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The map label for this gene is pafA [H]

Identifier: 158316637

GI number: 158316637

Start: 5847139

End: 5848497

Strand: Reverse

Name: pafA [H]

Synonym: Franean1_4874

Alternate gene names: 158316637

Gene position: 5848497-5847139 (Counterclockwise)

Preceding gene: 158316638

Following gene: 158316636

Centisome position: 65.11

GC content: 68.73

Gene sequence:

>1359_bases
GTGGATCGCCGCATCTTCGGGCTGGAGAACGAGTACGGCGTCACCTGCGTGTTCCGCGGGCAGCGAAGGCTGTCGCCGGA
CGAGGTGGCCCGTTACCTGTTCCGACGCGTCGTGTCCTGGGGCCGTAGCAGCAACGTGTTCCTCAAGAACGGCGCCCGGC
TCTACCTGGACGTCGGCAGCCACCCGGAGTACGCCACCCCCGAGTGCGACTCCGTGCCCGATCTGGTGACGCACGACAAG
GCGGGCGAGCGGATCCTCGAGGGCCTGCTCGTCGAGGCGGAGCGCCGGCTGCGCGAAGAGGGCATCGCCGGGGACATCCA
CCTGTTCAAGAACAACACCGACTCGGCGGGGAACAGCTACGGCTGCCACGAGAACTACCTCGTGGGGCGGCACGGCGAGT
TCAGCCGCCTGGCGGACGTGCTGGTCCCGTTCCTGGTCAGCCGGCAGATCCTGTGCGGCGCGGGCAAGGTCCTTCAGACC
CCGCGCGGGGCGGTGTACTGCATCTCCCAGCGCGCCGAGCACATCTGGGAGTCGGTGTCGTCGGCGACGACCCGCTCGCG
TCCGATCATCAACACCCGCGACGAGCCGCACGCGGACGCCGAGCGCTTCCGTCGGCTGCACGTCATCGTCGGTGACTCGA
ACATGAGCGAGACGACGATGCTGCTCAAGCTGGGCTCCACCGACCTGGTGCTCCGCATGATCGAGGCGGGGGTCGTCCTG
CGCGACATGTCGCTGGAGAACCCGATCCGGGCGATCCGCGAGGTGTCCCACGACATGACCTGCCAGCGCCGGATCAAGCT
GGCGAACGGCCGTGAGGTGAGCGCGCTGGACATCCAGCGCGAGTACTACTCGAAGGCGGTCGAGTTCGTCGAGCGGCGCG
GCGGGGACGCCGTGGCGAAGCGGGTGCTCGACCTGTGGGGCCGCACCCTGCTCGCGATCGAGACCGAGGACCTGGAGCTG
GTCGCCCGGGAGATCGACTGGGTCACCAAGTTCGTCCTCATCGACCGCTTCCGGCTCAAGCACGGGCTGTCGCTGGCGTC
GCCGCGGGTGGCCGAGCTCGACCTGAAGTACCACGACATCCACCGTGACCGCGGGCTCTACTACCGGATGGAGCGGGCCG
GCCTGGTCGAGCGGGTCACCCGCGACCTCGACATCTTCGAGGCGAAGTCGGTGCCGCCGCAGACCACCCGGGCCCGGCTG
CGCGGGGAGTTCATCAAGCGGGCGCAGGAGAAGCGGCGCGACTTCACCGTCGACTGGGTGCACCTCAAGCTCAACGACCA
GGCCCAGCGCACGGTGCTGTGCAAGGACCCGTTCCGGTCGGTGGACGACCGCGTCGACAAGCTGATCGCGAGCATGTAG

Upstream 100 bases:

>100_bases
GTCGCCGCCGGGCTGGTCGTGCGCCCCCGGGAGGCCACAGCTGAGACCAGCCCGAGCGTCTCCCCACGCGAAGGCGGGTT
CCGAGCATAGGGTCGGTCTC

Downstream 100 bases:

>100_bases
GCCGCGCCGGCCCGGCCGGCCGGTGTCGTGCCGAAGCACGGAGGCTTGTCCTCTACAGTCTGACTCCACAGGTTCAGGCA
GCTCGAGCGCGTGGCGCCGG

Product: hypothetical protein

Products: NA

Alternate protein names: Proteasome accessory factor A; Pup-conjugating enzyme [H]

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MDRRIFGLENEYGVTCVFRGQRRLSPDEVARYLFRRVVSWGRSSNVFLKNGARLYLDVGSHPEYATPECDSVPDLVTHDK
AGERILEGLLVEAERRLREEGIAGDIHLFKNNTDSAGNSYGCHENYLVGRHGEFSRLADVLVPFLVSRQILCGAGKVLQT
PRGAVYCISQRAEHIWESVSSATTRSRPIINTRDEPHADAERFRRLHVIVGDSNMSETTMLLKLGSTDLVLRMIEAGVVL
RDMSLENPIRAIREVSHDMTCQRRIKLANGREVSALDIQREYYSKAVEFVERRGGDAVAKRVLDLWGRTLLAIETEDLEL
VAREIDWVTKFVLIDRFRLKHGLSLASPRVAELDLKYHDIHRDRGLYYRMERAGLVERVTRDLDIFEAKSVPPQTTRARL
RGEFIKRAQEKRRDFTVDWVHLKLNDQAQRTVLCKDPFRSVDDRVDKLIASM

Sequences:

>Translated_452_residues
MDRRIFGLENEYGVTCVFRGQRRLSPDEVARYLFRRVVSWGRSSNVFLKNGARLYLDVGSHPEYATPECDSVPDLVTHDK
AGERILEGLLVEAERRLREEGIAGDIHLFKNNTDSAGNSYGCHENYLVGRHGEFSRLADVLVPFLVSRQILCGAGKVLQT
PRGAVYCISQRAEHIWESVSSATTRSRPIINTRDEPHADAERFRRLHVIVGDSNMSETTMLLKLGSTDLVLRMIEAGVVL
RDMSLENPIRAIREVSHDMTCQRRIKLANGREVSALDIQREYYSKAVEFVERRGGDAVAKRVLDLWGRTLLAIETEDLEL
VAREIDWVTKFVLIDRFRLKHGLSLASPRVAELDLKYHDIHRDRGLYYRMERAGLVERVTRDLDIFEAKSVPPQTTRARL
RGEFIKRAQEKRRDFTVDWVHLKLNDQAQRTVLCKDPFRSVDDRVDKLIASM
>Mature_452_residues
MDRRIFGLENEYGVTCVFRGQRRLSPDEVARYLFRRVVSWGRSSNVFLKNGARLYLDVGSHPEYATPECDSVPDLVTHDK
AGERILEGLLVEAERRLREEGIAGDIHLFKNNTDSAGNSYGCHENYLVGRHGEFSRLADVLVPFLVSRQILCGAGKVLQT
PRGAVYCISQRAEHIWESVSSATTRSRPIINTRDEPHADAERFRRLHVIVGDSNMSETTMLLKLGSTDLVLRMIEAGVVL
RDMSLENPIRAIREVSHDMTCQRRIKLANGREVSALDIQREYYSKAVEFVERRGGDAVAKRVLDLWGRTLLAIETEDLEL
VAREIDWVTKFVLIDRFRLKHGLSLASPRVAELDLKYHDIHRDRGLYYRMERAGLVERVTRDLDIFEAKSVPPQTTRARL
RGEFIKRAQEKRRDFTVDWVHLKLNDQAQRTVLCKDPFRSVDDRVDKLIASM

Specific function: Catalyzes the covalent attachment of the prokaryotic ubiquitin-like protein modifier Pup to the proteasomal substrate proteins, thereby targeting them for proteasomal degradation. This tagging system is termed pupylation. The ligation reaction involves th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Pup ligase/Pup deamidase family. Pup- conjugating enzyme subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022279
- InterPro:   IPR004347 [H]

Pfam domain/function: PF03136 DUF245 [H]

EC number: NA

Molecular weight: Translated: 51886; Mature: 51886

Theoretical pI: Translated: 8.64; Mature: 8.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRRIFGLENEYGVTCVFRGQRRLSPDEVARYLFRRVVSWGRSSNVFLKNGARLYLDVGS
CCCCEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCEEEEEECC
HPEYATPECDSVPDLVTHDKAGERILEGLLVEAERRLREEGIAGDIHLFKNNTDSAGNSY
CCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCC
GCHENYLVGRHGEFSRLADVLVPFLVSRQILCGAGKVLQTPRGAVYCISQRAEHIWESVS
CCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCEEEEHHHHHHHHHHHHH
SATTRSRPIINTRDEPHADAERFRRLHVIVGDSNMSETTMLLKLGSTDLVLRMIEAGVVL
HHHHCCCCCCCCCCCCCHHHHHCCEEEEEECCCCCCCEEEEEEECCHHHHHHHHHCCCEE
RDMSLENPIRAIREVSHDMTCQRRIKLANGREVSALDIQREYYSKAVEFVERRGGDAVAK
ECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHCCCHHHHH
RVLDLWGRTLLAIETEDLELVAREIDWVTKFVLIDRFRLKHGLSLASPRVAELDLKYHDI
HHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCHHHHH
HRDRGLYYRMERAGLVERVTRDLDIFEAKSVPPQTTRARLRGEFIKRAQEKRRDFTVDWV
HCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEE
HLKLNDQAQRTVLCKDPFRSVDDRVDKLIASM
EEEECCCCCCEEEECCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDRRIFGLENEYGVTCVFRGQRRLSPDEVARYLFRRVVSWGRSSNVFLKNGARLYLDVGS
CCCCEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCEEEEEECC
HPEYATPECDSVPDLVTHDKAGERILEGLLVEAERRLREEGIAGDIHLFKNNTDSAGNSY
CCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCC
GCHENYLVGRHGEFSRLADVLVPFLVSRQILCGAGKVLQTPRGAVYCISQRAEHIWESVS
CCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCEEEEHHHHHHHHHHHHH
SATTRSRPIINTRDEPHADAERFRRLHVIVGDSNMSETTMLLKLGSTDLVLRMIEAGVVL
HHHHCCCCCCCCCCCCCHHHHHCCEEEEEECCCCCCCEEEEEEECCHHHHHHHHHCCCEE
RDMSLENPIRAIREVSHDMTCQRRIKLANGREVSALDIQREYYSKAVEFVERRGGDAVAK
ECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHCCCHHHHH
RVLDLWGRTLLAIETEDLELVAREIDWVTKFVLIDRFRLKHGLSLASPRVAELDLKYHDI
HHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCHHHHH
HRDRGLYYRMERAGLVERVTRDLDIFEAKSVPPQTTRARLRGEFIKRAQEKRRDFTVDWV
HCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEE
HLKLNDQAQRTVLCKDPFRSVDDRVDKLIASM
EEEECCCCCCEEEECCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA