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The map label for this gene is dnaE [H]

Identifier: 158316805

GI number: 158316805

Start: 6047350

End: 6048132

Strand: Reverse

Name: dnaE [H]

Synonym: Franean1_5047

Alternate gene names: 158316805

Gene position: 6048132-6047350 (Counterclockwise)

Preceding gene: 158316806

Following gene: 158316804

Centisome position: 67.34

GC content: 71.01

Gene sequence:

>783_bases
GTGACGGACTCCCGCGCCGCACTCGACGAGCTCGCCGCCCTCTCGGTGGGGGCAGGTAGCCCGCAGGAAGGCCACGAGCC
GACCCACTATTTCTCCCACCGCTTGTGGCTGCCCGGCATCGCGACGACCAACGGCCAGGCCCGGTGGGGCGCTCTGGGGC
TCGGCCGCACGCCGGAGGGGGCCGCCGACCAGCTCCAGGGCGATCTGACCAGCCTGCCCGAGGGGCAGTGGGGCGAGGCC
GCCCAGTTCGACCAGGGCCGGAATGGGCCGAGGCGCAGCGACTGGCCGACCCGGCGCGTCGCCTGGGACGGCGAGCGGTG
GGAGTACGACGACGAGGCGCTGGCCGCGAAATCCCACATCGAGTACGCCGACCGCAAGAACGGCCGCAAGCCGGTCGTGC
CGGTCCATCCCGAGCTGGATGGACCGTTGGAGGACATCCTCGGCGCGACGTACCACCTGGTGGTCTACCAGGAGCAGGTG
ATGACCATCGCCCGGGAGCTGGCCGGGTACAGCCTGGGCCGCGCGGACCTGCTGCGCCGGGCGATGGGCAAGAAGAAGAA
GGACGTGCTGGACGCTCACCACGCGGAGTTCACCGCCGGCATGACCGGGCGGGGCTACTCGGCCGAGGCCGCGCAGGCCC
TGTGGGACGTGCTGGTGCCGTTCTCCGGCTACAGGTTCAACAAGTCCCACACCGCCGGCTACGGCGTGGTGGCGTTCTGG
ACCGCCTACCTGAAGGCCAACTTCCCGGCCGAGTACATGGCCGCACTGCTGACCTCAGTGTGA

Upstream 100 bases:

>100_bases
CCTACGACCACGGAGAGATCGCCCGGCTGACCGGGCAGCCCACGTCAGGTGCGAGCCCGTTCCTGCCGTCGATGAGCCCG
CGGGCGCGGCGGGCGGAGAC

Downstream 100 bases:

>100_bases
ACCGCCCCGGGTCAGGGCGGCGTTTTGCCATGGTTGTTCATCATGGCTGAAGGCGGTCGACTGCGTCGGCAACAGTTGCG
TGATCGACGGGCCTGCCCAG

Product: DNA-directed DNA polymerase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MTDSRAALDELAALSVGAGSPQEGHEPTHYFSHRLWLPGIATTNGQARWGALGLGRTPEGAADQLQGDLTSLPEGQWGEA
AQFDQGRNGPRRSDWPTRRVAWDGERWEYDDEALAAKSHIEYADRKNGRKPVVPVHPELDGPLEDILGATYHLVVYQEQV
MTIARELAGYSLGRADLLRRAMGKKKKDVLDAHHAEFTAGMTGRGYSAEAAQALWDVLVPFSGYRFNKSHTAGYGVVAFW
TAYLKANFPAEYMAALLTSV

Sequences:

>Translated_260_residues
MTDSRAALDELAALSVGAGSPQEGHEPTHYFSHRLWLPGIATTNGQARWGALGLGRTPEGAADQLQGDLTSLPEGQWGEA
AQFDQGRNGPRRSDWPTRRVAWDGERWEYDDEALAAKSHIEYADRKNGRKPVVPVHPELDGPLEDILGATYHLVVYQEQV
MTIARELAGYSLGRADLLRRAMGKKKKDVLDAHHAEFTAGMTGRGYSAEAAQALWDVLVPFSGYRFNKSHTAGYGVVAFW
TAYLKANFPAEYMAALLTSV
>Mature_259_residues
TDSRAALDELAALSVGAGSPQEGHEPTHYFSHRLWLPGIATTNGQARWGALGLGRTPEGAADQLQGDLTSLPEGQWGEAA
QFDQGRNGPRRSDWPTRRVAWDGERWEYDDEALAAKSHIEYADRKNGRKPVVPVHPELDGPLEDILGATYHLVVYQEQVM
TIARELAGYSLGRADLLRRAMGKKKKDVLDAHHAEFTAGMTGRGYSAEAAQALWDVLVPFSGYRFNKSHTAGYGVVAFWT
AYLKANFPAEYMAALLTSV

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The alpha chain is the DNA polymerase [H]

COG id: COG0587

COG function: function code L; DNA polymerase III, alpha subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA polymerase type-C family. DnaE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786381, Length=141, Percent_Identity=44.6808510638298, Blast_Score=126, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011708
- InterPro:   IPR004365
- InterPro:   IPR004013
- InterPro:   IPR003141
- InterPro:   IPR016195
- InterPro:   IPR004805 [H]

Pfam domain/function: PF07733 DNA_pol3_alpha; PF02811 PHP; PF01336 tRNA_anti [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 28568; Mature: 28437

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDSRAALDELAALSVGAGSPQEGHEPTHYFSHRLWLPGIATTNGQARWGALGLGRTPEG
CCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCEEECCCEECCCCCCCCCCCCCCCCCCC
AADQLQGDLTSLPEGQWGEAAQFDQGRNGPRRSDWPTRRVAWDGERWEYDDEALAAKSHI
HHHHHHCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHH
EYADRKNGRKPVVPVHPELDGPLEDILGATYHLVVYQEQVMTIARELAGYSLGRADLLRR
HHHHHCCCCCCEEECCCCCCCCHHHHHCCCEEEEHHHHHHHHHHHHHHCCCCCHHHHHHH
AMGKKKKDVLDAHHAEFTAGMTGRGYSAEAAQALWDVLVPFSGYRFNKSHTAGYGVVAFW
HHCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCEECCCCCCCCHHHHHH
TAYLKANFPAEYMAALLTSV
HHHHHCCCCHHHHHHHHHCC
>Mature Secondary Structure 
TDSRAALDELAALSVGAGSPQEGHEPTHYFSHRLWLPGIATTNGQARWGALGLGRTPEG
CCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCEEECCCEECCCCCCCCCCCCCCCCCCC
AADQLQGDLTSLPEGQWGEAAQFDQGRNGPRRSDWPTRRVAWDGERWEYDDEALAAKSHI
HHHHHHCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHH
EYADRKNGRKPVVPVHPELDGPLEDILGATYHLVVYQEQVMTIARELAGYSLGRADLLRR
HHHHHCCCCCCEEECCCCCCCCHHHHHCCCEEEEHHHHHHHHHHHHHHCCCCCHHHHHHH
AMGKKKKDVLDAHHAEFTAGMTGRGYSAEAAQALWDVLVPFSGYRFNKSHTAGYGVVAFW
HHCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCEECCCCCCCCHHHHHH
TAYLKANFPAEYMAALLTSV
HHHHHCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]