Click here to switch to the map view.

The map label for this gene is narX [H]

Identifier: 158317154

GI number: 158317154

Start: 6517219

End: 6518538

Strand: Reverse

Name: narX [H]

Synonym: Franean1_5401

Alternate gene names: 158317154

Gene position: 6518538-6517219 (Counterclockwise)

Preceding gene: 158317155

Following gene: 158317153

Centisome position: 72.57

GC content: 73.11

Gene sequence:

>1320_bases
ATGACCATCGAGGTGGTCGGGTTTGGGGGGCGCTGGGCCCGCGTGCGGGCGGCCGTCCAGGCGCGGGAGCCGTGGCCCCG
CGACCGGCGCGCGCTGGTGTTCGACCTCGTGGTCGCGCTAGCCGCCACGGTCGCCGAGCTCAGCCTCCTCCTCAACGACG
ACCACACTGTGCGTGCGCCGATGGTGCTGCTAGCGGTCGCGGCCGGCGGTGCGCTCACCGCGCGTCGCCGGGCACCGTGG
ACGGTGCTTGTGATGACGTTGGCCCTGTCCGGAGCGCTGGTGGCCATCGGTGACGCGCCCGGCGGGGTGCCCGTCCTGGT
GGCGCTCTACACGGTCGCCGACCTCGACGACTGGCGCCTCTCGCTCGCCGCGCTCGCGCCCACCGCGGTGCTCCTGACCA
TGTTGTCCATCGTCTCCGTCCCGCCGACGGCGGGGGTGTGGGCGCTGGGCGCCTACGCGCAGACCCGCCGCCGCTACGTC
CGCGCGCTCGAGGAACGCGCGGAGCACCTTCAACGCGAACGGGAGCAACTAGCCCGGATCGCGGTGCACGAGGAGCGGGC
GTCGATCGCCCGCGAGCTGCACGACATCGTCGCTCACTCGGTGACCGTGATGCTGCTGGGCGTGCGTGGCGCCCGCGACG
TGCTGCGCGTCTCCCCAGACCGAGCCGACGACACACTGGCGCGGGTGGAGACGAACGGGGAGCAGAGCCTCGTCGAGCTG
CGGCGGATACTGACCGTGCTGCGTGCTCCCGACACCCCCGCCGACTCACGTCCCGCCCCATCTCTGACGGAACTGGACGA
GCTCGTCGTCGACTACCGTGACGCCGGGCTGCCCATCCACCTACGGGTGACGGGGGAGCGAAGACCGCTTCCCGGCGGCG
TGGAGCTCTGCGTCTACCGCGTCATCCAGGAGGCGTTGACCAACGCGCTGAAGCACTCGCGCCCCAGGCATGTCACGGTC
ACGCTGGCCTTCCTGGGCTGGTGCCTCGACGTCGAGGTGGCCAACGACAGCACGGCCCCAGCGCCGGGGCCGACCGGCGA
TGGCGCCGGGGATATGCCCGAGACCGGGAACCCCGCTGGGCACGGCCTTATCGGGATGCGCGAGCGGGTTGGGGTGCTCG
GCGGCGAGCTGGAGTTCGGGCACCGACCCGGCGGCGGCTTCCGCGTCGCGGCCCGCCTGCCGCTGGGCGGCGGAGCTGCA
CGCCGCCAGCTACGCCGGCCGCGTCGACGTGGTCCGTCTGCTGCTCGACCGCGGCGCCGACATCGACGCCGCGGACCGGC
ATACGTGCCCAAGGGATCCGCGCGACCGTCGAGGAGATGA

Upstream 100 bases:

>100_bases
CGGGCCGCCTCCCGCTGGCCGCGGCGGCGACCGTTCTGATCATCTGGACGGTCGGCGTCATGGCCGCGGCGATGGCCGGC
GAACGCTGGCGTGACCTGGC

Downstream 100 bases:

>100_bases
CGGTCCACGGTGACACGATCACCCTCGGGCTCGCCGTCACCCGCGCCGGGCAGACCGAGCGCCCCGTCGGCCCGGCGGAT
CTGTTCTACCAGGTGTTCAC

Product: integral membrane sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 439; Mature: 438

Protein sequence:

>439_residues
MTIEVVGFGGRWARVRAAVQAREPWPRDRRALVFDLVVALAATVAELSLLLNDDHTVRAPMVLLAVAAGGALTARRRAPW
TVLVMTLALSGALVAIGDAPGGVPVLVALYTVADLDDWRLSLAALAPTAVLLTMLSIVSVPPTAGVWALGAYAQTRRRYV
RALEERAEHLQREREQLARIAVHEERASIARELHDIVAHSVTVMLLGVRGARDVLRVSPDRADDTLARVETNGEQSLVEL
RRILTVLRAPDTPADSRPAPSLTELDELVVDYRDAGLPIHLRVTGERRPLPGGVELCVYRVIQEALTNALKHSRPRHVTV
TLAFLGWCLDVEVANDSTAPAPGPTGDGAGDMPETGNPAGHGLIGMRERVGVLGGELEFGHRPGGGFRVAARLPLGGGAA
RRQLRRPRRRGPSAARPRRRHRRRGPAYVPKGSARPSRR

Sequences:

>Translated_439_residues
MTIEVVGFGGRWARVRAAVQAREPWPRDRRALVFDLVVALAATVAELSLLLNDDHTVRAPMVLLAVAAGGALTARRRAPW
TVLVMTLALSGALVAIGDAPGGVPVLVALYTVADLDDWRLSLAALAPTAVLLTMLSIVSVPPTAGVWALGAYAQTRRRYV
RALEERAEHLQREREQLARIAVHEERASIARELHDIVAHSVTVMLLGVRGARDVLRVSPDRADDTLARVETNGEQSLVEL
RRILTVLRAPDTPADSRPAPSLTELDELVVDYRDAGLPIHLRVTGERRPLPGGVELCVYRVIQEALTNALKHSRPRHVTV
TLAFLGWCLDVEVANDSTAPAPGPTGDGAGDMPETGNPAGHGLIGMRERVGVLGGELEFGHRPGGGFRVAARLPLGGGAA
RRQLRRPRRRGPSAARPRRRHRRRGPAYVPKGSARPSRR
>Mature_438_residues
TIEVVGFGGRWARVRAAVQAREPWPRDRRALVFDLVVALAATVAELSLLLNDDHTVRAPMVLLAVAAGGALTARRRAPWT
VLVMTLALSGALVAIGDAPGGVPVLVALYTVADLDDWRLSLAALAPTAVLLTMLSIVSVPPTAGVWALGAYAQTRRRYVR
ALEERAEHLQREREQLARIAVHEERASIARELHDIVAHSVTVMLLGVRGARDVLRVSPDRADDTLARVETNGEQSLVELR
RILTVLRAPDTPADSRPAPSLTELDELVVDYRDAGLPIHLRVTGERRPLPGGVELCVYRVIQEALTNALKHSRPRHVTVT
LAFLGWCLDVEVANDSTAPAPGPTGDGAGDMPETGNPAGHGLIGMRERVGVLGGELEFGHRPGGGFRVAARLPLGGGAAR
RQLRRPRRRGPSAARPRRRHRRRGPAYVPKGSARPSRR

Specific function: Acts as a sensor for nitrate/nitrite and transduces signal of nitrate availability to the narL protein and of both nitrate/nitrite to the narP protein. NarX probably activates narL and narP by phosphorylation in the presence of nitrate. NarX also plays a

COG id: COG4585

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787474, Length=222, Percent_Identity=30.6306306306306, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1788812, Length=231, Percent_Identity=28.1385281385281, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR016380
- InterPro:   IPR003661
- InterPro:   IPR011712
- InterPro:   IPR005467 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 47489; Mature: 47357

Theoretical pI: Translated: 11.51; Mature: 11.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIEVVGFGGRWARVRAAVQAREPWPRDRRALVFDLVVALAATVAELSLLLNDDHTVRAP
CEEEEEECCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
MVLLAVAAGGALTARRRAPWTVLVMTLALSGALVAIGDAPGGVPVLVALYTVADLDDWRL
HHHHHHHCCCCHHHCCCCCHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHCCCHHHH
SLAALAPTAVLLTMLSIVSVPPTAGVWALGAYAQTRRRYVRALEERAEHLQREREQLARI
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVHEERASIARELHDIVAHSVTVMLLGVRGARDVLRVSPDRADDTLARVETNGEQSLVEL
HHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHCCCCCCCHHHHHHHCCCHHHHHHH
RRILTVLRAPDTPADSRPAPSLTELDELVVDYRDAGLPIHLRVTGERRPLPGGVELCVYR
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHH
VIQEALTNALKHSRPRHVTVTLAFLGWCLDVEVANDSTAPAPGPTGDGAGDMPETGNPAG
HHHHHHHHHHHCCCCCEEEEEHHHHHHHEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCC
HGLIGMRERVGVLGGELEFGHRPGGGFRVAARLPLGGGAARRQLRRPRRRGPSAARPRRR
CCCCCHHHHHHHCCCCEECCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCHHHH
HRRRGPAYVPKGSARPSRR
HHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TIEVVGFGGRWARVRAAVQAREPWPRDRRALVFDLVVALAATVAELSLLLNDDHTVRAP
EEEEEECCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
MVLLAVAAGGALTARRRAPWTVLVMTLALSGALVAIGDAPGGVPVLVALYTVADLDDWRL
HHHHHHHCCCCHHHCCCCCHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHCCCHHHH
SLAALAPTAVLLTMLSIVSVPPTAGVWALGAYAQTRRRYVRALEERAEHLQREREQLARI
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVHEERASIARELHDIVAHSVTVMLLGVRGARDVLRVSPDRADDTLARVETNGEQSLVEL
HHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHCCCCCCCHHHHHHHCCCHHHHHHH
RRILTVLRAPDTPADSRPAPSLTELDELVVDYRDAGLPIHLRVTGERRPLPGGVELCVYR
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHH
VIQEALTNALKHSRPRHVTVTLAFLGWCLDVEVANDSTAPAPGPTGDGAGDMPETGNPAG
HHHHHHHHHHHCCCCCEEEEEHHHHHHHEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCC
HGLIGMRERVGVLGGELEFGHRPGGGFRVAARLPLGGGAARRQLRRPRRRGPSAARPRRR
CCCCCHHHHHHHCCCCEECCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCHHHH
HRRRGPAYVPKGSARPSRR
HHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]