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The map label for this gene is actII-2 [H]

Identifier: 158317306

GI number: 158317306

Start: 6725738

End: 6727390

Strand: Direct

Name: actII-2 [H]

Synonym: Franean1_5554

Alternate gene names: 158317306

Gene position: 6725738-6727390 (Clockwise)

Preceding gene: 158317305

Following gene: 158317307

Centisome position: 74.88

GC content: 70.66

Gene sequence:

>1653_bases
ATGACACCCCGGGCAGCCGGGATTCAGAGCCCTGGTGCCGGGCCGGAGGCCGCTTCGGCCGGCCTGGTCGTTCTTCCCAC
ACTGGCGGCGGCGCAGTTCCTCATGACCCTGGACAGCTCGGTCATGAACGTGTCGATCGCCACCGTGGCCGCGGACATCG
GTACCACGGTGACCGGAATTCAGACCGCCATCACCTTCTACACCCTGGTGATGGCCGCGTTCATGATTACCGGCGGCAGG
CTGGGGCAGCTCTTCGGGCATCGGCGTGTGTTCACCATCGGCTGCGTCGTCTACGGCTGTGGGTCGCTGACGACGTCCGT
CGCCGGGAATCTCGCCGTGCTCATGTTCGGCTGGTCGTTCCTCGAAGGAATCGGCGCCGCGCTGATCATGCCCGCGGTCG
TCGCTCTGGTCGCGTCGAACTTCGCCTCGGCGCAGCGACCGCGCGCCTACGGGCTGGTCGCCGCCGCCGGCGCGATCGCG
GTCGCGGCGGGTCCGCTCGTCGGCGGCCTGTTCACCACCTACCTGTCCTGGCGCTGGGTCTTCGCAGGCGAGGTCCTCGT
GGTGGCCGTCATCCTGTGGCTGACCCGGGGGATGGCGGACACGCCTCCGACCGCGCAGGGACGTCTCGACATCGTGGGCA
CCGTGCTGTCCGCGGCCGGCCTGGCGCTGATCGTGTACGGCGTCCTCCGCTCGGGGAGCTGGGGCCTGGTCCGGCCGGCG
CCCGGCGCGCCCGTCTGGTTGGGGCTCTCACCCGTGATCTGGCTGGTCCTCGCGGGCGGAACCATCCTGTTGCTCTTCGT
CCGGTGGCAGGATCACCGGCTGGCCCGCGGCGCCGCCGCGCTGCTCGATCCGGTGCTGCTGCGGAACCGGACGTTCCGGG
CCGGGCTCACGTCGTTCTTCTTCCAGTATCTGCTCCAGGCGGGGCTCTTCTTCGTGGTACCGCTGTATCTGTCGGTGGCG
CTCGGGCTCTCGGCGGTCGCGACCGGCGTGCGTCTGCTGCCGTTGTCCATCGCCCTGCTGGTGGCCGCCGTCGGCATTCC
CAAAGCCCTCCCGCACGTCTCACCGCGACGCATCGTCCGTGGTGGTTTCCTCTCCCTGTTCGCCGGGACAACGATCCTGG
TCGCCGCGCTTGACGCCGGCGCCGGACCGGAGATCGTGACCTGGCCGATGCTGCTCGCCGGCCTCGGGGTGGGCGCGCTC
GCGTCCCAGCTCGGCAGCGTCACCGTGTCCGCGGTCGCCGACGAGCACACCGGCGAGGTCGGTGGGGTGCAGAACACGGT
GACAAACCTGGGCGCGTCGATCGGTACCGCGGCAGCTGGGGCAGTTCTCATCTCCGCGCTGACCTCGTCGTTCCTCACCG
GCATACGGCACGACCCCGCCGTACCGGCGGAGCTGAGCACGCAGGCCGAGGTGCGCCTGGCCGAGGGCGTCCCCTTCCTG
TCCGACCGGGACCTGAAGATCCGGATCGACGAGGCCGGCGTCCCGCCGGACACCGCCAAGGTGATCACCGCCACCAACGC
GGACGCCCGCATCGACGGCCTGCGTGCGGCTCTGTCGCTGCTCGCCGCCATCGCCCTGGCCGCGATGTTCCTCACCCGCC
AGATCCCGGACCGGCAATCCGCTCCGGTGCCGGTTTCCGGCCGGGTCACCTGA

Upstream 100 bases:

>100_bases
CCGCTCACACCAAGGCAGAAATGCGAACCAAAAAGGGAACCGGCGATGCGGCCGGGTGCGGAGATCCTGACGCCACCAGG
CGTCGGCCCGGGCGCGAACG

Downstream 100 bases:

>100_bases
CCGTTCAGAGCCGACGGCCAGACGTACATCACGGTCACCGAAAGCAGGATTCGGCTGTGGGACATCACCGGCCCTCGTCT
GCTGGTTACGGGCCGCCGTG

Product: major facilitator transporter

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 550; Mature: 549

Protein sequence:

>550_residues
MTPRAAGIQSPGAGPEAASAGLVVLPTLAAAQFLMTLDSSVMNVSIATVAADIGTTVTGIQTAITFYTLVMAAFMITGGR
LGQLFGHRRVFTIGCVVYGCGSLTTSVAGNLAVLMFGWSFLEGIGAALIMPAVVALVASNFASAQRPRAYGLVAAAGAIA
VAAGPLVGGLFTTYLSWRWVFAGEVLVVAVILWLTRGMADTPPTAQGRLDIVGTVLSAAGLALIVYGVLRSGSWGLVRPA
PGAPVWLGLSPVIWLVLAGGTILLLFVRWQDHRLARGAAALLDPVLLRNRTFRAGLTSFFFQYLLQAGLFFVVPLYLSVA
LGLSAVATGVRLLPLSIALLVAAVGIPKALPHVSPRRIVRGGFLSLFAGTTILVAALDAGAGPEIVTWPMLLAGLGVGAL
ASQLGSVTVSAVADEHTGEVGGVQNTVTNLGASIGTAAAGAVLISALTSSFLTGIRHDPAVPAELSTQAEVRLAEGVPFL
SDRDLKIRIDEAGVPPDTAKVITATNADARIDGLRAALSLLAAIALAAMFLTRQIPDRQSAPVPVSGRVT

Sequences:

>Translated_550_residues
MTPRAAGIQSPGAGPEAASAGLVVLPTLAAAQFLMTLDSSVMNVSIATVAADIGTTVTGIQTAITFYTLVMAAFMITGGR
LGQLFGHRRVFTIGCVVYGCGSLTTSVAGNLAVLMFGWSFLEGIGAALIMPAVVALVASNFASAQRPRAYGLVAAAGAIA
VAAGPLVGGLFTTYLSWRWVFAGEVLVVAVILWLTRGMADTPPTAQGRLDIVGTVLSAAGLALIVYGVLRSGSWGLVRPA
PGAPVWLGLSPVIWLVLAGGTILLLFVRWQDHRLARGAAALLDPVLLRNRTFRAGLTSFFFQYLLQAGLFFVVPLYLSVA
LGLSAVATGVRLLPLSIALLVAAVGIPKALPHVSPRRIVRGGFLSLFAGTTILVAALDAGAGPEIVTWPMLLAGLGVGAL
ASQLGSVTVSAVADEHTGEVGGVQNTVTNLGASIGTAAAGAVLISALTSSFLTGIRHDPAVPAELSTQAEVRLAEGVPFL
SDRDLKIRIDEAGVPPDTAKVITATNADARIDGLRAALSLLAAIALAAMFLTRQIPDRQSAPVPVSGRVT
>Mature_549_residues
TPRAAGIQSPGAGPEAASAGLVVLPTLAAAQFLMTLDSSVMNVSIATVAADIGTTVTGIQTAITFYTLVMAAFMITGGRL
GQLFGHRRVFTIGCVVYGCGSLTTSVAGNLAVLMFGWSFLEGIGAALIMPAVVALVASNFASAQRPRAYGLVAAAGAIAV
AAGPLVGGLFTTYLSWRWVFAGEVLVVAVILWLTRGMADTPPTAQGRLDIVGTVLSAAGLALIVYGVLRSGSWGLVRPAP
GAPVWLGLSPVIWLVLAGGTILLLFVRWQDHRLARGAAALLDPVLLRNRTFRAGLTSFFFQYLLQAGLFFVVPLYLSVAL
GLSAVATGVRLLPLSIALLVAAVGIPKALPHVSPRRIVRGGFLSLFAGTTILVAALDAGAGPEIVTWPMLLAGLGVGALA
SQLGSVTVSAVADEHTGEVGGVQNTVTNLGASIGTAAAGAVLISALTSSFLTGIRHDPAVPAELSTQAEVRLAEGVPFLS
DRDLKIRIDEAGVPPDTAKVITATNADARIDGLRAALSLLAAIALAAMFLTRQIPDRQSAPVPVSGRVT

Specific function: Promotes the efflux of actinorhodin [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=428, Percent_Identity=24.7663551401869, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI1788710, Length=419, Percent_Identity=24.582338902148, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1789042, Length=415, Percent_Identity=23.3734939759036, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI87081983, Length=419, Percent_Identity=25.7756563245823, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1788392, Length=416, Percent_Identity=24.0384615384615, Blast_Score=74, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6322958, Length=500, Percent_Identity=22.4, Blast_Score=70, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6325455, Length=339, Percent_Identity=25.9587020648968, Blast_Score=68, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 56316; Mature: 56185

Theoretical pI: Translated: 10.09; Mature: 10.09

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCCCC
>Mature Secondary Structure 
TPRAAGIQSPGAGPEAASAGLVVLPTLAAAQFLMTLDSSVMNVSIATVAADIGTTVTGI
CCCCCCCCCCCCCCCHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
QTAITFYTLVMAAFMITGGRLGQLFGHRRVFTIGCVVYGCGSLTTSVAGNLAVLMFGWSF
HHHHHHHHHHHHHHHHCCCHHHHHHCCCHHEEHHHHHHCCCHHHHHHHCCHHHHHHHHHH
LEGIGAALIMPAVVALVASNFASAQRPRAYGLVAAAGAIAVAAGPLVGGLFTTYLSWRWV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FAGEVLVVAVILWLTRGMADTPPTAQGRLDIVGTVLSAAGLALIVYGVLRSGSWGLVRPA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
PGAPVWLGLSPVIWLVLAGGTILLLFVRWQDHRLARGAAALLDPVLLRNRTFRAGLTSFF
CCCCEEECHHHHHHHHHHCCHHEEEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
FQYLLQAGLFFVVPLYLSVALGLSAVATGVRLLPLSIALLVAAVGIPKALPHVSPRRIVR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCHHHHHH
GGFLSLFAGTTILVAALDAGAGPEIVTWPMLLAGLGVGALASQLGSVTVSAVADEHTGEV
HHHHHHHHHHHHEEHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHCCEEHHHHHCCCCCCC
GGVQNTVTNLGASIGTAAAGAVLISALTSSFLTGIRHDPAVPAELSTQAEVRLAEGVPFL
CCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHEEHHCCCCCC
SDRDLKIRIDEAGVPPDTAKVITATNADARIDGLRAALSLLAAIALAAMFLTRQIPDRQS
CCCCCEEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
APVPVSGRVT
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1878971; 12000953 [H]