Click here to switch to the map view.

The map label for this gene is gsiB [H]

Identifier: 158317308

GI number: 158317308

Start: 6731362

End: 6732930

Strand: Reverse

Name: gsiB [H]

Synonym: Franean1_5556

Alternate gene names: 158317308

Gene position: 6732930-6731362 (Counterclockwise)

Preceding gene: 158317312

Following gene: 158317300

Centisome position: 74.96

GC content: 65.9

Gene sequence:

>1569_bases
ATGGCGCGTAAGAAGCGATTACTCGGCGCGGCCGTGGTCTGCGTCGCTACCCTTGTCCTGGGAGCCTGCGGGGGCGGTGA
TTCTGACACGGCCAGCCCGGCCAGCGATGCCTCCAGCAAACCCGTTTCGGGTGGCGTCGCGCGAATCATCATGACGAGTG
ACCCGACCAGCCTGGACCCGGCGTCGCTGGCCAATCAGGCACCGATCACGGCGGTGCTGGGCAACGCCCTGTACGGCACG
TTGCTGACCACGGACGAGACCAGCAAGGTCGGCTACTCGATGGCCGAGTCCTTCACCACGACCGACGGCGGCGCCACCTT
CGAGCTGAAGCTGCGCCCTGACCTGGTCTTCTCCGACGGTACGCCCCTCAACGCCGCGGCTGTGAAGTTCAACTGGGACC
GCATCAAGGACCCGGCCACCGCGTCGTCGAGCCTGCCGGAAGCGGCAATGGTCGCCTCGACCGAAGTGATCGACGACCGC
ACGATGAAGGTCACGATGACCACGCCCGTCGCGGCGTTCGCGCAGGCGGTTGTCGGCACGGTTCTGAACTGGGTGGCCTC
GCCGGCAGCGCTGCAGAAGGGCAAGCAGTCCTTCGACGAGAAGCCGATCGGCGCGGGACCCTTCACCCTGCAGAGCTGGA
CCCGTCAGGCCGAGATCAAGCTCACGAAGAACCCACGCTACTGGGACGCGCCCAAGCCCTATCTCGACGGGATCACGCTG
CGCACGGTGCTCGACTCCAACCAGCGTTACAACACTCTGACCAGCGGCGGCGCTGATGTTTCCATCGAAACAAACTGGAT
CAACCTCGGGAAAGCTGAGGCCGCTGGCCTCCCGTCCGACCTGCTGCCGCTCAGTGGTGGAAACTTCCTGGCGCTCAACA
CGCGCCGAGCCCCGTTCAACGATATCCGGGCTCGGCAGGCCGTGTCCGCGGCGCTGGACATCGACGCGCTGAACCTGGCC
GCCTACAACGGGAAGGGCAGTGTCGCGGACACGCTGTTCACCGATGCCTCACCCTTCTACTCGAAGACGCAGCTGAGGTC
CACCGACCGGGCGAAGGCACAGCAGCTCTTCGACGAGCTGGCGGCCGAGGGAAAGCCGGTGTCGTTCACCTTCTCCAGCT
ATCCGACCAGTGAGAACAAGGCGATCGCGGAGAACGTCCAGGCCCAGCTCAGCAGCTTCAAGAACGTCAAGGCCGAGGTT
GCGATCATCGATTTCGCGAAGGGTGCCGCGCTGCGCTCGACCCACGACTTCGACATGGTCATCTCGTCGGCGGCCTTCCA
GGACCCCGAGCCGCGGCTGCTGGCGAACTTCACCGGGAACTCACCGGCCAACATGTCCGGTCTCGCGGACCCGGAACTGG
ATGCGGCCCTGCTGGCCGGCCGGACCGCGACCTCGGTGGCCGATCGTAAGGCGGCCTACGACAAGGTACAGGCGCGACTG
ACAGCGCTGACGCCGGTCATCTTCCTCATGCGATCGGCCCCCGGCGCGATCGCGGCCAAGAACGTCAACGGCCTCAGGCA
GTACGGCGCCGGCTCCCTGCTGCCCGAGGAGCTGTGGATCGAGAAGTAG

Upstream 100 bases:

>100_bases
GGACCCTATCCGGTCGGCTGATCCGTCGTCCCGGCGCGCCCTGAAGGGCGTTGACCGATCGATTCCATGATTCCGCCACG
TACATAACGAGAGGTCACTG

Downstream 100 bases:

