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The map label for this gene is murE [H]

Identifier: 158316860

GI number: 158316860

Start: 6108859

End: 6110400

Strand: Reverse

Name: murE [H]

Synonym: Franean1_5103

Alternate gene names: 158316860

Gene position: 6110400-6108859 (Counterclockwise)

Preceding gene: 158316861

Following gene: 158316859

Centisome position: 68.03

GC content: 76.98

Gene sequence:

>1542_bases
GTGACCACCCCGGCACTACGTCCGGCAGCGCCGTCACCGCGGTCGCTCACCGCTCTGCCCGACGTCCTCAGATCCATCAC
CGTCGCGCCCGGTCCGTCGGGCGGGGCGGCCCTCGCCGGTGTCGTCCTCACCGGCGTCACCCACGACTCGCGCGCGGTGC
TGCCGGGCGACCTCTACGCGGCGCTGCCCGGCGCGCACGTGCACGGTGCCGACTTCGCGGCCGGCGCGGTCGCCGCCGGC
GCCGTCGCCGTCCTCACCGACCCGGTGGGCGCCGCGGCGCTGTCCGGCCGGCTCGCGGTGCCCGTGCTCGTCACCGACGA
CCCGCGCCGCGACCTGGCGCCGGCCGCCGCCTGGATCTACGGCGACCCGTCGAGCGCGCTGACCCTGCTCGGGGTCACCG
GCACGAACGGCAAGACCACCACCGCCTTCCTCCTGGACGCCGGCCTGCGCGCCGCCGGGTACACCACGGGCCTGCTCGGC
ACGGTGCAGACCCGGGTCGCCGGAACGGTGGTGCCCTCGGTGCGCACGACGCCCGAGTCCGGCGACCTGCAGGCGCTGCT
CGCGACGATGGTGGAGCGGGGCGTCGGGGCGGCGTCGATGGAGGTGTCGAGCCACGCGCTGGCCCAGCACCGGGTGGACG
CGCTGCGCTTCGCCGGCGCCGCCTTCACCAACCTCAGCCAGGACCACCTCGACTTCCACCCGACGATGGAGGACTACTTC
GCGGCGAAGGCGGCACTGTTCGAGCCCGCCCGCAGCGCGGCCGCCGTGGTCTGCGTCGACGACGACTGGGGCCGCCGGCT
CGCGGCGCTGCGCCCGGACGCGCGCACCTACTCGGTGACCGGCCGCCCGGCCGACTGGTGGCCGGAGGACGTCGTCGCCG
GGCCGGGCGGCAGCGTGTTCCGCGCGCTGGGCCCGGACGGCGCGAAGGCGGACCTCTCGCTCGCCCTGCCGGGCCGGTTC
AACGTCGCCAACGCGCTGGGCGCCCTCGCGCTGCTGGCCGCTGTCGGTGTTCCGCTGGAGGCGGCGGCCGAGGGCATCTC
GTCGCTGCCGGGGGTCCCGGGCCGGATGGAGCGGATCGACGCCGGCCAGCCGTTCCTCGCCCTCGTCGACTACGCCCACA
CCCCCGGCGCGGTCGAGACGCTGCTCGCCACCGTGCGCCCGATCGTGACCGGGCGGGTGGTCGTCGTCCTCGGCTGCGGT
GGGGACCGCGACCGCGCCAAACGGCCGCTGATGGGCGCGGCCGCGGCGCGGCTGGCCGACCTGGCGGTGTTCACCAGTGA
CAACCCCCGGTCCGAGGACCCGGCGCGCATCCTCGAGCAGATGCTGGCCGGTGCCCGCGGCGCCCCCGGAGCGGGCGAGA
TCGTTGTGGAGCCGGACCGGGCGAGCGCGATCGCGCTCGCGGTCGGCGCCGCCGGGCCCACCGACGCCGTGATCGTGGCC
GGAAAGGGGCACGAGAGCGGGCAGGACGTCGCCGGTGTCGTCACGCCGTTCGACGACCGCGAGGTGCTGCGCTCCGCGCT
GCTGGCGGCGGCGAGCCGATGA

Upstream 100 bases:

>100_bases
GCCCAGGCTGTCCCGACACCTGCGCGTCAGCCCGGTGGACCACCGCCGTCCACGGCGCCCGTGCGGATCCCCCGCCTGCC
CGCCCACTAAGGTCGTCCCC

Downstream 100 bases:

>100_bases
TCGCGCTGACGCTCGCCGAGGTGGCCGCCGCCACCGGTGGCCGGCTCACCGCCGGCGCGGACCGGTTGGCCCCGGTCACG
TCGGTGGTGATCGACTCCCG

Product: UDP-N-acetylmuramyl-tripeptide synthetase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 513; Mature: 512

Protein sequence:

>513_residues
MTTPALRPAAPSPRSLTALPDVLRSITVAPGPSGGAALAGVVLTGVTHDSRAVLPGDLYAALPGAHVHGADFAAGAVAAG
AVAVLTDPVGAAALSGRLAVPVLVTDDPRRDLAPAAAWIYGDPSSALTLLGVTGTNGKTTTAFLLDAGLRAAGYTTGLLG
TVQTRVAGTVVPSVRTTPESGDLQALLATMVERGVGAASMEVSSHALAQHRVDALRFAGAAFTNLSQDHLDFHPTMEDYF
AAKAALFEPARSAAAVVCVDDDWGRRLAALRPDARTYSVTGRPADWWPEDVVAGPGGSVFRALGPDGAKADLSLALPGRF
NVANALGALALLAAVGVPLEAAAEGISSLPGVPGRMERIDAGQPFLALVDYAHTPGAVETLLATVRPIVTGRVVVVLGCG
GDRDRAKRPLMGAAAARLADLAVFTSDNPRSEDPARILEQMLAGARGAPGAGEIVVEPDRASAIALAVGAAGPTDAVIVA
GKGHESGQDVAGVVTPFDDREVLRSALLAAASR

Sequences:

>Translated_513_residues
MTTPALRPAAPSPRSLTALPDVLRSITVAPGPSGGAALAGVVLTGVTHDSRAVLPGDLYAALPGAHVHGADFAAGAVAAG
AVAVLTDPVGAAALSGRLAVPVLVTDDPRRDLAPAAAWIYGDPSSALTLLGVTGTNGKTTTAFLLDAGLRAAGYTTGLLG
TVQTRVAGTVVPSVRTTPESGDLQALLATMVERGVGAASMEVSSHALAQHRVDALRFAGAAFTNLSQDHLDFHPTMEDYF
AAKAALFEPARSAAAVVCVDDDWGRRLAALRPDARTYSVTGRPADWWPEDVVAGPGGSVFRALGPDGAKADLSLALPGRF
NVANALGALALLAAVGVPLEAAAEGISSLPGVPGRMERIDAGQPFLALVDYAHTPGAVETLLATVRPIVTGRVVVVLGCG
GDRDRAKRPLMGAAAARLADLAVFTSDNPRSEDPARILEQMLAGARGAPGAGEIVVEPDRASAIALAVGAAGPTDAVIVA
GKGHESGQDVAGVVTPFDDREVLRSALLAAASR
>Mature_512_residues
TTPALRPAAPSPRSLTALPDVLRSITVAPGPSGGAALAGVVLTGVTHDSRAVLPGDLYAALPGAHVHGADFAAGAVAAGA
VAVLTDPVGAAALSGRLAVPVLVTDDPRRDLAPAAAWIYGDPSSALTLLGVTGTNGKTTTAFLLDAGLRAAGYTTGLLGT
VQTRVAGTVVPSVRTTPESGDLQALLATMVERGVGAASMEVSSHALAQHRVDALRFAGAAFTNLSQDHLDFHPTMEDYFA
AKAALFEPARSAAAVVCVDDDWGRRLAALRPDARTYSVTGRPADWWPEDVVAGPGGSVFRALGPDGAKADLSLALPGRFN
VANALGALALLAAVGVPLEAAAEGISSLPGVPGRMERIDAGQPFLALVDYAHTPGAVETLLATVRPIVTGRVVVVLGCGG
DRDRAKRPLMGAAAARLADLAVFTSDNPRSEDPARILEQMLAGARGAPGAGEIVVEPDRASAIALAVGAAGPTDAVIVAG
KGHESGQDVAGVVTPFDDREVLRSALLAAASR

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=468, Percent_Identity=40.5982905982906, Blast_Score=257, Evalue=2e-69,
Organism=Escherichia coli, GI1786274, Length=401, Percent_Identity=27.6807980049875, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 51741; Mature: 51610

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTPALRPAAPSPRSLTALPDVLRSITVAPGPSGGAALAGVVLTGVTHDSRAVLPGDLYA
CCCCCCCCCCCCCCCHHHHHHHHHHEECCCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHH
ALPGAHVHGADFAAGAVAAGAVAVLTDPVGAAALSGRLAVPVLVTDDPRRDLAPAAAWIY
HCCCCCCCCCCHHHHHHHHCEEEEEECCCCHHHHCCCEEEEEEECCCCCCCCCCCEEEEE
GDPSSALTLLGVTGTNGKTTTAFLLDAGLRAAGYTTGLLGTVQTRVAGTVVPSVRTTPES
CCCCCCEEEEEEECCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHEECCCCCCCCCC
GDLQALLATMVERGVGAASMEVSSHALAQHRVDALRFAGAAFTNLSQDHLDFHPTMEDYF
CCHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCHHHHH
AAKAALFEPARSAAAVVCVDDDWGRRLAALRPDARTYSVTGRPADWWPEDVVAGPGGSVF
HHHHHHHCCCCCCEEEEEECCCHHCHHHHCCCCCCEEEECCCCCCCCCHHHCCCCCCCEE
RALGPDGAKADLSLALPGRFNVANALGALALLAAVGVPLEAAAEGISSLPGVPGRMERID
EECCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHCC
AGQPFLALVDYAHTPGAVETLLATVRPIVTGRVVVVLGCGGDRDRAKRPLMGAAAARLAD
CCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHCCHHHHHHHHHHH
LAVFTSDNPRSEDPARILEQMLAGARGAPGAGEIVVEPDRASAIALAVGAAGPTDAVIVA
EEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCEEECCCCCCEEEEEECCCCCCCEEEEE
GKGHESGQDVAGVVTPFDDREVLRSALLAAASR
ECCCCCCCCCEEEECCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TTPALRPAAPSPRSLTALPDVLRSITVAPGPSGGAALAGVVLTGVTHDSRAVLPGDLYA
CCCCCCCCCCCCCCHHHHHHHHHHEECCCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHH
ALPGAHVHGADFAAGAVAAGAVAVLTDPVGAAALSGRLAVPVLVTDDPRRDLAPAAAWIY
HCCCCCCCCCCHHHHHHHHCEEEEEECCCCHHHHCCCEEEEEEECCCCCCCCCCCEEEEE
GDPSSALTLLGVTGTNGKTTTAFLLDAGLRAAGYTTGLLGTVQTRVAGTVVPSVRTTPES
CCCCCCEEEEEEECCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHEECCCCCCCCCC
GDLQALLATMVERGVGAASMEVSSHALAQHRVDALRFAGAAFTNLSQDHLDFHPTMEDYF
CCHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCHHHHH
AAKAALFEPARSAAAVVCVDDDWGRRLAALRPDARTYSVTGRPADWWPEDVVAGPGGSVF
HHHHHHHCCCCCCEEEEEECCCHHCHHHHCCCCCCEEEECCCCCCCCCHHHCCCCCCCEE
RALGPDGAKADLSLALPGRFNVANALGALALLAAVGVPLEAAAEGISSLPGVPGRMERID
EECCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHCC
AGQPFLALVDYAHTPGAVETLLATVRPIVTGRVVVVLGCGGDRDRAKRPLMGAAAARLAD
CCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHCCHHHHHHHHHHH
LAVFTSDNPRSEDPARILEQMLAGARGAPGAGEIVVEPDRASAIALAVGAAGPTDAVIVA
EEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCEEECCCCCCEEEEEECCCCCCCEEEEE
GKGHESGQDVAGVVTPFDDREVLRSALLAAASR
ECCCCCCCCCEEEECCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12000953 [H]