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The map label for this gene is dnaN [H]

Identifier: 158317615

GI number: 158317615

Start: 7124992

End: 7126206

Strand: Reverse

Name: dnaN [H]

Synonym: Franean1_5871

Alternate gene names: 158317615

Gene position: 7126206-7124992 (Counterclockwise)

Preceding gene: 158317620

Following gene: 158317610

Centisome position: 79.34

GC content: 75.64

Gene sequence:

>1215_bases
GTGGAACGCGACGTACTCGCCGACGCGACGGGCTGGGCCGCCCGGTACGTGCCGAAGCCCACCGCCGGCGCCCAGCAGGT
GCTGACAGGCCTGCTGCTGGAAGCCACCATGCCGACACCGGACCAGGCCGGCGACCAGGCCGGCGACCAGGCGACGGATC
AGACGACGGAGCAGGCACGGACGCAGGCGCCGGGCCCGGCGCTCACCGTCTCCGCGTTCGACTCCGAGGTCGCCGCGCGG
GCCCCGGTCGAGGCCGTCGTCACCGAGCCCGGGCGGGTCGTCGTGCCCGGGCGGCTGCTGGCGGATGTCGTGCGCAACCT
GCCCGACGCGCCGGTGGTCGTCGAGGCCACCGAGACCAGGGTGATCATCGAGTGCGGGGCGGCCCGGTTCCGCATCCCGG
CGCTCGCCGCCGAGGACTACCCGGTGCTGCCCGCGTTCCCGCCGCCGGTCGGCGAGGTGGACACGCTCGGCTTCGCCGCG
GCGGTCGCGCAGGTGGCCCCGGCCGCCGGCCGGGACGACGCGCTGCCGGTGCTCACCGCCGTCCGGCTCGAGATCGCCCG
GGGCGGGCTGACGATGGTCGCCACCGACCGGTACCGGCTCGCCGTCCGGACGATCCCGTGGCAGCCCACCGTCGACGAGC
CGGACGCGGTCGCGCACGTGCCGGCCCGGCTGCTCGCCGACATGGCGAAGCTGCCGACCACCGCGTCCCGGGTCGCGCTC
GGGATGGGCGTCGACGAGGCCGGGGTCCTGCGTTTCGGGCTGTCCGTGAACGGCCGGCAGACGATCGTCCGGCTGCTGGA
GGGGAGCTTCCCGAACTACCGCCGGCTGCTGCCGGAGACGTCGGAGCTCACCGTCACGGCGCCGACGGCGGTGCTCGCGG
CGGCCGTCCGCCGGGTCGCGCTGGTGGCGACGCGCACGGCGCCGCTGCGGTTCACGTTCTCCGCCGGCCAGGTCGTGGCC
GAGGCGGGGGACGGCGGGGGCGCCCAGGCGTCCGAGCTGGTGCCCGTGGAGTACGACGGCCCGGAGCTGTCGGTGCTGTT
CAACCCCGCGTTCCTGCTGGACGGCCTAGCGGCGGTCGAGGGCGACGAGGCCATCATCGGGTTCGCCACCGCCGACCCGG
AGATCGCCTCGAGCAAGCCGGCGGTCCTCACCGGCAAGGATGACGGTGAGGACTACCGCTACCTGCTCATGCCGATCCGC
ACCGGCACCGCCTGA

Upstream 100 bases:

>100_bases
CCTGGTGACAGGCCTGCCGCGCCGCCGCGAGCGGTGGGCGGTTTGCGGAATGCGGAGATTCGGCGTTGTCCGGCGCGACA
ATCCCTGCATGAGGTTCAGC

Downstream 100 bases:

>100_bases
CCGCTCCGGCGCACCCCCAGGGCGCGCCGGAGCGCCGGCTCAGGGTGCGGTCGGGGCCTGGGTCATCGTGCCGGCGCCGG
GAACCACCCCGCCCGCGGCG

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MERDVLADATGWAARYVPKPTAGAQQVLTGLLLEATMPTPDQAGDQAGDQATDQTTEQARTQAPGPALTVSAFDSEVAAR
APVEAVVTEPGRVVVPGRLLADVVRNLPDAPVVVEATETRVIIECGAARFRIPALAAEDYPVLPAFPPPVGEVDTLGFAA
AVAQVAPAAGRDDALPVLTAVRLEIARGGLTMVATDRYRLAVRTIPWQPTVDEPDAVAHVPARLLADMAKLPTTASRVAL
GMGVDEAGVLRFGLSVNGRQTIVRLLEGSFPNYRRLLPETSELTVTAPTAVLAAAVRRVALVATRTAPLRFTFSAGQVVA
EAGDGGGAQASELVPVEYDGPELSVLFNPAFLLDGLAAVEGDEAIIGFATADPEIASSKPAVLTGKDDGEDYRYLLMPIR
TGTA

Sequences:

>Translated_404_residues
MERDVLADATGWAARYVPKPTAGAQQVLTGLLLEATMPTPDQAGDQAGDQATDQTTEQARTQAPGPALTVSAFDSEVAAR
APVEAVVTEPGRVVVPGRLLADVVRNLPDAPVVVEATETRVIIECGAARFRIPALAAEDYPVLPAFPPPVGEVDTLGFAA
AVAQVAPAAGRDDALPVLTAVRLEIARGGLTMVATDRYRLAVRTIPWQPTVDEPDAVAHVPARLLADMAKLPTTASRVAL
GMGVDEAGVLRFGLSVNGRQTIVRLLEGSFPNYRRLLPETSELTVTAPTAVLAAAVRRVALVATRTAPLRFTFSAGQVVA
EAGDGGGAQASELVPVEYDGPELSVLFNPAFLLDGLAAVEGDEAIIGFATADPEIASSKPAVLTGKDDGEDYRYLLMPIR
TGTA
>Mature_404_residues
MERDVLADATGWAARYVPKPTAGAQQVLTGLLLEATMPTPDQAGDQAGDQATDQTTEQARTQAPGPALTVSAFDSEVAAR
APVEAVVTEPGRVVVPGRLLADVVRNLPDAPVVVEATETRVIIECGAARFRIPALAAEDYPVLPAFPPPVGEVDTLGFAA
AVAQVAPAAGRDDALPVLTAVRLEIARGGLTMVATDRYRLAVRTIPWQPTVDEPDAVAHVPARLLADMAKLPTTASRVAL
GMGVDEAGVLRFGLSVNGRQTIVRLLEGSFPNYRRLLPETSELTVTAPTAVLAAAVRRVALVATRTAPLRFTFSAGQVVA
EAGDGGGAQASELVPVEYDGPELSVLFNPAFLLDGLAAVEGDEAIIGFATADPEIASSKPAVLTGKDDGEDYRYLLMPIR
TGTA

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=349, Percent_Identity=26.3610315186246, Blast_Score=92, Evalue=6e-20,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 42108; Mature: 42108

Theoretical pI: Translated: 4.31; Mature: 4.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERDVLADATGWAARYVPKPTAGAQQVLTGLLLEATMPTPDQAGDQAGDQATDQTTEQAR
CCCCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHH
TQAPGPALTVSAFDSEVAARAPVEAVVTEPGRVVVPGRLLADVVRNLPDAPVVVEATETR
HCCCCCEEEEEECCCHHHHCCCCEEEEECCCCEEECHHHHHHHHHCCCCCCEEEEECCCE
VIIECGAARFRIPALAAEDYPVLPAFPPPVGEVDTLGFAAAVAQVAPAAGRDDALPVLTA
EEEEECCCEEECCEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHH
VRLEIARGGLTMVATDRYRLAVRTIPWQPTVDEPDAVAHVPARLLADMAKLPTTASRVAL
HHHHHHCCCEEEEEECCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHEEE
GMGVDEAGVLRFGLSVNGRQTIVRLLEGSFPNYRRLLPETSELTVTAPTAVLAAAVRRVA
ECCCCCCCEEEEECCCCCHHHHHHHHHCCCCHHHHHCCCCCCEEEECHHHHHHHHHHHHH
LVATRTAPLRFTFSAGQVVAEAGDGGGAQASELVPVEYDGPELSVLFNPAFLLDGLAAVE
HEEECCCCEEEEECCCEEEEECCCCCCCCCCCEEEEEECCCEEEEEECCHHHHCCHHHCC
GDEAIIGFATADPEIASSKPAVLTGKDDGEDYRYLLMPIRTGTA
CCCEEEEEEECCCCCCCCCCEEEECCCCCCCEEEEEEEECCCCC
>Mature Secondary Structure
MERDVLADATGWAARYVPKPTAGAQQVLTGLLLEATMPTPDQAGDQAGDQATDQTTEQAR
CCCCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHH
TQAPGPALTVSAFDSEVAARAPVEAVVTEPGRVVVPGRLLADVVRNLPDAPVVVEATETR
HCCCCCEEEEEECCCHHHHCCCCEEEEECCCCEEECHHHHHHHHHCCCCCCEEEEECCCE
VIIECGAARFRIPALAAEDYPVLPAFPPPVGEVDTLGFAAAVAQVAPAAGRDDALPVLTA
EEEEECCCEEECCEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHH
VRLEIARGGLTMVATDRYRLAVRTIPWQPTVDEPDAVAHVPARLLADMAKLPTTASRVAL
HHHHHHCCCEEEEEECCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHEEE
GMGVDEAGVLRFGLSVNGRQTIVRLLEGSFPNYRRLLPETSELTVTAPTAVLAAAVRRVA
ECCCCCCCEEEEECCCCCHHHHHHHHHCCCCHHHHHCCCCCCEEEECHHHHHHHHHHHHH
LVATRTAPLRFTFSAGQVVAEAGDGGGAQASELVPVEYDGPELSVLFNPAFLLDGLAAVE
HEEECCCCEEEEECCCEEEEECCCCCCCCCCCEEEEEECCCEEEEEECCHHHHCCHHHCC
GDEAIIGFATADPEIASSKPAVLTGKDDGEDYRYLLMPIRTGTA
CCCEEEEEEECCCCCCCCCCEEEECCCCCCCEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1577691; 12000953 [H]