Click here to switch to the map view.

The map label for this gene is proB [H]

Identifier: 158317013

GI number: 158317013

Start: 6319977

End: 6321092

Strand: Reverse

Name: proB [H]

Synonym: Franean1_5257

Alternate gene names: 158317013

Gene position: 6321092-6319977 (Counterclockwise)

Preceding gene: 158317014

Following gene: 158317012

Centisome position: 70.37

GC content: 75.27

Gene sequence:

>1116_bases
GTGGTCCGTTCCTTCGTCACCGAAGCGAACCGGGTCGTCGTCAAGGTCGGCTCGTCGTCGCTGACCACGGCGGCCGGTGG
GCTGGACGCCGACCGGGTCGAGGCGCTGGTCGCGGCCGTCGCCGGCCGGGCCGGCAGCCTGGTGCTGGTGTCGTCGGGCG
CGATCGCCGCCGGTCTCGCCCCGCTCGGGCTGACCAGGCGGCCGCGTGACCTGGCCACCCTGCAGGCGGCGGCGAGCGTC
GGCCAGAGCTCGCTCGTCCACTGCTACGCCGGCGCGTTCGGCCGGGCCGGGCACCGGGTCGGCCAGGTCCTGCTCACCGC
CACCGACGTGATCCGGCGGACGCACTACCGGAACGCGCGCACGACCCTGGAGCGGCTGCTGGCCCTCGGTGTCGTGCCGA
TCGTGAACGAGAACGACGCGGTCGCCACCCAGGAGATCCGGTTCGGTGACAACGACCGGCTCGCCGCGATCGTCTCCCAC
CTGGTCTCCGCAGACCTGCTCGTCCTGCTCTCCGACGTCGACGGCGTGTACGAGTCGAACCCGCGGCTGGGGCCGGCGAG
CATGGTGCGCGAGGTCCGCTCGGACGCCGATCTCGACGGCCTGACCGCCCGCGGCACCGGCGCGGCCGGCGTCGGGGTCG
GCGGCATGGCGACGAAGATCGACGCCGCGCGGATGGCGGCGTCCGGTGGCGTCACGACGGTCGTCACCTCTGCGGCCAAC
GCGGCCGCGGTGCTGGCCGGCGAGGAGATCGGGACGGTGTTCCACCCGACCGGCCCGCGCCGGCCGTCCCGCCTGCTCTG
GCTGGCCCACGCCACCGCTCCCGAGGGCCGGCTGCACCTGGACGAGGGCGCGGTCGCCGCGGTGGTGGACCGGCGGGCCT
CGCTGCTGCCGGCCGGGGTCACCAAGGTCGAGGGGGACTTCTCCGCCGGAGACCCGGTCGACCTGGTCGACCCGCGGGGC
CGTCCGGTGGCGCGCGGGCTGGTCGAGTTCGACTCCGTCGAGCTGCCGGGCATGCTCGGCCGCTCGACGATGGACCTGGC
GGCCGAGCGGGGCCCGTCCTACGAGCGTGAGGTCGTCCACCGCGACGACCTCGTCCTGCTCCTCACCCGTCGCTGA

Upstream 100 bases:

>100_bases
CGCTGACGACTTCGACGACGGTGCCGGCTTCAGCGACAGTGCGGCCTTCGGCGACAGCGGTGGCTCCGGCGGTGACGCCG
ACGGCGGGCGTGGCTAGGCC

Downstream 100 bases:

>100_bases
CCCGCCGCCGGTAGCCTCAGGGAACGGCGCACAGCGGCGCGCCGTCGACGCCGGGCCCGCGTCCGCGCCGGCCGTCGCGG
ACAGTCGGGAGGAATCCGGT

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MVRSFVTEANRVVVKVGSSSLTTAAGGLDADRVEALVAAVAGRAGSLVLVSSGAIAAGLAPLGLTRRPRDLATLQAAASV
GQSSLVHCYAGAFGRAGHRVGQVLLTATDVIRRTHYRNARTTLERLLALGVVPIVNENDAVATQEIRFGDNDRLAAIVSH
LVSADLLVLLSDVDGVYESNPRLGPASMVREVRSDADLDGLTARGTGAAGVGVGGMATKIDAARMAASGGVTTVVTSAAN
AAAVLAGEEIGTVFHPTGPRRPSRLLWLAHATAPEGRLHLDEGAVAAVVDRRASLLPAGVTKVEGDFSAGDPVDLVDPRG
RPVARGLVEFDSVELPGMLGRSTMDLAAERGPSYEREVVHRDDLVLLLTRR

Sequences:

>Translated_371_residues
MVRSFVTEANRVVVKVGSSSLTTAAGGLDADRVEALVAAVAGRAGSLVLVSSGAIAAGLAPLGLTRRPRDLATLQAAASV
GQSSLVHCYAGAFGRAGHRVGQVLLTATDVIRRTHYRNARTTLERLLALGVVPIVNENDAVATQEIRFGDNDRLAAIVSH
LVSADLLVLLSDVDGVYESNPRLGPASMVREVRSDADLDGLTARGTGAAGVGVGGMATKIDAARMAASGGVTTVVTSAAN
AAAVLAGEEIGTVFHPTGPRRPSRLLWLAHATAPEGRLHLDEGAVAAVVDRRASLLPAGVTKVEGDFSAGDPVDLVDPRG
RPVARGLVEFDSVELPGMLGRSTMDLAAERGPSYEREVVHRDDLVLLLTRR
>Mature_371_residues
MVRSFVTEANRVVVKVGSSSLTTAAGGLDADRVEALVAAVAGRAGSLVLVSSGAIAAGLAPLGLTRRPRDLATLQAAASV
GQSSLVHCYAGAFGRAGHRVGQVLLTATDVIRRTHYRNARTTLERLLALGVVPIVNENDAVATQEIRFGDNDRLAAIVSH
LVSADLLVLLSDVDGVYESNPRLGPASMVREVRSDADLDGLTARGTGAAGVGVGGMATKIDAARMAASGGVTTVVTSAAN
AAAVLAGEEIGTVFHPTGPRRPSRLLWLAHATAPEGRLHLDEGAVAAVVDRRASLLPAGVTKVEGDFSAGDPVDLVDPRG
RPVARGLVEFDSVELPGMLGRSTMDLAAERGPSYEREVVHRDDLVLLLTRR

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI21361368, Length=302, Percent_Identity=30.4635761589404, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI62912457, Length=300, Percent_Identity=30.6666666666667, Blast_Score=104, Evalue=1e-22,
Organism=Escherichia coli, GI1786437, Length=367, Percent_Identity=37.6021798365123, Blast_Score=221, Evalue=6e-59,
Organism=Caenorhabditis elegans, GI17569969, Length=343, Percent_Identity=32.9446064139942, Blast_Score=120, Evalue=9e-28,
Organism=Caenorhabditis elegans, GI17569967, Length=343, Percent_Identity=32.9446064139942, Blast_Score=120, Evalue=9e-28,
Organism=Saccharomyces cerevisiae, GI6320506, Length=419, Percent_Identity=31.5035799522673, Blast_Score=195, Evalue=8e-51,
Organism=Saccharomyces cerevisiae, GI6321822, Length=423, Percent_Identity=30.9692671394799, Blast_Score=180, Evalue=3e-46,
Organism=Drosophila melanogaster, GI21357643, Length=292, Percent_Identity=34.5890410958904, Blast_Score=127, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 38398; Mature: 38398

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: PS50890 PUA ; PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRSFVTEANRVVVKVGSSSLTTAAGGLDADRVEALVAAVAGRAGSLVLVSSGAIAAGLA
CCCHHHCCCCEEEEEECCCCCCHHCCCCCHHHHHHHHHHHHCCCCCEEEEECCCHHHCCC
PLGLTRRPRDLATLQAAASVGQSSLVHCYAGAFGRAGHRVGQVLLTATDVIRRTHYRNAR
CCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHH
TTLERLLALGVVPIVNENDAVATQEIRFGDNDRLAAIVSHLVSADLLVLLSDVDGVYESN
HHHHHHHHHCCEEEECCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCHHCCC
PRLGPASMVREVRSDADLDGLTARGTGAAGVGVGGMATKIDAARMAASGGVTTVVTSAAN
CCCCHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCC
AAAVLAGEEIGTVFHPTGPRRPSRLLWLAHATAPEGRLHLDEGAVAAVVDRRASLLPAGV
CEEEEECCCCCEEECCCCCCCCCEEEEEEECCCCCCCEEECCCCHHHHHHHHHHHCCCCC
TKVEGDFSAGDPVDLVDPRGRPVARGLVEFDSVELPGMLGRSTMDLAAERGPSYEREVVH
EEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCHHHHHHC
RDDLVLLLTRR
CCCEEEEEECC
>Mature Secondary Structure
MVRSFVTEANRVVVKVGSSSLTTAAGGLDADRVEALVAAVAGRAGSLVLVSSGAIAAGLA
CCCHHHCCCCEEEEEECCCCCCHHCCCCCHHHHHHHHHHHHCCCCCEEEEECCCHHHCCC
PLGLTRRPRDLATLQAAASVGQSSLVHCYAGAFGRAGHRVGQVLLTATDVIRRTHYRNAR
CCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHH
TTLERLLALGVVPIVNENDAVATQEIRFGDNDRLAAIVSHLVSADLLVLLSDVDGVYESN
HHHHHHHHHCCEEEECCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCHHCCC
PRLGPASMVREVRSDADLDGLTARGTGAAGVGVGGMATKIDAARMAASGGVTTVVTSAAN
CCCCHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCC
AAAVLAGEEIGTVFHPTGPRRPSRLLWLAHATAPEGRLHLDEGAVAAVVDRRASLLPAGV
CEEEEECCCCCEEECCCCCCCCCEEEEEEECCCCCCCEEECCCCHHHHHHHHHHHCCCCC
TKVEGDFSAGDPVDLVDPRGRPVARGLVEFDSVELPGMLGRSTMDLAAERGPSYEREVVH
EEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCHHHHHHC
RDDLVLLLTRR
CCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA