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The map label for this gene is purH [H]

Identifier: 158317716

GI number: 158317716

Start: 7278198

End: 7280684

Strand: Reverse

Name: purH [H]

Synonym: Franean1_5974

Alternate gene names: 158317716

Gene position: 7280684-7278198 (Counterclockwise)

Preceding gene: 158317717

Following gene: 158317713

Centisome position: 81.06

GC content: 74.47

Gene sequence:

>2487_bases
GTGCCCGCTCGCCTAGTCGTCCTCGCCTCGGGGGCCGGCACCACCCTGCAGGCTGTCCTCGAAGCCTGCGCGGACCCGGC
CTTCGGCGCGCGGGTCGTCGCGGTCGGCACCGACCGGCCGGACACGGGTGCGCAGCGGCGCGCGGAGGCGGTCGGGGTAC
CGGTGTTCACGGTGCGGCTCGAGGAGTGCGCCGATCGCGCCGCCTTCAACGACGCGACCGCCACGCGGATCGCCGAGCAC
ACGCCGGACCTGCTCGTCCTCGCCGGGTACATGAAGATTCTCGGCAGCCAGGTGATCGGCCGGTTCCCCACGGTGAACAC
CCATCCCTCACTGCTCCCGGCCTTCCCGGGCGCCCACGCCGTGCGCGACGCGCTCGCCGCCGGCGTCCGGGTCAGCGGGG
TGACCGTGCACTGGGTCGACGAGGGCGTCGACACCGGTCCGGTGATCGACCAGGCGGCCGTCCCGGTCGAGCCCACGGAT
GACGAGGACGCGCTGCGGGCACGCATCCAGGAGGTGGAACGGCGGCTGTTCGTAGCCGTCATCGGTCGTGTCGTCCGGCG
GGAGCTCCCGCTGGCGGGCGCACGCGCGGGTAGTACAGGAGCGGGGGAGGCGGCGATCGGCCCGGTGCTCGCTGCCGGCC
CGAGCGCCGGCCGTGATCAGGTGGAACCCGCCCGCGCGGGCGGTGGGCCGGCACTCGCCGGCCCTGCCGGCGGGAGCCAT
TCCGGTGGGGTCCACGCAGAGAAACATCATGCCGACGGAGGCATCGTGGAAGGTTCAGGAGGGGTCATGGCGGGGGAGTC
GAGCACGGGCGCGGGGCCTGGTGACACGAGCGGGGTCGCGGGTGTGGCAGGTGCGGCGGGCGCGGCAGGCGCCGCCCCGG
CCGGCGGCCGGCCGGCCGAGGGCTCGGCGGAGCTGGTGGCGACGGGCCACCGGAGGTTGCGCCGGGCCCTGGTGAGCGTC
TACGACAAGGCCGGTCTGGAGGAGCTCGCCGCCGCGTTCGTCGAGGCGGGGGTCGAGGTGGTCTCCACCGGTTCGACGGC
CGAGGTCCTGGCCCGCCACGGTGTGGCGGTCACCCCGGTCAGCACGGTCACCGGCTTCCCGGAGGTGCTCGGCGGCCGGG
TCAAGACGCTGCACCCGCGAGTGCACGCCGGTCTGTTGGCGGACCTGCGCAACGCCGAGCACGCCGCCGTCCTGGCGGAG
CTCGACATCGAGCCGTTCGACCTGCTCGTTGTAAACCTCTACCCGTTCCGCGAGACCGTCGCCTCGGGCGCGACCGAGGA
CGAGGCGATCGAGCAGATCGACATCGGCGGGCCGGCCATGCTGCGGGCGGCGGCGAAGAACCACGCCTCGGTCGCCGTGG
TGGTGTCGCCGCAGGACTACGGCGACCTGGCCGCGGCCGTTCGCGGGTCCGGCTATGACCTCCCCGCCCGGCGCCGGCTG
GCGGCCCGCGCCTTCGCGCACACCGCGTCCTACGACGCGGCGGTCGCCTCCTGGTTCGCCAGCGCGCTGGCCCCCGACGA
CACCGCCCGCGAGACCGGCTGGCCGGACATCCTCGCGGCCCAGTGGCACCGGTCCAGCGTCCTGCGGTACGGCGAGAACC
CGCACCAGCGGGCCGCGCTGTACGTGGGCGAGGACGGCAGCCCCGGGCTCGCGTCCGCGCGCCAGCTCCACGGCAAGCCG
ATGTCCTACAACAACTACACCGACACCGACGCGGCCTGGCGGTCGGTGTTCGACTTCGCGGACCCGGCCGTCGCGGTGAT
CAAGCATGCCAACCCGTGCGGCATCGCCGTCGGTGCCACCGTCGCCGAGGCGCACCGCAAGGCCCACGCCTGCGACCCGG
TGTCGGCGTTCGGCGGCGTGATCGCGGTGAACCGCCCGGTGAGTGTGGAGCTCGCCGAGCAGATCGCGGAGATCTTCACC
GAGGTCGTCGTGGCGCCCGACTACGAGCCCGGGGCCGTCGAGATCCTCGCCCGCAAGCCGTCGATCCGCCTGCTCGTCTG
CGCGCCGCCGACCCACTCGCGCGGGGTCGAGATGCGCCAGGTCAGCGGTGGGATGCTGCTGCAGTCACGGGACGCCCTCG
ACACCCCCGGCGACCACCCGTCGGGCTGGACGCTCGAGGCCGGCGCCCCGGTCGACGACAGCACCCTCGCCGACCTCGGC
TTCGCCTGGCGCGCGGTTCGCTCGGTGAAGTCGAACGCGATCCTGCTCGCCGCGGACAACGCGACCGTCGGGGTCGGCAT
GGGGCAGGTCAACCGGGTGGACGCGGCGCGCCTCGCCGTGACCCGCGCCGGCGACCGGGCGAAGGGCTCGGTGGCCGCCA
GCGACGCGTTCTTCCCGTTCCCCGACGGCTTCCAGGTCCTCGCCGACGCGGGGGTGCGGGCGGTTGTCGAGCCGGGTGGC
TCGGTGCGCGACGATCTCGTGATCGCGGCCGCGCGGGAGTCCGGTGTCGCGCTGTACTTCACCGGTGTCCGGCACTTCGC
CCACTGA

Upstream 100 bases:

>100_bases
GTCAGTACATTCGGACAGTTGCCGCCGTGGGTCGTGTCCGCGCGCGGCCGAGTTCTCGACCGGTCCGGTGCCGGCCGGCC
GACCACCCACGGAGGGTCCT

Downstream 100 bases:

>100_bases
GCGCCGCCCACTGAGCGTGGCCCACCGCGTCGTCCGCGGAGCGTCGCCCACCGGGCGTGGTCCGCTAGCACGTCGCACGG
GAGCGTCGGCGCTGACGCCC

Product: phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 828; Mature: 827

Protein sequence:

>828_residues
MPARLVVLASGAGTTLQAVLEACADPAFGARVVAVGTDRPDTGAQRRAEAVGVPVFTVRLEECADRAAFNDATATRIAEH
TPDLLVLAGYMKILGSQVIGRFPTVNTHPSLLPAFPGAHAVRDALAAGVRVSGVTVHWVDEGVDTGPVIDQAAVPVEPTD
DEDALRARIQEVERRLFVAVIGRVVRRELPLAGARAGSTGAGEAAIGPVLAAGPSAGRDQVEPARAGGGPALAGPAGGSH
SGGVHAEKHHADGGIVEGSGGVMAGESSTGAGPGDTSGVAGVAGAAGAAGAAPAGGRPAEGSAELVATGHRRLRRALVSV
YDKAGLEELAAAFVEAGVEVVSTGSTAEVLARHGVAVTPVSTVTGFPEVLGGRVKTLHPRVHAGLLADLRNAEHAAVLAE
LDIEPFDLLVVNLYPFRETVASGATEDEAIEQIDIGGPAMLRAAAKNHASVAVVVSPQDYGDLAAAVRGSGYDLPARRRL
AARAFAHTASYDAAVASWFASALAPDDTARETGWPDILAAQWHRSSVLRYGENPHQRAALYVGEDGSPGLASARQLHGKP
MSYNNYTDTDAAWRSVFDFADPAVAVIKHANPCGIAVGATVAEAHRKAHACDPVSAFGGVIAVNRPVSVELAEQIAEIFT
EVVVAPDYEPGAVEILARKPSIRLLVCAPPTHSRGVEMRQVSGGMLLQSRDALDTPGDHPSGWTLEAGAPVDDSTLADLG
FAWRAVRSVKSNAILLAADNATVGVGMGQVNRVDAARLAVTRAGDRAKGSVAASDAFFPFPDGFQVLADAGVRAVVEPGG
SVRDDLVIAAARESGVALYFTGVRHFAH

Sequences:

>Translated_828_residues
MPARLVVLASGAGTTLQAVLEACADPAFGARVVAVGTDRPDTGAQRRAEAVGVPVFTVRLEECADRAAFNDATATRIAEH
TPDLLVLAGYMKILGSQVIGRFPTVNTHPSLLPAFPGAHAVRDALAAGVRVSGVTVHWVDEGVDTGPVIDQAAVPVEPTD
DEDALRARIQEVERRLFVAVIGRVVRRELPLAGARAGSTGAGEAAIGPVLAAGPSAGRDQVEPARAGGGPALAGPAGGSH
SGGVHAEKHHADGGIVEGSGGVMAGESSTGAGPGDTSGVAGVAGAAGAAGAAPAGGRPAEGSAELVATGHRRLRRALVSV
YDKAGLEELAAAFVEAGVEVVSTGSTAEVLARHGVAVTPVSTVTGFPEVLGGRVKTLHPRVHAGLLADLRNAEHAAVLAE
LDIEPFDLLVVNLYPFRETVASGATEDEAIEQIDIGGPAMLRAAAKNHASVAVVVSPQDYGDLAAAVRGSGYDLPARRRL
AARAFAHTASYDAAVASWFASALAPDDTARETGWPDILAAQWHRSSVLRYGENPHQRAALYVGEDGSPGLASARQLHGKP
MSYNNYTDTDAAWRSVFDFADPAVAVIKHANPCGIAVGATVAEAHRKAHACDPVSAFGGVIAVNRPVSVELAEQIAEIFT
EVVVAPDYEPGAVEILARKPSIRLLVCAPPTHSRGVEMRQVSGGMLLQSRDALDTPGDHPSGWTLEAGAPVDDSTLADLG
FAWRAVRSVKSNAILLAADNATVGVGMGQVNRVDAARLAVTRAGDRAKGSVAASDAFFPFPDGFQVLADAGVRAVVEPGG
SVRDDLVIAAARESGVALYFTGVRHFAH
>Mature_827_residues
PARLVVLASGAGTTLQAVLEACADPAFGARVVAVGTDRPDTGAQRRAEAVGVPVFTVRLEECADRAAFNDATATRIAEHT
PDLLVLAGYMKILGSQVIGRFPTVNTHPSLLPAFPGAHAVRDALAAGVRVSGVTVHWVDEGVDTGPVIDQAAVPVEPTDD
EDALRARIQEVERRLFVAVIGRVVRRELPLAGARAGSTGAGEAAIGPVLAAGPSAGRDQVEPARAGGGPALAGPAGGSHS
GGVHAEKHHADGGIVEGSGGVMAGESSTGAGPGDTSGVAGVAGAAGAAGAAPAGGRPAEGSAELVATGHRRLRRALVSVY
DKAGLEELAAAFVEAGVEVVSTGSTAEVLARHGVAVTPVSTVTGFPEVLGGRVKTLHPRVHAGLLADLRNAEHAAVLAEL
DIEPFDLLVVNLYPFRETVASGATEDEAIEQIDIGGPAMLRAAAKNHASVAVVVSPQDYGDLAAAVRGSGYDLPARRRLA
ARAFAHTASYDAAVASWFASALAPDDTARETGWPDILAAQWHRSSVLRYGENPHQRAALYVGEDGSPGLASARQLHGKPM
SYNNYTDTDAAWRSVFDFADPAVAVIKHANPCGIAVGATVAEAHRKAHACDPVSAFGGVIAVNRPVSVELAEQIAEIFTE
VVVAPDYEPGAVEILARKPSIRLLVCAPPTHSRGVEMRQVSGGMLLQSRDALDTPGDHPSGWTLEAGAPVDDSTLADLGF
AWRAVRSVKSNAILLAADNATVGVGMGQVNRVDAARLAVTRAGDRAKGSVAASDAFFPFPDGFQVLADAGVRAVVEPGGS
VRDDLVIAAARESGVALYFTGVRHFAH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=485, Percent_Identity=36.4948453608247, Blast_Score=237, Evalue=4e-62,
Organism=Homo sapiens, GI4503915, Length=184, Percent_Identity=35.3260869565217, Blast_Score=117, Evalue=6e-26,
Organism=Homo sapiens, GI209869995, Length=184, Percent_Identity=35.3260869565217, Blast_Score=117, Evalue=6e-26,
Organism=Homo sapiens, GI209869993, Length=184, Percent_Identity=35.3260869565217, Blast_Score=117, Evalue=6e-26,
Organism=Escherichia coli, GI1790439, Length=530, Percent_Identity=45.2830188679245, Blast_Score=440, Evalue=1e-124,
Organism=Escherichia coli, GI1788846, Length=180, Percent_Identity=37.7777777777778, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1787483, Length=152, Percent_Identity=32.8947368421053, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71985564, Length=486, Percent_Identity=34.156378600823, Blast_Score=224, Evalue=2e-58,
Organism=Caenorhabditis elegans, GI17567511, Length=190, Percent_Identity=38.4210526315789, Blast_Score=126, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI71985574, Length=317, Percent_Identity=28.391167192429, Blast_Score=92, Evalue=9e-19,
Organism=Saccharomyces cerevisiae, GI6323768, Length=486, Percent_Identity=36.8312757201646, Blast_Score=245, Evalue=2e-65,
Organism=Saccharomyces cerevisiae, GI6323056, Length=481, Percent_Identity=35.966735966736, Blast_Score=239, Evalue=1e-63,
Organism=Drosophila melanogaster, GI24649832, Length=482, Percent_Identity=34.2323651452282, Blast_Score=214, Evalue=3e-55,
Organism=Drosophila melanogaster, GI24582400, Length=180, Percent_Identity=42.2222222222222, Blast_Score=133, Evalue=6e-31,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 84863; Mature: 84732

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPARLVVLASGAGTTLQAVLEACADPAFGARVVAVGTDRPDTGAQRRAEAVGVPVFTVRL
CCCEEEEEECCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHCCCCEEEEEH
EECADRAAFNDATATRIAEHTPDLLVLAGYMKILGSQVIGRFPTVNTHPSLLPAFPGAHA
HHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHH
VRDALAAGVRVSGVTVHWVDEGVDTGPVIDQAAVPVEPTDDEDALRARIQEVERRLFVAV
HHHHHHCCCEECCEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
IGRVVRRELPLAGARAGSTGAGEAAIGPVLAAGPSAGRDQVEPARAGGGPALAGPAGGSH
HHHHHHHHCCCCCCCCCCCCCCCHHHCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCC
SGGVHAEKHHADGGIVEGSGGVMAGESSTGAGPGDTSGVAGVAGAAGAAGAAPAGGRPAE
CCCCCCCCCCCCCCEEECCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GSAELVATGHRRLRRALVSVYDKAGLEELAAAFVEAGVEVVSTGSTAEVLARHGVAVTPV
CCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEECCCHHHHHHHCCEEEECH
STVTGFPEVLGGRVKTLHPRVHAGLLADLRNAEHAAVLAELDIEPFDLLVVNLYPFRETV
HHHCCCHHHHCCCEEEECCHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEEEECCHHHHH
ASGATEDEAIEQIDIGGPAMLRAAAKNHASVAVVVSPQDYGDLAAAVRGSGYDLPARRRL
HCCCCCHHHHHHCCCCCHHHHHHHHCCCCEEEEEECCCCCHHHHHHHCCCCCCCCHHHHH
AARAFAHTASYDAAVASWFASALAPDDTARETGWPDILAAQWHRSSVLRYGENPHQRAAL
HHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHCCCCHHHHHHHHHHHHHHCCCCCCCCEEE
YVGEDGSPGLASARQLHGKPMSYNNYTDTDAAWRSVFDFADPAVAVIKHANPCGIAVGAT
EECCCCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHCCCCCCCEEHHHH
VAEAHRKAHACDPVSAFGGVIAVNRPVSVELAEQIAEIFTEVVVAPDYEPGAVEILARKP
HHHHHHHHCCCCCHHHCCCEEEECCCCCHHHHHHHHHHHHHHEECCCCCCCCEEEEEECC
SIRLLVCAPPTHSRGVEMRQVSGGMLLQSRDALDTPGDHPSGWTLEAGAPVDDSTLADLG
CEEEEEECCCCCCCCCEEEECCCCEEEECCCCCCCCCCCCCCEEEECCCCCCCCHHHHHH
FAWRAVRSVKSNAILLAADNATVGVGMGQVNRVDAARLAVTRAGDRAKGSVAASDAFFPF
HHHHHHHHHCCCEEEEEECCCEEEECCCCCCHHHHHHHEEECCCCCCCCCEEECCCCCCC
PDGFQVLADAGVRAVVEPGGSVRDDLVIAAARESGVALYFTGVRHFAH
CCHHHHHHHCCCEEEECCCCCCCCCEEEEEECCCCEEEEEECHHHHCC
>Mature Secondary Structure 
PARLVVLASGAGTTLQAVLEACADPAFGARVVAVGTDRPDTGAQRRAEAVGVPVFTVRL
CCEEEEEECCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHCCCCEEEEEH
EECADRAAFNDATATRIAEHTPDLLVLAGYMKILGSQVIGRFPTVNTHPSLLPAFPGAHA
HHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHH
VRDALAAGVRVSGVTVHWVDEGVDTGPVIDQAAVPVEPTDDEDALRARIQEVERRLFVAV
HHHHHHCCCEECCEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
IGRVVRRELPLAGARAGSTGAGEAAIGPVLAAGPSAGRDQVEPARAGGGPALAGPAGGSH
HHHHHHHHCCCCCCCCCCCCCCCHHHCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCC
SGGVHAEKHHADGGIVEGSGGVMAGESSTGAGPGDTSGVAGVAGAAGAAGAAPAGGRPAE
CCCCCCCCCCCCCCEEECCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GSAELVATGHRRLRRALVSVYDKAGLEELAAAFVEAGVEVVSTGSTAEVLARHGVAVTPV
CCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEECCCHHHHHHHCCEEEECH
STVTGFPEVLGGRVKTLHPRVHAGLLADLRNAEHAAVLAELDIEPFDLLVVNLYPFRETV
HHHCCCHHHHCCCEEEECCHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEEEECCHHHHH
ASGATEDEAIEQIDIGGPAMLRAAAKNHASVAVVVSPQDYGDLAAAVRGSGYDLPARRRL
HCCCCCHHHHHHCCCCCHHHHHHHHCCCCEEEEEECCCCCHHHHHHHCCCCCCCCHHHHH
AARAFAHTASYDAAVASWFASALAPDDTARETGWPDILAAQWHRSSVLRYGENPHQRAAL
HHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHCCCCHHHHHHHHHHHHHHCCCCCCCCEEE
YVGEDGSPGLASARQLHGKPMSYNNYTDTDAAWRSVFDFADPAVAVIKHANPCGIAVGAT
EECCCCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHCCCCCCCEEHHHH
VAEAHRKAHACDPVSAFGGVIAVNRPVSVELAEQIAEIFTEVVVAPDYEPGAVEILARKP
HHHHHHHHCCCCCHHHCCCEEEECCCCCHHHHHHHHHHHHHHEECCCCCCCCEEEEEECC
SIRLLVCAPPTHSRGVEMRQVSGGMLLQSRDALDTPGDHPSGWTLEAGAPVDDSTLADLG
CEEEEEECCCCCCCCCEEEECCCCEEEECCCCCCCCCCCCCCEEEECCCCCCCCHHHHHH
FAWRAVRSVKSNAILLAADNATVGVGMGQVNRVDAARLAVTRAGDRAKGSVAASDAFFPF
HHHHHHHHHCCCEEEEEECCCEEEECCCCCCHHHHHHHEEECCCCCCCCCEEECCCCCCC
PDGFQVLADAGVRAVVEPGGSVRDDLVIAAARESGVALYFTGVRHFAH
CCHHHHHHHCCCEEEECCCCCCCCCEEEEEECCCCEEEEEECHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA