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The map label for this gene is murF [H]

Identifier: 158316859

GI number: 158316859

Start: 6107384

End: 6108862

Strand: Reverse

Name: murF [H]

Synonym: Franean1_5102

Alternate gene names: 158316859

Gene position: 6108862-6107384 (Counterclockwise)

Preceding gene: 158316860

Following gene: 158316858

Centisome position: 68.01

GC content: 77.96

Gene sequence:

>1479_bases
ATGATCGCGCTGACGCTCGCCGAGGTGGCCGCCGCCACCGGTGGCCGGCTCACCGCCGGCGCGGACCGGTTGGCCCCGGT
CACGTCGGTGGTGATCGACTCCCGTCAGGTCGGCCCGGGCGCGCTGTTCGTCGCCCTGCCCGGTGAGCGGGTGGACGGGC
ACGACTTCGCCGCCGGCGCGGTCGCGGCCGGCGCGGTGGCCGTCCTCGGCGCCCGGGAGACGGGCGTGCCCACCGTCGTC
GCCGACCCGGCGGCGGCGCTGGCCGCGCTCGCCGCGCACCTGCGCGACCTCGCCCCGGCGACCGTGGTCGGGGTGACCGG
CTCGGCCGGTAAGACGACCACCAAGGACCTGCTGGCCGACCTGCTCGGCCGGCTCGGCCCGACGGTCGCCGCGGTGGGCT
CGTTCAACAACGAGATCGGCCTGCCGCTGACCCTGCTGCGCACCGAGCCCGACACCGCGTTCGTGGTGCTGGAGATGGGC
GCCCGCGGCATCGGGCACATCGCGACGCTGTGCGCGGTGGCCCGCCCGCACGTCGGGCTGGTACTCAACGTCGGGTCGGC
GCACGTGGGGGAGTACGCCGACGGGCGGAACGGCATCGCCGCGGCGAAGGGCGAGCTCGCAGAGGCGGCCACCGGCCTGG
TCGTGCTGAACGCCGACGATCCGCTGGTGGCGGCGATGGCCGGCCGCGCCGGCGCCCAGGTGGTCACCTTCGGCCGCGCC
GCGGCGGCGGACGTCCGCGCCGAGCGCGTCGAGGTGGGCGGTGACGGCCGGGCCGCGTTCGACCTGCTGGCCGGCGGGGA
GCGCCACCGGCTGCGCCTCGGCCTCGTCGGCGCGCACCAGGTGGCCAACGCGCTGGCCGCCGCGGCGGTGGCGACATCAC
TCGGCCTCGCGCCGGCCGAGGCCGCGGCGGCGCTCGAGGAGGCCCGCCCGCGCAGCCGCTGGCGGATGGAGGTGACGACC
ACCCCGGACGGCGTGGTCGTGCTCAACGACTCCTACAACGCGAACCCCGAGTCGATGCGCGCGGCCCTGACCGCGCTGAT
GGACATGCGGGGAGCGGGGCGGGCGTGGGCGGTGCTGGGCCCGATGGGCGAGCTCGGCGCCGACGCGGACGCCGCGCACG
ACACCCTGGGGCGGCTCGCCGCCGGGCTGGGCGTGTCACGGCTGGTGGCGGTCGGCGCCGGGGCGCGCCGGGTGCATCAG
GCGGCGGAGCACGAGGGCGCCTGGGCCGGCGAGTCGACCTGGGTGCCCGGGGTGGACGAGGCGGTCGCGCTGCTGGCCGC
GCAGGTCCGCCCGGGTGACGTGGTTCTGGTGAAGGCGAGCCGGTCGTTCGGGCTCGAGCGGGTGGCCACGGGGCTGACCG
CGGATGGTGGGGCCGGCCCAGCGGGCCGGCGGGACGGCGCCGCCGGCGCCGCGATCGAGGGGATCGAGAACACGTGGAAC
ACGGTGGGACACTCCGGCGAACCAGGCGTGCGCGGGTGA

Upstream 100 bases:

>100_bases
CCGGAAAGGGGCACGAGAGCGGGCAGGACGTCGCCGGTGTCGTCACGCCGTTCGACGACCGCGAGGTGCTGCGCTCCGCG
CTGCTGGCGGCGGCGAGCCG

Downstream 100 bases:

>100_bases
CGCTCGCGTGAGAGGAGTTCTCTTCGCGGCACTGGTCGCGCTGGTCATCTCCCTGCTCGGCACCCCGTCGGTGATCCGCT
TCTTCCGGCGGCAGGGCTAC

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate--D-alanyl-D-alanyl ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 492; Mature: 492

Protein sequence:

>492_residues
MIALTLAEVAAATGGRLTAGADRLAPVTSVVIDSRQVGPGALFVALPGERVDGHDFAAGAVAAGAVAVLGARETGVPTVV
ADPAAALAALAAHLRDLAPATVVGVTGSAGKTTTKDLLADLLGRLGPTVAAVGSFNNEIGLPLTLLRTEPDTAFVVLEMG
ARGIGHIATLCAVARPHVGLVLNVGSAHVGEYADGRNGIAAAKGELAEAATGLVVLNADDPLVAAMAGRAGAQVVTFGRA
AAADVRAERVEVGGDGRAAFDLLAGGERHRLRLGLVGAHQVANALAAAAVATSLGLAPAEAAAALEEARPRSRWRMEVTT
TPDGVVVLNDSYNANPESMRAALTALMDMRGAGRAWAVLGPMGELGADADAAHDTLGRLAAGLGVSRLVAVGAGARRVHQ
AAEHEGAWAGESTWVPGVDEAVALLAAQVRPGDVVLVKASRSFGLERVATGLTADGGAGPAGRRDGAAGAAIEGIENTWN
TVGHSGEPGVRG

Sequences:

>Translated_492_residues
MIALTLAEVAAATGGRLTAGADRLAPVTSVVIDSRQVGPGALFVALPGERVDGHDFAAGAVAAGAVAVLGARETGVPTVV
ADPAAALAALAAHLRDLAPATVVGVTGSAGKTTTKDLLADLLGRLGPTVAAVGSFNNEIGLPLTLLRTEPDTAFVVLEMG
ARGIGHIATLCAVARPHVGLVLNVGSAHVGEYADGRNGIAAAKGELAEAATGLVVLNADDPLVAAMAGRAGAQVVTFGRA
AAADVRAERVEVGGDGRAAFDLLAGGERHRLRLGLVGAHQVANALAAAAVATSLGLAPAEAAAALEEARPRSRWRMEVTT
TPDGVVVLNDSYNANPESMRAALTALMDMRGAGRAWAVLGPMGELGADADAAHDTLGRLAAGLGVSRLVAVGAGARRVHQ
AAEHEGAWAGESTWVPGVDEAVALLAAQVRPGDVVLVKASRSFGLERVATGLTADGGAGPAGRRDGAAGAAIEGIENTWN
TVGHSGEPGVRG
>Mature_492_residues
MIALTLAEVAAATGGRLTAGADRLAPVTSVVIDSRQVGPGALFVALPGERVDGHDFAAGAVAAGAVAVLGARETGVPTVV
ADPAAALAALAAHLRDLAPATVVGVTGSAGKTTTKDLLADLLGRLGPTVAAVGSFNNEIGLPLTLLRTEPDTAFVVLEMG
ARGIGHIATLCAVARPHVGLVLNVGSAHVGEYADGRNGIAAAKGELAEAATGLVVLNADDPLVAAMAGRAGAQVVTFGRA
AAADVRAERVEVGGDGRAAFDLLAGGERHRLRLGLVGAHQVANALAAAAVATSLGLAPAEAAAALEEARPRSRWRMEVTT
TPDGVVVLNDSYNANPESMRAALTALMDMRGAGRAWAVLGPMGELGADADAAHDTLGRLAAGLGVSRLVAVGAGARRVHQ
AAEHEGAWAGESTWVPGVDEAVALLAAQVRPGDVVLVKASRSFGLERVATGLTADGGAGPAGRRDGAAGAAIEGIENTWN
TVGHSGEPGVRG

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=468, Percent_Identity=35.8974358974359, Blast_Score=184, Evalue=8e-48,
Organism=Escherichia coli, GI1786276, Length=345, Percent_Identity=31.0144927536232, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 48883; Mature: 48883

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIALTLAEVAAATGGRLTAGADRLAPVTSVVIDSRQVGPGALFVALPGERVDGHDFAAGA
CEEEEEHHHHHCCCCCEECCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCHHHH
VAAGAVAVLGARETGVPTVVADPAAALAALAAHLRDLAPATVVGVTGSAGKTTTKDLLAD
HHHHHHHEEECCCCCCCEEEECHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHH
LLGRLGPTVAAVGSFNNEIGLPLTLLRTEPDTAFVVLEMGARGIGHIATLCAVARPHVGL
HHHHCCCCEEECCCCCCCCCCCEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHCCCCEE
VLNVGSAHVGEYADGRNGIAAAKGELAEAATGLVVLNADDPLVAAMAGRAGAQVVTFGRA
EEECCCCCCCCCCCCCCCCCCCCCHHHHHHCCEEEECCCCCEEHHHHCCCCCEEEEECCH
AAADVRAERVEVGGDGRAAFDLLAGGERHRLRLGLVGAHQVANALAAAAVATSLGLAPAE
HHHHHHHHEEEECCCCCHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCHHH
AAAALEEARPRSRWRMEVTTTPDGVVVLNDSYNANPESMRAALTALMDMRGAGRAWAVLG
HHHHHHHCCCCCCEEEEEEECCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEC
PMGELGADADAAHDTLGRLAAGLGVSRLVAVGAGARRVHQAAEHEGAWAGESTWVPGVDE
CCHHCCCCCHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCHHH
AVALLAAQVRPGDVVLVKASRSFGLERVATGLTADGGAGPAGRRDGAAGAAIEGIENTWN
HHHHHHHCCCCCCEEEEECCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
TVGHSGEPGVRG
HCCCCCCCCCCC
>Mature Secondary Structure
MIALTLAEVAAATGGRLTAGADRLAPVTSVVIDSRQVGPGALFVALPGERVDGHDFAAGA
CEEEEEHHHHHCCCCCEECCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCHHHH
VAAGAVAVLGARETGVPTVVADPAAALAALAAHLRDLAPATVVGVTGSAGKTTTKDLLAD
HHHHHHHEEECCCCCCCEEEECHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHH
LLGRLGPTVAAVGSFNNEIGLPLTLLRTEPDTAFVVLEMGARGIGHIATLCAVARPHVGL
HHHHCCCCEEECCCCCCCCCCCEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHCCCCEE
VLNVGSAHVGEYADGRNGIAAAKGELAEAATGLVVLNADDPLVAAMAGRAGAQVVTFGRA
EEECCCCCCCCCCCCCCCCCCCCCHHHHHHCCEEEECCCCCEEHHHHCCCCCEEEEECCH
AAADVRAERVEVGGDGRAAFDLLAGGERHRLRLGLVGAHQVANALAAAAVATSLGLAPAE
HHHHHHHHEEEECCCCCHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCHHH
AAAALEEARPRSRWRMEVTTTPDGVVVLNDSYNANPESMRAALTALMDMRGAGRAWAVLG
HHHHHHHCCCCCCEEEEEEECCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEC
PMGELGADADAAHDTLGRLAAGLGVSRLVAVGAGARRVHQAAEHEGAWAGESTWVPGVDE
CCHHCCCCCHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCHHH
AVALLAAQVRPGDVVLVKASRSFGLERVATGLTADGGAGPAGRRDGAAGAAIEGIENTWN
HHHHHHHCCCCCCEEEEECCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
TVGHSGEPGVRG
HCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]