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The map label for this gene is nfi [H]

Identifier: 158317185

GI number: 158317185

Start: 6571191

End: 6571868

Strand: Reverse

Name: nfi [H]

Synonym: Franean1_5432

Alternate gene names: 158317185

Gene position: 6571868-6571191 (Counterclockwise)

Preceding gene: 158317186

Following gene: 158317179

Centisome position: 73.17

GC content: 69.47

Gene sequence:

>678_bases
ATGAGGCAGGTCGAGGACGCTGAGGCGCTGCAGGCCGAGCTGTGTCGGCTTGTCCAGGCTGACGCATCCGGCCCGGACTC
GGTCACGACGGCGGCGGGTTGTGACGTCGCGTACGACTCCGACTCCGATCTGGTTGCGGCCGCGGTCGTCGTCCTCGCCG
CGCCGGGCTGGGAGGTGATCGCATCGGCTACGGCGGTAGGTCGGGCGGCGTTTCCCTACATTCCGGGTCTGCTGTCGTTC
CGGGAAATGCCGCCGCTGCTCGAAGCGTGGAAAACCGTTCTGCCGCAGTTGGCTGCGCCGCCAGAGCTGCTGGTGTGCGA
CGGGCATGGCATCGCACACCCCCGACGGTTCGGACTGGCCTGTCATCTCGGTGTCACCCTGAACCTGCCGACGGTCGGCG
TGGCAAAGACCCAGTTTGTCGGCGAGCACTCGCCGCCTGGGCCGCGTCGGGGAGACCGAACGGCGATCATTCTCGACGGC
GAGCCGGTGGGAGCCGCGCTGCGGACCCGCGACGGTGTCCGCGAGGTGTACGTCTCGGTCGGGCACCGCACCAACCTTGA
CTCCGCCTGCCGGTGGGTACTGGAGCTGTGCCCCAGGTACCGGCTGCCGGAGACGACCCGGGCGGCCGATCAGCTCAGCC
GAAGGGCGCTTGCCGAAGCCCAGACCGCGTCTCGCTGA

Upstream 100 bases:

>100_bases
CCTTCTAACCGATCGTGCCGCGGGACTTTCCGTCGTCACAAAGCTGCTGCCGGGCTGAACCAGACCAGCTCCGCGAGATT
GAATAACGCAGGGGGTTGCG

Downstream 100 bases:

>100_bases
GGGAGCATGGCCGGTGCGGCCCGGCCGACGGCGCACAGCAAACAGTTCGGCGACGCACCAGTTCGGATGCTGGCTCCCAC
CATCAGGTCAGGACGCGACC

Product: deoxyribonuclease V

Products: NA

Alternate protein names: Deoxyinosine 3'endonuclease; Deoxyribonuclease V; DNase V [H]

Number of amino acids: Translated: 225; Mature: 225

Protein sequence:

>225_residues
MRQVEDAEALQAELCRLVQADASGPDSVTTAAGCDVAYDSDSDLVAAAVVVLAAPGWEVIASATAVGRAAFPYIPGLLSF
REMPPLLEAWKTVLPQLAAPPELLVCDGHGIAHPRRFGLACHLGVTLNLPTVGVAKTQFVGEHSPPGPRRGDRTAIILDG
EPVGAALRTRDGVREVYVSVGHRTNLDSACRWVLELCPRYRLPETTRAADQLSRRALAEAQTASR

Sequences:

>Translated_225_residues
MRQVEDAEALQAELCRLVQADASGPDSVTTAAGCDVAYDSDSDLVAAAVVVLAAPGWEVIASATAVGRAAFPYIPGLLSF
REMPPLLEAWKTVLPQLAAPPELLVCDGHGIAHPRRFGLACHLGVTLNLPTVGVAKTQFVGEHSPPGPRRGDRTAIILDG
EPVGAALRTRDGVREVYVSVGHRTNLDSACRWVLELCPRYRLPETTRAADQLSRRALAEAQTASR
>Mature_225_residues
MRQVEDAEALQAELCRLVQADASGPDSVTTAAGCDVAYDSDSDLVAAAVVVLAAPGWEVIASATAVGRAAFPYIPGLLSF
REMPPLLEAWKTVLPQLAAPPELLVCDGHGIAHPRRFGLACHLGVTLNLPTVGVAKTQFVGEHSPPGPRRGDRTAIILDG
EPVGAALRTRDGVREVYVSVGHRTNLDSACRWVLELCPRYRLPETTRAADQLSRRALAEAQTASR

Specific function: Selectively cleaves double-stranded DNA at the second phosphodiester bond 3' to a deoxyinosine leaving behind the intact lesion on the nicked DNA. Acts in DNA repair [H]

COG id: COG1515

COG function: function code L; Deoxyinosine 3'endonuclease (endonuclease V)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the endonuclease V family [H]

Homologues:

Organism=Homo sapiens, GI31542775, Length=203, Percent_Identity=37.9310344827586, Blast_Score=112, Evalue=2e-25,
Organism=Homo sapiens, GI257467548, Length=131, Percent_Identity=41.2213740458015, Blast_Score=82, Evalue=3e-16,
Organism=Homo sapiens, GI257467550, Length=131, Percent_Identity=41.2213740458015, Blast_Score=81, Evalue=6e-16,
Organism=Escherichia coli, GI87082357, Length=201, Percent_Identity=37.8109452736318, Blast_Score=143, Evalue=1e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007581 [H]

Pfam domain/function: PF04493 Endonuclease_5 [H]

EC number: =3.1.21.7 [H]

Molecular weight: Translated: 23951; Mature: 23951

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQVEDAEALQAELCRLVQADASGPDSVTTAAGCDVAYDSDSDLVAAAVVVLAAPGWEVI
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCEEECCCCHHHHHHHHHHCCCCHHHH
ASATAVGRAAFPYIPGLLSFREMPPLLEAWKTVLPQLAAPPELLVCDGHGIAHPRRFGLA
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHCEE
CHLGVTLNLPTVGVAKTQFVGEHSPPGPRRGDRTAIILDGEPVGAALRTRDGVREVYVSV
EEEEEEECCCCCCCHHHHHCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHC
GHRTNLDSACRWVLELCPRYRLPETTRAADQLSRRALAEAQTASR
CCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRQVEDAEALQAELCRLVQADASGPDSVTTAAGCDVAYDSDSDLVAAAVVVLAAPGWEVI
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCEEECCCCHHHHHHHHHHCCCCHHHH
ASATAVGRAAFPYIPGLLSFREMPPLLEAWKTVLPQLAAPPELLVCDGHGIAHPRRFGLA
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHCEE
CHLGVTLNLPTVGVAKTQFVGEHSPPGPRRGDRTAIILDGEPVGAALRTRDGVREVYVSV
EEEEEEECCCCCCCHHHHHCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHC
GHRTNLDSACRWVLELCPRYRLPETTRAADQLSRRALAEAQTASR
CCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA