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The map label for this gene is cotA [H]

Identifier: 158316580

GI number: 158316580

Start: 5749661

End: 5751199

Strand: Reverse

Name: cotA [H]

Synonym: Franean1_4816

Alternate gene names: 158316580

Gene position: 5751199-5749661 (Counterclockwise)

Preceding gene: 158316581

Following gene: 158316576

Centisome position: 64.03

GC content: 68.49

Gene sequence:

>1539_bases
ATGAGTCGTCTGCTGAGCCGACGAAACGTGCTGGGCGCCTGGGTCGCCGCGACCGCGGCGGGCACCGTCCGGCTGGCCAC
GGCCGGCGGCGGCGGTGACGGCGGTGCCTCGCTGGCCTCCGCCAGTGCGTCAACCGACCACGCAGCCCATCTCGGTCACT
CCGGAACCGTCGAGACGGTCGCGAACGAGGTGACCGCGGCCCCGCCGGTGACGCCGTTCTCCGTTCCGCTGCCGGTCCCG
CCGACGCTCGCGCCGACGTCCACAACCGCGGACACGGACTTCTACGAGATAACCAGCCGGACCGCGGCGGAGGAGATCCT
GCCCGGTTTCTCCACCGAGATCCACGGCTACGGCGGGAGCTATGTCGGGCCGACGATAAAGGCGAAGGTCGGCCGCAAGG
TTGTTGTCCGGCACACCAACCAGCTGCCCCACCCGACCGCCGTCCACCTGCACGGGGCACATGTCCCGGCGTCCAGCGAC
GGCTTTCCGACCGATGTCATCGACACCGGTGCGACGAAGACCTACGAGTACCCGAACAACCAGCCGGCCGCGACCCTGTG
GTATCACGACCATGCGCATCATCACGAGACCGAGAACATCTACCGGGGCCTGCACGGCTTCTACCTGCTGGAGGATCCGG
CCGAGGCGGCACTGAACCTTCCGAGCGGGGAGTTCGACATTCCCGTCCTGCTGTCCGACGCCAACATCGACGCCGACGGC
CAGCTCGTCTTCATCAGCCTGGATGAGTACGGACGGACGACGCTGCTGACGAACGGCCGCCCCGCCCCGTACTTTCAGGT
GCAGGCCCGCCGCTACCGGTTCCGGTTCCTGAACGCCAGCAACATGCGGGTGTTCAACCTGGTACTGGGCCGCCAGGAGA
GGTTCACTCAGATCGCGGGGGACGGCGGCCTGCTGCCGGCGCCGGCGACCGTCGACAAGGTCCTGCTCAGTGCCGGTGAG
CGGGCCGAGATCGTCATCGACTTCTCCAGGTACGCGCCCGGCACCCAGCTCGTCCTGAACAACACCCGGAACCTCAACGA
TCCGAGCCGGCCGGTCGCCCTGCCCGTGCTGCGCTTCGACGTGGTCGCCCGGGTGGGGCAGGACACCAGCGCCGTCCCGG
CCACCCTGCGAGCGCATCCGCCGCTGCCCGCCGCGACCCGGGTCCGGGACGTGGTCCTCTCGTACCACACCGCGGCCTTC
CCGTTCGCGATCAACGGGATGCTGTTCGACCCCAACCGCGTCGACTACACCATTCGCCGCGGCAGCACCGAGATCTGGCG
GATCACCAACCCGGTCACCCCGGTGGCGGTCTACCACAACCTGCACCTGCATCTGGTGCAGTTCCGGATCCTGGACCGCG
ACGGCGTACCGCCCGGTCCGGCCGAGTCGGGCTGGAAGGACACCGTGTTCGTGGCGCCGGGCGAGACGGTCCGCATCCAG
GCGACGTTCACCGAGTATCTCGGTAAGTACCTCTACCACTGCCACGTCCAGGATCACGCCGGCACCGGGATGATGGCACT
GTTCGAGACTGTGGCCTGA

Upstream 100 bases:

>100_bases
GGGAGGTCTTAGGGGTAGGCATCGGCATGTGTGCCCAGGGATGGTGCTGGCCGTCCGGCGACTGTCCGGAGCGCACCGAC
CATGGTGGAGGCAATCGCTG

Downstream 100 bases:

>100_bases
GAACGGGCGCGGGCGTGGGCCGCGGCCCCGGTGGATCTCCACCGGGGCCGCGGCCCACGCCGTGCGGCGGAAGCCGATTT
CGTGCGGCTCGGCACCTCAG

Product: Bilirubin oxidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 512; Mature: 511

Protein sequence:

>512_residues
MSRLLSRRNVLGAWVAATAAGTVRLATAGGGGDGGASLASASASTDHAAHLGHSGTVETVANEVTAAPPVTPFSVPLPVP
PTLAPTSTTADTDFYEITSRTAAEEILPGFSTEIHGYGGSYVGPTIKAKVGRKVVVRHTNQLPHPTAVHLHGAHVPASSD
GFPTDVIDTGATKTYEYPNNQPAATLWYHDHAHHHETENIYRGLHGFYLLEDPAEAALNLPSGEFDIPVLLSDANIDADG
QLVFISLDEYGRTTLLTNGRPAPYFQVQARRYRFRFLNASNMRVFNLVLGRQERFTQIAGDGGLLPAPATVDKVLLSAGE
RAEIVIDFSRYAPGTQLVLNNTRNLNDPSRPVALPVLRFDVVARVGQDTSAVPATLRAHPPLPAATRVRDVVLSYHTAAF
PFAINGMLFDPNRVDYTIRRGSTEIWRITNPVTPVAVYHNLHLHLVQFRILDRDGVPPGPAESGWKDTVFVAPGETVRIQ
ATFTEYLGKYLYHCHVQDHAGTGMMALFETVA

Sequences:

>Translated_512_residues
MSRLLSRRNVLGAWVAATAAGTVRLATAGGGGDGGASLASASASTDHAAHLGHSGTVETVANEVTAAPPVTPFSVPLPVP
PTLAPTSTTADTDFYEITSRTAAEEILPGFSTEIHGYGGSYVGPTIKAKVGRKVVVRHTNQLPHPTAVHLHGAHVPASSD
GFPTDVIDTGATKTYEYPNNQPAATLWYHDHAHHHETENIYRGLHGFYLLEDPAEAALNLPSGEFDIPVLLSDANIDADG
QLVFISLDEYGRTTLLTNGRPAPYFQVQARRYRFRFLNASNMRVFNLVLGRQERFTQIAGDGGLLPAPATVDKVLLSAGE
RAEIVIDFSRYAPGTQLVLNNTRNLNDPSRPVALPVLRFDVVARVGQDTSAVPATLRAHPPLPAATRVRDVVLSYHTAAF
PFAINGMLFDPNRVDYTIRRGSTEIWRITNPVTPVAVYHNLHLHLVQFRILDRDGVPPGPAESGWKDTVFVAPGETVRIQ
ATFTEYLGKYLYHCHVQDHAGTGMMALFETVA
>Mature_511_residues
SRLLSRRNVLGAWVAATAAGTVRLATAGGGGDGGASLASASASTDHAAHLGHSGTVETVANEVTAAPPVTPFSVPLPVPP
TLAPTSTTADTDFYEITSRTAAEEILPGFSTEIHGYGGSYVGPTIKAKVGRKVVVRHTNQLPHPTAVHLHGAHVPASSDG
FPTDVIDTGATKTYEYPNNQPAATLWYHDHAHHHETENIYRGLHGFYLLEDPAEAALNLPSGEFDIPVLLSDANIDADGQ
LVFISLDEYGRTTLLTNGRPAPYFQVQARRYRFRFLNASNMRVFNLVLGRQERFTQIAGDGGLLPAPATVDKVLLSAGER
AEIVIDFSRYAPGTQLVLNNTRNLNDPSRPVALPVLRFDVVARVGQDTSAVPATLRAHPPLPAATRVRDVVLSYHTAAFP
FAINGMLFDPNRVDYTIRRGSTEIWRITNPVTPVAVYHNLHLHLVQFRILDRDGVPPGPAESGWKDTVFVAPGETVRIQA
TFTEYLGKYLYHCHVQDHAGTGMMALFETVA

Specific function: Involved in brown pigmentation during sporogenesis [H]

COG id: COG2132

COG function: function code Q; Putative multicopper oxidases

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To S.antibioticus phenoxazinone synthase (phsA) [H]

Homologues:

Organism=Escherichia coli, GI1786314, Length=511, Percent_Identity=30.3326810176125, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI1789394, Length=422, Percent_Identity=31.7535545023697, Blast_Score=174, Evalue=1e-44,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001117
- InterPro:   IPR011706
- InterPro:   IPR011707
- InterPro:   IPR008972 [H]

Pfam domain/function: PF00394 Cu-oxidase; PF07731 Cu-oxidase_2; PF07732 Cu-oxidase_3 [H]

EC number: NA

Molecular weight: Translated: 55345; Mature: 55214

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: PS00079 MULTICOPPER_OXIDASE1 ; PS00080 MULTICOPPER_OXIDASE2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLLSRRNVLGAWVAATAAGTVRLATAGGGGDGGASLASASASTDHAAHLGHSGTVETV
CCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHCCCCCCCHHHHCCCCCCHHHH
ANEVTAAPPVTPFSVPLPVPPTLAPTSTTADTDFYEITSRTAAEEILPGFSTEIHGYGGS
HHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEECCCCC
YVGPTIKAKVGRKVVVRHTNQLPHPTAVHLHGAHVPASSDGFPTDVIDTGATKTYEYPNN
CCCCEEEECCCCEEEEEECCCCCCCEEEEEECCCCCCCCCCCCCHHCCCCCCEEEECCCC
QPAATLWYHDHAHHHETENIYRGLHGFYLLEDPAEAALNLPSGEFDIPVLLSDANIDADG
CCCEEEEEECCCCCCCHHHHHHCCCCEEEEECHHHHHEECCCCCCCEEEEEECCCCCCCC
QLVFISLDEYGRTTLLTNGRPAPYFQVQARRYRFRFLNASNMRVFNLVLGRQERFTQIAG
EEEEEEECCCCCEEEEECCCCCCEEEEEEEEEEEEEECCCCCEEEEEECCCHHHHHEECC
DGGLLPAPATVDKVLLSAGERAEIVIDFSRYAPGTQLVLNNTRNLNDPSRPVALPVLRFD
CCCCCCCCHHHHHHHHCCCCCEEEEEEEHHCCCCCEEEEECCCCCCCCCCCEEEHHHHHH
VVARVGQDTSAVPATLRAHPPLPAATRVRDVVLSYHTAAFPFAINGMLFDPNRVDYTIRR
HHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHEECCEEECCEEECCCCEEEEEEC
GSTEIWRITNPVTPVAVYHNLHLHLVQFRILDRDGVPPGPAESGWKDTVFVAPGETVRIQ
CCCEEEEECCCCCEEEEEECCEEEEEEEEEECCCCCCCCCCCCCCCCEEEECCCCEEEEE
ATFTEYLGKYLYHCHVQDHAGTGMMALFETVA
EHHHHHHHHHEEEEEECCCCCCCHHHHHHHCC
>Mature Secondary Structure 
SRLLSRRNVLGAWVAATAAGTVRLATAGGGGDGGASLASASASTDHAAHLGHSGTVETV
CHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHCCCCCCCHHHHCCCCCCHHHH
ANEVTAAPPVTPFSVPLPVPPTLAPTSTTADTDFYEITSRTAAEEILPGFSTEIHGYGGS
HHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEECCCCC
YVGPTIKAKVGRKVVVRHTNQLPHPTAVHLHGAHVPASSDGFPTDVIDTGATKTYEYPNN
CCCCEEEECCCCEEEEEECCCCCCCEEEEEECCCCCCCCCCCCCHHCCCCCCEEEECCCC
QPAATLWYHDHAHHHETENIYRGLHGFYLLEDPAEAALNLPSGEFDIPVLLSDANIDADG
CCCEEEEEECCCCCCCHHHHHHCCCCEEEEECHHHHHEECCCCCCCEEEEEECCCCCCCC
QLVFISLDEYGRTTLLTNGRPAPYFQVQARRYRFRFLNASNMRVFNLVLGRQERFTQIAG
EEEEEEECCCCCEEEEECCCCCCEEEEEEEEEEEEEECCCCCEEEEEECCCHHHHHEECC
DGGLLPAPATVDKVLLSAGERAEIVIDFSRYAPGTQLVLNNTRNLNDPSRPVALPVLRFD
CCCCCCCCHHHHHHHHCCCCCEEEEEEEHHCCCCCEEEEECCCCCCCCCCCEEEHHHHHH
VVARVGQDTSAVPATLRAHPPLPAATRVRDVVLSYHTAAFPFAINGMLFDPNRVDYTIRR
HHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHEECCEEECCEEECCCCEEEEEEC
GSTEIWRITNPVTPVAVYHNLHLHLVQFRILDRDGVPPGPAESGWKDTVFVAPGETVRIQ
CCCEEEEECCCCCEEEEEECCEEEEEEEEEECCCCCCCCCCCCCCCCEEEECCCCEEEEE
ATFTEYLGKYLYHCHVQDHAGTGMMALFETVA
EHHHHHHHHHEEEEEECCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969499; 9455482; 9384377; 2821284; 3135411 [H]