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The map label for this gene is dop [H]

Identifier: 158316644

GI number: 158316644

Start: 5854500

End: 5856224

Strand: Reverse

Name: dop [H]

Synonym: Franean1_4881

Alternate gene names: 158316644

Gene position: 5856224-5854500 (Counterclockwise)

Preceding gene: 158316645

Following gene: 158316643

Centisome position: 65.2

GC content: 68.99

Gene sequence:

>1725_bases
GTGAGAAGACGGACACGCAGCCCAGCGGCCGTCGAACAGGCTGACGGGCCGCCACGTCGCCGATCACCCGAATGCCGGCA
CCCGCGGGGCTGTCGGCGTGGCGGGGGAGGTCTCCGGGCGTTCCCGAAGCTTCCCGGCAAGAGGTTCGGAACGACCAAGG
GCGGCGTCCGGCCGACCCGGCGGCCCGCCAGCACCTACGCTTGCAGAGTGAGCGTGCGCCGGATCATGGGGACCGAGACC
GAATACGGCGTGTCCGTGCCCGGTCAACCCAACACAAACCAGATGCTGGCTTCGTCGTTGGTCGTGAACTCTTACGCCAA
CAGGGCCGCCACCGGGGGCAGTCGAGCTCGGTGGGACTTCGAGGAGGAGTCCCCCCTGCGAGACGCCCGCGGCTTCGAGC
TGCCCAGGGAGCCGGTGGTCGATCCTGGCGCGCCCGCGAACGAGGACGACCTCGGGCTGGCCAACGTCATCCTGACCAAC
GGCGCGCGCTTCTACGTCGATCATGCGCACCCCGAGTTCTCGACCCCCGAGGTGACGAACCCGCGCGACATCGTCCTCTG
GGACAAGGCGGGGGAGCGGGTGATGGCAGAGGCGGCCCGCCGTGCGCTGACTGTCCCCGGCACGATGCCCGTGCACCTGT
ACAAGAACAACACCGACAACAAGGGTGTCTCCTACGGCTGCCATGAGAACTACCTCATGGCGCGGGCGACCCCGTTCGGG
GAGATCGTGCGGCACCTGACGCCGTTCTTCGTCTCCCGCCAGGTGATATGCGGCGCCGGCCGGGTCGGCATCGGCGTGGA
CGGGCGTGAGCCCGGGTTCCAGATCAGCCAGCGCTCGGACTTCTTCGAGGTCGAGGTCGGACTGGAGACCACCCTCAAGC
GCCCCATCATCAACACCCGGGACGAGCCCCACGCCGACCCGGAGAAGTACCGGCGTCTGCACGTGATCATCGGTGACGCC
AACATGAGCGAGATCGCCACCTACCTCAAGGTGGGGATGACCTCGCTCGTCCTGGCCATGATCGAGGAGGGCTGGCTCCA
GGTCGACCTGTCGGTGGACGCGCCGGTCGCGACGCTGCGGGCGATCTCCCACGACCCCTCGCTGCGGTATCTGCTCACCC
TGCGCGACGGCCGGAAGATGACCGCCGTGCAGCTCCAGATGGAGTACTTCGAGCAGGCCCGCAAGTTCGTCGAGGACAGG
CTCGGCACGGACGTCGACCCGCAGACGGCCGACGTGCTCTCCCGGTGGGAGTCGGTGCTCGGCCGCCTCGAGGTCGACCC
GATGACCTGCTCGCGGGAGCTCGACTGGGTGGCCAAGCTGTCGATCATCGAGGGCTACCGCTCCCGTGACGCCCTGGCAT
GGGACTCGCCGCGGCTGCAGCTCGTCGACCTCCAGTACCACGACGTGCGGCCGGAGAAGGGCCTCTACAACCGCCTGGTG
GCCCGGGGCCGCTTCGACCTCCTCCTGAGTGAGGAAGAGGTCACCAGGGCGATGACGGAGCCTCCGGAGGACACCAGGGC
GTACTTCCGGGGGCGCTGCCTGGAGCTCTACCCGCAGCAGGTGGCGGCCGCTTCCTGGGACTCGGTCATCTTCGACATCG
GGCGGGACTCGCTGCAGCGGGTGCCGACCCTTGAGCCGCTGCGTGGCACCAAGGCGCACGTGGGCGAGCTGCTCGCCCGC
TGCCCCACCGCCGCCGACCTGGTGGACGCCCTGTCGGGTAACTGA

Upstream 100 bases:

>100_bases
CGTCGGGCGTCGCGCTGCCCAAGATAGTCAACGATGTTGACGCCCATCCCTGCCCGTTTTGATCTTCGAAACGGGCTTGT
GGTGGTCTCCTGCCGTCGCT

Downstream 100 bases:

>100_bases
CAGCCGGCGGAGTCTCGCGGTGGTCTCCCCGAGACTTCGCGCCGTACCGGCGCGAAGTCTCGGCGCGCCGGTGGCACCAC
CATCGACCAGCACATTTTGC

Product: hypothetical protein

Products: NA

Alternate protein names: Deamidase of protein Pup [H]

Number of amino acids: Translated: 574; Mature: 574

Protein sequence:

>574_residues
MRRRTRSPAAVEQADGPPRRRSPECRHPRGCRRGGGGLRAFPKLPGKRFGTTKGGVRPTRRPASTYACRVSVRRIMGTET
EYGVSVPGQPNTNQMLASSLVVNSYANRAATGGSRARWDFEEESPLRDARGFELPREPVVDPGAPANEDDLGLANVILTN
GARFYVDHAHPEFSTPEVTNPRDIVLWDKAGERVMAEAARRALTVPGTMPVHLYKNNTDNKGVSYGCHENYLMARATPFG
EIVRHLTPFFVSRQVICGAGRVGIGVDGREPGFQISQRSDFFEVEVGLETTLKRPIINTRDEPHADPEKYRRLHVIIGDA
NMSEIATYLKVGMTSLVLAMIEEGWLQVDLSVDAPVATLRAISHDPSLRYLLTLRDGRKMTAVQLQMEYFEQARKFVEDR
LGTDVDPQTADVLSRWESVLGRLEVDPMTCSRELDWVAKLSIIEGYRSRDALAWDSPRLQLVDLQYHDVRPEKGLYNRLV
ARGRFDLLLSEEEVTRAMTEPPEDTRAYFRGRCLELYPQQVAAASWDSVIFDIGRDSLQRVPTLEPLRGTKAHVGELLAR
CPTAADLVDALSGN

Sequences:

>Translated_574_residues
MRRRTRSPAAVEQADGPPRRRSPECRHPRGCRRGGGGLRAFPKLPGKRFGTTKGGVRPTRRPASTYACRVSVRRIMGTET
EYGVSVPGQPNTNQMLASSLVVNSYANRAATGGSRARWDFEEESPLRDARGFELPREPVVDPGAPANEDDLGLANVILTN
GARFYVDHAHPEFSTPEVTNPRDIVLWDKAGERVMAEAARRALTVPGTMPVHLYKNNTDNKGVSYGCHENYLMARATPFG
EIVRHLTPFFVSRQVICGAGRVGIGVDGREPGFQISQRSDFFEVEVGLETTLKRPIINTRDEPHADPEKYRRLHVIIGDA
NMSEIATYLKVGMTSLVLAMIEEGWLQVDLSVDAPVATLRAISHDPSLRYLLTLRDGRKMTAVQLQMEYFEQARKFVEDR
LGTDVDPQTADVLSRWESVLGRLEVDPMTCSRELDWVAKLSIIEGYRSRDALAWDSPRLQLVDLQYHDVRPEKGLYNRLV
ARGRFDLLLSEEEVTRAMTEPPEDTRAYFRGRCLELYPQQVAAASWDSVIFDIGRDSLQRVPTLEPLRGTKAHVGELLAR
CPTAADLVDALSGN
>Mature_574_residues
MRRRTRSPAAVEQADGPPRRRSPECRHPRGCRRGGGGLRAFPKLPGKRFGTTKGGVRPTRRPASTYACRVSVRRIMGTET
EYGVSVPGQPNTNQMLASSLVVNSYANRAATGGSRARWDFEEESPLRDARGFELPREPVVDPGAPANEDDLGLANVILTN
GARFYVDHAHPEFSTPEVTNPRDIVLWDKAGERVMAEAARRALTVPGTMPVHLYKNNTDNKGVSYGCHENYLMARATPFG
EIVRHLTPFFVSRQVICGAGRVGIGVDGREPGFQISQRSDFFEVEVGLETTLKRPIINTRDEPHADPEKYRRLHVIIGDA
NMSEIATYLKVGMTSLVLAMIEEGWLQVDLSVDAPVATLRAISHDPSLRYLLTLRDGRKMTAVQLQMEYFEQARKFVEDR
LGTDVDPQTADVLSRWESVLGRLEVDPMTCSRELDWVAKLSIIEGYRSRDALAWDSPRLQLVDLQYHDVRPEKGLYNRLV
ARGRFDLLLSEEEVTRAMTEPPEDTRAYFRGRCLELYPQQVAAASWDSVIFDIGRDSLQRVPTLEPLRGTKAHVGELLAR
CPTAADLVDALSGN

Specific function: Specifically catalyzes the deamidation of the C-terminal glutamine of the prokaryotic ubiquitin-like protein Pup to glutamate, thereby rendering Pup competent for conjugation. Also displays depupylase (DPUP) activity, removing conjugated Pup from target p

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Pup ligase/Pup deamidase family. Pup deamidase subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022366
- InterPro:   IPR004347 [H]

Pfam domain/function: PF03136 DUF245 [H]

EC number: NA

Molecular weight: Translated: 64133; Mature: 64133

Theoretical pI: Translated: 7.79; Mature: 7.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRRTRSPAAVEQADGPPRRRSPECRHPRGCRRGGGGLRAFPKLPGKRFGTTKGGVRPTR
CCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
RPASTYACRVSVRRIMGTETEYGVSVPGQPNTNQMLASSLVVNSYANRAATGGSRARWDF
CCCHHHHHHHHHHHHHCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
EEESPLRDARGFELPREPVVDPGAPANEDDLGLANVILTNGARFYVDHAHPEFSTPEVTN
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCCCCCCCCC
PRDIVLWDKAGERVMAEAARRALTVPGTMPVHLYKNNTDNKGVSYGCHENYLMARATPFG
CCEEEEECCCCHHHHHHHHHHEEECCCCCEEEEEECCCCCCCCCCCCCCCEEEEECCCHH
EIVRHLTPFFVSRQVICGAGRVGIGVDGREPGFQISQRSDFFEVEVGLETTLKRPIINTR
HHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCEECCCCCEEEEEECHHHHHHCCCCCCC
DEPHADPEKYRRLHVIIGDANMSEIATYLKVGMTSLVLAMIEEGWLQVDLSVDAPVATLR
CCCCCCHHHCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHH
AISHDPSLRYLLTLRDGRKMTAVQLQMEYFEQARKFVEDRLGTDVDPQTADVLSRWESVL
HHCCCCCEEEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
GRLEVDPMTCSRELDWVAKLSIIEGYRSRDALAWDSPRLQLVDLQYHDVRPEKGLYNRLV
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEECCCCEEEEEEEHHCCCCCCHHHHHHH
ARGRFDLLLSEEEVTRAMTEPPEDTRAYFRGRCLELYPQQVAAASWDSVIFDIGRDSLQR
HCCCEEEEECHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHCHHHHHHHCCHHHHHH
VPTLEPLRGTKAHVGELLARCPTAADLVDALSGN
CCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHCCC
>Mature Secondary Structure
MRRRTRSPAAVEQADGPPRRRSPECRHPRGCRRGGGGLRAFPKLPGKRFGTTKGGVRPTR
CCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
RPASTYACRVSVRRIMGTETEYGVSVPGQPNTNQMLASSLVVNSYANRAATGGSRARWDF
CCCHHHHHHHHHHHHHCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
EEESPLRDARGFELPREPVVDPGAPANEDDLGLANVILTNGARFYVDHAHPEFSTPEVTN
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCCCCCCCCC
PRDIVLWDKAGERVMAEAARRALTVPGTMPVHLYKNNTDNKGVSYGCHENYLMARATPFG
CCEEEEECCCCHHHHHHHHHHEEECCCCCEEEEEECCCCCCCCCCCCCCCEEEEECCCHH
EIVRHLTPFFVSRQVICGAGRVGIGVDGREPGFQISQRSDFFEVEVGLETTLKRPIINTR
HHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCEECCCCCEEEEEECHHHHHHCCCCCCC
DEPHADPEKYRRLHVIIGDANMSEIATYLKVGMTSLVLAMIEEGWLQVDLSVDAPVATLR
CCCCCCHHHCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHH
AISHDPSLRYLLTLRDGRKMTAVQLQMEYFEQARKFVEDRLGTDVDPQTADVLSRWESVL
HHCCCCCEEEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
GRLEVDPMTCSRELDWVAKLSIIEGYRSRDALAWDSPRLQLVDLQYHDVRPEKGLYNRLV
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEECCCCEEEEEEEHHCCCCCCHHHHHHH
ARGRFDLLLSEEEVTRAMTEPPEDTRAYFRGRCLELYPQQVAAASWDSVIFDIGRDSLQR
HCCCEEEEECHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHCHHHHHHHCCHHHHHH
VPTLEPLRGTKAHVGELLARCPTAADLVDALSGN
CCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7583123 [H]