: Translated: 64163; Mature: 64032

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSPARPPRAARPQSPSRPKGQGQWALGYSEPLNPNERMKKDQDGLAVRERILNEYPHTG
CCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCHHHHCCCCCHHHHHHHHHHHCCCCC
FDGIDPQDLRGRFRWWGLYTQRRPGISGGRTAVLEPEELDDRYFMQRIRLDGGRMTSEQL
CCCCCCHHHCCCEEEEEEEECCCCCCCCCCEEEECHHHCCHHHHHHHHHCCCCCCCHHHH
RIIADISTRYGRDVADVTDRQNIQLHWIRIEDVPAIWGALEAAGMTTEESCGDTPRVILG
HEEEEHHHHHCCCHHHHCCCCCEEEEEEEEECCCHHHHHHHHCCCCCHHHCCCCCCEEEE
CPLAGVDADEVIDATGVIDQVVERFVGDTTLSNLPRKFKSAMSGCPDQCTLHEINDIAFV
CCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCEECCCEEEE
GVVHPELGPGYDLWVGGGLSTNPKLGVRLGTFVTPERVPEVWHGVIQLFRDYGYRRMRTR
EEECCCCCCCEEEEECCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHCHHHHHHH
ARLKFLVADMGVEWVRSTLEKEYLDAALPDGPAPLPPRNEGRDHIGVHQQRDGFFAVGFA
HHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEECCCCEEEEECC
PRAGRLTGTTLTAVANLADRFGQGRIRATTTQKLVILDVPGEDLDALEAELAALDLVVRP
CCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHCCC
SVFRRGTMACTGIEFCKLAIVETKGNARDLIVELERRLPDFDEPIGININGCPNACARFQ
HHHHCCCCEECCCCEEEEEEEECCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHEEE
VADIGLKGSLVPDDNGEMVEGFQVHLGGHLGTRSRLGRKSRGLKVTADGLVDYVVGLLET
EEECCCCCCCCCCCCCCEECCEEEEECCCCCCHHHHCHHHCCCEEEHHHHHHHHHHHHHH
YQEHRADGDTFADWAERADEALLTGVGSGSASAGPAAGRRAEGGAT
HHHHCCCCCHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TSPARPPRAARPQSPSRPKGQGQWALGYSEPLNPNERMKKDQDGLAVRERILNEYPHTG
CCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCHHHHCCCCCHHHHHHHHHHHCCCCC
FDGIDPQDLRGRFRWWGLYTQRRPGISGGRTAVLEPEELDDRYFMQRIRLDGGRMTSEQL
CCCCCCHHHCCCEEEEEEEECCCCCCCCCCEEEECHHHCCHHHHHHHHHCCCCCCCHHHH
RIIADISTRYGRDVADVTDRQNIQLHWIRIEDVPAIWGALEAAGMTTEESCGDTPRVILG
HEEEEHHHHHCCCHHHHCCCCCEEEEEEEEECCCHHHHHHHHCCCCCHHHCCCCCCEEEE
CPLAGVDADEVIDATGVIDQVVERFVGDTTLSNLPRKFKSAMSGCPDQCTLHEINDIAFV
CCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCEECCCEEEE
GVVHPELGPGYDLWVGGGLSTNPKLGVRLGTFVTPERVPEVWHGVIQLFRDYGYRRMRTR
EEECCCCCCCEEEEECCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHCHHHHHHH
ARLKFLVADMGVEWVRSTLEKEYLDAALPDGPAPLPPRNEGRDHIGVHQQRDGFFAVGFA
HHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEECCCCEEEEECC
PRAGRLTGTTLTAVANLADRFGQGRIRATTTQKLVILDVPGEDLDALEAELAALDLVVRP
CCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHCCC
SVFRRGTMACTGIEFCKLAIVETKGNARDLIVELERRLPDFDEPIGININGCPNACARFQ
HHHHCCCCEECCCCEEEEEEEECCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHEEE
VADIGLKGSLVPDDNGEMVEGFQVHLGGHLGTRSRLGRKSRGLKVTADGLVDYVVGLLET
EEECCCCCCCCCCCCCCEECCEEEEECCCCCCHHHHCHHHCCCEEEHHHHHHHHHHHHHH
YQEHRADGDTFADWAERADEALLTGVGSGSASAGPAAGRRAEGGAT
HHHHCCCCCHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]