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The map label for this gene is lcfB [H]

Identifier: 158317182

GI number: 158317182

Start: 6567871

End: 6569448

Strand: Direct

Name: lcfB [H]

Synonym: Franean1_5429

Alternate gene names: 158317182

Gene position: 6567871-6569448 (Clockwise)

Preceding gene: 158317181

Following gene: 158317183

Centisome position: 73.12

GC content: 73.07

Gene sequence:

>1578_bases
ATGACGTCGACCGGCGACGAGATCCGACCGACCATCCCTGACCTGTTGGGGCGCTGCGCTCGGGAGTTCGGCTCCGCCGA
CTACATCGTCTCCCTCACCGACCGGCTGACCTACGCCGAGGCGGAGGAGCAGTCCGCCCGGGTGGCCCGGTGGCTGTTGC
ACGAGGGCGTCGGTAAGGGCACCCGGGTGGGCCTGTTCTTCCCCAGCGGCGTCGAGTGGGCCCTCTGGTGGCTGGCCGTG
AGCCGGATCGGGGCCGTGGCCGTTCCGCTCAGCACCCTGTACCCGCCGGCGGAGATCGCCAAGGTCGTGCGGCTGGCCGA
TGTGCAGCTCCTGGTGGCACCGACGACCGTGCTGCGGATCGACGTCGCGCAGCGGTTCGAGGCGGCGTTCCCCGAGCTGG
CCGGGCAGCTGGCCGGGCAGCCGGCCGGCCAGCTCGAGCTGGCGGGCGCGCCGTACCTGCGGCGGATCGTGCTAACCGGC
CAGACGGACCGGGGCTGGGCCACCCGGTGGGATCCGCGGGACCCGCCGCTGGTGCGGGCCGAGCTGCTCGCCGCGGTGCA
GACCGAGGTCACCCCGGCCGACCTGGCGATCATGGTTCACACCTCCGGTTCCACCGCCGACCCGAAGGGCGTGCTGCACA
CGCACGGCACGCTGGTGCGCCAGACCTCCACCTGGCCGGCGGCGATCCGCGGGCTCACCGGCGTCGACCACGCGCCGCGC
ATCCTGTGTGCCATGCCGTTCTTCTGGATCGGCGGGATCCTGGCCGCGACCGGAGCTCTGCACGCACCCGTCGCGGTCCT
GGTGCTGGCGCGGCTGGAAGCCGGGCCGGCCCTCGACCTCGCCGAACGGGAACGGGCGAACGGCGTCGTCGGATGGCCCG
CGTTCACCCAGCAGCTGCGGCTGCACCCGTCCTTCCCCAGCCGGGACCTGCGCAGCGCCCCCGCGCTGCGGGAGGGGCCG
GTGGACCTCGCGATGGCGGGCGTCCCGGACGGCCATCCGATCCACCGCAGCCTGACCGAGTCCGGCGGCAGCTTCGCGTT
CACCGAGACCGCGATCGTCGACGCCGCCGGCGAGCGCGTCCCGGACGGGACCGTCGGTGAGCTGCTCATCCGTGGCATCG
GCTCGATGGCCGGCTACAACAAGCGTGAGCGCGCGGAGGTCTTCGACGCGGACGGCTGGTACCACACCAGCGACCGCGTC
TACCGCAGGACGGGCGACCCGCGGCTGTTCTACGTCGGCCGGGACAGCGAGCTCGTCAAGGTCGCCGGTTCGAACGTGGC
ACCGCGCGAGGTCGAGGCCGTCATCGAGGAGTTCCCCGAGGTCGCGCACTGTGTCGTGACCGGTGTCGAGCATCCGACCC
GCGGCGAGGAGGTGTGCGCGGTCATCGTTCCGGCCGGCACGACCGGCACGGACGTCGACGTGGACGGTCTGGCCGCGCGC
ACCCGTACGCTCCTGTCCAGCTACAAGGTTCCGACCCGGTGGATCGTCGCCGCGGACGACGAGGTGCCGGCCCTGCCGAG
CGGCAAGCCGGACCGCCGCGGCCTGCGCACACTGATCGAGGACGGCCGACTGAAGTAG

Upstream 100 bases:

>100_bases
CGGCTCCGCCATCGCGTAGGCCCCGCCAGGTGCGTAGGCCCCGTCGTCGCGTCGGGGCCGCACCTGGCGCCGCCACCGGC
TGAAGGACGGAGCGAGGGCG

Downstream 100 bases:

>100_bases
GCGCGCGCCGCGGGGCTGGCTGAGCTCGTCCACCCCGAGATCGGGTCTGTGGTCAGGGGTGAGCACGGCCGCGTCGGCGT
GGCGGCATGTGGGTTGAGGC

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 525; Mature: 524

Protein sequence:

>525_residues
MTSTGDEIRPTIPDLLGRCAREFGSADYIVSLTDRLTYAEAEEQSARVARWLLHEGVGKGTRVGLFFPSGVEWALWWLAV
SRIGAVAVPLSTLYPPAEIAKVVRLADVQLLVAPTTVLRIDVAQRFEAAFPELAGQLAGQPAGQLELAGAPYLRRIVLTG
QTDRGWATRWDPRDPPLVRAELLAAVQTEVTPADLAIMVHTSGSTADPKGVLHTHGTLVRQTSTWPAAIRGLTGVDHAPR
ILCAMPFFWIGGILAATGALHAPVAVLVLARLEAGPALDLAERERANGVVGWPAFTQQLRLHPSFPSRDLRSAPALREGP
VDLAMAGVPDGHPIHRSLTESGGSFAFTETAIVDAAGERVPDGTVGELLIRGIGSMAGYNKRERAEVFDADGWYHTSDRV
YRRTGDPRLFYVGRDSELVKVAGSNVAPREVEAVIEEFPEVAHCVVTGVEHPTRGEEVCAVIVPAGTTGTDVDVDGLAAR
TRTLLSSYKVPTRWIVAADDEVPALPSGKPDRRGLRTLIEDGRLK

Sequences:

>Translated_525_residues
MTSTGDEIRPTIPDLLGRCAREFGSADYIVSLTDRLTYAEAEEQSARVARWLLHEGVGKGTRVGLFFPSGVEWALWWLAV
SRIGAVAVPLSTLYPPAEIAKVVRLADVQLLVAPTTVLRIDVAQRFEAAFPELAGQLAGQPAGQLELAGAPYLRRIVLTG
QTDRGWATRWDPRDPPLVRAELLAAVQTEVTPADLAIMVHTSGSTADPKGVLHTHGTLVRQTSTWPAAIRGLTGVDHAPR
ILCAMPFFWIGGILAATGALHAPVAVLVLARLEAGPALDLAERERANGVVGWPAFTQQLRLHPSFPSRDLRSAPALREGP
VDLAMAGVPDGHPIHRSLTESGGSFAFTETAIVDAAGERVPDGTVGELLIRGIGSMAGYNKRERAEVFDADGWYHTSDRV
YRRTGDPRLFYVGRDSELVKVAGSNVAPREVEAVIEEFPEVAHCVVTGVEHPTRGEEVCAVIVPAGTTGTDVDVDGLAAR
TRTLLSSYKVPTRWIVAADDEVPALPSGKPDRRGLRTLIEDGRLK
>Mature_524_residues
TSTGDEIRPTIPDLLGRCAREFGSADYIVSLTDRLTYAEAEEQSARVARWLLHEGVGKGTRVGLFFPSGVEWALWWLAVS
RIGAVAVPLSTLYPPAEIAKVVRLADVQLLVAPTTVLRIDVAQRFEAAFPELAGQLAGQPAGQLELAGAPYLRRIVLTGQ
TDRGWATRWDPRDPPLVRAELLAAVQTEVTPADLAIMVHTSGSTADPKGVLHTHGTLVRQTSTWPAAIRGLTGVDHAPRI
LCAMPFFWIGGILAATGALHAPVAVLVLARLEAGPALDLAERERANGVVGWPAFTQQLRLHPSFPSRDLRSAPALREGPV
DLAMAGVPDGHPIHRSLTESGGSFAFTETAIVDAAGERVPDGTVGELLIRGIGSMAGYNKRERAEVFDADGWYHTSDRVY
RRTGDPRLFYVGRDSELVKVAGSNVAPREVEAVIEEFPEVAHCVVTGVEHPTRGEEVCAVIVPAGTTGTDVDVDGLAART
RTLLSSYKVPTRWIVAADDEVPALPSGKPDRRGLRTLIEDGRLK

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=532, Percent_Identity=25.3759398496241, Blast_Score=125, Evalue=8e-29,
Organism=Escherichia coli, GI221142682, Length=527, Percent_Identity=25.8064516129032, Blast_Score=112, Evalue=8e-26,
Organism=Escherichia coli, GI145693145, Length=526, Percent_Identity=24.1444866920152, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1788107, Length=171, Percent_Identity=35.0877192982456, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1786810, Length=177, Percent_Identity=29.3785310734463, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71994703, Length=527, Percent_Identity=23.3396584440228, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI71994694, Length=527, Percent_Identity=23.3396584440228, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI71994690, Length=527, Percent_Identity=23.3396584440228, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17560308, Length=532, Percent_Identity=25.187969924812, Blast_Score=114, Evalue=9e-26,
Organism=Caenorhabditis elegans, GI17557194, Length=534, Percent_Identity=24.3445692883895, Blast_Score=102, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17538037, Length=538, Percent_Identity=24.907063197026, Blast_Score=99, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI32563687, Length=512, Percent_Identity=23.6328125, Blast_Score=97, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17559526, Length=359, Percent_Identity=25.3481894150418, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24581924, Length=543, Percent_Identity=24.6777163904236, Blast_Score=125, Evalue=8e-29,
Organism=Drosophila melanogaster, GI18859661, Length=541, Percent_Identity=26.0628465804067, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21356947, Length=384, Percent_Identity=26.8229166666667, Blast_Score=77, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 56455; Mature: 56324

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS00133 CARBOXYPEPT_ZN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSTGDEIRPTIPDLLGRCAREFGSADYIVSLTDRLTYAEAEEQSARVARWLLHEGVGKG
CCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCC
TRVGLFFPSGVEWALWWLAVSRIGAVAVPLSTLYPPAEIAKVVRLADVQLLVAPTTVLRI
CEEEEECCCCHHHHHHHHHHHHHCHHEECHHHCCCHHHHHHHHHHHCEEEEEECCEEEEE
DVAQRFEAAFPELAGQLAGQPAGQLELAGAPYLRRIVLTGQTDRGWATRWDPRDPPLVRA
HHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHH
ELLAAVQTEVTPADLAIMVHTSGSTADPKGVLHTHGTLVRQTSTWPAAIRGLTGVDHAPR
HHHHHHHHCCCCCEEEEEEECCCCCCCCCCEEECCCEEEEECCCCHHHHHCCCCCCCCCH
ILCAMPFFWIGGILAATGALHAPVAVLVLARLEAGPALDLAERERANGVVGWPAFTQQLR
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCEEECHHHHHHEE
LHPSFPSRDLRSAPALREGPVDLAMAGVPDGHPIHRSLTESGGSFAFTETAIVDAAGERV
ECCCCCCCHHHCCCCCCCCCCCEEEECCCCCCCHHHHHHCCCCCEEEEHHHHHHCCCCCC
PDGTVGELLIRGIGSMAGYNKRERAEVFDADGWYHTSDRVYRRTGDPRLFYVGRDSELVK
CCCHHHHHHHHHHHHHCCCCCHHHHHEECCCCCEECCCCCEEECCCCEEEEEECCCCEEE
VAGSNVAPREVEAVIEEFPEVAHCVVTGVEHPTRGEEVCAVIVPAGTTGTDVDVDGLAAR
ECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHH
TRTLLSSYKVPTRWIVAADDEVPALPSGKPDRRGLRTLIEDGRLK
HHHHHHHCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TSTGDEIRPTIPDLLGRCAREFGSADYIVSLTDRLTYAEAEEQSARVARWLLHEGVGKG
CCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCC
TRVGLFFPSGVEWALWWLAVSRIGAVAVPLSTLYPPAEIAKVVRLADVQLLVAPTTVLRI
CEEEEECCCCHHHHHHHHHHHHHCHHEECHHHCCCHHHHHHHHHHHCEEEEEECCEEEEE
DVAQRFEAAFPELAGQLAGQPAGQLELAGAPYLRRIVLTGQTDRGWATRWDPRDPPLVRA
HHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHH
ELLAAVQTEVTPADLAIMVHTSGSTADPKGVLHTHGTLVRQTSTWPAAIRGLTGVDHAPR
HHHHHHHHCCCCCEEEEEEECCCCCCCCCCEEECCCEEEEECCCCHHHHHCCCCCCCCCH
ILCAMPFFWIGGILAATGALHAPVAVLVLARLEAGPALDLAERERANGVVGWPAFTQQLR
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCEEECHHHHHHEE
LHPSFPSRDLRSAPALREGPVDLAMAGVPDGHPIHRSLTESGGSFAFTETAIVDAAGERV
ECCCCCCCHHHCCCCCCCCCCCEEEECCCCCCCHHHHHHCCCCCEEEEHHHHHHCCCCCC
PDGTVGELLIRGIGSMAGYNKRERAEVFDADGWYHTSDRVYRRTGDPRLFYVGRDSELVK
CCCHHHHHHHHHHHHHCCCCCHHHHHEECCCCCEECCCCCEEECCCCEEEEEECCCCEEE
VAGSNVAPREVEAVIEEFPEVAHCVVTGVEHPTRGEEVCAVIVPAGTTGTDVDVDGLAAR
ECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHH
TRTLLSSYKVPTRWIVAADDEVPALPSGKPDRRGLRTLIEDGRLK
HHHHHHHCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]