Definition Frankia sp. EAN1pec chromosome, complete genome.
Accession NC_009921
Length 8,982,042

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The map label for this gene is pup

Identifier: 158316643

GI number: 158316643

Start: 5854061

End: 5854279

Strand: Reverse

Name: pup

Synonym: Franean1_4880

Alternate gene names: 158316643

Gene position: 5854279-5854061 (Counterclockwise)

Preceding gene: 158316644

Following gene: 158316642

Centisome position: 65.18

GC content: 63.93

Gene sequence:

>219_bases
ATGGCCCAGAGGGACACCGGTGGCGGGCAGCAGCGCACCGGCCGCCGTGATGACGAGACCGCCGAGGCCGAGGTTGAGGA
ATCGGGCGCCTCGGATCTCAAGGAAAGGCACGAGAAGCTCTCGGAGGACGTCGACTCCCTGCTCGACGAAATCGATGACG
TTCTCGAGGAGAACGCCGAGGAGTTCGTGAAGGGATACGTCCAGAAGGGCGGTCAGTAG

Upstream 100 bases:

>100_bases
CGTCGCCGGGTGTTCGTGATGGAGTGGACCTCGTACCGATCCACCGGAAGCAGTCGGCGGAGCGGGATAGTGTCGCCCCA
GGCGATCGAAGGAGGCTCCC

Downstream 100 bases:

>100_bases
GGTCTGACGCCCCGCGGCCGGTTCGGCCGGACGAGCCACATGCGCCCGTTCGGCCGGTCGCCGGGGGAGCCAACGCGGCG
ACCGGTGCCGCGGGGCCCTG

Product: hypothetical protein

Products: NA

Alternate protein names: Bacterial ubiquitin-like modifier

Number of amino acids: Translated: 72; Mature: 71

Protein sequence:

>72_residues
MAQRDTGGGQQRTGRRDDETAEAEVEESGASDLKERHEKLSEDVDSLLDEIDDVLEENAEEFVKGYVQKGGQ

Sequences:

>Translated_72_residues
MAQRDTGGGQQRTGRRDDETAEAEVEESGASDLKERHEKLSEDVDSLLDEIDDVLEENAEEFVKGYVQKGGQ
>Mature_71_residues
AQRDTGGGQQRTGRRDDETAEAEVEESGASDLKERHEKLSEDVDSLLDEIDDVLEENAEEFVKGYVQKGGQ

Specific function: Protein modifier that is covalently attached to lysine residues of substrate proteins, thereby targeting them for proteasomal degradation. The tagging system is termed pupylation

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic ubiquitin-like protein family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PUP_FRASN (A8LH55)

Other databases:

- EMBL:   CP000820
- RefSeq:   YP_001509151.1
- GeneID:   5673220
- GenomeReviews:   CP000820_GR
- KEGG:   fre:Franean1_4880
- HOGENOM:   HBG700099
- OMA:   DTGGGQQ
- ProtClustDB:   CLSK2775875
- BioCyc:   FSP1855:FRANEAN1_4880-MONOMER
- HAMAP:   MF_02106
- InterPro:   IPR008515
- TIGRFAMs:   TIGR03687

Pfam domain/function: PF05639 DUF797

EC number: NA

Molecular weight: Translated: 7998; Mature: 7866

Theoretical pI: Translated: 3.96; Mature: 3.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQRDTGGGQQRTGRRDDETAEAEVEESGASDLKERHEKLSEDVDSLLDEIDDVLEENAE
CCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EFVKGYVQKGGQ
HHHHHHHHCCCC
>Mature Secondary Structure 
AQRDTGGGQQRTGRRDDETAEAEVEESGASDLKERHEKLSEDVDSLLDEIDDVLEENAE
CCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EFVKGYVQKGGQ
HHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA