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The map label for this gene is metF [H]

Identifier: 158316882

GI number: 158316882

Start: 6140876

End: 6141751

Strand: Reverse

Name: metF [H]

Synonym: Franean1_5126

Alternate gene names: 158316882

Gene position: 6141751-6140876 (Counterclockwise)

Preceding gene: 158316883

Following gene: 158316881

Centisome position: 68.38

GC content: 69.41

Gene sequence:

>876_bases
ATGTCGACCCCAACGGTGGCCGAGTGCCTGGCCTCCGGACGTACCTCCTTCTCTTTCGAGTTCTTCCCACCGAAGACGAC
CGAGGGCGAGCGGCATCTGTGGACCGCGCTGCGTGAGATCGAGGCCCTGCACCCGACCTTCGTGTCGGTGACCTACGGAG
CCGGCGGTTCTACGCGGGACGGGACCATCCGGGTCACCGAGCGCATCGCCACCGAGACCACGCTGACGCCCATCGGGCAC
CTGACCGCGGTGAACCACTCGGTGGGCGAGCTGCGCCAGGTCATCGGCGGCTACGCCGGCGCGGGCGTGCGCAACGTGCT
GGCGCTGCGGGGCGATCCGCCGGGCGACCCGCAGGCGGAATGGGTGCGTCATCCCGACGGCCTGGGGCACGCCGACGAGC
TCGTGCGGCTCGTCCGTTCCCTCGGGGAGTTCTGCGTCGGCGTCGCCGCCTTCCCGGACAAACACCCGCGGTCGCCGGAT
CTGGAGACCGACGCGCGATTCCTGGTGCGAAAGCTGGACGCGGGCGCCGACTTCGCCATCACTCAGTTCTTCTTCGGTGC
CGACGACTATTTCCGGCTGGTCGAACGCGTACGGCGGCTCGGCTGTGACGCACCGATCATTCCGGGTGTGATGCCGGTTA
CCAATGTGGCCCAGATCGAGCGCATGGCGCAGCTTTCCGGCGCGGATTTCCCGGCGGATCTCGCGGCCCGTCTGCATGAG
GTCGCGGACGACCCCGCCGCGGTCCGGGCGATAGGTGTCGAGGTCGCCACGGAGCTCTCCGAGCGGCTGCTCGCCGGCGG
CGCGCCAGGGCTGCACTTCATCACGCTGAACCGTTCCAGCGCGACGCGTGAGATCTACCAGTCCCTGGGCCGCTGA

Upstream 100 bases:

>100_bases
CGGGCCCGGCAGGCCCTGTCCGGGGTGCGGGTCGCCGCCGGATTACTCTCTTCTGCATGACAGGGTCCCTCTCATGACGG
CGGCCGGGAGCACGACTCGG

Downstream 100 bases:

>100_bases
CGATCCGCCTGTGGCCTGTGGCCGCGCTCCCGTCCGGCCGGGGCGCGCGGCGGCGGGCGGCGTGGCGGTTCAGTCCTGTT
CCTCGTATGCGTCGAAGACG

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MSTPTVAECLASGRTSFSFEFFPPKTTEGERHLWTALREIEALHPTFVSVTYGAGGSTRDGTIRVTERIATETTLTPIGH
LTAVNHSVGELRQVIGGYAGAGVRNVLALRGDPPGDPQAEWVRHPDGLGHADELVRLVRSLGEFCVGVAAFPDKHPRSPD
LETDARFLVRKLDAGADFAITQFFFGADDYFRLVERVRRLGCDAPIIPGVMPVTNVAQIERMAQLSGADFPADLAARLHE
VADDPAAVRAIGVEVATELSERLLAGGAPGLHFITLNRSSATREIYQSLGR

Sequences:

>Translated_291_residues
MSTPTVAECLASGRTSFSFEFFPPKTTEGERHLWTALREIEALHPTFVSVTYGAGGSTRDGTIRVTERIATETTLTPIGH
LTAVNHSVGELRQVIGGYAGAGVRNVLALRGDPPGDPQAEWVRHPDGLGHADELVRLVRSLGEFCVGVAAFPDKHPRSPD
LETDARFLVRKLDAGADFAITQFFFGADDYFRLVERVRRLGCDAPIIPGVMPVTNVAQIERMAQLSGADFPADLAARLHE
VADDPAAVRAIGVEVATELSERLLAGGAPGLHFITLNRSSATREIYQSLGR
>Mature_290_residues
STPTVAECLASGRTSFSFEFFPPKTTEGERHLWTALREIEALHPTFVSVTYGAGGSTRDGTIRVTERIATETTLTPIGHL
TAVNHSVGELRQVIGGYAGAGVRNVLALRGDPPGDPQAEWVRHPDGLGHADELVRLVRSLGEFCVGVAAFPDKHPRSPDL
ETDARFLVRKLDAGADFAITQFFFGADDYFRLVERVRRLGCDAPIIPGVMPVTNVAQIERMAQLSGADFPADLAARLHEV
ADDPAAVRAIGVEVATELSERLLAGGAPGLHFITLNRSSATREIYQSLGR

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=288, Percent_Identity=36.1111111111111, Blast_Score=185, Evalue=4e-47,
Organism=Escherichia coli, GI1790377, Length=280, Percent_Identity=37.5, Blast_Score=202, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI25147436, Length=282, Percent_Identity=31.9148936170213, Blast_Score=152, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI25147441, Length=282, Percent_Identity=31.9148936170213, Blast_Score=152, Evalue=3e-37,
Organism=Saccharomyces cerevisiae, GI6321313, Length=289, Percent_Identity=34.6020761245675, Blast_Score=172, Evalue=7e-44,
Organism=Saccharomyces cerevisiae, GI6325234, Length=284, Percent_Identity=36.9718309859155, Blast_Score=167, Evalue=2e-42,
Organism=Drosophila melanogaster, GI24662658, Length=268, Percent_Identity=24.6268656716418, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 31396; Mature: 31265

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTPTVAECLASGRTSFSFEFFPPKTTEGERHLWTALREIEALHPTFVSVTYGAGGSTRD
CCCCHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCC
GTIRVTERIATETTLTPIGHLTAVNHSVGELRQVIGGYAGAGVRNVLALRGDPPGDPQAE
CEEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHEEECCCCCCCCCHH
WVRHPDGLGHADELVRLVRSLGEFCVGVAAFPDKHPRSPDLETDARFLVRKLDAGADFAI
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHH
TQFFFGADDYFRLVERVRRLGCDAPIIPGVMPVTNVAQIERMAQLSGADFPADLAARLHE
HHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHH
VADDPAAVRAIGVEVATELSERLLAGGAPGLHFITLNRSSATREIYQSLGR
HCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHCC
>Mature Secondary Structure 
STPTVAECLASGRTSFSFEFFPPKTTEGERHLWTALREIEALHPTFVSVTYGAGGSTRD
CCCHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCC
GTIRVTERIATETTLTPIGHLTAVNHSVGELRQVIGGYAGAGVRNVLALRGDPPGDPQAE
CEEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHEEECCCCCCCCCHH
WVRHPDGLGHADELVRLVRSLGEFCVGVAAFPDKHPRSPDLETDARFLVRKLDAGADFAI
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHH
TQFFFGADDYFRLVERVRRLGCDAPIIPGVMPVTNVAQIERMAQLSGADFPADLAARLHE
HHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHH
VADDPAAVRAIGVEVATELSERLLAGGAPGLHFITLNRSSATREIYQSLGR
HCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9515933 [H]