>100_bases
CCCCCGCGGTGGCGCCCCGGGAACGCCGTGCATCTCACGACCCCGGGGCGTCAGCGTGTCCGCGGACGCCGGCCACGATG
GTGGCCGTCCTGCACTGTGA

Product: extracellular solute-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 522; Mature: 521

Protein sequence:

>522_residues
MARKKRLLGAAVVCVATLVLGACGGGDSDTASPASDASSKPVSGGVARIIMTSDPTSLDPASLANQAPITAVLGNALYGT
LLTTDETSKVGYSMAESFTTTDGGATFELKLRPDLVFSDGTPLNAAAVKFNWDRIKDPATASSSLPEAAMVASTEVIDDR
TMKVTMTTPVAAFAQAVVGTVLNWVASPAALQKGKQSFDEKPIGAGPFTLQSWTRQAEIKLTKNPRYWDAPKPYLDGITL
RTVLDSNQRYNTLTSGGADVSIETNWINLGKAEAAGLPSDLLPLSGGNFLALNTRRAPFNDIRARQAVSAALDIDALNLA
AYNGKGSVADTLFTDASPFYSKTQLRSTDRAKAQQLFDELAAEGKPVSFTFSSYPTSENKAIAENVQAQLSSFKNVKAEV
AIIDFAKGAALRSTHDFDMVISSAAFQDPEPRLLANFTGNSPANMSGLADPELDAALLAGRTATSVADRKAAYDKVQARL
TALTPVIFLMRSAPGAIAAKNVNGLRQYGAGSLLPEELWIEK

Sequences:

>Translated_522_residues
MARKKRLLGAAVVCVATLVLGACGGGDSDTASPASDASSKPVSGGVARIIMTSDPTSLDPASLANQAPITAVLGNALYGT
LLTTDETSKVGYSMAESFTTTDGGATFELKLRPDLVFSDGTPLNAAAVKFNWDRIKDPATASSSLPEAAMVASTEVIDDR
TMKVTMTTPVAAFAQAVVGTVLNWVASPAALQKGKQSFDEKPIGAGPFTLQSWTRQAEIKLTKNPRYWDAPKPYLDGITL
RTVLDSNQRYNTLTSGGADVSIETNWINLGKAEAAGLPSDLLPLSGGNFLALNTRRAPFNDIRARQAVSAALDIDALNLA
AYNGKGSVADTLFTDASPFYSKTQLRSTDRAKAQQLFDELAAEGKPVSFTFSSYPTSENKAIAENVQAQLSSFKNVKAEV
AIIDFAKGAALRSTHDFDMVISSAAFQDPEPRLLANFTGNSPANMSGLADPELDAALLAGRTATSVADRKAAYDKVQARL
TALTPVIFLMRSAPGAIAAKNVNGLRQYGAGSLLPEELWIEK
>Mature_521_residues
ARKKRLLGAAVVCVATLVLGACGGGDSDTASPASDASSKPVSGGVARIIMTSDPTSLDPASLANQAPITAVLGNALYGTL
LTTDETSKVGYSMAESFTTTDGGATFELKLRPDLVFSDGTPLNAAAVKFNWDRIKDPATASSSLPEAAMVASTEVIDDRT
MKVTMTTPVAAFAQAVVGTVLNWVASPAALQKGKQSFDEKPIGAGPFTLQSWTRQAEIKLTKNPRYWDAPKPYLDGITLR
TVLDSNQRYNTLTSGGADVSIETNWINLGKAEAAGLPSDLLPLSGGNFLALNTRRAPFNDIRARQAVSAALDIDALNLAA
YNGKGSVADTLFTDASPFYSKTQLRSTDRAKAQQLFDELAAEGKPVSFTFSSYPTSENKAIAENVQAQLSSFKNVKAEVA
IIDFAKGAALRSTHDFDMVISSAAFQDPEPRLLANFTGNSPANMSGLADPELDAALLAGRTATSVADRKAAYDKVQARLT
ALTPVIFLMRSAPGAIAAKNVNGLRQYGAGSLLPEELWIEK

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=416, Percent_Identity=28.8461538461538, Blast_Score=148, Evalue=1e-36,
Organism=Escherichia coli, GI1787495, Length=480, Percent_Identity=23.9583333333333, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1789397, Length=478, Percent_Identity=24.6861924686192, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1789966, Length=365, Percent_Identity=26.027397260274, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1787762, Length=480, Percent_Identity=23.3333333333333, Blast_Score=92, Evalue=8e-20,
Organism=Escherichia coli, GI1787551, Length=476, Percent_Identity=23.9495798319328, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI1789887, Length=469, Percent_Identity=24.5202558635394, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI87081878, Length=537, Percent_Identity=24.0223463687151, Blast_Score=87, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 55013; Mature: 54882

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARKKRLLGAAVVCVATLVLGACGGGDSDTASPASDASSKPVSGGVARIIMTSDPTSLDP
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCH
ASLANQAPITAVLGNALYGTLLTTDETSKVGYSMAESFTTTDGGATFELKLRPDLVFSDG
HHHCCCCCHHHHHHHHHEEEEEECCCCHHHHHHHHHHCCCCCCCEEEEEEECCCEEECCC
TPLNAAAVKFNWDRIKDPATASSSLPEAAMVASTEVIDDRTMKVTMTTPVAAFAQAVVGT
CCCCEEEEEEEHHHCCCCCCCCCCCCCHHHHHHHHEECCCEEEEEECCHHHHHHHHHHHH
VLNWVASPAALQKGKQSFDEKPIGAGPFTLQSWTRQAEIKLTKNPRYWDAPKPYLDGITL
HHHHHCCHHHHHHHHHHHCCCCCCCCCCHHHHHCCEEEEEECCCCCCCCCCCHHHCCEEE
RTVLDSNQRYNTLTSGGADVSIETNWINLGKAEAAGLPSDLLPLSGGNFLALNTRRAPFN
EEECCCCCCCCEECCCCCEEEEEECEEECCCHHHCCCCHHCCCCCCCCEEEEECCCCCHH
DIRARQAVSAALDIDALNLAAYNGKGSVADTLFTDASPFYSKTQLRSTDRAKAQQLFDEL
HHHHHHHHHHHHCCEEEEEEEECCCCCHHHHHHCCCCCCHHHHHHCCHHHHHHHHHHHHH
AAEGKPVSFTFSSYPTSENKAIAENVQAQLSSFKNVKAEVAIIDFAKGAALRSTHDFDMV
HHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCCCCCCCHHHH
ISSAAFQDPEPRLLANFTGNSPANMSGLADPELDAALLAGRTATSVADRKAAYDKVQARL
HHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHH
TALTPVIFLMRSAPGAIAAKNVNGLRQYGAGSLLPEELWIEK
HHHHHHHHHHHCCCCCEEECCCHHHHHHCCCCCCCHHHCCCC
>Mature Secondary Structure 
ARKKRLLGAAVVCVATLVLGACGGGDSDTASPASDASSKPVSGGVARIIMTSDPTSLDP
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCH
ASLANQAPITAVLGNALYGTLLTTDETSKVGYSMAESFTTTDGGATFELKLRPDLVFSDG
HHHCCCCCHHHHHHHHHEEEEEECCCCHHHHHHHHHHCCCCCCCEEEEEEECCCEEECCC
TPLNAAAVKFNWDRIKDPATASSSLPEAAMVASTEVIDDRTMKVTMTTPVAAFAQAVVGT
CCCCEEEEEEEHHHCCCCCCCCCCCCCHHHHHHHHEECCCEEEEEECCHHHHHHHHHHHH
VLNWVASPAALQKGKQSFDEKPIGAGPFTLQSWTRQAEIKLTKNPRYWDAPKPYLDGITL
HHHHHCCHHHHHHHHHHHCCCCCCCCCCHHHHHCCEEEEEECCCCCCCCCCCHHHCCEEE
RTVLDSNQRYNTLTSGGADVSIETNWINLGKAEAAGLPSDLLPLSGGNFLALNTRRAPFN
EEECCCCCCCCEECCCCCEEEEEECEEECCCHHHCCCCHHCCCCCCCCEEEEECCCCCHH
DIRARQAVSAALDIDALNLAAYNGKGSVADTLFTDASPFYSKTQLRSTDRAKAQQLFDEL
HHHHHHHHHHHHCCEEEEEEEECCCCCHHHHHHCCCCCCHHHHHHCCHHHHHHHHHHHHH
AAEGKPVSFTFSSYPTSENKAIAENVQAQLSSFKNVKAEVAIIDFAKGAALRSTHDFDMV
HHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCCCCCCCHHHH
ISSAAFQDPEPRLLANFTGNSPANMSGLADPELDAALLAGRTATSVADRKAAYDKVQARL
HHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHH
TALTPVIFLMRSAPGAIAAKNVNGLRQYGAGSLLPEELWIEK
HHHHHHHHHHHCCCCCEEECCCHHHHHHCCCCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA